Mycobacterium phage Inca
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Detailed Information for Phage Inca
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByCourtney Davies
Year Found2015
Location FoundAuckland, New Zealand
Finding InstitutionMassey University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates36.671139 S, 174.662436 E Map
Discovery NotesSoil sample collected 27th August 2015 at 9.57am.
The ambient temperature was 13°C. No wind and a light drizzle of rain at time of collection. A small hole was dug and the soil collected 2cm deep underneath a rotting silage bale. The silage had not been grazed by cattle since April 2015 and had started to decompose. The site was fenced off with an electric fence above and was 5m east of a concreted driveway on a flat rural/lifestyle property in Dairy Flat.
Naming NotesInca is the name of my purebred Ayrshire stud. I chose the same name as it represents my cows whom are very special to me (the initial name stemmed from the successful Inca Empire). Inca can also be roughly translated to "ruler" or "lord". So not only does Inca Phage have a fascinating name, it is also an intriguing phage full of potential!
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedNov 29, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2299
Genome length (bp)74070
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content62.8%
Fasta file available?Yes: Download fasta file
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusAwaiting_QC
Plaque NotesThe morphology was very interesting with this plaque. Initially there were lytic plaques present between 24 and 72 hours of incubation, however after 10 days of continued incubation at 37°C these changed and it was clear that the once lytic plaque now had a turbid ring around it and a further lytic ring beyond. From there on there was no change. Plaques were approximately 2mm initially and then developed into 5mm plaques.
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.0 x 10^9 pfu/mL
Date of SEA Lysate Titering Sep 18, 2015
Pitt Freezer Box# 19
Pitt Freezer Box Grid# C11
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload