Recently Added Phages

Miracyn NickNutty Pear Benito NeumannU

Recently Modified Phages

Bobalouis Airam Wakko Kalb97 Ness

Recently Finished Phages

ArMaWen (EK) ThulaThula (None) Scuttle (AK) Ajay (A1) Arby (AN)
Mycobacterium phage Inca
Know something about this phage that we don't? Modify its data.
Detailed Information for Phage Inca
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByCourtney Davies
Year Found2015
Location FoundAuckland, New Zealand
Finding InstitutionMassey University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates36.671139 S, 174.662436 E Map
Discovery NotesSoil sample collected 27th August 2015 at 9.57am.
The ambient temperature was 13°C. No wind and a light drizzle of rain at time of collection. A small hole was dug and the soil collected 2cm deep underneath a rotting silage bale. The silage had not been grazed by cattle since April 2015 and had started to decompose. The site was fenced off with an electric fence above and was 5m east of a concreted driveway on a flat rural/lifestyle property in Dairy Flat.
Naming NotesInca is the name of my purebred Ayrshire stud. I chose the same name as it represents my cows whom are very special to me (the initial name stemmed from the successful Inca Empire). Inca can also be roughly translated to "ruler" or "lord". So not only does Inca Phage have a fascinating name, it is also an intriguing phage full of potential!
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedNov 29, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2299
Genome length (bp)74070
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content62.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesThe morphology was very interesting with this plaque. Initially there were lytic plaques present between 24 and 72 hours of incubation, however after 10 days of continued incubation at 37°C these changed and it was clear that the once lytic plaque now had a turbid ring around it and a further lytic ring beyond. From there on there was no change. Plaques were approximately 2mm initially and then developed into 5mm plaques.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

Inca_1

Inca_2

Inca_3

Inca_4

Inca_5

Inca_6
(terminase small subunit)

Inca_7

Inca_8
(terminase large subunit)

Inca_9

Inca_10
(portal protein)

Inca_11
(capsid maturation protease)

Inca_12
(major capsid protein)

Inca_13

Inca_14
(head-to-tail connector complex protein)

Inca_15
(head-to-tail connector complex protein)

Inca_16

Inca_17
(head-to-tail connector complex protein)

Inca_18
(major tail protein)

Inca_19
(tail assembly chaperone)

Inca_20
(tail assembly chaperone)

Inca_21
(tape measure protein)

Inca_22
(minor tail protein)

Inca_23
(minor tail protein)

Inca_24
(minor tail protein)

Inca_25
(minor tail protein)

Inca_26
(D-ala-D-ala carboxypeptidase)

Inca_27
(minor tail protein)

Inca_28

Inca_29
(helix-turn-helix DNA binding domain protein)

Inca_30

Inca_31
(lysin A)

Inca_32
(holin)

Inca_33

Inca_34
(lysin B)

Inca_35

Inca_36
(NrdH-like glutaredoxin)

Inca_37
(DNA bridging protein)

Inca_38

Inca_39

Inca_40

Inca_41

Inca_42

Inca_43

Inca_44

Inca_45

Inca_46

Inca_47

Inca_48
(membrane protein)

Inca_49
(integrase)

Inca_50

Inca_51
(membrane protein)

Inca_52
(immunity repressor)

Inca_53

Inca_54

Inca_55
(pentapeptide repeat protein)

Inca_56

Inca_57

Inca_58

Inca_59

Inca_60

Inca_61

Inca_62

Inca_63

Inca_64
(exonuclease)

Inca_65

Inca_66
(ERF family ssDNA binding protein)

Inca_67

Inca_68

Inca_69

Inca_70

Inca_71

Inca_72

Inca_73
(DprA-like DNA processing chain A)

Inca_74
(HNH endonuclease)

Inca_75

Inca_76

Inca_77

Inca_78
(DnaB-like dsDNA helicase)

Inca_79

Inca_80
(WhiB family transcription factor)

Inca_81

Inca_82

Inca_83
(holliday junction resolvase)

Inca_84

Inca_85
(polynucleotide kinase)

Inca_86

Inca_87

Inca_88
(RNA ligase)

Inca_89
(helix-turn-helix DNA binding domain protein)

Inca_90
(WhiB family transcription factor)

Inca_91

Inca_92

Inca_93

Inca_94

Inca_95

Inca_96
(endonuclease VII)

Inca_97
(ssDNA binding protein)

Inca_98
(ClpP-like protease)

Inca_99

Inca_100

Inca_101

Inca_102
(helix-turn-helix DNA binding domain protein)

Inca_103
(HNH endonuclease)

Inca_106

Inca_107

Inca_108

Inca_109

Inca_110
(DNA polymerase III subunit)

Inca_111

Inca_112

Inca_113

Inca_114

Inca_115
(RecA-like DNA recombinase)

Inca_116

Inca_117

Inca_118

Inca_119

Inca_120

Inca_121

Inca_122

Inca_123

Inca_124

Inca_125

Inca_126

Inca_127
(helix-turn-helix DNA binding protein)

Inca_128

Inca_129

Inca_130

Inca_131

Inca_132

Inca_133

Inca_134

Inca_135

Inca_136

Inca_137

Inca_138

Inca_139

Inca_140

Inca_141

Inca_142

Inca_143

Inca_144

Inca_145

Inca_146

Inca_147
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH576956
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.0 x 10^9 pfu/mL
Date of SEA Lysate Titering Sep 18, 2015
Pitt Freezer Box# 19
Pitt Freezer Box Grid# C11
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload