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Mycobacterium phage Tuco
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Detailed Information for Phage Tuco
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByEddy Santos
Year Found2013
Location FoundNyack, NY United States of America
Finding InstitutionNyack College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates41.081909 N, 73.927521 W Map
Discovery NotesHee Jae Ko found this soil sample by a tree by Simpson Hall at Nyack College. This soil sample had two types of phages named Tuco and Beesknees in it. Eddy Santos worked on Tuco while Beesknees was worked on by Greg Mastrangelo.
Naming NotesTuco was a character in the TV show series Breaking Bad
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 3, 2014
Sequencing FacilityNC State Genomic Sciences Laboratory
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage4765
Genome length (bp)76944
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content63.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Other Cluster Members
Plaque NotesA lot of small and clear plaques.
MorphotypeSiphoviridae
Final annotation complete?No
Has been Phamerated?Yes
Gene List

Click to View

Tuco_1

Tuco_2

Tuco_3

Tuco_4

Tuco_5

Tuco_6
(terminase, small subunit)

Tuco_7
(membrane domain protein)

Tuco_8

Tuco_9
(terminase, large subunit)

Tuco_10

Tuco_11
(portal protein)

Tuco_12
(capsid maturation protease)

Tuco_13
(major capsid protein)

Tuco_14
(head-to-tail connector protein)

Tuco_15
(head-to-tail connector protein)

Tuco_16
(head-to-tail connector protein)

Tuco_17
(head-to-tail connector protein)

Tuco_18
(head-to-tail connector protein)

Tuco_19
(major tail protein)

Tuco_20
(tail assembly chaperone)

Tuco_21
(tail assembly chaperone)

Tuco_22
(HNH endonuclease)

Tuco_23
(tape measure protein)

Tuco_24
(minor tail protein)

Tuco_25
(minor tail protein)

Tuco_26
(minor tail protein)

Tuco_27
(minor tail protein)

Tuco_28
(minor tail protein, D-ala-D-ala-carboxypeptidase)

Tuco_29

Tuco_30

Tuco_31
(HTH DNA binding protein)

Tuco_32
(membrane domain protein)

Tuco_33
(lysin A)

Tuco_34
(holin)

Tuco_35

Tuco_36
(lysin B)

Tuco_37
(membrane domain protein)

Tuco_38
(NrdH glutaredoxin)

Tuco_39
(HNH endonuclease)

Tuco_40

Tuco_41
(Lsr2 DNA bridging protein)

Tuco_42

Tuco_43

Tuco_44

Tuco_45

Tuco_46

Tuco_47

Tuco_48

Tuco_49

Tuco_50

Tuco_51

Tuco_52
(membrane domain protein)

Tuco_53
(integrase, (Y-int))

Tuco_54

Tuco_55
(membrane domain protein)

Tuco_56
(immunity repressor)

Tuco_57

Tuco_58
(membrane domain protein)

Tuco_59
(pentapeptide repeat protein)

Tuco_60

Tuco_61
(DNA binding protein)

Tuco_62

Tuco_63

Tuco_64

Tuco_65

Tuco_66

Tuco_67

Tuco_68

Tuco_69

Tuco_70
(ssDNA binding protein, ERF family)

Tuco_71

Tuco_72

Tuco_73

Tuco_74

Tuco_75
(membrane domain protein)

Tuco_76

Tuco_77

Tuco_78
(HNH endonuclease)

Tuco_79

Tuco_80

Tuco_81

Tuco_82
(DnaB dsDNA helicase)

Tuco_83

Tuco_84
(WhiB family transcription factor)

Tuco_85

Tuco_86

Tuco_87
(Hjc holiday junction resolvase)

Tuco_88
(membrane domain protein)

Tuco_89
(polynucleotide kinase)

Tuco_90

Tuco_91
(membrane domain protein)

Tuco_92
(RNA ligase)

Tuco_93
(HTH DNA binding protein)

Tuco_94
(WhiB family transcription factor)

Tuco_95

Tuco_96

Tuco_97

Tuco_98

Tuco_99

Tuco_100
(DNA methylase)

Tuco_101
(ssDNA binding protein)

Tuco_102
(ClpP protease)

Tuco_103

Tuco_104

Tuco_105

Tuco_106
(HTH DNA binding protein)

Tuco_107
(HNH endonuclease)

Tuco_110

Tuco_111

Tuco_112
(hydrolase)

Tuco_113
(MazG nucleotide pyrophosphohydrolase)

Tuco_114

Tuco_115
(DnaQ exonuclease (DNA polymerase III subunit))

Tuco_116

Tuco_117

Tuco_118

Tuco_119

Tuco_120
(RecA DNA recombinase)

Tuco_121

Tuco_122

Tuco_123

Tuco_124

Tuco_125

Tuco_126

Tuco_127

Tuco_128

Tuco_129

Tuco_130

Tuco_131

Tuco_132

Tuco_133
(helix-turn-helix DNA binding domain)

Tuco_134

Tuco_135

Tuco_136

Tuco_137

Tuco_138

Tuco_139

Tuco_140

Tuco_141

Tuco_142

Tuco_143

Tuco_144

Tuco_145

Tuco_146

Tuco_147

Tuco_148

Tuco_149

Tuco_150
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKX817173
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE
Available Files
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload