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Mycobacterium phage Toto
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Detailed Information for Phage Toto
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByLucia Barker
Year Found2008
Location FoundAshburn, VA USA
Finding InstitutionHoward Hughes Medical Institute
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates39.0702 N, 77.4639 W Map
Discovery NotesFrom soil from JFRC Roof - under a tree
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 31, 2011
Genome length (bp)75933
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content63.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesVariable morphology - ~2mm/irregularly shaped turbid plaques, roundish clear plaques after 24 hour incubation at 37°C on 7H10 plates.
MorphotypeSiphoviridae
Number of Genes135
Number of tRNAs2
Has been Phamerated?Yes
Gene List

Click to View

Toto_1

Toto_2

Toto_3

Toto_4

Toto_5

Toto_6
(terminase small subunit)

Toto_7

Toto_8

Toto_9
(terminase large subunit)

Toto_10

Toto_11
(portal protein)

Toto_12

Toto_13
(major capsid protein)

Toto_14

Toto_15

Toto_16

Toto_17

Toto_18

Toto_19
(major tail subunit)

Toto_20
(tail assembly chaperone)

Toto_21
(tail assembly chaperone)

Toto_22
(tapemeasure protein)

Toto_23
(minor tail protein)

Toto_24
(minor tail protein)

Toto_25
(minor tail protein)

Toto_26
(minor tail protein)

Toto_27
(minor tail protein)

Toto_28

Toto_29

Toto_30

Toto_31

Toto_32
(lysin A)

Toto_33
(holin)

Toto_34

Toto_35
(lysin B)

Toto_36

Toto_37
(NrdH)

Toto_38
(HNH endonuclease domain protein)

Toto_39
(Lsr2)

Toto_40

Toto_41

Toto_42

Toto_43

Toto_44

Toto_45

Toto_46

Toto_47

Toto_48

Toto_49

Toto_50

Toto_51
(integrase)

Toto_52

Toto_53

Toto_54

Toto_55

Toto_56

Toto_57

Toto_58

Toto_59

Toto_60

Toto_61

Toto_62

Toto_63

Toto_64

Toto_65

Toto_66

Toto_67
(Erf)

Toto_68

Toto_69

Toto_70

Toto_71

Toto_72

Toto_73

Toto_74

Toto_75

Toto_76

Toto_77

Toto_78

Toto_79
(DnaB)

Toto_80

Toto_81

Toto_82

Toto_83

Toto_84

Toto_85

Toto_86
(polynucleotide kinase)

Toto_87

Toto_88

Toto_89
(RNA ligase)

Toto_90

Toto_91
(WhiB family transcription factor)

Toto_92

Toto_93

Toto_94

Toto_95

Toto_96

Toto_97
(methyltransferase)

Toto_98
(single-stranded DNA binding protein)

Toto_99
(ClpP-like protease)

Toto_100

Toto_101

Toto_102

Toto_103

Toto_104

Toto_107

Toto_108

Toto_109

Toto_110

Toto_111

Toto_112
(DnaQ-like protein)

Toto_113

Toto_114
(RecA)

Toto_115

Toto_116

Toto_117

Toto_118

Toto_119

Toto_120

Toto_121

Toto_122

Toto_123

Toto_124

Toto_125

Toto_126

Toto_127
(ParB-like nuclease domain)

Toto_128

Toto_129

Toto_130

Toto_131

Toto_132

Toto_133

Toto_134

Toto_135

Toto_136

Toto_137

Toto_138

Toto_139

Toto_140

Toto_141

Toto_142

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionJN006061
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
SEA Designator 2008HHMITotoKWB
SEA Lysate Titer 3.7e9
Pitt Freezer Box# 3
Pitt Freezer Box Grid# B9
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload