Mycobacterium phage HufflyPuff
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Detailed Information for Phage HufflyPuff
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByPrajapati K, Vu C, Wadhwa N, Wood B
Year Found2011
Location FoundLos Angeles, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramUnavailable
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates34.0695 N, 118.4387 W Map
Discovery NotesPhage isolation performed by Krishna Prajapati, Christine Vu, Nisha Wadhwa, and Brandi Wood (spring 2011). DNA preparation performed by Krisanavane Reddi. Assembly performed by William Villella (UCLA) and confirmed by Charles Bowman (University of Pittsburgh). Preliminary genome annotation performed by Gregory Asatrian, Kevork Khadarian, Cherise Meyerson, and Laya Reddy (winter 2012). Final annotation analysis performed by Debbie Jacobs-Sera in summer 2013 (University of Pittsburgh).
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 5, 2013
Sequencing FacilityThe UCLA Genotyping and Sequencing Core
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage320
Genome length (bp)76323
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content63.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Other Cluster Members
Plaque NotesPlaque morphology: heterogeneous mix of large, turbid plaques, with smaller, clear plaques

Average plaque size: 5-7 mm diameter
MorphotypeSiphoviridae
Final annotation complete?Yes
Number of Genes146
Number of tRNAs2
Has been Phamerated?Yes
Gene List

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HufflyPuff_1

HufflyPuff_2

HufflyPuff_3

HufflyPuff_4
(Terminase, small subunit)

HufflyPuff_5

HufflyPuff_6

HufflyPuff_7
(Terminase, large subunit)

HufflyPuff_8

HufflyPuff_9
(Portal)

HufflyPuff_10
(Capsid maturation protease)

HufflyPuff_11
(Major Capsid)

HufflyPuff_12

HufflyPuff_13

HufflyPuff_14

HufflyPuff_15

HufflyPuff_16

HufflyPuff_17
(Major Tail)

HufflyPuff_18
(Tail Assembly Chaperone)

HufflyPuff_19
(Tail Assembly Chaperone)

HufflyPuff_20
(HNH endonuclease)

HufflyPuff_21
(Tapemeasure)

HufflyPuff_22
(Minor Tail)

HufflyPuff_23
(Minor Tail)

HufflyPuff_24
(Minor Tail)

HufflyPuff_25
(Minor Tail)

HufflyPuff_26
(D-ala-D-ala carboxypeptidase)

HufflyPuff_27

HufflyPuff_28

HufflyPuff_29

HufflyPuff_30

HufflyPuff_31
(LysA)

HufflyPuff_32
(Holin)

HufflyPuff_33

HufflyPuff_34
(LysB)

HufflyPuff_35

HufflyPuff_36
(NrdH)

HufflyPuff_37
(lsr2-like)

HufflyPuff_38

HufflyPuff_39

HufflyPuff_40

HufflyPuff_41

HufflyPuff_42

HufflyPuff_43

HufflyPuff_44

HufflyPuff_45

HufflyPuff_46

HufflyPuff_47

HufflyPuff_48

HufflyPuff_49

HufflyPuff_50

HufflyPuff_51

HufflyPuff_52

HufflyPuff_53

HufflyPuff_54
(Integrase (Y-Int))

HufflyPuff_55

HufflyPuff_56

HufflyPuff_57

HufflyPuff_58

HufflyPuff_59

HufflyPuff_60
(Pentapeptide repeat)

HufflyPuff_61

HufflyPuff_62

HufflyPuff_63

HufflyPuff_64

HufflyPuff_65

HufflyPuff_66

HufflyPuff_67

HufflyPuff_68

HufflyPuff_69

HufflyPuff_70

HufflyPuff_71

HufflyPuff_72
(Erf)

HufflyPuff_73

HufflyPuff_74

HufflyPuff_75

HufflyPuff_76

HufflyPuff_77

HufflyPuff_78

HufflyPuff_79

HufflyPuff_80
(HNH domain)

HufflyPuff_81

HufflyPuff_82

HufflyPuff_83

HufflyPuff_84
(DnaB-like helicase)

HufflyPuff_85

HufflyPuff_86

HufflyPuff_87

HufflyPuff_88

HufflyPuff_89

HufflyPuff_90

HufflyPuff_91
(Pnk)

HufflyPuff_92

HufflyPuff_93
(RNA ligase)

HufflyPuff_94

HufflyPuff_95
(WhiB)

HufflyPuff_96

HufflyPuff_97

HufflyPuff_98

HufflyPuff_99

HufflyPuff_100

HufflyPuff_101
(DNA methylase)

HufflyPuff_102
(ssDNA binding)

HufflyPuff_103
(Clp protease)

HufflyPuff_104

HufflyPuff_105

HufflyPuff_106

HufflyPuff_107

HufflyPuff_108

HufflyPuff_111

HufflyPuff_112

HufflyPuff_113

HufflyPuff_114

HufflyPuff_115
(DnaQ-like)

HufflyPuff_116

HufflyPuff_117
(RecA)

HufflyPuff_118

HufflyPuff_119

HufflyPuff_120

HufflyPuff_121

HufflyPuff_122

HufflyPuff_123

HufflyPuff_124

HufflyPuff_125

HufflyPuff_126

HufflyPuff_127

HufflyPuff_128

HufflyPuff_129

HufflyPuff_130

HufflyPuff_131

HufflyPuff_132

HufflyPuff_133

HufflyPuff_134

HufflyPuff_135

HufflyPuff_136

HufflyPuff_137

HufflyPuff_138

HufflyPuff_139

HufflyPuff_140

HufflyPuff_141

HufflyPuff_142

HufflyPuff_143

HufflyPuff_144

HufflyPuff_145

HufflyPuff_146

HufflyPuff_147

HufflyPuff_148
(HNH domain)

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKF562100
Refseq NumberNC_022981
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload