Mycobacterium phage MadamMonkfish
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Detailed Information for Phage MadamMonkfish
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByNatascha Richardson
Year Found2011
Location FoundRavenna, KY USA
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates37.689 N, 83.946 W Map
Discovery NotesSoil was wet due to recent rainfall. Soil was coarse and contained weed fragments. Sample was taken from a hill about 100 yards from Cow Creek Road in Ravenna, Kentucky. Sample was taken at 11:35 A.M. on September 5th, 2011. Soil was taken from 1-3 inches below the surface. The temperature outside was 19.4 degrees Celsius.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 2, 2012
Sequencing FacilityVirginia Commonwealth University Nucleic Acids Research Facilities
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage34
Genome length (bp)75554
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content63.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Other Cluster Members
Plaque NotesPlaques are small and round with clear centers and a small turbid clearing zone around the plaque.
MorphotypeSiphoviridae
Final annotation complete?No
Has been Phamerated?Yes
Gene List

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MadamMonkfish_1

MadamMonkfish_2

MadamMonkfish_3

MadamMonkfish_4

MadamMonkfish_5
(terminase small subunit)

MadamMonkfish_6

MadamMonkfish_7

MadamMonkfish_8
(terminase large subunit)

MadamMonkfish_9

MadamMonkfish_10
(portal protein)

MadamMonkfish_11
(capsid maturation protease)

MadamMonkfish_12
(major capsid protein)

MadamMonkfish_13
(head-to-tail connector protein)

MadamMonkfish_14
(head-to-tail connector protein)

MadamMonkfish_15
(head-to-tail connector protein)

MadamMonkfish_16
(head-to-tail connector protein)

MadamMonkfish_17
(head-to-tail connector protein)

MadamMonkfish_18
(major tail protein)

MadamMonkfish_19

MadamMonkfish_20
(tail assembly chaperone)

MadamMonkfish_21
(tail assembly chaperone)

MadamMonkfish_22
(tape measure protein)

MadamMonkfish_23
(minor tail protein)

MadamMonkfish_24
(minor tail protein)

MadamMonkfish_25
(minor tail protein)

MadamMonkfish_26
(minor tail protein)

MadamMonkfish_27
(minor tail protein)

MadamMonkfish_28
(minor tail protein)

MadamMonkfish_29

MadamMonkfish_30
(helix-turn-helix DNA binding domain protein)

MadamMonkfish_31
(membrane protein)

MadamMonkfish_32
(lysin A)

MadamMonkfish_33
(holin)

MadamMonkfish_34

MadamMonkfish_35
(lysin B)

MadamMonkfish_36
(membrane protein)

MadamMonkfish_37
(glutaredoxin)

MadamMonkfish_38

MadamMonkfish_39
(DNA bridging protein)

MadamMonkfish_40

MadamMonkfish_41

MadamMonkfish_42

MadamMonkfish_43

MadamMonkfish_44

MadamMonkfish_45

MadamMonkfish_46

MadamMonkfish_47

MadamMonkfish_48

MadamMonkfish_49

MadamMonkfish_50

MadamMonkfish_51
(membrane protein)

MadamMonkfish_52
(integrase)

MadamMonkfish_53

MadamMonkfish_54
(membrane protein)

MadamMonkfish_55
(immunity repressor)

MadamMonkfish_56

MadamMonkfish_57
(membrane protein)

MadamMonkfish_58
(pentapeptide repeat protein)

MadamMonkfish_59

MadamMonkfish_60

MadamMonkfish_61

MadamMonkfish_62

MadamMonkfish_63

MadamMonkfish_64

MadamMonkfish_65

MadamMonkfish_66

MadamMonkfish_67

MadamMonkfish_68

MadamMonkfish_69
(ERF family ssDNA binding protein)

MadamMonkfish_70

MadamMonkfish_71

MadamMonkfish_72

MadamMonkfish_73

MadamMonkfish_74
(membrane protein)

MadamMonkfish_75

MadamMonkfish_76
(ssDNA binding protein)

MadamMonkfish_77
(HNH endonuclease)

MadamMonkfish_78

MadamMonkfish_79

MadamMonkfish_80

MadamMonkfish_81
(dsDNA helicase)

MadamMonkfish_82

MadamMonkfish_83

MadamMonkfish_84

MadamMonkfish_85

MadamMonkfish_86
(resolvase)

MadamMonkfish_87
(membrane protein)

MadamMonkfish_88
(polynucleotide kinase)

MadamMonkfish_89

MadamMonkfish_90

MadamMonkfish_91
(RNA ligase)

MadamMonkfish_92
(helix-turn-helix DNA binding domain protein)

MadamMonkfish_93
(WhiB family transcription factor)

MadamMonkfish_94

MadamMonkfish_95

MadamMonkfish_96

MadamMonkfish_97

MadamMonkfish_98

MadamMonkfish_99
(DNA methylase)

MadamMonkfish_100
(ssDNA binding protein)

MadamMonkfish_101
(protease)

MadamMonkfish_102

MadamMonkfish_103

MadamMonkfish_104
(helix-turn-helix DNA binding domain protein)

MadamMonkfish_105
(HNH endonuclease)

MadamMonkfish_108

MadamMonkfish_109

MadamMonkfish_110
(glycosyltransferase)

MadamMonkfish_111

MadamMonkfish_112
(DNA polymerase III subunit)

MadamMonkfish_113

MadamMonkfish_114
(DNA recombinase)

MadamMonkfish_115

MadamMonkfish_116

MadamMonkfish_117

MadamMonkfish_118

MadamMonkfish_119

MadamMonkfish_120

MadamMonkfish_121

MadamMonkfish_122

MadamMonkfish_123

MadamMonkfish_124

MadamMonkfish_125

MadamMonkfish_126

MadamMonkfish_127

MadamMonkfish_128
(helix-turn-helix DNA binding domain protein)

MadamMonkfish_129
(dsDNA partitioning protein)

MadamMonkfish_130

MadamMonkfish_131

MadamMonkfish_132

MadamMonkfish_133

MadamMonkfish_134

MadamMonkfish_135

MadamMonkfish_136

MadamMonkfish_137

MadamMonkfish_138

MadamMonkfish_139

MadamMonkfish_140

MadamMonkfish_141

MadamMonkfish_142

MadamMonkfish_143

MadamMonkfish_144

MadamMonkfish_145

MadamMonkfish_146
(peptidase)

MadamMonkfish_147

MadamMonkfish_148
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF668277
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload