Mycobacterium phage NoSleep
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Detailed Information for Phage NoSleep
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByPaul Turner
Year Found2012
Location FoundCanton, MO United States
Finding InstitutionCulver-Stockton College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates40.12983 N, 91.528673 W Map
Discovery NotesI got my sample of dirt to start this experiment from a slightly wet patch of dirt underneath a bridge on the campus of Culver-Stockton College.
Naming NotesI'm not very creative so this is the first thing I thought of.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 5, 2013
Sequencing FacilityVirginia Commonwealth University Nucleic Acids Research Facilities
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage130
Sanger Finishing Reactions11
Genome length (bp)74655
Character of genome ends3' Sticky Overhang
Overhang Length9 bp
Overhang SequenceCGCTTGTCA
GC Content63.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Other Cluster Members
Plaque NotesClear, on average 205.5 nm
MorphotypeSiphoviridae
Final annotation complete?No
Has been Phamerated?Yes
Gene List

Click to View

NoSleep_1

NoSleep_2

NoSleep_3

NoSleep_4

NoSleep_5
(terminase, small subunit)

NoSleep_6

NoSleep_7

NoSleep_8

NoSleep_9
(terminase, large subunit)

NoSleep_10

NoSleep_11
(portal)

NoSleep_12
(capsid maturation protease)

NoSleep_13
(major capsid protein)

NoSleep_14

NoSleep_15

NoSleep_16

NoSleep_17

NoSleep_18

NoSleep_19
(major tail subunit)

NoSleep_20

NoSleep_21
(tail assembly chaperone)

NoSleep_22
(tail assembly chaperone)

NoSleep_23
(tapemeasure)

NoSleep_24
(minor tail protein)

NoSleep_25
(minor tail protein)

NoSleep_26
(minor tail protein)

NoSleep_27
(minor tail protein)

NoSleep_28
(D-ala-D-ala carboxypeptidase)

NoSleep_29

NoSleep_30

NoSleep_31

NoSleep_32

NoSleep_33
(lysin A)

NoSleep_34
(holin)

NoSleep_35

NoSleep_36
(lysin B)

NoSleep_37

NoSleep_38
(NrdH-like)

NoSleep_39
(Lsr2)

NoSleep_40

NoSleep_41

NoSleep_42

NoSleep_43

NoSleep_44

NoSleep_45

NoSleep_46

NoSleep_47

NoSleep_48

NoSleep_49

NoSleep_50

NoSleep_51

NoSleep_52

NoSleep_53

NoSleep_54
(integrase, Y-int)

NoSleep_55

NoSleep_56

NoSleep_57
(immunity repressor)

NoSleep_58

NoSleep_59

NoSleep_60
(pentapeptide repeat family)

NoSleep_61

NoSleep_62

NoSleep_63

NoSleep_64

NoSleep_65

NoSleep_66

NoSleep_67

NoSleep_68

NoSleep_69

NoSleep_70
(Erf)

NoSleep_71

NoSleep_72

NoSleep_73

NoSleep_74

NoSleep_75

NoSleep_76

NoSleep_77

NoSleep_78
(HNH endonuclease domain)

NoSleep_79

NoSleep_80

NoSleep_81

NoSleep_82
(DnaB-like helicase)

NoSleep_83

NoSleep_84

NoSleep_85

NoSleep_86

NoSleep_87
(DNA binding, putative HJC)

NoSleep_88

NoSleep_89
(polynucleotide kinase)

NoSleep_90

NoSleep_91

NoSleep_92
(RNA ligase)

NoSleep_93
(HTH DNA binding domain)

NoSleep_94
(WhiB)

NoSleep_95

NoSleep_96

NoSleep_97

NoSleep_98

NoSleep_99

NoSleep_100
(DNA methylase)

NoSleep_101
(ssDNA binding protein)

NoSleep_102
(Clp protease)

NoSleep_103

NoSleep_104

NoSleep_105
(HTH DNA binding protein)

NoSleep_106
(HNH endonuclease)

NoSleep_109

NoSleep_110

NoSleep_111
(CMP N-glycosidase-like)

NoSleep_112

NoSleep_113
(DnaQ-like exonuclease)

NoSleep_114

NoSleep_115
(RecA)

NoSleep_116

NoSleep_117

NoSleep_118

NoSleep_119

NoSleep_120

NoSleep_121

NoSleep_122

NoSleep_123

NoSleep_124

NoSleep_125

NoSleep_126

NoSleep_127

NoSleep_128
(ParBc domain)

NoSleep_129

NoSleep_130

NoSleep_131

NoSleep_132

NoSleep_133

NoSleep_134

NoSleep_135

NoSleep_136

NoSleep_137

NoSleep_138

NoSleep_139

NoSleep_140

NoSleep_141

NoSleep_142

NoSleep_143

NoSleep_144

NoSleep_145
(HNH endonuclease domain)

Submitted Annotation in DNA Master FormatDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKT020852
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload