Mycobacterium phage MISSy
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Detailed Information for Phage MISSy
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByTeam Gone Viral
Year Found2014
Location FoundLos Angeles, CA United States
Finding InstitutionUniversity of California, Los Angeles
ProgramUnavailable
From enriched soil sample?Yes
Isolation Temperature35°C
GPS Coordinates34.055939 N, 118.4424 W Map
Discovery NotesPhage isolation performed in Spring 2014 and genome annotation performed in Fall 2014 by Team Gone Viral: Michelle Liao, Iger Ostreni, Sara Komenan, Shayli Swaim. Annotation quality checked by Edric Yoon, Lei Yeh, Iger Ostreni and Emily Xu. DNA preparation performed by Dr. Krisanavane Reddi (UCLA). Assembly performed by Dr. Jordan Moberg Parker (UCLA).

Sample description: Soil from planter in an urban area with low foot traffic outside of Roberts Luxury Apartments on April 2, 2014.
Depth from which sample was taken (in inches or cm): 0-3cm
Air temperature if sample collected outdoors (in degrees Celsius): 14oC
Additional information of interest or note: There was 0.25 inches of precipitation the night before.
Naming NotesThis name was picked from the first letters of the names of each person on the team: Michelle, Iger, Sara, and Shayli.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 23, 2015
Sequencing FacilityThe UCLA Genotyping and Sequencing Core
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage63
Genome length (bp)75808
Character of genome ends3' Sticky Overhang
Overhang Length9 bp
Overhang SequenceCGCTTGTCA
GC Content63.1%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Other Cluster Members
Plaque NotesBulls-eye morphology with clear inner circle, turbid middle ring, clear outer ring. Average diameter of ~4.5 ± 0.58 mm. Size range of 3.5-6.0mm.
MorphotypeSiphoviridae
Final annotation complete?No
Number of Genes127
Number of tRNAs2
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload