Mycobacterium phage MISSy
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Detailed Information for Phage MISSy
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByTeam Gone Viral
Year Found2014
Location FoundLos Angeles, CA United States
Finding InstitutionUniversity of California, Los Angeles
ProgramUnavailable
From enriched soil sample?Yes
Isolation Temperature35°C
GPS Coordinates34.055939 N, 118.4424 W Map
Discovery NotesPhage isolation performed in Spring 2014 and genome annotation performed in Fall 2014 by Team Gone Viral: Michelle Liao, Iger Ostreni, Sara Komenan, Shayli Swaim. Annotation quality checked by Edric Yoon, Lei Yeh, Iger Ostreni and Emily Xu. DNA preparation performed by Dr. Krisanavane Reddi (UCLA). Assembly performed by Dr. Jordan Moberg Parker (UCLA).

Sample description: Soil from planter in an urban area with low foot traffic outside of Roberts Luxury Apartments on April 2, 2014.
Depth from which sample was taken (in inches or cm): 0-3cm
Air temperature if sample collected outdoors (in degrees Celsius): 14oC
Additional information of interest or note: There was 0.25 inches of precipitation the night before.
Naming NotesThis name was picked from the first letters of the names of each person on the team: Michelle, Iger, Sara, and Shayli.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 23, 2015
Sequencing FacilityThe UCLA Genotyping and Sequencing Core
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage63
Genome length (bp)75808
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content63.1%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Other Cluster Members
Plaque NotesBulls-eye morphology with clear inner circle, turbid middle ring, clear outer ring. Average diameter of ~4.5 ± 0.58 mm. Size range of 3.5-6.0mm.
MorphotypeSiphoviridae
Final annotation complete?No
Number of Genes127
Number of tRNAs2
Has been Phamerated?Yes
Gene List

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MISSy_1

MISSy_2

MISSy_3

MISSy_4

MISSy_5
(terminase small subunit)

MISSy_6

MISSy_7

MISSy_8
(terminase large subunit)

MISSy_9

MISSy_10
(portal protein)

MISSy_11
(capsid maturation protease)

MISSy_12
(major capsid protein)

MISSy_13
(head-to-tail connector protein)

MISSy_14
(head-to-tail connector protein)

MISSy_15
(head-to-tail connector protein)

MISSy_16
(head-to-tail connector protein)

MISSy_17
(head-to-tail connector protein)

MISSy_18
(major tail protein)

MISSy_19
(tail assembly chaperone)

MISSy_20
(tail assembly chaperone)

MISSy_21
(tapemeasure protein)

MISSy_22
(minor tail protein)

MISSy_23
(minor tail protein)

MISSy_24
(minor tail protein)

MISSy_25
(minor tail protein)

MISSy_26
(minor tail protein)

MISSy_27
(minor tail protein)

MISSy_28

MISSy_29
(helix-turn-helix DNA binding domain protein)

MISSy_30

MISSy_31
(lysin A)

MISSy_32
(holin)

MISSy_33

MISSy_34
(lysin B)

MISSy_35

MISSy_36
(glutaredoxin)

MISSy_37
(DNA bridging protein)

MISSy_38

MISSy_39

MISSy_40

MISSy_41

MISSy_42

MISSy_43

MISSy_44

MISSy_45

MISSy_46

MISSy_47

MISSy_48

MISSy_49

MISSy_50

MISSy_51
(membrane domain protein)

MISSy_52
(integrase)

MISSy_53

MISSy_54

MISSy_55
(immunity repressor)

MISSy_56

MISSy_57

MISSy_58
(pentapeptide repeat protein)

MISSy_59

MISSy_60

MISSy_61

MISSy_62

MISSy_63

MISSy_64

MISSy_65

MISSy_66

MISSy_67

MISSy_68
(exonuclease)

MISSy_69

MISSy_70
(ERF family ssDNA binding protein)

MISSy_71

MISSy_72

MISSy_73

MISSy_74

MISSy_75

MISSy_76

MISSy_77

MISSy_78
(HNH endonuclease)

MISSy_79

MISSy_80

MISSy_81

MISSy_82
(dsDNA helicase)

MISSy_83

MISSy_84
(WhiB family transcription factor)

MISSy_85

MISSy_86

MISSy_87
(holliday junction resolvase)

MISSy_88

MISSy_89
(polynucleotide kinase)

MISSy_90

MISSy_91

MISSy_92
(RNA ligase)

MISSy_93
(helix-turn-helix DNA binding domain protein)

MISSy_94
(WhiB family transcription factor)

MISSy_95

MISSy_96

MISSy_97

MISSy_98

MISSy_99
(helix-turn-helix DNA binding domain protein)

MISSy_100
(DNA methylase)

MISSy_101
(ssDNA binding protein)

MISSy_102
(protease)

MISSy_103

MISSy_104

MISSy_105

MISSy_106
(helix-turn-helix DNA binding protein)

MISSy_107
(HNH endonuclease)

MISSy_110

MISSy_111

MISSy_112

MISSy_113

MISSy_114
(DNA polymerase III subunit)

MISSy_115

MISSy_116
(DNA recombinase)

MISSy_117

MISSy_118

MISSy_119

MISSy_120

MISSy_121

MISSy_122

MISSy_123

MISSy_124

MISSy_125

MISSy_126

MISSy_127

MISSy_128

MISSy_129
(helix-turn-helix DNA binding domain protein)

MISSy_130

MISSy_131

MISSy_132

MISSy_133

MISSy_134

MISSy_135

MISSy_136

MISSy_137

MISSy_138

MISSy_139

MISSy_140

MISSy_141

MISSy_142

MISSy_143

MISSy_144

MISSy_145

MISSy_146

MISSy_147
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF919524
Refseq NumberNone yet
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload