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Mycobacterium phage Ukulele
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Detailed Information for Phage Ukulele
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByMaryanne LaFollette and Matthew Sheltra
Year Found2011
Location FoundOld Orchard Beach, ME USA
Finding InstitutionUniversity of Maine, Honors College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS CoordinatesUnavailable
Discovery NotesSoil sample was found in a flower garden ~2 cm deep. Temperature was approximately 69?, and soil was slightly damp.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 31, 2012
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIon Torrent Sequencing
Approximate Shotgun Coverage133
Genome length (bp)75114
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content63.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Other Cluster Members
Plaque NotesPlaque morphology ranges from ~0.5 mm to ~2.0 mm, and is slightly turbid.
MorphotypeSiphoviridae
Final annotation complete?No
Has been Phamerated?Yes
Gene List

Click to View

Ukulele_1

Ukulele_2

Ukulele_3

Ukulele_4

Ukulele_5
(Terminase, small subunit)

Ukulele_6

Ukulele_7

Ukulele_8
(Terminase, large subunit)

Ukulele_9

Ukulele_10
(Portal protein)

Ukulele_11

Ukulele_12
(Major Capsid protein)

Ukulele_13
(Head-to-tail connector protein)

Ukulele_14
(Head-to-tail connector protein)

Ukulele_15
(Head-to-tail connector protein)

Ukulele_16
(Head-to-tail connector protein)

Ukulele_17
(Head-to-tail connector protein)

Ukulele_18
(Major tail protein)

Ukulele_19

Ukulele_20
(Tail assembly chaperone)

Ukulele_21
(Tail assembly chaperone)

Ukulele_22
(Tape meaure protein)

Ukulele_23
(Minor tail protein)

Ukulele_24
(Minor tail protein)

Ukulele_25
(Minor tail protein)

Ukulele_26
(Minor tail protein)

Ukulele_27
(Minor tail protein, D-ala-D-ala carboxypeptidase)

Ukulele_28

Ukulele_29

Ukulele_30

Ukulele_31

Ukulele_32
(Lysin A)

Ukulele_33
(Holin)

Ukulele_34

Ukulele_35
(Lysin B)

Ukulele_36

Ukulele_37
(NrdH glutaredoxin)

Ukulele_38

Ukulele_39
(Lsr2 DNA bridging protein)

Ukulele_40

Ukulele_41

Ukulele_42

Ukulele_43

Ukulele_44

Ukulele_45

Ukulele_46

Ukulele_47

Ukulele_48

Ukulele_49
(Integrase (Y-Int))

Ukulele_50

Ukulele_51

Ukulele_52
(Immunity Repressor)

Ukulele_53

Ukulele_54

Ukulele_55
(Pentapeptide repeat protein)

Ukulele_56

Ukulele_57

Ukulele_58

Ukulele_59

Ukulele_60

Ukulele_61

Ukulele_62

Ukulele_63

Ukulele_64
(ssDNA binding protein, ERF family)

Ukulele_65

Ukulele_66

Ukulele_67

Ukulele_68

Ukulele_69

Ukulele_70

Ukulele_71

Ukulele_72
(HNH endonuclease)

Ukulele_73

Ukulele_74

Ukulele_75

Ukulele_76
(DnaB dsDNA helicase)

Ukulele_77

Ukulele_78

Ukulele_79

Ukulele_80

Ukulele_81

Ukulele_82

Ukulele_83
(polynucleotide kinase)

Ukulele_84

Ukulele_85

Ukulele_86
(RNA Ligase)

Ukulele_87

Ukulele_88
(WhiB family transcription factor)

Ukulele_89

Ukulele_90

Ukulele_91

Ukulele_92

Ukulele_93

Ukulele_94
(DNA methylase)

Ukulele_95

Ukulele_96
(ClpP protease)

Ukulele_97

Ukulele_98

Ukulele_99

Ukulele_100
(HNH endonuclease)

Ukulele_103

Ukulele_104

Ukulele_105
(hydrolase)

Ukulele_106

Ukulele_107

Ukulele_108
(DnaQ exonuclease (DNA polymerase III subunit))

Ukulele_109

Ukulele_110
(RecA DNA Recombinase)

Ukulele_111

Ukulele_112

Ukulele_113

Ukulele_114

Ukulele_115

Ukulele_116

Ukulele_117

Ukulele_118

Ukulele_119

Ukulele_120

Ukulele_121

Ukulele_122

Ukulele_123

Ukulele_124

Ukulele_125

Ukulele_126

Ukulele_127

Ukulele_128

Ukulele_129

Ukulele_130

Ukulele_131

Ukulele_132

Ukulele_133

Ukulele_134

Ukulele_135

Ukulele_136

Ukulele_137

Ukulele_138

Ukulele_139

Ukulele_140

Ukulele_141

Ukulele_142

Ukulele_143
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKT373978
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload