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Mycobacterium phage Sassay
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Detailed Information for Phage Sassay
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByTeam M.C. Smegger
Year Found2014
Location FoundLos Angeles, CA United States
Finding InstitutionUniversity of California, Los Angeles
From enriched soil sample?Yes
Isolation Temperature35°C
GPS Coordinates34.07 N, 118.452 W Map
Discovery NotesPhage isolation performed in Spring 2014 and genome annotation performed in Fall 2014 by Team M.C. Smegger: Meghana Attaluri, Mark LaVigne, Saul Lincoln, Cydney Nichols. Annotation was quality checked by Edric Yoon and Lei Yeh. TA: Emma Goodwin. DNA preparation performed by Dr. Krisanavane Reddi (UCLA). Assembly performed by Dr. Jordan Moberg Parker (UCLA).

Sample description: Sample collected from moist soil from a flower bed by Cydney Nichols on April 4, 2014.
3 cm deep
Temperature: 15.56oC
Urban environment close to foot/vehicle traffic
Naming NotesPhage Sassay (combination of “sassy” and the commonly used lab term “assay”)
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 23, 2015
Sequencing FacilityThe UCLA Genotyping and Sequencing Core
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage126
Genome length (bp)73495
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content63.0%
Fasta file available?Yes: Download fasta file
Other Cluster Members
Plaque NotesTurbid, with halos occasionally, 2mm in radius
Final annotation complete?No
Number of Genes139
Number of tRNAs2
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload