Mycobacterium phage Sassay
Know something about this phage that we don't? Modify its data.
Detailed Information for Phage Sassay
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByTeam M.C. Smegger
Year Found2014
Location FoundLos Angeles, CA United States
Finding InstitutionUniversity of California, Los Angeles
ProgramUnavailable
From enriched soil sample?Yes
Isolation Temperature35°C
GPS Coordinates34.07 N, 118.452 W Map
Discovery NotesPhage isolation performed in Spring 2014 and genome annotation performed in Fall 2014 by Team M.C. Smegger: Meghana Attaluri, Mark LaVigne, Saul Lincoln, Cydney Nichols. Annotation was quality checked by Edric Yoon and Lei Yeh. TA: Emma Goodwin. DNA preparation performed by Dr. Krisanavane Reddi (UCLA). Assembly performed by Dr. Jordan Moberg Parker (UCLA).

Sample description: Sample collected from moist soil from a flower bed by Cydney Nichols on April 4, 2014.
3 cm deep
Temperature: 15.56oC
Urban environment close to foot/vehicle traffic
Naming NotesPhage Sassay (combination of “sassy” and the commonly used lab term “assay”)
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 23, 2015
Sequencing FacilityThe UCLA Genotyping and Sequencing Core
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage126
Genome length (bp)73495
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content63.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Other Cluster Members
Plaque NotesTurbid, with halos occasionally, 2mm in radius
MorphotypeSiphoviridae
Final annotation complete?No
Number of Genes139
Number of tRNAs2
Has been Phamerated?Yes
Gene List

Click to View

Sassay_1

Sassay_2

Sassay_3

Sassay_4

Sassay_5
(terminase small subunit)

Sassay_6

Sassay_7

Sassay_8
(terminase large subunit)

Sassay_9

Sassay_10
(portal protein)

Sassay_11
(capsid maturation protein)

Sassay_12
(major capsid protein)

Sassay_13
(head-to-tail connector protein)

Sassay_14
(head-to-tail connector protein)

Sassay_15
(head-to-tail connector protein)

Sassay_16
(head-to-tail connector protein)

Sassay_17
(head-to-tail connector protein)

Sassay_18
(major tail protein)

Sassay_19
(tail assembly chaperone)

Sassay_20
(tail assembly chaperone)

Sassay_21
(tapemeasure protein)

Sassay_22
(minor tail protein)

Sassay_23
(minor tail protein)

Sassay_24
(minor tail protein)

Sassay_25
(minor tail protein)

Sassay_26
(minor tail protein)

Sassay_27
(minor tail protein)

Sassay_28

Sassay_29
(helix-turn-helix DNA binding domain protein)

Sassay_30

Sassay_31
(lysin A)

Sassay_32
(holin)

Sassay_33

Sassay_34
(lysin B)

Sassay_35

Sassay_36
(glutaredoxin)

Sassay_37
(DNA bridging protein)

Sassay_38

Sassay_39

Sassay_40

Sassay_41

Sassay_42

Sassay_43

Sassay_44

Sassay_45
(membrane domain protein)

Sassay_46
(integrase)

Sassay_47

Sassay_48

Sassay_49
(immunity repressor)

Sassay_50

Sassay_51

Sassay_52
(pentapeptide repeat protein)

Sassay_53

Sassay_54

Sassay_55

Sassay_56

Sassay_57

Sassay_58

Sassay_59

Sassay_60

Sassay_61

Sassay_62

Sassay_63

Sassay_64
(ERF family ssDNA binding protein)

Sassay_65

Sassay_66

Sassay_67

Sassay_68

Sassay_69

Sassay_70

Sassay_71

Sassay_72
(HNH endonuclease)

Sassay_73

Sassay_74

Sassay_75

Sassay_76
(dsDNA helicase)

Sassay_77

Sassay_78
(WhiB family transcription factor)

Sassay_79

Sassay_80

Sassay_81
(holliday junction resolvase)

Sassay_82

Sassay_83
(polynucleotide kinase)

Sassay_84

Sassay_85

Sassay_86
(RNA ligase)

Sassay_87
(helix-turn-helix DNA binding domain protein)

Sassay_88
(WhiB family transcription factor)

Sassay_89

Sassay_90

Sassay_91

Sassay_92

Sassay_93

Sassay_94
(DNA methylase)

Sassay_95
(ssDNA binding protein)

Sassay_96
(protease)

Sassay_97

Sassay_98

Sassay_99

Sassay_100
(helix-turn-helix DNA binding domain protein)

Sassay_101
(HNH endonuclease)

Sassay_104

Sassay_105

Sassay_106

Sassay_107

Sassay_108
(DNA polymerase III subunit)

Sassay_109

Sassay_110
(DNA recombinase)

Sassay_111

Sassay_112

Sassay_113

Sassay_114

Sassay_115

Sassay_116

Sassay_117

Sassay_118

Sassay_119

Sassay_120

Sassay_121

Sassay_122

Sassay_123
(helix-turn-helix DNA binding domain protein)

Sassay_124

Sassay_125

Sassay_126

Sassay_127

Sassay_128

Sassay_129

Sassay_130

Sassay_131

Sassay_132

Sassay_133

Sassay_134

Sassay_135

Sassay_136

Sassay_137

Sassay_138

Sassay_139

Sassay_140

Sassay_141

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF919529
Refseq NumberNone yet
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload