Mycobacterium phage DoctorDiddles
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Detailed Information for Phage DoctorDiddles
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByConnor Patterson
Year Found2017
Location FoundBowling Green, KY United States
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation Temperature27°C
GPS Coordinates37.0021 N, 86.4188 W Map
Discovery NotesThe phage was discovered in the soil on the shore of a creek, it was the soil at the surface. It was a very warm day when discovered, 80 degrees Fahrenheit with minimal clouds at 12:18 PM.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedApr 13, 2018
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage200
Genome length (bp)75070
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content62.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusBeing Annotated (Expected completion by 10/13/2018)
Plaque NotesThe plaques that formed from this phage were bulls eye shaped. The plaques were non-turbid and very clear in the middle of the plaque but the edges of the plaque were more turbid and unclear.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

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Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.77 * 10^11 pfu
Pitt Freezer Box# 51
Pitt Freezer Box Grid# G5
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload