Mycobacterium phage DoctorDiddles
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Detailed Information for Phage DoctorDiddles
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByConnor Patterson
Year Found2017
Location FoundBowling Green, KY United States
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation Temperature27°C
GPS Coordinates37.0021 N, 86.4188 W Map
Discovery NotesThe phage was discovered in the soil on the shore of a creek, it was the soil at the surface. It was a very warm day when discovered, 80 degrees Fahrenheit with minimal clouds at 12:18 PM.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedApr 13, 2018
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage200
Genome length (bp)75070
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content62.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesThe plaques that formed from this phage were bulls eye shaped. The plaques were non-turbid and very clear in the middle of the plaque but the edges of the plaque were more turbid and unclear.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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DoctorDiddles_1

DoctorDiddles_2

DoctorDiddles_3

DoctorDiddles_4

DoctorDiddles_5
(terminase, small subunit)

DoctorDiddles_6
(membrane protein)

DoctorDiddles_7

DoctorDiddles_8

DoctorDiddles_9
(terminase, large subunit)

DoctorDiddles_10

DoctorDiddles_11
(portal protein)

DoctorDiddles_12
(capsid maturation protease)

DoctorDiddles_13
(major capsid protein)

DoctorDiddles_14
(head-to-tail connector complex protein)

DoctorDiddles_15
(head-to-tail connector complex protein)

DoctorDiddles_16
(head-to-tail connector complex protein)

DoctorDiddles_17
(head-to-tail connector complex protein)

DoctorDiddles_18
(head-to-tail connector complex protein)

DoctorDiddles_19
(major tail protein)

DoctorDiddles_20
(tail assembly chaperone)

DoctorDiddles_21
(tail assembly chaperone)

DoctorDiddles_22
(tape measure protein)

DoctorDiddles_23
(minor tail protein)

DoctorDiddles_24
(minor tail protein)

DoctorDiddles_25
(minor tail protein)

DoctorDiddles_26
(minor tail protein)

DoctorDiddles_27
(minor tail protein, D-ala-D-ala carboxypeptidase)

DoctorDiddles_28
(minor tail protein)

DoctorDiddles_29

DoctorDiddles_30
(helix-turn-helix DNA binding domain)

DoctorDiddles_31
(membrane protein)

DoctorDiddles_32
(lysin A)

DoctorDiddles_33
(holin)

DoctorDiddles_34

DoctorDiddles_35
(lysin B)

DoctorDiddles_36
(membrane protein)

DoctorDiddles_37
(NrdH-like glutaredoxin)

DoctorDiddles_38

DoctorDiddles_39
(Lsr2-like DNA bridging protein)

DoctorDiddles_40

DoctorDiddles_41

DoctorDiddles_42

DoctorDiddles_43
(HNH endonuclease)

DoctorDiddles_44

DoctorDiddles_45

DoctorDiddles_46

DoctorDiddles_47

DoctorDiddles_48

DoctorDiddles_49

DoctorDiddles_50

DoctorDiddles_51
(membrane protein)

DoctorDiddles_52
(integrase, (Y-int))

DoctorDiddles_53

DoctorDiddles_54
(membrane protein)

DoctorDiddles_55
(immunity repressor)

DoctorDiddles_56

DoctorDiddles_57
(membrane protein)

DoctorDiddles_58
(pentapeptide repeat protein)

DoctorDiddles_59

DoctorDiddles_60

DoctorDiddles_61

DoctorDiddles_62

DoctorDiddles_63

DoctorDiddles_64

DoctorDiddles_65

DoctorDiddles_66
(exonuclease)

DoctorDiddles_67

DoctorDiddles_68
(ssDNA binding protein, ERF family)

DoctorDiddles_69

DoctorDiddles_70

DoctorDiddles_71

DoctorDiddles_72

DoctorDiddles_73

DoctorDiddles_74

DoctorDiddles_75
(DprA-like DNA processing chain A)

DoctorDiddles_76
(HNH endonuclease)

DoctorDiddles_77

DoctorDiddles_78

DoctorDiddles_79

DoctorDiddles_80
(DNAB-like dsDNA helicase)

DoctorDiddles_81

DoctorDiddles_82
(WhiB family transcription factor)

DoctorDiddles_83

DoctorDiddles_84

DoctorDiddles_85
(Holliday junction resolvase)

DoctorDiddles_86
(membrane protein)

DoctorDiddles_87
(polynucleotide kinase)

DoctorDiddles_88

DoctorDiddles_89
(membrane protein)

DoctorDiddles_90
(RNA ligase)

DoctorDiddles_91
(helix-turn-helix DNA binding domain)

DoctorDiddles_92
(WhiB family transcription factor)

DoctorDiddles_93

DoctorDiddles_94

DoctorDiddles_95

DoctorDiddles_96

DoctorDiddles_97

DoctorDiddles_98
(endonuclease VII)

DoctorDiddles_99
(ssDNA binding protein)

DoctorDiddles_100
(ClpP-like protease)

DoctorDiddles_101

DoctorDiddles_102

DoctorDiddles_103

DoctorDiddles_104
(helix-turn-helix DNA binding domain)

DoctorDiddles_105
(HNH endonuclease)

DoctorDiddles_108

DoctorDiddles_109

DoctorDiddles_110

DoctorDiddles_111

DoctorDiddles_112
(DnaQ-like exonuclease (DNA polymerase III subunit))

DoctorDiddles_113

DoctorDiddles_114
(RecA-like DNA Recombinase)

DoctorDiddles_115

DoctorDiddles_116

DoctorDiddles_117

DoctorDiddles_118

DoctorDiddles_119

DoctorDiddles_120

DoctorDiddles_121

DoctorDiddles_122

DoctorDiddles_123

DoctorDiddles_124

DoctorDiddles_125

DoctorDiddles_126
(helix-turn-helix DNA binding domain)

DoctorDiddles_127
(ParB-like nuclease domain)

DoctorDiddles_128

DoctorDiddles_129

DoctorDiddles_130

DoctorDiddles_131

DoctorDiddles_132

DoctorDiddles_133

DoctorDiddles_134

DoctorDiddles_135

DoctorDiddles_136

DoctorDiddles_137

DoctorDiddles_138

DoctorDiddles_139

DoctorDiddles_140

DoctorDiddles_141

DoctorDiddles_142

DoctorDiddles_143

DoctorDiddles_144

DoctorDiddles_145
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH399774
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.77 * 10^11 pfu
Pitt Freezer Box# 51
Pitt Freezer Box Grid# G5
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload