Mycobacterium phage Murica
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Detailed Information for Phage Murica
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByTeam Miley Virus
Year Found2013
Location FoundLos Angeles, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramUnavailable
From enriched soil sample?Yes
Isolation Temperature35°C
GPS Coordinates34.07 N, 118.451 W Map
Discovery NotesPhage isolation performed in Fall 2013 and genome annotation performed in Spring 2014 by Team Miley Virus: Kola Awe, Dami Oshinuga, and Apryl Stafford. Annotation quality checked by Lei Yeh, Edric Yoon, and Iger Ostreni. TA: Samantha Capati. Instructor: Dr. Jordan Moberg Parker. DNA preparation performed by Krisanavane Reddi (UCLA). Assembly performed by William Villella (UCLA).

Moist soil collected next to a mossy tree, foot traffic and regular sprinklers on October 3, 2013.
Naming NotesSlang for America.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedApr 11, 2014
Sequencing FacilityThe UCLA Genotyping and Sequencing Core
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage81
Genome length (bp)77053
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content63.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Other Cluster Members
Plaque NotesMurica plaque assays contain both completely clear plaques in addition to plaques with clear centers and turbid edges. Average size (diameter in mm): 3.5mm. Range (in mm): 3mm-5mm
MorphotypeSiphoviridae
Final annotation complete?No
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload