Mycobacterium phage Murica
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Detailed Information for Phage Murica
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByTeam Miley Virus
Year Found2013
Location FoundLos Angeles, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramUnavailable
From enriched soil sample?Yes
Isolation Temperature35°C
GPS Coordinates34.07 N, 118.451 W Map
Discovery NotesPhage isolation performed in Fall 2013 and genome annotation performed in Spring 2014 by Team Miley Virus: Kola Awe, Dami Oshinuga, and Apryl Stafford. Annotation quality checked by Lei Yeh, Edric Yoon, and Iger Ostreni. TA: Samantha Capati. Instructor: Dr. Jordan Moberg Parker. DNA preparation performed by Krisanavane Reddi (UCLA). Assembly performed by William Villella (UCLA).

Moist soil collected next to a mossy tree, foot traffic and regular sprinklers on October 3, 2013.
Naming NotesSlang for America.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedApr 11, 2014
Sequencing FacilityThe UCLA Genotyping and Sequencing Core
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage81
Genome length (bp)77053
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content63.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Other Cluster Members
Plaque NotesMurica plaque assays contain both completely clear plaques in addition to plaques with clear centers and turbid edges. Average size (diameter in mm): 3.5mm. Range (in mm): 3mm-5mm
MorphotypeSiphoviridae
Final annotation complete?No
Has been Phamerated?Yes
Gene List

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Murica_1

Murica_2

Murica_3

Murica_4
(terminase, small subunit)

Murica_5

Murica_6

Murica_7
(terminase, large subunit)

Murica_8

Murica_9
(portal protein)

Murica_10
(capsid maturation protease)

Murica_11
(major capsid protein)

Murica_12
(head-to-tail connector protein)

Murica_13
(head-to-tail connector protein)

Murica_14
(head-to-tail connector protein)

Murica_15
(head-to-tail connector protein)

Murica_16
(head-to-tail connector protein)

Murica_17
(major tail protein)

Murica_18
(tail assembly chaperone)

Murica_19
(tail assembly chaperone)

Murica_20
(HNH endonuclease VII)

Murica_21
(tape measure protein)

Murica_22
(minor tail protein)

Murica_23
(minor tail protein)

Murica_24
(minor tail protein)

Murica_25
(minor tail protein)

Murica_26
(minor tail protein, D-ala-D-ala carboxypeptidase)

Murica_27
(minor tail protein)

Murica_28

Murica_29
(helix-turn-helix DNA binding domain)

Murica_30

Murica_31
(lysin A)

Murica_32
(holin)

Murica_33

Murica_34
(lysin B)

Murica_35

Murica_36
(NrdH glutaredoxin)

Murica_37
(Lsr2 DNA bridging protein)

Murica_38

Murica_39

Murica_40

Murica_41

Murica_42

Murica_43

Murica_44

Murica_45

Murica_46

Murica_47

Murica_48

Murica_49

Murica_50

Murica_51

Murica_52

Murica_53
(membrane domain protein)

Murica_54
(integrase, (Y-int))

Murica_55

Murica_56

Murica_57
(immunity repressor)

Murica_58

Murica_59

Murica_60
(pentapeptide repeat protein)

Murica_61

Murica_62

Murica_63

Murica_64

Murica_65

Murica_66

Murica_67

Murica_68

Murica_69

Murica_70

Murica_71

Murica_72
(ssDNA binding protein, ERF family)

Murica_73

Murica_74

Murica_75

Murica_76

Murica_77

Murica_78

Murica_79

Murica_80
(HNH endonuclease)

Murica_81

Murica_82

Murica_83

Murica_84
(DnaB dsDNA helicase)

Murica_85

Murica_86
(WhiB family transcription factor)

Murica_87

Murica_88

Murica_89
(holliday junction resolvase)

Murica_90

Murica_91
(polynucleotide kinase)

Murica_92

Murica_93

Murica_94
(RNA ligase)

Murica_95
(helix-turn-helix DNA binding protein)

Murica_96
(WhiB family transcription factor)

Murica_97

Murica_98

Murica_99

Murica_100

Murica_101

Murica_102
(DNA methylase)

Murica_103
(ssDNA binding protein)

Murica_104
(ClpP protease)

Murica_105

Murica_106

Murica_107

Murica_108
(helix-turn-helix DNA binding protein)

Murica_109
(HNH endonuclease)

Murica_110

Murica_113

Murica_114

Murica_115

Murica_116

Murica_117
(DnaQ exonuclease (DNA polymerase III subunit))

Murica_118

Murica_119
(RecA DNA Recombinase)

Murica_120

Murica_121

Murica_122

Murica_123

Murica_124

Murica_125

Murica_126

Murica_127

Murica_128

Murica_129

Murica_130

Murica_131

Murica_132
(helix-turn-helix DNA binding domain)

Murica_133

Murica_134

Murica_135

Murica_136

Murica_137

Murica_138

Murica_139

Murica_140

Murica_141

Murica_142

Murica_143

Murica_144

Murica_145

Murica_146

Murica_147

Murica_148

Murica_149
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF919525
Refseq NumberNone yet
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload