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Mycobacterium phage Simpliphy
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Detailed Information for Phage Simpliphy
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByGretchen Walch
Year Found2012
Location FoundAlexandria, KY Campbell County
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates38.93909 N, 84.37412 W Map
Discovery NotesSoil was captured on the edge of a creek, 50 cm (about)from the closest water edge; water was moving but congested at the nearest point to the water. The air temperature at the time was about 26.1 degrees Celsius. The area surrounding the sample location was muddy and interspersed with grass, plat roots, and an earth worm was captured, but releases upon collecting the sample. It was within 30 cm of larger creek vegetation/weeds and flowers. The location was approximately 115.7 cm under a wood bridge thus receiving shade throughout the day at differing times and intensity. The closest tree was within approximately 4-5 meters of the location. The man made things in the area included a bed of concrete within 115.5 cm the nearest road was approximately 16 meters away. The location was aptly located in a sort of valley with hills facing East and West with the creek itself running South to North. It is known to have frequent flooding reaching 2-3 meters of the creek edge (thus the soil sample location is within the flood zone). Finally, it is known that the creek could have septic run-off from the surrounding houses running along the road. The creek itself stems from a pond 1.5 miles South of the sample location and runs from the pond North (1.5 miles) to the sample capture location. The creek is exposed to the run-off of 5 West facing houses (2 of which are on the hill of the valley) and 6 East facing houses all of which are on the East facing hill. (houses are within 400 yards and are in rural spacing.)
Naming NotesThe name of Simpliphy came from the idea that phages are in essence simple as in small and not complex in structure. However, despite this they have many possible applications that can be very helpful. For example, wound healing.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMay 23, 2013
Sequencing FacilityVirginia Commonwealth University Nucleic Acids Research Facilities
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage112
Genome length (bp)75366
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content63.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Other Cluster Members
MorphotypeSiphoviridae
Final annotation complete?No
Has been Phamerated?Yes
Gene List

Click to View

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Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE
Available Files
EM PictureDownload