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Mycobacterium phage Simpliphy
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Detailed Information for Phage Simpliphy
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByGretchen Walch
Year Found2012
Location FoundAlexandria, KY Campbell County
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates38.93909 N, 84.37412 W Map
Discovery NotesSoil was captured on the edge of a creek, 50 cm (about)from the closest water edge; water was moving but congested at the nearest point to the water. The air temperature at the time was about 26.1 degrees Celsius. The area surrounding the sample location was muddy and interspersed with grass, plat roots, and an earth worm was captured, but releases upon collecting the sample. It was within 30 cm of larger creek vegetation/weeds and flowers. The location was approximately 115.7 cm under a wood bridge thus receiving shade throughout the day at differing times and intensity. The closest tree was within approximately 4-5 meters of the location. The man made things in the area included a bed of concrete within 115.5 cm the nearest road was approximately 16 meters away. The location was aptly located in a sort of valley with hills facing East and West with the creek itself running South to North. It is known to have frequent flooding reaching 2-3 meters of the creek edge (thus the soil sample location is within the flood zone). Finally, it is known that the creek could have septic run-off from the surrounding houses running along the road. The creek itself stems from a pond 1.5 miles South of the sample location and runs from the pond North (1.5 miles) to the sample capture location. The creek is exposed to the run-off of 5 West facing houses (2 of which are on the hill of the valley) and 6 East facing houses all of which are on the East facing hill. (houses are within 400 yards and are in rural spacing.)
Naming NotesThe name of Simpliphy came from the idea that phages are in essence simple as in small and not complex in structure. However, despite this they have many possible applications that can be very helpful. For example, wound healing.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMay 23, 2013
Sequencing FacilityVirginia Commonwealth University Nucleic Acids Research Facilities
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage112
Genome length (bp)75366
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content63.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusIn GenBank
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

Simpliphy_1

Simpliphy_2

Simpliphy_3

Simpliphy_4

Simpliphy_5
(terminase small subunit)

Simpliphy_6
(membrane protein)

Simpliphy_7

Simpliphy_8

Simpliphy_9
(terminase large subunit)

Simpliphy_10

Simpliphy_11
(portal protein)

Simpliphy_12
(capsid maturation protease)

Simpliphy_13
(major capsid protein)

Simpliphy_14
(head-to-tail connector complex protein)

Simpliphy_15
(head-to-tail connector complex protein)

Simpliphy_16
(head-to-tail connector complex protein)

Simpliphy_17
(head-to-tail connector complex protein)

Simpliphy_18
(head-to-tail connector complex protein)

Simpliphy_19
(major tail protein)

Simpliphy_20
(capsid decoration protein)

Simpliphy_21
(tail assembly chaperone)

Simpliphy_22
(tail assembly chaperone)

Simpliphy_23
(tape measure protein)

Simpliphy_24
(minor tail protein)

Simpliphy_25
(minor tail protein)

Simpliphy_26
(minor tail protein)

Simpliphy_27
(minor tail protein)

Simpliphy_28
(D-ala-D-ala carboxypeptidase)

Simpliphy_29
(minor tail protein)

Simpliphy_30

Simpliphy_31
(helix-turn-helix DNA binding domain protein)

Simpliphy_32
(membrane protein)

Simpliphy_33
(lysin A)

Simpliphy_34
(holin)

Simpliphy_35

Simpliphy_36
(lysin B)

Simpliphy_37
(membrane protein)

Simpliphy_38
(NrdH-like glutaredoxin)

Simpliphy_39

Simpliphy_40
(Lsr2-like DNA bridging protein)

Simpliphy_41

Simpliphy_42

Simpliphy_43

Simpliphy_44

Simpliphy_45

Simpliphy_46

Simpliphy_47

Simpliphy_48

Simpliphy_49

Simpliphy_50
(membrane protein)

Simpliphy_51
(integrase)

Simpliphy_52

Simpliphy_53
(membrane protein)

Simpliphy_54
(immunity repressor)

Simpliphy_55

Simpliphy_56
(membrane protein)

Simpliphy_57
(pentapeptide repeat protein)

Simpliphy_58

Simpliphy_59

Simpliphy_60

Simpliphy_61

Simpliphy_62

Simpliphy_63

Simpliphy_64

Simpliphy_65

Simpliphy_66

Simpliphy_67

Simpliphy_68
(ERF family ssDNA binding protein)

Simpliphy_69

Simpliphy_70

Simpliphy_71

Simpliphy_72

Simpliphy_73
(membrane protein)

Simpliphy_74

Simpliphy_75
(DprA-like DNA processing chain A)

Simpliphy_76
(HNH endonuclease)

Simpliphy_77

Simpliphy_78

Simpliphy_79

Simpliphy_80
(DnaB-like dsDNA helicase)

Simpliphy_81

Simpliphy_82
(WhiB family transcription factor)

Simpliphy_83

Simpliphy_84

Simpliphy_85
(holliday junction resolvase)

Simpliphy_86
(membrane protein)

Simpliphy_87
(polynucleotide kinase)

Simpliphy_88

Simpliphy_89
(membrane protein)

Simpliphy_90
(RNA ligase)

Simpliphy_91
(helix-turn-helix DNA binding domain protein)

Simpliphy_92
(WhiB family transcription factor)

Simpliphy_93

Simpliphy_94

Simpliphy_95

Simpliphy_96

Simpliphy_97

Simpliphy_98
(DNA methylase)

Simpliphy_99
(ssDNA binding protein)

Simpliphy_100
(ClpP-like protease)

Simpliphy_101

Simpliphy_102

Simpliphy_103
(helix-turn-helix DNA binding domain protein)

Simpliphy_104
(HNH endonuclease)

Simpliphy_107

Simpliphy_108

Simpliphy_109
(hydrolase)

Simpliphy_110

Simpliphy_111
(DNA polymerase III subunit)

Simpliphy_112

Simpliphy_113
(RecA-like DNA recombinase)

Simpliphy_114

Simpliphy_115

Simpliphy_116

Simpliphy_117

Simpliphy_118

Simpliphy_119

Simpliphy_120

Simpliphy_121

Simpliphy_122

Simpliphy_123

Simpliphy_124

Simpliphy_125

Simpliphy_126
(helix-turn-helix DNA binding domain protein)

Simpliphy_127

Simpliphy_128

Simpliphy_129

Simpliphy_130

Simpliphy_131

Simpliphy_132

Simpliphy_133

Simpliphy_134

Simpliphy_135

Simpliphy_136

Simpliphy_137

Simpliphy_138

Simpliphy_139

Simpliphy_140

Simpliphy_141

Simpliphy_142

Simpliphy_143

Simpliphy_144

Simpliphy_145

Simpliphy_146

Simpliphy_147

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH536827
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
Pitt Freezer Box# 43
Pitt Freezer Box Grid# 16
Available Files
EM PictureDownload
GenBank File for PhameratorDownload