Mycobacterium phage Bruin
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Detailed Information for Phage Bruin
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByFeldman LT, McCracken J, Ku J, Ji S, et al.
Year Found2008
Location FoundLos Angeles, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramUnavailable
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates34.0 N, 118.0 W Map
Discovery NotesCollection date: January 14, 2008
Sample type: Soil
Sample descriptors: Compost/mulch pile on UCLA campus
Depth of sample: 3 inches below surface
Temperature: 60°F
pH of soil: 5-6
Additional information: SAmple collected by Lawrence T. Feldman. Phage isolation performed by Sheng Ji, David Nguyen, Ting-Chang Sheu, Andy Tran, Jeong-hee Ku, and Jenna McCracken (winter 2008). DNA preparation performed by Krisanavane Reddi. Assembly performed by William Villella (UCLA) and confirmed by Daniel Russell (University of Pittsburgh). Preliminary genome annotation performed by Emma Goodwin, Mohammad Hamad, Eden Maloney, and Viviana Quintana (winter 2013). Curation of annotation results performed by Shannon Cooper, Khristina Ipapo, and Tiffany Chow (spring 2013; UCLA) as well as Debbie Jacobs-Sera (summer 2013; University of Pittsburgh).
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMay 13, 2013
Sequencing FacilityJoint Genomics Institute
Shotgun Sequencing MethodSanger Sequencing
Approximate Shotgun Coverage46
Sanger Finishing Reactions7
Genome length (bp)74210
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content63.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesDescription: Clear with turbid halos
Average size: 5 mm diameter (with range of 3.8 mm to 7.3 mm diameter)
MorphotypeSiphoviridae
Number of Genes141
Number of tRNAs2
Has been Phamerated?Yes
Gene List

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Bruin_1

Bruin_2

Bruin_3

Bruin_4

Bruin_5
(terminase small subunit)

Bruin_6

Bruin_7

Bruin_8
(terminase large subunit)

Bruin_9

Bruin_10
(portal protein)

Bruin_11
(capsid maturation protease)

Bruin_12
(major capsid protein)

Bruin_13

Bruin_14

Bruin_15

Bruin_16

Bruin_17

Bruin_18
(major capsid protein)

Bruin_19
(tail assembly chaperone)

Bruin_20
(tail assembly chaperone)

Bruin_21
(HNH endonuclease)

Bruin_22
(tapemeasure)

Bruin_23
(minor tail protein)

Bruin_24
(minor tail protein)

Bruin_25
(minor tail protein)

Bruin_26
(minor tail protein)

Bruin_27
(D-ala-D-ala carboxypeptidase)

Bruin_28

Bruin_29

Bruin_30

Bruin_31

Bruin_32
(LysA)

Bruin_33
(holin)

Bruin_34

Bruin_35
(LysB)

Bruin_36

Bruin_37
(NrdH)

Bruin_38
(Lsr2-like protein)

Bruin_39

Bruin_40

Bruin_41

Bruin_42

Bruin_43

Bruin_44

Bruin_45

Bruin_46

Bruin_47

Bruin_48
(integrase (Y-Int))

Bruin_49

Bruin_50

Bruin_51

Bruin_52

Bruin_53

Bruin_54
(pentapeptide repeat protein)

Bruin_55

Bruin_56

Bruin_57

Bruin_58

Bruin_59

Bruin_60

Bruin_61

Bruin_62

Bruin_63

Bruin_64

Bruin_65

Bruin_66
(Erf)

Bruin_67

Bruin_68

Bruin_69

Bruin_70

Bruin_71

Bruin_72

Bruin_73

Bruin_74
(HNH domain protein)

Bruin_75

Bruin_76

Bruin_77

Bruin_78
(DnaB-like helicase)

Bruin_79

Bruin_80

Bruin_81

Bruin_82

Bruin_83

Bruin_84

Bruin_85
(Pnk)

Bruin_86

Bruin_87

Bruin_88
(RNA ligase)

Bruin_89

Bruin_90
(WhiB)

Bruin_91

Bruin_92

Bruin_93

Bruin_94

Bruin_95

Bruin_96
(DNA methylase)

Bruin_97
(ssDNA binding protein)

Bruin_98
(Clp protease)

Bruin_99

Bruin_100

Bruin_101

Bruin_102

Bruin_103

Bruin_106

Bruin_107

Bruin_108

Bruin_109

Bruin_110
(DnaQ-like protein)

Bruin_111

Bruin_112
(RecA)

Bruin_113

Bruin_114

Bruin_115

Bruin_116

Bruin_117

Bruin_118

Bruin_119

Bruin_120

Bruin_121

Bruin_122

Bruin_123

Bruin_124

Bruin_125

Bruin_126

Bruin_127

Bruin_128

Bruin_129

Bruin_130

Bruin_131

Bruin_132

Bruin_133

Bruin_134

Bruin_135

Bruin_136

Bruin_137

Bruin_138

Bruin_139

Bruin_140

Bruin_141

Bruin_142

Bruin_143

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKF562099
Refseq NumberNC_022988
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.35e11
Date of SEA Lysate Titering May 14, 2008
Pitt Freezer Box# 3
Pitt Freezer Box Grid# D10
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload