Mycobacterium phage Gemini
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Detailed Information for Phage Gemini
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByEric Westerman
Year Found2010
Location Foundrural, WV USA
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates38.770916 N, 81.31445 W Map
Discovery NotesThe soil type was fertile garden soil.
I dug 8.89 centimeters into the soil.
The temperature was 68 degrees Fahrenheit.
On top of a large hill in West Virginia, near Spenser, WV.

I found the sample in a vegetable garden which cantained peppers and tomatoes. Being in the area of a former pasture field and a current residential area, the ground was relatively flat, and the soil must have been tread on often during the gardening process. The garden was surrounded by a fence, but other than that, the closest structure was a driveway, which was approximately 25ft away. The soil was relatively moist, and it should yield some interesting phage do to its fertility.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMar 1, 2016
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2875
Genome length (bp)75649
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content62.9%
Fasta file available?Yes: Download fasta file
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusBeing Annotated (Expected completion by 8/31/2018)
Plaque Notes0.4cm clear plaques with turbid haloes, and also 0.1cm plaques that look clear at first glance, but on closer inspection also have thinner haloes. Of particular note is the unwillingness of plaques to overtake each other, usually forming around instead of over other plaques. Best seen in relationship between big halo and small plaques. Forms half moon shape. 24 hour incubation period in 37°C conditions.
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Designator 2010WEKUgeminiEWW
SEA Lysate Titer 7.4e10
Date of SEA Lysate Titering Oct 5, 2010
Pitt Freezer Box# 20
Pitt Freezer Box Grid# 1
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload