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Mycobacterium phage Youngblood
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Detailed Information for Phage Youngblood
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByTeam Bad Assay
Year Found2014
Location FoundLos Angeles, CA United States
Finding InstitutionUniversity of California, Los Angeles
ProgramUnavailable
From enriched soil sample?Yes
Isolation Temperature35°C
GPS Coordinates34.075 N, 118.451 W Map
Discovery NotesPhage isolation performed in Spring 2014 and genome annotation performed in Fall 2014 by Team Bad Assay: Arash Amighi, Chaya Patel, Yvonne Lam, Steven Youngblood. Annotation quality checked by Edric Yoon and Lei Yeh. DNA preparation performed by Dr. Krisanavane Reddi (UCLA). Assembly performed by Dr. Jordan Moberg Parker (UCLA).

Sample description: Dry, dark brown, grainy soil collected at a 6cm depth by Yvonne Lam on April 4, 2014. Area is urban and flat. The location is near trees and foot traffic.
Naming NotesNamed after team member Steven Youngblood.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 23, 2015
Sequencing FacilityThe UCLA Genotyping and Sequencing Core
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage38
Genome length (bp)75896
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content62.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Other Cluster Members
Plaque NotesThe plaque morphology had a bullseye or turbid morphology depending on the dilution. The plaques were circular ranging from 0.2 to 0.5 cm with the average size of 3.37 mm and standard deviation of 0.43 mm.
MorphotypeSiphoviridae
Final annotation complete?No
Number of Genes148
Number of tRNAs2
Has been Phamerated?Yes
Gene List

Click to View

Youngblood_1

Youngblood_2

Youngblood_3

Youngblood_4

Youngblood_5
(terminase, small subunit)

Youngblood_6

Youngblood_7

Youngblood_8
(terminase, large subunit)

Youngblood_9

Youngblood_10
(portal protein)

Youngblood_11
(capsid maturation protease)

Youngblood_12
(major capsid protein)

Youngblood_13
(head-to-tail connector protein)

Youngblood_14
(head-to-tail connector protein)

Youngblood_15
(head-to-tail connector protein)

Youngblood_16
(head-to-tail connector protein)

Youngblood_17
(head-to-tail connector protein)

Youngblood_18
(major tail protein)

Youngblood_19

Youngblood_20
(tail assembly chaperone)

Youngblood_21
(tail assembly chaperone)

Youngblood_22
(tape measure protein)

Youngblood_23
(minor tail protein)

Youngblood_24
(minor tail protein)

Youngblood_25
(minor tail protein)

Youngblood_26
(minor tail protein)

Youngblood_27
(minor tail protein, D-ala-D-ala carboxypeptidase)

Youngblood_28
(minor tail protein)

Youngblood_29

Youngblood_30
(helix-turn-helix DNA binding domain)

Youngblood_31

Youngblood_32
(lysin A)

Youngblood_33
(holin)

Youngblood_34

Youngblood_35
(lysin B)

Youngblood_36

Youngblood_37
(NrdH glutaredoxin)

Youngblood_38
(Lsr2 DNA bridging protein)

Youngblood_39

Youngblood_40

Youngblood_41

Youngblood_42

Youngblood_43

Youngblood_44

Youngblood_45

Youngblood_46

Youngblood_47

Youngblood_48

Youngblood_49

Youngblood_50

Youngblood_51

Youngblood_52

Youngblood_53
(membrane domain protein)

Youngblood_54
(integrase, (Y-int))

Youngblood_55

Youngblood_56

Youngblood_57
(immunity repressor)

Youngblood_58

Youngblood_59

Youngblood_60
(pentapeptide repeat protein)

Youngblood_61

Youngblood_62

Youngblood_63

Youngblood_64

Youngblood_65

Youngblood_66

Youngblood_67

Youngblood_68

Youngblood_69

Youngblood_70

Youngblood_71

Youngblood_72

Youngblood_73

Youngblood_74

Youngblood_75

Youngblood_76

Youngblood_77

Youngblood_78

Youngblood_79

Youngblood_80
(HNH endonuclease)

Youngblood_81

Youngblood_82

Youngblood_83

Youngblood_84
(DnaB dsDNA helicase)

Youngblood_85

Youngblood_86
(WhiB family transcription factor)

Youngblood_87

Youngblood_88

Youngblood_89
(holliday-junction resolvase)

Youngblood_90

Youngblood_91
(polynucleotide kinase)

Youngblood_92

Youngblood_93

Youngblood_94
(RNA ligase)

Youngblood_95
(helix-turn-helix DNA binding protein)

Youngblood_96
(WhiB family transcription factor)

Youngblood_97

Youngblood_98

Youngblood_99

Youngblood_100

Youngblood_101

Youngblood_102
(DNA methylase)

Youngblood_103
(ssDNA binding protein)

Youngblood_104
(ClpP protease)

Youngblood_105

Youngblood_106

Youngblood_107

Youngblood_108
(helix-turn-helix DNA binding protein)

Youngblood_109
(HNH endonuclease)

Youngblood_110

Youngblood_113

Youngblood_114

Youngblood_115

Youngblood_116

Youngblood_117
(DnaQ exonuclease (DNA polymerase III subunit))

Youngblood_118

Youngblood_119
(RecA DNA Recombinase)

Youngblood_120

Youngblood_121

Youngblood_122

Youngblood_123

Youngblood_124

Youngblood_125

Youngblood_126

Youngblood_127

Youngblood_128

Youngblood_129

Youngblood_130

Youngblood_131

Youngblood_132
(helix-turn-helix DNA binding domain)

Youngblood_133

Youngblood_134

Youngblood_135

Youngblood_136

Youngblood_137

Youngblood_138

Youngblood_139

Youngblood_140

Youngblood_141

Youngblood_142

Youngblood_143

Youngblood_144

Youngblood_145

Youngblood_146

Youngblood_147

Youngblood_148

Youngblood_149

Youngblood_150
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload