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Mycobacterium phage Youngblood
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Detailed Information for Phage Youngblood
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByTeam Bad Assay
Year Found2014
Location FoundLos Angeles, CA United States
Finding InstitutionUniversity of California, Los Angeles
ProgramUnavailable
From enriched soil sample?Yes
Isolation Temperature35°C
GPS Coordinates34.075 N, 118.451 W Map
Discovery NotesPhage isolation performed in Spring 2014 and genome annotation performed in Fall 2014 by Team Bad Assay: Arash Amighi, Chaya Patel, Yvonne Lam, Steven Youngblood. Annotation quality checked by Edric Yoon and Lei Yeh. DNA preparation performed by Dr. Krisanavane Reddi (UCLA). Assembly performed by Dr. Jordan Moberg Parker (UCLA).

Sample description: Dry, dark brown, grainy soil collected at a 6cm depth by Yvonne Lam on April 4, 2014. Area is urban and flat. The location is near trees and foot traffic.
Naming NotesNamed after team member Steven Youngblood.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 23, 2015
Sequencing FacilityThe UCLA Genotyping and Sequencing Core
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage38
Genome length (bp)75896
Character of genome ends3' Sticky Overhang
Overhang Length9 bp
Overhang SequenceCGCTTGTCA
GC Content62.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Other Cluster Members
Plaque NotesThe plaque morphology had a bullseye or turbid morphology depending on the dilution. The plaques were circular ranging from 0.2 to 0.5 cm with the average size of 3.37 mm and standard deviation of 0.43 mm.
MorphotypeSiphoviridae
Final annotation complete?No
Number of Genes148
Number of tRNAs2
Has been Phamerated?Yes
Gene List

Click to View

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Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload