Mycobacterium phage Icee
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Detailed Information for Phage Icee
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByEmily Ice
Year Found2016
Location FoundLouisville, KY United States
Finding InstitutionSpalding University
ProgramKentucky Biomedical Infrastructure Network Small Genomes Discovery Program
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates38.258611 N, 85.748056 W Map
Discovery NotesFound by the Ohio River in downtown Louisville. It was a sunny day and the soil was moist. The temperature was 50 degrees Fahrenheit.
Naming NotesMy last name is unique so I decided to name my phage after it.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMar 14, 2017
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1393
Genome length (bp)74169
Character of genome ends3' Sticky Overhang
Overhang Length9 bases
Overhang SequenceCGCTTGTCA
GC Content63.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterE
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesLytic and 1mm in diameter.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Icee_1

Icee_2

Icee_3

Icee_4

Icee_5

Icee_6
(terminase, small subunit)

Icee_7

Icee_8
(terminase, large subunit)

Icee_9

Icee_10
(portal protein)

Icee_11
(capsid maturation protease)

Icee_12
(major capsid protein)

Icee_13
(head-to-tail connector complex protein)

Icee_14
(head-to-tail connector complex protein)

Icee_15
(head-to-tail connector complex protein)

Icee_16
(head-to-tail connector complex protein)

Icee_17
(head-to-tail connector complex protein)

Icee_18
(major tail protein)

Icee_19
(tail assembly chaperone)

Icee_20
(tail assembly chaperone)

Icee_21
(tape measure protein)

Icee_22
(minor tail protein)

Icee_23
(minor tail protein)

Icee_24
(minor tail protein)

Icee_25
(minor tail protein)

Icee_26
(minor tail protein, D-ala-D-ala carboxypeptidase)

Icee_27
(minor tail protein)

Icee_28

Icee_29
(helix-turn-helix DNA binding domain)

Icee_30
(membrane protein)

Icee_31
(lysin A)

Icee_32
(holin)

Icee_33

Icee_34
(lysin B)

Icee_35
(NrdH-like glutaredoxin)

Icee_36
(NrdH-like glutaredoxin)

Icee_37
(Lsr2-like DNA bridging protein)

Icee_38
(Lsr2-like DNA bridging protein)

Icee_39

Icee_40

Icee_41

Icee_42

Icee_43

Icee_44

Icee_45

Icee_46

Icee_47

Icee_48

Icee_49

Icee_50

Icee_51
(membrane protein)

Icee_52
(integrase, (Y-int))

Icee_53

Icee_54
(membrane protein)

Icee_55
(immunity repressor)

Icee_56

Icee_57
(membrane protein)

Icee_58
(pentapeptide repeat protein)

Icee_59

Icee_60

Icee_61

Icee_62

Icee_63

Icee_64

Icee_65

Icee_66

Icee_67

Icee_68

Icee_69

Icee_70
(ssDNA binding protein, ERF family)

Icee_71

Icee_72

Icee_73

Icee_74

Icee_75
(membrane protein)

Icee_76

Icee_77
(DprA-like DNA processing chain A)

Icee_78
(HNH endonuclease)

Icee_79

Icee_80

Icee_81

Icee_82
(DNAB-like dsDNA helicase)

Icee_83

Icee_84
(WhiB family transcription factor)

Icee_85

Icee_86

Icee_87
(RuvC-like resolvase)

Icee_88
(membrane protein)

Icee_89
(polynucleotide kinase)

Icee_90

Icee_91
(membrane protein)

Icee_92
(RNA ligase)

Icee_93
(helix-turn-helix DNA binding domain)

Icee_94
(WhiB family transcription factor)

Icee_95

Icee_96

Icee_97

Icee_98

Icee_99

Icee_100
(endonuclease VII)

Icee_101
(ssDNA binding protein)

Icee_102
(ClpP-like protease)

Icee_103

Icee_104

Icee_105

Icee_106
(excise)

Icee_107
(HNH endonuclease)

Icee_110

Icee_111

Icee_112
(HNH endonuclease)

Icee_113
(hydrolase)

Icee_114
(NKF)

Icee_115
(DnaQ-like exonuclease (DNA polymerase III subunit))

Icee_116

Icee_117

Icee_118

Icee_119

Icee_120
(RecA-like DNA Recombinase)

Icee_121

Icee_122

Icee_123

Icee_124

Icee_125

Icee_126

Icee_127

Icee_128

Icee_129

Icee_130

Icee_131

Icee_132
(helix-turn-helix DNA binding domain)

Icee_133
(ParB-like dsDNA partitioning protein)

Icee_134

Icee_135

Icee_136

Icee_137

Icee_138

Icee_139

Icee_140

Icee_141

Icee_142

Icee_143

Icee_144

Icee_145

Icee_146
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH399778
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 4.8x10^10 pfu/mL
Pitt Freezer Box# 28
Pitt Freezer Box Grid# B8
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload