The Actinobacteriophage Database

Mycobacterium phage Hopey

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Detailed Information for Phage Hopey
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Allison, Anthony + Miranda
Year Found	2013
Location Found	North Andover, MA USA
Finding Institution	Merrimack College
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	Not entered
GPS Coordinates	42.669331 N, 71.124094 W Map
Discovery Notes	From a truck tire track on the Merrimack College campus.
Naming Notes	Our president, Christopher Hopey.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Dec 10, 2013
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina
Sequencer Used	Illumina MiSeq
Read Type	Single-end reads
Read Length	150 bp
Approximate Shotgun Coverage	3442
Genome length (bp)	75586
Character of genome ends	3' Sticky Overhang
Overhang Length	9 bases
Overhang Sequence	CGCTTGTCA
GC Content	63.0%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	E
Subcluster	--
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View 244 ABCat Adnama Amao AmericanBeauty Argent26 Asriel BaboJay BadStone Balomoji Barbarian Bask21 Becksu Bench BigBubba BilboSwaggins Bruin Buck BugsBunny Cactus ChosenOne ChotaBhai Cjw1 Command613 Contagion Cookies CrystalP Czyszczon1 Daikon DoctorDiddles DrDrey Dumbo Dusk Easy2Say Elite2014 Elph10 Emmina Eureka FairyPath Filch FireRed Flypotenuse FoghornLeghorn Gage Gator Gemini Glexan Goku GoldenSpark Goldilocks GooberAzure HanKaySha Harella Henry Highbury Holt Hoonter Hopey HufflyPuff Icee IHOP Inca JeTaime Kanye Kimchi Kostya Lilac Lilizi Lilpickle MadamMonkfish Manda Marshmallow Maxxinista Mindy Miniwave Misfit MISSy Moldemort Mosby MPhalcon Murica Murphy Myrale Nala NelitzaMV Nimrod NoSleep OrionPax Palpatine Paperbeatsrock Pat3 Petra64142 Phaja Pharsalus PhatBacter Phaux Phrux Policronamos Porky Pumpkin Quallification Rakim Rimmer RiverMonster Saints25 Sassay ShamWow ShereKhan Simpliphy SirDuracell SophKB Sotrice96 Stank Stark StellaBean StolenFromERC StormChicken Tarkin TBrady12 TeardropMSU Teaspoon Terminus Thimble Thresher Tomaszewski Toto Traaww1 Tuco Ukulele Wiggin Willez Xandras xkcd YassJohnny Youngblood
Annotating Institution	Merrimack College
Annotation Status	In GenBank
Plaque Notes	Cloudy with rings around the outer edge
Morphotype	Siphoviridae
Number of Genes	142
Number of tRNAs	2
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View Hopey_1 Hopey_2 Hopey_3 Hopey_4 Hopey_5 Hopey_6 (terminase small subunit) Hopey_7 Hopey_8 Hopey_9 (terminase large subunit) Hopey_10 Hopey_11 (portal protein) Hopey_12 (capsid maturation protease) Hopey_13 (major capsid protein) Hopey_14 Hopey_15 (head-to-tail adaptor) Hopey_16 (head-to-tail stopper) Hopey_17 Hopey_18 (tail terminator) Hopey_19 (major tail protein) Hopey_20 Hopey_21 (tail assembly chaperone) Hopey_22 (tail assembly chaperone) Hopey_23 (endonuclease VII) Hopey_24 (tape measure protein) Hopey_25 (minor tail protein) Hopey_26 (minor tail protein) Hopey_27 (minor tail protein) Hopey_28 (minor tail protein) Hopey_29 (D-Ala-D-Ala carboxypeptidase) Hopey_30 Hopey_31 Hopey_32 (helix-turn-helix DNA binding domain protein) Hopey_33 Hopey_34 (lysin A) Hopey_35 (holin) Hopey_36 Hopey_37 (lysin B) Hopey_38 Hopey_39 (NrdH-like glutaredoxin) Hopey_40 Hopey_41 (Lsr2-like DNA bridging protein) Hopey_42 Hopey_43 Hopey_44 Hopey_45 Hopey_46 Hopey_47 Hopey_48 Hopey_49 Hopey_50 Hopey_51 Hopey_52 (tyrosine integrase) Hopey_53 Hopey_54 Hopey_55 (immunity repressor) Hopey_56 Hopey_57 Hopey_58 (pentapeptide repeat protein) Hopey_59 Hopey_60 Hopey_61 Hopey_62 Hopey_63 Hopey_64 Hopey_65 Hopey_66 Hopey_67 (exonuclease) Hopey_68 Hopey_69 (ERF family ssDNA binding protein) Hopey_70 Hopey_71 Hopey_72 Hopey_73 Hopey_74 Hopey_75 Hopey_76 (DprA-like DNA processing chain A) Hopey_77 (HNH endonuclease) Hopey_78 Hopey_79 Hopey_80 Hopey_81 (DnaB-like dsDNA helicase) Hopey_82 Hopey_83 (WhiB family transcription factor) Hopey_84 Hopey_85 Hopey_86 (holliday junction resolvase) Hopey_87 Hopey_88 (polynucleotide kinase) Hopey_89 Hopey_90 Hopey_91 Hopey_92 (RNA ligase) Hopey_93 (helix-turn-helix DNA binding domain protein) Hopey_94 (WhiB family transcription factor) Hopey_95 Hopey_96 Hopey_97 Hopey_98 Hopey_99 Hopey_100 (DNA methylase) Hopey_101 (ssDNA binding protein) Hopey_102 (ClpP-like protease) Hopey_103 Hopey_104 Hopey_105 (MerR-like helix-turn-helix DNA binding domain protein) Hopey_106 (HNH endonuclease) Hopey_107 — tRNA Hopey_108 — tRNA Hopey_109 Hopey_110 Hopey_111 (hydrolase) Hopey_112 Hopey_113 (DnaQ-like DNA polymerase III subunit) Hopey_114 Hopey_115 (RecA-like DNA recombinase) Hopey_116 Hopey_117 Hopey_118 Hopey_119 Hopey_120 Hopey_121 Hopey_122 Hopey_123 Hopey_124 Hopey_125 Hopey_126 Hopey_127 (helix-turn-helix DNA binding domain protein) Hopey_128 Hopey_129 Hopey_130 Hopey_131 Hopey_132 Hopey_133 Hopey_134 Hopey_135 Hopey_136 Hopey_137 Hopey_138 Hopey_139 Hopey_140 Hopey_141 Hopey_142 Hopey_143 Hopey_144 (HNH endonuclease)
Submitted Annotation in DNA Master Format	Download
Submitted Minimal DNA Master File	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MH576953
Refseq Number	None yet
Published in Paper	Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	14
Pitt Freezer Box Grid#	G9
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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