| Detailed Information for Phage ElvisPhasley |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Savannah Prozik |
| Year Found | 2024 |
| Location Found | Williamsburg, VA United States |
| Finding Institution | College of William & Mary |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 20°C |
| GPS Coordinates | 37.27039 N, 76.71756 W Map |
| Discovery Notes | At the location I collected my soil sample, the soil was very wet and muddy. The location was directly next to the right side of the pond behind the Randolph building, which can be accessed from the forest entrance when walking toward Sadler. I collected about two tablespoons of soil, dipping about an inch down from the surface. |
| Naming Notes | I chose this name due to my obsession with Elvis Presley and quirky scientific puns. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 15, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 6513 |
| Genome length (bp) | 69502 |
| Character of genome ends | Circularly Permuted |
| GC Content | 66.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | B |
| Subcluster | B1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | College of William & Mary |
| Annotation Status | QC Complete |
| Plaque Notes | As seen by the image below, my phage created small halo-like plaques that were about 1 mm in length throughout the lawn. For the majority of my agar plates, there has been minimal turbidity presented compared to clear web lysis. Despite having to dilute my phage much further than expected, the size and shape of the plaques created by my phage were very similar. |
| Morphotype | Siphoviridae |
| Number of Genes | 102 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
208 |
| Pitt Freezer Box Grid# |
G6 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
