Mycobacterium phage Robyn
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Detailed Information for Phage Robyn
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByConnor Wallace
Year Found2018
Location FoundAuckland, New Zealand
Finding InstitutionMassey University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature37°C
GPS Coordinates36.766111 S, 174.688611 E Map
Discovery NotesThis bacteriophage was isolated from a sample of damp soil underneath a mulch of composted grass-clippings.
Naming NotesThis phage was named in tribute to a deceased family pet, a chicken named Robyn.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJul 19, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1096
Genome length (bp)68467
Character of genome endsCircularly Permuted
GC Content66.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterB
SubclusterB1
Cluster Life CycleLytic
Other Cluster Members

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AbsoluteMadLad (B1)

ABU (B1)

Adriana (B1)

Aelin (B1)

AltPhacts (B1)

Altwerkus (B1)

Anderson (B1)

Andre (B1)

Antonia (B1)

Apizium (B1)

Ashraf (B1)

Badfish (B1)

Banjo (B1)

Basato (B1)

BatteryCK (B1)

Beaglebox (B1)

Bishoperium (B1)

BlackStallion (B1)

BlueHusk (B1)

Bluephacebaby (B1)

Boehler (B1)

Brilliant (B1)

Buckeye (B1)

Burr (B1)

CamL (B1)

CampRoach (B1)

Cannibal (B1)

Carthage (B1)

Chaelin (B1)

Chah (B1)

Charles1 (B1)

CharlieGBrown (B1)

CheetO (B1)

Cher (B1)

Childish (B1)

Chorkpop (B1)

Chunky (B1)

Chute (B1)

Cobra (B1)

Colbert (B1)

Coletti (B1)

Cornobble (B1)

Cosmolli16 (B1)

Craff (B1)

Crownjwl (B1)

DaddyDaniels (B1)

Daffy (B1)

Daka (B1)

Dati (B1)

DelRivs (B1)

Derpp (B1)

Dice (B1)

Dingo (B1)

Dione (B1)

DirtJuice (B1)

Doddsville (B1)

DoesntMatter (B1)

DonSanchon (B1)

DuchessDung (B1)

Duggie (B1)

Durga (B1)

Emiris (B1)

EmpTee (B1)

Eremos (B1)

Etaye (B1)

Eugenia (B1)

Fang (B1)

FluffyNinja (B1)

Fozzie (B1)

Frankicide (B1)

Freya (B1)

FriarPreacher (B1)

Fringe (B1)

FugateOSU (B1)

Gareth (B1)

GeneCoco (B1)

Giraffe (B1)

Gophee (B1)

Grand2040 (B1)

Gwilliam (B1)

Gyarad (B1)

Haimas (B1)

Haleema (B1)

Hamish (B1)

Hartsy (B1)

Harvey (B1)

Held (B1)

HenryJackson (B1)

Hertubise (B1)

Hetaeria (B1)

HighStump (B1)

Hocus (B1)

Horchata (B1)

HSavage (B1)

ImtiyazSitla (B1)

Inchworm (B1)

Inverness (B1)

Iridoclysis (B1)

IsaacEli (B1)

JacAttac (B1)

JakeO (B1)

JangoPhett (B1)

JDog (B1)

Jillium (B1)

Jiminy (B1)

Jiraiya (B1)

Kahve (B1)

Kailash (B1)

Katniss (B1)

Keitherie (B1)

Kikipoo (B1)

Kimbrough (B1)

KingTut (B1)

KingVeVeVe (B1)

KlimbOn (B1)

Kloppinator (B1)

KLucky39 (B1)

Kwadwo (B1)

Kwksand96 (B1)

Labeouficaum (B1)

LasagnaCat (B1)

Lasso (B1)

LeeLot (B1)

Lego3393 (B1)

Legolas (B1)

LeiMonet (B1)

LemonSlice (B1)

Longacauda (B1)

Lopsy (B1)

LostAndPhound (B1)

LuckyMarjie (B1)

Lulumae (B1)

Lulwa (B1)

Lumine (B1)

Mag7 (B1)

Magic8 (B1)

Mana (B1)

Manad (B1)

Maru (B1)

Maskar (B1)

Matalotodo (B1)

Mcshane (B1)

Mecca (B1)

Megamind20 (B1)

Megatron (B1)

Melc17 (B1)

MelsMeow (B1)

Mesh1 (B1)

MichaelPhcott (B1)

Mikota (B1)

MiniBoss (B1)

MitKao (B1)

Morgushi (B1)

Morty (B1)

Mosaic (B1)

MRabcd (B1)

MrPhizzler (B1)

Mulan (B1)

Murdoc (B1)

Mutante (B1)

Nacho (B1)

Newman (B1)

Nicole21 (B1)

Numberten (B1)

Nyala (B1)

Olak (B1)

Oline (B1)

Olive (B1)

OliverWalter (B1)

Omniscient (B1)

Oosterbaan (B1)

Orfeu (B1)

Orion (B1)

Orwigg (B1)

OSmaximus (B1)

Pacifista (B1)

PG1 (B1)

Phamished (B1)

Phareon (B1)

PhatCats2014 (B1)

PhatLouie (B1)

PhenghisKhan (B1)

Pherdinand (B1)

Phergie (B1)

Phipps (B1)

Phleuron (B1)

PhrankReynolds (B1)

PhrodoBaggins (B1)

Phunky (B1)

Piglet (B1)

PinheadLarry (B1)

Pinkman (B1)

Pipsqueak (B1)

Placalicious (B1)

Plmatters (B1)

Podrick (B1)

Pops (B1)

Potter (B1)

Prickles (B1)

ProfessorX (B1)

Puhltonio (B1)

QueenBeane (B1)

Quisquiliae (B1)

RedMaple (B1)

Ricotta (B1)

Riggan (B1)

Rimu (B1)

Robyn (B1)

Roliet (B1)

Roscoe (B1)

Roy17 (B1)

Samaymay (B1)

SassyCat97 (B1)

Schadenfreude (B1)

Schueller (B1)

Scoot17C (B1)

Scrick (B1)

SDcharge11 (B1)

Selr12 (B1)

Serendipity (B1)

Serpentine (B1)

Sheila (B1)

ShiVal (B1)

Sigman (B1)

SilverBell (B1)

Simielle (B1)

Skippy (B1)

Slatt (B1)

Soile (B1)

Solosis (B1)

Sophia (B1)

Soto (B1)

Spartan300 (B1)

Squid (B1)

Squiggle (B1)

Struggle (B1)

Suffolk (B1)

Surely (B1)

Swiphy (B1)

Swish (B1)

TallGrassMM (B1)

Telesworld (B1)

Thora (B1)

ThreeOh3D2 (B1)

Thunderbird (B1)

Timmi (B1)

TomBombadil (B1)

Tomlarah (B1)

Toni (B1)

Tooj (B1)

True (B1)

Trypo (B1)

TyrionL (B1)

UAch1 (B1)

UncleHowie (B1)

Usavi (B1)

Vaishali24 (B1)

Valjean (B1)

Vaticameos (B1)

Veritas (B1)

Virapocalypse (B1)

Virgeve (B1)

Vista (B1)

Vivaldi (B1)

Vortex (B1)

Wallhey (B1)

Waterdiva (B1)

Weher20 (B1)

Windsor (B1)

Xavier (B1)

Yoshand (B1)

YouGoGlencoco (B1)

Zaider (B1)

Zelda (B1)

Zonia (B1)

Imvubu (B10)

Indlovu (B11)

LilMcDreamy (B12)

BirdsNest (B13)

Hashim76 (B13)

Zenteno07 (B13)

Allegro (B2)

Arbiter (B2)

Ares (B2)

Bananafish (B2)

Blocker23 (B2)

Boyle (B2)

Brownie5 (B2)

Calamitous (B2)

Coffee (B2)

Eaglehorse (B2)

Faze9 (B2)

FrenchFry (B2)

Glass (B2)

Godines (B2)

Hedgerow (B2)

Holeinone (B2)

ItsyBitsy1 (B2)

Kaleb (B2)

Kheth (B2)

Lars (B2)

Laurie (B2)

Lephleur (B2)

LizLemon (B2)

MasterPo (B2)

Opia (B2)

Phantasmagoria (B2)

Qyrzula (B2)

Rhinoforte (B2)

Rosebush (B2)

Sabella (B2)

TA17A (B2)

Tinciduntsolum (B2)

Tres (B2)

Vic9 (B2)

West99 (B2)

Abinghost (B3)

Akoma (B3)

Athena (B3)

Audrey (B3)

Baloo (B3)

Ben11 (B3)

Bernardo (B3)

Briakila (B3)

Casbah (B3)

ChaChing (B3)

Chandler (B3)

Compostia (B3)

Corofin (B3)

Daisy (B3)

Deenasa (B3)

Devonte (B3)

Gadjet (B3)

Gervas (B3)

GlenHope (B3)

Halfpint (B3)

HarveySr (B3)

Heathcliff (B3)

Jackstina (B3)

Kamiyu (B3)

Kronus (B3)

LestyG (B3)

Marley1013 (B3)

MmasiCarm (B3)

Mortcellus (B3)

Morty007 (B3)

Neos5 (B3)

Nozo (B3)

Obutu (B3)

OrangeOswald (B3)

Phaedrus (B3)

Phayeta (B3)

Philly (B3)

Phlyer (B3)

Pipefish (B3)

RagingRooster (B3)

Rita1961 (B3)

RomaT (B3)

SlippinJimmy (B3)

SynergyX (B3)

Tydolla (B3)

Yahalom (B3)

Yinz (B3)

Ahwei (B4)

AlanGrant (B4)

Antihero (B4)

Apex (B4)

Austelle (B4)

BobbyK (B4)

BrownCNA (B4)

ChrisnMich (B4)

Cooper (B4)

Epah (B4)

Fanaw (B4)

Fortunato (B4)

Frederick (B4)

GinPorsche (B4)

Hally898 (B4)

Hangman (B4)

Heath (B4)

Hydro (B4)

JaguarMi (B4)

JAMaL (B4)

Lambano (B4)

Lolalove (B4)

Magpie (B4)

Mithril (B4)

Mudslide (B4)

Nanao (B4)

Nigel (B4)

Poster (B4)

Prince (B4)

QueenPie (B4)

RawrgerThat (B4)

Stinger (B4)

Vincenzo (B4)

VioletZ (B4)

Waleliano (B4)

Xincheng (B4)

Zemanar (B4)

Acadian (B5)

Baee (B5)

DigitDog (B5)

Donny (B5)

EastView9101 (B5)

KarasumaRum (B5)

Mysterious (B5)

Nangang (B5)

Nehemiah (B5)

Phelemich (B5)

Reprobate (B5)

Rich (B5)

Serendipitous (B5)

Shenyu (B5)

SirJeffery (B5)

StinkyVeggy (B5)

Suigeneris (B5)

39HC (B6)

40BC (B6)

Hosp (B6)

Jolie1 (B6)

KayaCho (B6)

Saguaro (B7)

Thonko (B8)

CRB2 (B9)

Funsized (B9)

Knocker (B9)

PenguinLover67 (B9)

Quesadilla (B9)

Rinkes (B9)

Annotating InstitutionMassey University
Annotation StatusIn GenBank
Plaque NotesPlaques measure at ~0.1 cm in diameter.
Plaques are exclusively clear.
MorphotypeSiphoviridae
Number of Genes102
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Robyn_1
(adenylate kinase)

Robyn_2
(terminase)

Robyn_3

Robyn_4

Robyn_5

Robyn_6
(RuvC-like resolvase)

Robyn_7

Robyn_8
(portal protein)

Robyn_9
(capsid maturation protease and MuF-like fusion protein)

Robyn_10

Robyn_11
(major capsid hexamer protein)

Robyn_12
(major capsid pentamer protein)

Robyn_13

Robyn_14

Robyn_15

Robyn_16

Robyn_17
(major tail protein)

Robyn_18

Robyn_19
(queuine tRNA-ribosyltransferase)

Robyn_20

Robyn_21
(head-to-tail adaptor)

Robyn_22

Robyn_23

Robyn_24
(tail assembly chaperone)

Robyn_25

Robyn_26

Robyn_27
(tape measure protein)

Robyn_28
(minor tail protein)

Robyn_29
(minor tail protein)

Robyn_30
(minor tail protein)

Robyn_31
(minor tail protein)

Robyn_32
(minor tail protein)

Robyn_33

Robyn_34

Robyn_35

Robyn_36

Robyn_37

Robyn_38

Robyn_39

Robyn_40

Robyn_41

Robyn_42

Robyn_43

Robyn_44
(helix-turn-helix DNA binding domain protein)

Robyn_45
(helix-turn-helix DNA binding domain protein)

Robyn_46

Robyn_47
(lysin A)

Robyn_48
(lysin B)

Robyn_49

Robyn_50
(exonuclease)

Robyn_51
(DNA helicase)

Robyn_52

Robyn_53

Robyn_54

Robyn_55

Robyn_56

Robyn_57
(DNA primase/helicase)

Robyn_58

Robyn_59

Robyn_60
(DNA polymerase I)

Robyn_61

Robyn_62

Robyn_63

Robyn_64

Robyn_65

Robyn_66

Robyn_67

Robyn_68

Robyn_69

Robyn_70

Robyn_71

Robyn_72

Robyn_73

Robyn_74

Robyn_75

Robyn_76

Robyn_77

Robyn_78

Robyn_79

Robyn_80

Robyn_81

Robyn_82

Robyn_83

Robyn_84

Robyn_85

Robyn_86
(HNH endonuclease)

Robyn_87

Robyn_88

Robyn_89

Robyn_90

Robyn_91

Robyn_92

Robyn_93

Robyn_94

Robyn_95

Robyn_96

Robyn_97

Robyn_98

Robyn_99

Robyn_100

Robyn_101

Robyn_102

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK524526
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1x10^12
Pitt Freezer Box# 72
Pitt Freezer Box Grid# D8
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload