Detailed Information for Phage Ading |
Discovery Information |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Nicholas Dingler |
Year Found | 2016 |
Location Found | Philadelphia, PA USA |
Finding Institution | La Salle University |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | Not entered |
GPS Coordinates | 40.037778 N, 75.149167 W Map |
Discovery Notes | The sample was taken from the middle of the La Salle University Campus next to our College Hall building. It was a soil sample from about 6 inches below the surface. The temperature of the phages environment was approximately 70 degrees. The phage was very cooperative when growing. |
Naming Notes | I first chose two soil samples and named the two, A and B. A, was the one where I found my phage. A, was the first component of my name. Next, I took the first four letters of my last name and integrated the two. This is how "Ading" was born. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Feb 9, 2017 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina Sequencing |
Approximate Shotgun Coverage | 47 |
Genome length (bp) | 157399 |
Character of genome ends | Circularly Permuted |
GC Content | 64.6% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | C |
Subcluster | C1 |
Cluster Life Cycle | Lytic |
Other Cluster Members |
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Annotating Institution | La Salle University |
Annotation Status | In GenBank |
Plaque Notes | The plaque is approximately 3/4 cm in diameter. The edges of the plaque were rough and bumpy. |
Morphotype | Myoviridae |
Has been Phamerated? | Yes |
Gene List |
|
Submitted Annotation in DNA Master Format | Download |
Submitted Minimal DNA Master File | Download |
Author List | Download |
Annotation Cover Sheet | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | MK359347 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Not in Pitt Archives |
SEA Lysate Titer |
28,000 |
Date of SEA Lysate Titering |
Sep 29, 2016 |
Available Files |
Restriction Digest Picture | Download |
Final DNAMaster File | Download |
GenBank File for Phamerator | Download |