The Actinobacteriophage Database

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Detailed Information for Phage Phox
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	David Parra
Year Found	2016
Location Found	Walnut City, CA USA
Finding Institution	University of California, Los Angeles
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	No
Isolation Temperature	35°C
GPS Coordinates	34.04 N, 117.84 W Map
Discovery Notes	Phage Phox was isolated from dry soil collected at a depth of 4cm on 10/2/16 by David Parra. Air temp at time of collection was 18C. Phox was isolated by Direct plating at 35C. Characterization was perform by Team Plaque Hole (David Parra, Christopher Hsu, Quan Le, Karen Lam) and Team Phage and Phurious (Gregory Rosenberg, Lifan Jin, Jonathan Hwang, Daniel Shen) Phage Phox was annotated by Team Plaque Hole, Team Phage and Phurious, Team Phaged and Confused (Alexis Doherty, Alexis Dowiak, Regis Fallon, Joshua Green), and Team Phage-A Vu (Marco Aquinde, Jennifer Bergsma, Thuytien Duong, Catherine Le). Instructor: Dr. Jordan Moberg Parker Teaching Assistants: Michelle Bina & Andrew Lund Undergrad Assistant: Samuel Wu Laboratory Support: Dr. Krisanavane Reddi.
Naming Notes	Karen's favorite animal is Fox which pronounces like Phox
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Dec 20, 2016
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina Sequencing
Approximate Shotgun Coverage	402
Genome length (bp)	154874
Character of genome ends	Circularly Permuted
GC Content	64.7%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	C
Subcluster	C1
Cluster Life Cycle	Lytic
Other Cluster Members	Click to View Ading (C1) Adlitam (C1) Alice (C1) Amataga (C1) ArcherS7 (C1) Astraea (C1) Atlantean (C1) Audrick (C1) Ava3 (C1) BadAgartude (C1) Bangla1971 (C1) Basquiat (C1) BeanWater (C1) Bigswole (C1) Bipolarisk (C1) Blackbrain (C1) Bonray (C1) Bread (C1) Breeniome (C1) Burrough (C1) Bxz1 (C1) Cactojaque (C1) Cali (C1) Cane17 (C1) Catera (C1) Chargie21 (C1) CharlieB (C1) ChickenPhender (C1) Colt (C1) Daffodil (C1) Dandelion (C1) Darko (C1) Delilah (C1) Derek (C1) Dietrick (C1) Drazdys (C1) DrPhinkDaddy (C1) DTDevon (C1) EmmaElysia (C1) EmToTheThree (C1) Erdmann (C1) ErnieJ (C1) Essence (C1) ET08 (C1) Ewok (C1) Flabslab (C1) Fludd (C1) FoxtrotP1 (C1) FrayBell (C1) FudgeTart (C1) Gabriel (C1) Ghost (C1) Gizmo (C1) Grungle (C1) Guwapp (C1) HyRo (C1) InigoMontoya (C1) InterFolia (C1) Iota (C1) Jessibeth14 (C1) JPickles (C1) JustHall (C1) Kamryn (C1) Kboogie (C1) Khaleesi (C1) Koguma (C1) LifeSavor (C1) LinStu (C1) Littleton (C1) Loanshark (C1) LolaVinca (C1) LRRHood (C1) Lukilu (C1) Mangeria (C1) McWolfish (C1) Megamind (C1) Melpomini (C1) MikeLiesIn (C1) Momo (C1) MoMoMixon (C1) Morizzled23 (C1) Naija (C1) Napoleon13 (C1) Nappy (C1) Nidhogg (C1) NoodleTree (C1) Norm (C1) NuevoMundo (C1) ParkTD (C1) Patter (C1) Phlegm (C1) Phox (C1) Phusco (C1) Pier (C1) Pinkcreek (C1) Pio (C1) Pivoine (C1) Pleione (C1) QBert (C1) Rabinovish (C1) Rahel (C1) Rizal (C1) RoMag (C1) Roots515 (C1) Salacia (C1) Sauce (C1) ScottMcG (C1) Sebata (C1) Shaqnato (C1) Shelob (C1) ShiaLabeouf (C1) Shnickers (C1) Shrimp (C1) SmallFry (C1) Specks (C1) Sprinklers (C1) Spud (C1) StephanieG (C1) Stubby (C1) Teardrop (C1) TinyTim (C1) Tonenili (C1) Tortoise16 (C1) Trinitium (C1) Tyke (C1) ValleyTerrace (C1) Wally (C1) Willis (C1) YoungMoneyMata (C1) Yucca (C1) Zalkecks (C1) Zeenon (C1) ZygoTaiga (C1) Myrna (C2) Phabba (C2)
Annotation Status	In GenBank
Plaque Notes	1mm pinpoint plaques
Morphotype	Myoviridae
Has been Phamerated?	Yes
Gene List	Click to View Phox_1 Phox_2 (membrane domain protein) Phox_3 (nucleotidyltransferase) Phox_4 Phox_5 Phox_6 Phox_7 Phox_8 Phox_9 Phox_10 Phox_11 Phox_12 Phox_13 Phox_14 Phox_15 Phox_16 Phox_17 Phox_18 Phox_19 Phox_20 Phox_21 (membrane domain protein) Phox_22 Phox_23 Phox_24 Phox_25 Phox_26 Phox_27 Phox_28 (band-7-like membrane protein) Phox_29 Phox_30 (helix-turn-helix DNA binding domain protein) Phox_31 Phox_32 Phox_33 Phox_34 Phox_35 Phox_36 Phox_37 Phox_38 Phox_39 Phox_40 Phox_41 Phox_42 Phox_43 Phox_44 (helix-turn-helix DNA binding domain protein) Phox_45 (helix-turn-helix DNA binding domain protein) Phox_46 (membrane domain protein) Phox_47 Phox_48 Phox_49 Phox_50 Phox_51 Phox_52 Phox_53 Phox_54 (helix-turn-helix DNA binding domain protein) Phox_55 Phox_56 Phox_57 Phox_58 Phox_59 Phox_60 Phox_61 (helix-turn-helix DNA binding domain protein) Phox_62 (helix-turn-helix DNA binding domain protein) Phox_63 Phox_64 Phox_65 Phox_66 Phox_67 Phox_68 Phox_69 Phox_70 Phox_71 Phox_72 Phox_73 Phox_74 Phox_75 Phox_76 Phox_77 Phox_78 Phox_79 Phox_85 Phox_86 Phox_87 (portal protein) Phox_88 Phox_89 Phox_90 (thymidylate synthase) Phox_91 Phox_92 (RNA ligase) Phox_93 (LysM-like endolysin) Phox_94 Phox_95 Phox_96 (major capsid protein) Phox_97 (endonuclease) Phox_98 Phox_99 Phox_100 Phox_101 Phox_102 Phox_103 Phox_104 Phox_105 (membrane protein) Phox_106 Phox_107 Phox_108 Phox_109 Phox_110 Phox_111 Phox_112 (capsid decoration protein) Phox_113 Phox_114 Phox_115 Phox_116 Phox_117 Phox_118 Phox_119 Phox_120 Phox_121 Phox_122 Phox_123 Phox_124 (tail sheath protein) Phox_125 Phox_126 Phox_127 (tail assembly chaperone) Phox_128 (tail assembly chaperone) Phox_129 (tapemeasure protein) Phox_130 Phox_131 Phox_132 Phox_133 (minor tail protein) Phox_134 Phox_135 (minor tail protein) Phox_136 (baseplate wedge protein) Phox_137 (baseplate J protein) Phox_138 (HNH endonuclease) Phox_139 (HNH endonuclease) Phox_140 Phox_141 (membrane domain protein) Phox_142 Phox_143 (minor tail protein) Phox_144 Phox_145 Phox_146 Phox_147 Phox_148 Phox_149 Phox_150 Phox_154 Phox_155 (peptidyl-tRNA hydrolase) Phox_173 (minor tail protein) Phox_174 Phox_187 Phox_188 (DNA helicase) Phox_189 Phox_190 Phox_191 Phox_192 Phox_193 Phox_194 Phox_195 Phox_196 Phox_197 Phox_198 (thymidylate kinase) Phox_199 (aminotransferase) Phox_200 Phox_201 Phox_202 Phox_203 (helicase loader) Phox_204 (dsDNA helicase) Phox_205 Phox_206 (DNA primase) Phox_207 Phox_208 (DnaJ-like chaperonin) Phox_209 Phox_210 (DNA polymerase III alpha subunit) Phox_211 (RF-1 peptide chain release factor) Phox_212 (DNA recombinase) Phox_213 (holliday junction resolvase) Phox_214 Phox_215 (exonuclease) Phox_216 (resolvase) Phox_217 Phox_218 Phox_219 Phox_220 Phox_221 Phox_222 Phox_223 Phox_226 Phox_227 Phox_228 Phox_229 Phox_230 Phox_231 Phox_232 Phox_233 Phox_234 (RNA binding protein) Phox_235 Phox_236 Phox_237 Phox_238 Phox_239 Phox_240 Phox_241 (glycosyltransferase) Phox_242 (membrane protein) Phox_243 (glycosyltransferase) Phox_244 (galactosyltransferase) Phox_245 (nicotinamide riboside transport protein) Phox_246 (glycosyltransferase) Phox_247 Phox_248 Phox_249 Phox_250 Phox_251 (lysin A) Phox_252 (holin) Phox_253 (lysin B) Phox_254 (terminase) Phox_255 Phox_256 Phox_257 Phox_258 Phox_259 (polynucleotide kinase) Phox_260 Phox_261 Phox_262 (serine/threonine kinase) Phox_263 Phox_264 Phox_265 (PurA-like adenylosuccinate synthetase) Phox_266 Phox_267 Phox_268
Submitted Minimal DNA Master File	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MF919527
Refseq Number	None yet
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	45
Pitt Freezer Box Grid#	D1
Available Files
GenBank File for Phamerator	Download

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