| Detailed Information for Phage Ading |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Nicholas Dingler |
| Year Found | 2016 |
| Location Found | Philadelphia, PA USA |
| Finding Institution | La Salle University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.037778 N, 75.149167 W Map |
| Discovery Notes | The sample was taken from the middle of the La Salle University Campus next to our College Hall building. It was a soil sample from about 6 inches below the surface. The temperature of the phages environment was approximately 70 degrees. The phage was very cooperative when growing. |
| Naming Notes | I first chose two soil samples and named the two, A and B. A, was the one where I found my phage. A, was the first component of my name. Next, I took the first four letters of my last name and integrated the two. This is how "Ading" was born. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 9, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 47 |
| Genome length (bp) | 157399 |
| Character of genome ends | Circularly Permuted |
| GC Content | 64.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | C |
| Subcluster | C1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | La Salle University |
| Annotation Status | In GenBank |
| Plaque Notes | The plaque is approximately 3/4 cm in diameter. The edges of the plaque were rough and bumpy. |
| Morphotype | Myoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK359347 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| SEA Lysate Titer |
28,000 |
| Date of SEA Lysate Titering |
Sep 29, 2016 |
| Available Files |
| Restriction Digest Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |