Mycobacterium phage Ading
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Detailed Information for Phage Ading
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByNicholas Dingler
Year Found2016
Location FoundPhiladelphia, PA USA
Finding InstitutionLa Salle University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates40.037778 N, 75.149167 W Map
Discovery NotesThe sample was taken from the middle of the La Salle University Campus next to our College Hall building. It was a soil sample from about 6 inches below the surface. The temperature of the phages environment was approximately 70 degrees. The phage was very cooperative when growing.
Naming NotesI first chose two soil samples and named the two, A and B. A, was the one where I found my phage. A, was the first component of my name. Next, I took the first four letters of my last name and integrated the two. This is how "Ading" was born.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 9, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage47
Genome length (bp)157399
Character of genome endsCircularly Permuted
GC Content64.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Cluster Life CycleLytic
Other Cluster Members

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Ading (C1)

Adlitam (C1)

Alice (C1)

Amataga (C1)

ArcherS7 (C1)

Astraea (C1)

Atlantean (C1)

Audrick (C1)

Ava3 (C1)

BadAgartude (C1)

Bangla1971 (C1)

Basquiat (C1)

BeanWater (C1)

Bigswole (C1)

Bipolarisk (C1)

Blackbrain (C1)

Bonray (C1)

Bread (C1)

Breeniome (C1)

Burrough (C1)

Bxz1 (C1)

Cactojaque (C1)

Cali (C1)

Cane17 (C1)

Catera (C1)

Chargie21 (C1)

CharlieB (C1)

ChickenPhender (C1)

Colt (C1)

Daffodil (C1)

Dandelion (C1)

Darko (C1)

Delilah (C1)

Derek (C1)

Dietrick (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Flabslab (C1)

Fludd (C1)

FoxtrotP1 (C1)

FrayBell (C1)

FudgeTart (C1)

Gabriel (C1)

Ghost (C1)

Gizmo (C1)

Grungle (C1)

Guwapp (C1)

HyRo (C1)

InigoMontoya (C1)

InterFolia (C1)

Iota (C1)

Jessibeth14 (C1)

JPickles (C1)

JustHall (C1)

Kamryn (C1)

Kboogie (C1)

Khaleesi (C1)

Koguma (C1)

LifeSavor (C1)

LinStu (C1)

Littleton (C1)

Loanshark (C1)

LolaVinca (C1)

LRRHood (C1)

Lukilu (C1)

Mangeria (C1)

McWolfish (C1)

Megamind (C1)

Melpomini (C1)

MikeLiesIn (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

Naija (C1)

Napoleon13 (C1)

Nappy (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

ParkTD (C1)

Patter (C1)

Phlegm (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pinkcreek (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

QBert (C1)

Rabinovish (C1)

Rahel (C1)

Rizal (C1)

RoMag (C1)

Roots515 (C1)

Salacia (C1)

Sauce (C1)

ScottMcG (C1)

Sebata (C1)

Shaqnato (C1)

Shelob (C1)

ShiaLabeouf (C1)

Shifa (C1)

Shnickers (C1)

Shrimp (C1)

SmallFry (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

StephanieG (C1)

Stubby (C1)

Teardrop (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Trinitium (C1)

Tyke (C1)

ValleyTerrace (C1)

Wally (C1)

Willis (C1)

YoungMoneyMata (C1)

Yucca (C1)

Zalkecks (C1)

Zeenon (C1)

ZygoTaiga (C1)

Myrna (C2)

Phabba (C2)

Annotating InstitutionLa Salle University
Annotation StatusIn GenBank
Plaque NotesThe plaque is approximately 3/4 cm in diameter. The edges of the plaque were rough and bumpy.
MorphotypeMyoviridae
Has been Phamerated?Yes
Gene List

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Ading_1

Ading_2

Ading_3
(HNH endonuclease)

Ading_4

Ading_5

Ading_6

Ading_7

Ading_8

Ading_9

Ading_10

Ading_11

Ading_12

Ading_13

Ading_14

Ading_15

Ading_16

Ading_17
(histidine triad nucleotide binding protein)

Ading_18

Ading_19

Ading_20

Ading_21

Ading_22

Ading_23

Ading_24

Ading_25
(HNH endonuclease)

Ading_26

Ading_27

Ading_28

Ading_29

Ading_30

Ading_31
(helix-turn-helix DNA binding protein)

Ading_32

Ading_33

Ading_34

Ading_35
(ribbon-helix-helix DNA binding domain protein)

Ading_36

Ading_37

Ading_38

Ading_39

Ading_40

Ading_41

Ading_42

Ading_43

Ading_44

Ading_45

Ading_46

Ading_47

Ading_48

Ading_49

Ading_50

Ading_51

Ading_52

Ading_53

Ading_54

Ading_55
(helix-turn-helix DNA binding protein)

Ading_56

Ading_57

Ading_58

Ading_59

Ading_60

Ading_61

Ading_62
(helix-turn-helix DNA binding domain protein)

Ading_63
(helix-turn-helix DNA binding domain protein)

Ading_64

Ading_65

Ading_66

Ading_67

Ading_68

Ading_69

Ading_70

Ading_71

Ading_72

Ading_73

Ading_74

Ading_75

Ading_76

Ading_77

Ading_78

Ading_79

Ading_80

Ading_81

Ading_82

Ading_83

Ading_88
(methyltransferase)

Ading_89

Ading_90
(portal protein)

Ading_91

Ading_92

Ading_93
(ThyX-like thymidylate synthase)

Ading_94

Ading_95
(RNA ligase)

Ading_96
(HNH endonuclease)

Ading_97
(LysM domain protein)

Ading_98

Ading_99

Ading_100
(major capsid protein)

Ading_101
(capsid decoration protein)

Ading_102

Ading_103

Ading_104

Ading_105

Ading_106

Ading_107

Ading_108
(membrane protein)

Ading_109

Ading_110

Ading_111

Ading_112

Ading_113

Ading_114

Ading_115

Ading_116

Ading_117

Ading_118

Ading_119

Ading_120

Ading_121
(N-acetyltransferase)

Ading_122

Ading_123

Ading_124

Ading_125

Ading_126

Ading_127
(tail sheath protein)

Ading_128

Ading_129

Ading_130
(tail assembly chaperone)

Ading_131
(tail assembly chaperone)

Ading_132
(tape measure protein)

Ading_133
(minor tail protein)

Ading_134

Ading_135

Ading_136
(minor tail protein)

Ading_137

Ading_138

Ading_139
(baseplate wedge protein)

Ading_140
(baseplate wedge protein)

Ading_141

Ading_142

Ading_143

Ading_144
(minor tail protein)

Ading_145
(minor tail protein)

Ading_146

Ading_147

Ading_148

Ading_149

Ading_150

Ading_151

Ading_152

Ading_153

Ading_157

Ading_158
(peptidyl tRNA hydrolase)

Ading_164
(HNH endonuclease)

Ading_173
(HNH endonuclease)

Ading_175
(D-ala-D-ala carboxypeptidase)

Ading_176

Ading_187

Ading_188
(helicase)

Ading_189

Ading_190

Ading_191

Ading_192

Ading_193

Ading_194

Ading_195

Ading_196

Ading_197

Ading_198
(thymidylate kinase)

Ading_199
(aminotransferase)

Ading_200
(RNA binding domain protein)

Ading_201

Ading_202

Ading_203
(DnaC-like helicase loader)

Ading_204
(DnaB-like dsDNA helicase)

Ading_205

Ading_206
(DNA primase)

Ading_207

Ading_208
(DnaJ-like chaperonin)

Ading_209
(ssDNA binding domain protein)

Ading_210
(DNA polymerase III (alpha))

Ading_211
(RF-1 peptide chain release factor)

Ading_212
(RecA-like DNA recombinase)

Ading_213
(holliday junction resolvase)

Ading_214

Ading_215
(HNH endonuclease)

Ading_216
(RusA-like resolvase)

Ading_217

Ading_218

Ading_219

Ading_220

Ading_221
(glycoside hydrolase)

Ading_222

Ading_223
(HNH endonuclease)

Ading_224

Ading_227

Ading_228

Ading_229

Ading_230

Ading_231

Ading_232

Ading_233

Ading_234

Ading_235
(Ro-like RNA binding protein)

Ading_236

Ading_237

Ading_238

Ading_239

Ading_240

Ading_241

Ading_242
(galactosaminyltransferase)

Ading_243

Ading_244
(glycosyltransferase)

Ading_245
(galactosyltransferase)

Ading_246
(nicotinate ribosyltransferase)

Ading_247
(glycosyltransferase)

Ading_248

Ading_249

Ading_250

Ading_251

Ading_252
(HNH endonuclease)

Ading_253
(lysin A)

Ading_254
(membrane protein)

Ading_255
(lysin B)

Ading_256
(terminase)

Ading_257

Ading_258

Ading_259

Ading_260

Ading_261
(polynucleotide kinase)

Ading_262

Ading_263
(serine/threonine kinase)

Ading_264

Ading_265

Ading_266
(adenylosuccinate synthetase)

Ading_267

Ading_268

Ading_269

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK359347
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
SEA Lysate Titer 28,000
Date of SEA Lysate Titering Sep 29, 2016
Available Files
Restriction Digest PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload