| Detailed Information for Phage Yassified |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Owen Koetters |
| Year Found | 2022 |
| Location Found | Los Angeles, CA United States |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 35°C |
| GPS Coordinates | 34.006988 N, 118.46831 W Map |
| Discovery Notes | Sample was moist and in an area replete with organic waste due to domesticated dogs and rats that live in the area.
Soil environment is well-shaded, densely packed with vegetables, and is thoroughly irrigated. Weather was sunny at the time of sampling.
Plated on MHA plate with 20 mM CaCl2 |
| Naming Notes | By characterizing Yassified, we actively yassified it, which means to beautify or make something substantially better, which is done via isolation and through the process of visualizing the phage. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jul 26, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 167 |
| Genome length (bp) | 155851 |
| Character of genome ends | Circularly Permuted |
| GC Content | 64.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | C |
| Subcluster | C1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | SUNY Old Westbury |
| Annotation Status | In GenBank |
| Plaque Notes | Clear plaques with a diameter that ranged from 1-2mm (1.5 mm avg) |
| Morphotype | Myoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR613471 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
156 |
| Pitt Freezer Box Grid# |
A3 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
