Mycobacterium phage IkeLoa
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Detailed Information for Phage IkeLoa
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByA. Beach, J. Angeles, S. Kushner, T. Gray
Year Found2021
Location FoundLongview, TX United States
Finding InstitutionLeTourneau University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates32.465224 N, 94.726598 W Map
Discovery NotesThe weather outside for the discovery day was cloudy with a 50% chance of rain. The high that day was 33.9°C and the low was 23.9°C. We found Ikeloa underneath rich, moist, black mulch around bushes. In the surrounding area were worms and lots of organismal life.
Naming NotesThe SEA-PHAGES program that our school did was an incredible learning experience and we could not be more grateful for the opportunities to take part in such valuable research. The Hawaiian term "'Ike Loa" refers to the value of learning and knowledge, of growth and expanding your mind. We thought this beautiful phrase perfectly encapsulated our journey of phage-hunting and how appreciative we are to school and God that we may learn and grow in truth and knowledge.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 1, 2021
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage233
Genome length (bp)155280
Character of genome endsCircularly Permuted
GC Content64.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Cluster Life CycleLytic
Other Cluster Members

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Ading (C1)

Adlitam (C1)

Alice (C1)

Amataga (C1)

ArcherS7 (C1)

Asterius (C1)

Astraea (C1)

Atlantean (C1)

Audrick (C1)

Ava3 (C1)

Babyland (C1)

BackyardAgain (C1)

BadAgartude (C1)

BananaFence (C1)

Bangla1971 (C1)

Basquiat (C1)

BeanWater (C1)

BigCity (C1)

Bigswole (C1)

Bipolarisk (C1)

Blackbrain (C1)

Blackdragon (C1)

Blubba (C1)

Bonray (C1)

Bread (C1)

Breeniome (C1)

Burrough (C1)

Bxz1 (C1)

Cactojaque (C1)

Cali (C1)

Cane17 (C1)

Capablanca (C1)

Caravan (C1)

Catera (C1)

Chargie21 (C1)

CharlieB (C1)

ChaylaJr (C1)

ChickenPhender (C1)

Chomp (C1)

CindyLou (C1)

Colt (C1)

Concombre (C1)

Daffodil (C1)

Dandelion (C1)

Darko (C1)

Delilah (C1)

Delylah (C1)

Demodog (C1)

Derek (C1)

Dietrick (C1)

DirtMonster (C1)

Domi (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EasyJones (C1)

EggyFarm (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Flabslab (C1)

Fludd (C1)

FoxtrotP1 (C1)

FrayBell (C1)

FudgeTart (C1)

Gabriel (C1)

Gamgam (C1)

Ghost (C1)

Gizmo (C1)

Grasshills (C1)

Grungle (C1)

Guwapp (C1)

Halldule (C1)

HyRo (C1)

I3 (C1)

IkeLoa (C1)

InigoMontoya (C1)

InterFolia (C1)

Iota (C1)

Issimir (C1)

Izajani (C1)

Janiyra (C1)

Jaygup (C1)

JayJay (C1)

Jedediah (C1)

Jemmno (C1)

Jessibeth14 (C1)

JigglyPuff (C1)

JPickles (C1)

JulietS (C1)

JustHall (C1)

Kamryn (C1)

Kboogie (C1)

Khaleesi (C1)

Koguma (C1)

Latch (C1)

Lee7 (C1)

LifeSavor (C1)

LinStu (C1)

Littleton (C1)

Loanshark (C1)

LolaVinca (C1)

LordLeafolot (C1)

LRRHood (C1)

Lukilu (C1)

Maby2021 (C1)

Mangeria (C1)

Matsumoto (C1)

McGee (C1)

McWolfish (C1)

Megamind (C1)

Melpomini (C1)

MikeLiesIn (C1)

Mikro (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

Naija (C1)

Napoleon13 (C1)

Nappy (C1)

Naval22 (C1)

NedWong (C1)

Ngannou (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

ParkTD (C1)

Patter (C1)

Phlegm (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pinkcreek (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

PurduePete (C1)

QBert (C1)

Quasimodo (C1)

Rabinovish (C1)

Rahel (C1)

Rizal (C1)

RoMag (C1)

Ronan (C1)

Roots515 (C1)

Salacia (C1)

Sauce (C1)

ScottMcG (C1)

Sebata (C1)

Shaqnato (C1)

Shelob (C1)

ShiaLabeouf (C1)

Shifa (C1)

Shnickers (C1)

Shrimp (C1)

SilverDipper (C1)

SmallFry (C1)

Spec (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

SrishMeg2525 (C1)

StephanieG (C1)

Stubby (C1)

StubbySaigey (C1)

Tainan (C1)

Teardrop (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Trinitium (C1)

Turret (C1)

Tyke (C1)

ValleyTerrace (C1)

Wally (C1)

Willis (C1)

Yassified (C1)

YemiJoy2021 (C1)

YoungMoneyMata (C1)

Yucca (C1)

Zalkecks (C1)

Zeenon (C1)

ZygoTaiga (C1)

Greely (C2)

Myrna (C2)

Phabba (C2)

ScoobyDoobyDoo (C2)

Annotating InstitutionLeTourneau University
Annotation StatusIn GenBank
Plaque NotesThese plaques were very small in diameter and relatively cloudy. There were no halos, but generally uniform in shape across the entire plate. After taking the average of 10 plaque diameters, we reached an average plaque diameter of 0.7 mm.
MorphotypeMyoviridae
Number of Genes229
Number of tRNAs32
Number of tmRNAs1
Has been Phamerated?Yes
Gene List

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IkeLoa_1

IkeLoa_2

IkeLoa_3
(nucleotidyl transferase)

IkeLoa_4

IkeLoa_5

IkeLoa_6

IkeLoa_7

IkeLoa_8

IkeLoa_9

IkeLoa_10

IkeLoa_11

IkeLoa_12

IkeLoa_13

IkeLoa_14

IkeLoa_15

IkeLoa_16

IkeLoa_17

IkeLoa_18

IkeLoa_19

IkeLoa_20

IkeLoa_21
(HNH endonuclease)

IkeLoa_22

IkeLoa_23

IkeLoa_24

IkeLoa_25
(band-7-like membrane protein)

IkeLoa_26

IkeLoa_27

IkeLoa_28

IkeLoa_29

IkeLoa_30

IkeLoa_31

IkeLoa_32

IkeLoa_33

IkeLoa_34

IkeLoa_35

IkeLoa_36

IkeLoa_37

IkeLoa_38

IkeLoa_39

IkeLoa_40

IkeLoa_41

IkeLoa_42

IkeLoa_43

IkeLoa_44

IkeLoa_45

IkeLoa_46

IkeLoa_47

IkeLoa_48

IkeLoa_49

IkeLoa_50

IkeLoa_51

IkeLoa_52

IkeLoa_53

IkeLoa_54

IkeLoa_55

IkeLoa_56

IkeLoa_57
(helix-turn-helix DNA binding domain protein)

IkeLoa_58
(helix-turn-helix DNA binding domain protein)

IkeLoa_59

IkeLoa_60

IkeLoa_61

IkeLoa_62

IkeLoa_63

IkeLoa_64

IkeLoa_65

IkeLoa_66

IkeLoa_67

IkeLoa_68

IkeLoa_69

IkeLoa_70

IkeLoa_71

IkeLoa_72

IkeLoa_73

IkeLoa_74

IkeLoa_75

IkeLoa_76

IkeLoa_77
(ParB-like nuclease domain protein)

IkeLoa_78 — tRNA
(tRNA-Ser(gct))

IkeLoa_79 — tRNA
(tRNA-Leu(cag))

IkeLoa_80 — tRNA
(tRNA-Leu(gag))

IkeLoa_81 — tRNA
(tRNA-Leu(caa))

IkeLoa_82

IkeLoa_83

IkeLoa_84
(portal protein)

IkeLoa_85

IkeLoa_86

IkeLoa_87
(ThyX-like thymidylate synthase)

IkeLoa_88

IkeLoa_89
(RNA ligase)

IkeLoa_90
(LysM-like peptidoglycan binding protein)

IkeLoa_91

IkeLoa_92

IkeLoa_93
(major capsid protein)

IkeLoa_94
(holliday junction resolvase)

IkeLoa_95

IkeLoa_96
(head fiber protein)

IkeLoa_97

IkeLoa_98

IkeLoa_99

IkeLoa_100

IkeLoa_101

IkeLoa_102

IkeLoa_103

IkeLoa_104

IkeLoa_105

IkeLoa_106

IkeLoa_107

IkeLoa_108

IkeLoa_109

IkeLoa_110

IkeLoa_111

IkeLoa_112

IkeLoa_113

IkeLoa_114
(acetyltransferase)

IkeLoa_115

IkeLoa_116

IkeLoa_117
(ParB-like nuclease domain protein)

IkeLoa_118

IkeLoa_119

IkeLoa_120

IkeLoa_121
(tail sheath protein)

IkeLoa_122

IkeLoa_123

IkeLoa_124
(tail assembly chaperone)

IkeLoa_125
(tail assembly chaperone)

IkeLoa_126
(tape measure protein)

IkeLoa_127
(minor tail protein)

IkeLoa_128

IkeLoa_129

IkeLoa_130
(minor tail protein)

IkeLoa_131

IkeLoa_132

IkeLoa_133
(baseplate wedge protein)

IkeLoa_134
(baseplate J protein)

IkeLoa_135

IkeLoa_136

IkeLoa_137

IkeLoa_138
(minor tail protein)

IkeLoa_139
(minor tail protein)

IkeLoa_140

IkeLoa_141

IkeLoa_142

IkeLoa_143

IkeLoa_144

IkeLoa_145

IkeLoa_146

IkeLoa_147 — tRNA
(tRNA-Pro(tgg))

IkeLoa_148 — tRNA
(tRNA-Trp(cca))

IkeLoa_149 — tRNA
(tRNA-Tyr(gta))

IkeLoa_150

IkeLoa_151
(peptidyl tRNA hydrolase)

IkeLoa_152 — tRNA
(tRNA-Pyl(cta))

IkeLoa_153 — tRNA
(tRNA-Met(cat))

IkeLoa_154 — tRNA
(tRNA-Cys(gca))

IkeLoa_155 — tRNA
(tRNA-Glu(ctc))

IkeLoa_156 — tRNA
(tRNA-His(gtg))

IkeLoa_157 — tRNA
(tRNA-Ala(tgc))

IkeLoa_158 — tRNA
(tRNA-Phe(gaa))

IkeLoa_159 — tRNA
(tRNA-Val(cac))

IkeLoa_160 — tRNA
(tRNA-Lys(ctt))

IkeLoa_161 — tRNA
(tRNA-Glu(ttc))

IkeLoa_162 — tRNA
(tRNA-Gly(tcc))

IkeLoa_163 — tRNA
(tRNA-Thr(cgt))

IkeLoa_164 — tRNA
(ttRNA-Thr(tgt))

IkeLoa_165 — tRNA
(tRNA-Thr(ggt))

IkeLoa_166
(minor tail protein)

IkeLoa_167

IkeLoa_168

IkeLoa_169 — tRNA
(tRNA-Gly(gcc))

IkeLoa_170 — tRNA
(tRNA-Asp(gtc))

IkeLoa_171 — tRNA
(fMet; tRNA-fMet(cat))

IkeLoa_172 — tRNA
(tRNA-Ile(gat))

IkeLoa_173 — tRNA
(tRNA-Arg(acg))

IkeLoa_174 — tRNA
(tRNA-Val(gac))

IkeLoa_175 — tRNA
(tRNA-Arg(cct))

IkeLoa_176 — tmRNA
(Tag peptide: ATDTDATVTDAEIEAFFAEEAAALV*)

IkeLoa_177 — tRNA
(tRNA-Gln(ttg))

IkeLoa_178 — tRNA
(tRNA-Arg(tct))

IkeLoa_179

IkeLoa_180
(DNA helicase)

IkeLoa_181

IkeLoa_182

IkeLoa_183

IkeLoa_184

IkeLoa_185

IkeLoa_186

IkeLoa_187

IkeLoa_188

IkeLoa_189
(thymidylate kinase)

IkeLoa_190
(aminotransferase)

IkeLoa_191

IkeLoa_192

IkeLoa_193

IkeLoa_194
(DnaC-like helicase loader)

IkeLoa_195
(DnaB-like dsDNA helicase)

IkeLoa_196

IkeLoa_197
(DNA primase)

IkeLoa_198

IkeLoa_199
(DnaJ-like chaperonin)

IkeLoa_200
(ssDNA binding protein)

IkeLoa_201
(DnaE-like DNA polymerase III alpha)

IkeLoa_202
(RF-1 peptide chain release factor)

IkeLoa_203
(RecA-like DNA recombinase)

IkeLoa_204
(holliday junction resolvase)

IkeLoa_205

IkeLoa_206
(HNH endonuclease)

IkeLoa_207
(RusA-like resolvase)

IkeLoa_208

IkeLoa_209

IkeLoa_210

IkeLoa_211

IkeLoa_212

IkeLoa_213

IkeLoa_214
(HNH endonuclease)

IkeLoa_215

IkeLoa_216 — tRNA
(tRNA-Gln(ctg))

IkeLoa_217 — tRNA
(tRNA-Asn(gtt))

IkeLoa_218

IkeLoa_219

IkeLoa_220

IkeLoa_221

IkeLoa_222

IkeLoa_223

IkeLoa_224

IkeLoa_225

IkeLoa_226
(Ro-like RNA binding protein)

IkeLoa_227

IkeLoa_228

IkeLoa_229

IkeLoa_230

IkeLoa_231

IkeLoa_232

IkeLoa_233

IkeLoa_234
(glycosyltransferase)

IkeLoa_235

IkeLoa_236
(glycosyltransferase)

IkeLoa_237
(glycosyltransferase)

IkeLoa_238
(PnuC-like nicotinamide riboside transporter)

IkeLoa_239
(glycosyltransferase)

IkeLoa_240

IkeLoa_241

IkeLoa_242

IkeLoa_243

IkeLoa_244
(lysin A)

IkeLoa_245
(holin)

IkeLoa_246
(lysin B)

IkeLoa_247
(terminase)

IkeLoa_248

IkeLoa_249

IkeLoa_250

IkeLoa_251

IkeLoa_252
(polynucleotide kinase)

IkeLoa_253

IkeLoa_254

IkeLoa_255
(serine/threonine kinase)

IkeLoa_256

IkeLoa_257

IkeLoa_258
(PurA-like adenylosuccinate synthetase)

IkeLoa_259

IkeLoa_260

IkeLoa_261

IkeLoa_262

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOP021682
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 134
Pitt Freezer Box Grid# D2
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload