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Mycobacterium phage Colt
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Detailed Information for Phage Colt
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByHaley Mulder and James Gonzalez
Year Found2015
Location FoundCorpus Christi, TX USA
Finding InstitutionDel Mar College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates27.69 N, 97.3784 W Map
Discovery NotesSoil sample was taken in a horse pasture near a small pond on August 31, 2015 at 9:05 AM. Sample was taken 1 1/2 inches under the surface. Sample is a gray clay with small amount of gravel in the soil. Soil contains some algae and has a moist composition. Weather condition was overcast with a slight wind at 1mph, 85% humidity, 0% precipitation, and it was 80 degrees F outside.
Naming NotesPhage was named after my miniature horse named Colt.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 28, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage907
Genome length (bp)158508
Character of genome endsCircularly Permuted
GC Content64.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Cluster Life CycleLytic
Lysogeny NotesFinal DNA concentration identified through spectrophotometry: 217.6 Ng/nl
Other Cluster Members

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Ading (C1)

Adlitam (C1)

Alice (C1)

Amataga (C1)

ArcherS7 (C1)

Astraea (C1)

Atlantean (C1)

Audrick (C1)

Ava3 (C1)

BadAgartude (C1)

Bangla1971 (C1)

Basquiat (C1)

BeanWater (C1)

Bigswole (C1)

Bipolarisk (C1)

Blackbrain (C1)

Bonray (C1)

Bread (C1)

Breeniome (C1)

Burrough (C1)

Bxz1 (C1)

Cactojaque (C1)

Cali (C1)

Cane17 (C1)

Catera (C1)

Chargie21 (C1)

CharlieB (C1)

ChickenPhender (C1)

Colt (C1)

Daffodil (C1)

Dandelion (C1)

Darko (C1)

Delilah (C1)

Derek (C1)

Dietrick (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Flabslab (C1)

Fludd (C1)

FoxtrotP1 (C1)

FrayBell (C1)

FudgeTart (C1)

Gabriel (C1)

Ghost (C1)

Gizmo (C1)

Grungle (C1)

Guwapp (C1)

HyRo (C1)

InigoMontoya (C1)

InterFolia (C1)

Iota (C1)

Jessibeth14 (C1)

JPickles (C1)

JustHall (C1)

Kamryn (C1)

Kboogie (C1)

Khaleesi (C1)

Koguma (C1)

LifeSavor (C1)

LinStu (C1)

Littleton (C1)

Loanshark (C1)

LolaVinca (C1)

LRRHood (C1)

Lukilu (C1)

Mangeria (C1)

McWolfish (C1)

Megamind (C1)

Melpomini (C1)

MikeLiesIn (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

Naija (C1)

Napoleon13 (C1)

Nappy (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

ParkTD (C1)

Patter (C1)

Phlegm (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pinkcreek (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

QBert (C1)

Rabinovish (C1)

Rahel (C1)

Rizal (C1)

RoMag (C1)

Roots515 (C1)

Salacia (C1)

Sauce (C1)

ScottMcG (C1)

Sebata (C1)

Shaqnato (C1)

Shelob (C1)

ShiaLabeouf (C1)

Shifa (C1)

Shnickers (C1)

Shrimp (C1)

SmallFry (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

StephanieG (C1)

Stubby (C1)

Teardrop (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Trinitium (C1)

Tyke (C1)

ValleyTerrace (C1)

Wally (C1)

Willis (C1)

YoungMoneyMata (C1)

Yucca (C1)

Zalkecks (C1)

Zeenon (C1)

ZygoTaiga (C1)

Myrna (C2)

Phabba (C2)

Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
Plaque NotesSubsequent to serial dilution, spot assays and streak tests, the putative plaque which was isolated and further refined through MTL & HTL protocols produced lytic plaques which were approximately 0.5 mm in diameter, consistent in morphology throughout the area of the agar plate and also consistent in opacity. The area of the individual plaques did not exceed the aforementioned measurement, and were robust in quantity.
MorphotypeMyoviridae
Has been Phamerated?Yes
Gene List

Click to View

Colt_1

Colt_2

Colt_3
(HNH endonuclease)

Colt_4

Colt_5

Colt_6

Colt_7

Colt_8

Colt_9

Colt_10

Colt_11

Colt_12

Colt_13

Colt_14

Colt_15

Colt_16

Colt_17

Colt_18

Colt_19

Colt_20

Colt_21

Colt_22

Colt_23

Colt_24
(helix-turn-helix DNA binding protein)

Colt_25
(HNH endonuclease)

Colt_26

Colt_27

Colt_28

Colt_29

Colt_30

Colt_31

Colt_32

Colt_33

Colt_34

Colt_35

Colt_36

Colt_37

Colt_38

Colt_39

Colt_40

Colt_41

Colt_42

Colt_43

Colt_44

Colt_45

Colt_46

Colt_47
(helix-turn-helix DNA binding protein)

Colt_48

Colt_49

Colt_50

Colt_51

Colt_52

Colt_53

Colt_54
(helix-turn-helix DNA binding domain protein)

Colt_55
(helix-turn-helix DNA binding domain protein)

Colt_56

Colt_57

Colt_58

Colt_59

Colt_60

Colt_61

Colt_62

Colt_63

Colt_64

Colt_65

Colt_66

Colt_67

Colt_68

Colt_69

Colt_70

Colt_71

Colt_72

Colt_73

Colt_78
(methyltransferase)

Colt_79

Colt_80
(portal protein)

Colt_81

Colt_82

Colt_83
(ThyX-like thymidylate synthase)

Colt_84

Colt_85
(RNA ligase)

Colt_86
(LysM domain protein)

Colt_87

Colt_88

Colt_89
(major capsid protein)

Colt_90
(holliday junction resolvase)

Colt_91
(capsid decoration protein)

Colt_92

Colt_93

Colt_94

Colt_95

Colt_96

Colt_97

Colt_98

Colt_99
(membrane protein)

Colt_100

Colt_101

Colt_102

Colt_103

Colt_104

Colt_105

Colt_106

Colt_107

Colt_108

Colt_109

Colt_110

Colt_111

Colt_112
(N-acetyltransferase)

Colt_113

Colt_114

Colt_115

Colt_116

Colt_117

Colt_118

Colt_119
(tail sheath protein)

Colt_120

Colt_121

Colt_122
(tail assembly chaperone)

Colt_123
(tail assembly chaperone)

Colt_124
(tape measure protein)

Colt_125
(minor tail protein)

Colt_126

Colt_127

Colt_128
(minor tail protein)

Colt_129

Colt_130

Colt_131
(baseplate wedge protein)

Colt_132
(baseplate wedge protein)

Colt_133

Colt_134

Colt_135

Colt_136
(minor tail protein)

Colt_137
(minor tail protein)

Colt_138

Colt_139

Colt_140

Colt_141

Colt_142

Colt_143

Colt_144

Colt_148

Colt_149
(peptidyl tRNA transferase)

Colt_164
(D-ala-D-ala carboxypeptidase)

Colt_165

Colt_177

Colt_178

Colt_179
(helicase)

Colt_180

Colt_181

Colt_182

Colt_183

Colt_184

Colt_185

Colt_186

Colt_187

Colt_188

Colt_189
(thymidylate kinase)

Colt_190
(aminotransferase)

Colt_191
(RNA binding domain protein)

Colt_192

Colt_193

Colt_194
(DnaC-like helicase loader)

Colt_195
(DnaB-like dsDNA helicase)

Colt_196
(DNA primase)

Colt_197

Colt_198
(DnaJ-like chaperonin)

Colt_199
(ssDNA binding domain protein)

Colt_200
(DNA polymerase III (alpha))

Colt_201
(RF-1 peptide chain release factor)

Colt_202
(RecA-like DNA recombinase)

Colt_203
(holliday junction resolvase)

Colt_204

Colt_205
(HNH endonuclease)

Colt_206
(RusA-like resolvase)

Colt_207

Colt_208

Colt_209

Colt_210

Colt_211
(glycoside hydrolase)

Colt_212

Colt_213

Colt_214

Colt_217

Colt_218

Colt_219

Colt_220

Colt_221

Colt_222

Colt_223

Colt_224
(Ro-like RNA binding protein)

Colt_225

Colt_226

Colt_227

Colt_228

Colt_229

Colt_230
(galactosaminyltransferase)

Colt_231

Colt_232
(glycosyltransferase)

Colt_233
(galactosyltransferase)

Colt_234
(nicotinate ribosyltransferase)

Colt_235
(glycosyltransferase)

Colt_236

Colt_237

Colt_238

Colt_239

Colt_240
(lysin A)

Colt_241
(membrane protein)

Colt_242
(lysin B)

Colt_243
(HNH endonuclease)

Colt_244
(terminase)

Colt_245

Colt_246

Colt_247

Colt_248

Colt_249
(polynucleotide kinase)

Colt_250

Colt_251
(serine/threonine kinase)

Colt_252

Colt_253
(adenylosuccinate synthetase)

Colt_254

Colt_255

Colt_256

Colt_257

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK359359
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Date of SEA Lysate Titering Nov 9, 2015
Pitt Freezer Box# 23
Pitt Freezer Box Grid# H11
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload