Mycobacterium phage CindyLou
Know something about this phage that we don't? Modify its data.
Detailed Information for Phage CindyLou
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByAlexa Mihaita
Year Found2021
Location FoundSpringfield, PA United States of America
Finding InstitutionSaint Joseph's University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature-1°C
GPS Coordinates39.950167 N, 75.334002 W Map
Discovery NotesThe soil sample containing phage CindyLou was collected on January 23rd at 10:51 AM from beneath an evergreen tree next to the deck at Alexa's home in Springfield, Pennsylvania. The soil was recovered from a depth of 45.72 cm and was moist. The ambient humidity was 34%, and the weather was cold, dry, and mostly cloudy with 21 mph gusts of wind. The HTL titer was 6.8 x 10^9 pfu/ml. The DNA concentration was 419.2 ng/ul. Based on the electron micrograph, CindyLou's average capsid diameter was 68.6 nm, and the average tail length was 92.9 nm (n=3). A PCR test using C1-specific primers strongly amplified CindyLou's DNA, providing evidence that CindyLou may belong to the C1 cluster.
Naming NotesThe phage was named CindyLou after the very tiny Dr. Seuss character, since the phage always yielded very small plaques from 0.5 mm - 1 mm in diameter.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 9, 2021
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage68
Genome length (bp)157677
Character of genome endsCircularly Permuted
GC Content64.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Cluster Life CycleLytic
Other Cluster Members

Click to View

Ading (C1)

Adlitam (C1)

Alice (C1)

Amataga (C1)

ArcherS7 (C1)

Astraea (C1)

Atlantean (C1)

Audrick (C1)

Ava3 (C1)

Babyland (C1)

BackyardAgain (C1)

BadAgartude (C1)

BananaFence (C1)

Bangla1971 (C1)

Basquiat (C1)

BeanWater (C1)

Bigswole (C1)

Bipolarisk (C1)

Blackbrain (C1)

Bonray (C1)

Bread (C1)

Breeniome (C1)

Burrough (C1)

Bxz1 (C1)

Cactojaque (C1)

Cali (C1)

Cane17 (C1)

Capablanca (C1)

Catera (C1)

Chargie21 (C1)

CharlieB (C1)

ChaylaJr (C1)

ChickenPhender (C1)

Chomp (C1)

CindyLou (C1)

Colt (C1)

Daffodil (C1)

Dandelion (C1)

Darko (C1)

Delilah (C1)

Delylah (C1)

Derek (C1)

Dietrick (C1)

DirtMonster (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EasyJones (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Flabslab (C1)

Fludd (C1)

FoxtrotP1 (C1)

FrayBell (C1)

FudgeTart (C1)

Gabriel (C1)

Ghost (C1)

Gizmo (C1)

Grungle (C1)

Guwapp (C1)

HyRo (C1)

I3 (C1)

IkeLoa (C1)

InigoMontoya (C1)

InterFolia (C1)

Iota (C1)

Izajani (C1)

Janiyra (C1)

Jaygup (C1)

Jedediah (C1)

Jemmno (C1)

Jessibeth14 (C1)

JPickles (C1)

JustHall (C1)

Kamryn (C1)

Kboogie (C1)

Khaleesi (C1)

Koguma (C1)

Latch (C1)

LifeSavor (C1)

LinStu (C1)

Littleton (C1)

Loanshark (C1)

LolaVinca (C1)

LRRHood (C1)

Lukilu (C1)

Maby2021 (C1)

Mangeria (C1)

McGee (C1)

McWolfish (C1)

Megamind (C1)

Melpomini (C1)

MikeLiesIn (C1)

Mikro (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

Naija (C1)

Napoleon13 (C1)

Nappy (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

ParkTD (C1)

Patter (C1)

Phlegm (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pinkcreek (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

QBert (C1)

Quasimodo (C1)

Rabinovish (C1)

Rahel (C1)

Rizal (C1)

RoMag (C1)

Ronan (C1)

Roots515 (C1)

Salacia (C1)

Sauce (C1)

ScottMcG (C1)

Sebata (C1)

Shaqnato (C1)

Shelob (C1)

ShiaLabeouf (C1)

Shifa (C1)

Shnickers (C1)

Shrimp (C1)

SilverDipper (C1)

SmallFry (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

StephanieG (C1)

Stubby (C1)

Teardrop (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Trinitium (C1)

Turret (C1)

Tyke (C1)

ValleyTerrace (C1)

Wally (C1)

Willis (C1)

Yassified (C1)

YemiJoy2021 (C1)

YoungMoneyMata (C1)

Yucca (C1)

Zalkecks (C1)

Zeenon (C1)

ZygoTaiga (C1)

Myrna (C2)

Phabba (C2)

ScoobyDoobyDoo (C2)

Annotating InstitutionSaint Joseph's University
Annotation StatusIn GenBank
Plaque NotesPlaques are circular and clear, with an average diameter of about 0.8 mm after a 24 hour incubation period.
MorphotypeMyoviridae
Has been Phamerated?Yes
Gene List

Click to View

CindyLou_1

CindyLou_2

CindyLou_3
(nucleotidyl transferase)

CindyLou_4

CindyLou_5

CindyLou_6

CindyLou_7

CindyLou_8

CindyLou_9

CindyLou_10

CindyLou_11

CindyLou_12

CindyLou_13

CindyLou_14

CindyLou_15

CindyLou_16

CindyLou_17
(histidine triad nucleotide binding protein)

CindyLou_18

CindyLou_19

CindyLou_20

CindyLou_21

CindyLou_22

CindyLou_23

CindyLou_24

CindyLou_25
(HNH endonuclease)

CindyLou_26

CindyLou_27

CindyLou_28

CindyLou_29
(band-7-like membrane protein)

CindyLou_30

CindyLou_31

CindyLou_32

CindyLou_33

CindyLou_34

CindyLou_35
(ribbon-helix-helix DNA binding domain protein)

CindyLou_36

CindyLou_37

CindyLou_38

CindyLou_39

CindyLou_40

CindyLou_41

CindyLou_42

CindyLou_43

CindyLou_44

CindyLou_45

CindyLou_46

CindyLou_47

CindyLou_48

CindyLou_49

CindyLou_50

CindyLou_51

CindyLou_52

CindyLou_53

CindyLou_54

CindyLou_55

CindyLou_56

CindyLou_57

CindyLou_58

CindyLou_59

CindyLou_60

CindyLou_61

CindyLou_62

CindyLou_63
(helix-turn-helix DNA binding domain protein)

CindyLou_64
(helix-turn-helix DNA binding domain protein)

CindyLou_65

CindyLou_66

CindyLou_67

CindyLou_68

CindyLou_69

CindyLou_70

CindyLou_71

CindyLou_72

CindyLou_73

CindyLou_74

CindyLou_75

CindyLou_76

CindyLou_77

CindyLou_78

CindyLou_79

CindyLou_80

CindyLou_81

CindyLou_82

CindyLou_83

CindyLou_84
(ParB-like nuclease domain protein)

CindyLou_89

CindyLou_90

CindyLou_91
(portal protein)

CindyLou_92

CindyLou_93

CindyLou_94
(ThyX-like thymidylate synthase)

CindyLou_95

CindyLou_96
(RNA ligase)

CindyLou_97
(HNH endonuclease)

CindyLou_98

CindyLou_99

CindyLou_100

CindyLou_101

CindyLou_102

CindyLou_103

CindyLou_104

CindyLou_105

CindyLou_106

CindyLou_107

CindyLou_108

CindyLou_109

CindyLou_110

CindyLou_111

CindyLou_112

CindyLou_113

CindyLou_114

CindyLou_115

CindyLou_116

CindyLou_117

CindyLou_118

CindyLou_119

CindyLou_120

CindyLou_121

CindyLou_122

CindyLou_123

CindyLou_124

CindyLou_125

CindyLou_126

CindyLou_127

CindyLou_128

CindyLou_129
(tail sheath protein)

CindyLou_130

CindyLou_131

CindyLou_132
(tail assembly chaperone)

CindyLou_133
(tail assembly chaperone)

CindyLou_134
(tape measure protein)

CindyLou_135
(minor tail protein)

CindyLou_136

CindyLou_137

CindyLou_138
(minor tail protein)

CindyLou_139

CindyLou_140
(minor tail protein)

CindyLou_141
(baseplate wedge protein)

CindyLou_142
(baseplate J protein)

CindyLou_143

CindyLou_144

CindyLou_145

CindyLou_146
(minor tail protein)

CindyLou_147
(minor tail protein)

CindyLou_148

CindyLou_149

CindyLou_150

CindyLou_151

CindyLou_152

CindyLou_153

CindyLou_154

CindyLou_155

CindyLou_159

CindyLou_160
(peptidyl tRNA hydrolase)

CindyLou_174
(HNH endonuclease)

CindyLou_176
(minor tail protein)

CindyLou_177

CindyLou_188

CindyLou_189
(DNA helicase)

CindyLou_190

CindyLou_191

CindyLou_192

CindyLou_193

CindyLou_194

CindyLou_195

CindyLou_196

CindyLou_197

CindyLou_198
(thymidylate kinase)

CindyLou_199
(aminotransferase)

CindyLou_200

CindyLou_201

CindyLou_202

CindyLou_203
(DnaC-like helicase loader)

CindyLou_204
(DnaB-like dsDNA helicase)

CindyLou_205

CindyLou_206
(DNA primase)

CindyLou_207

CindyLou_208
(DnaJ-like chaperonin)

CindyLou_209
(ssDNA binding protein)

CindyLou_210
(DnaE-like DNA polymerase III alpha)

CindyLou_211
(RF-1 peptide chain release factor)

CindyLou_212
(RecA-like DNA recombinase)

CindyLou_213
(holliday junction resolvase)

CindyLou_214

CindyLou_215
(HNH endonuclease)

CindyLou_216
(RusA-like resolvase)

CindyLou_217

CindyLou_218

CindyLou_219

CindyLou_220

CindyLou_221

CindyLou_222

CindyLou_223

CindyLou_226

CindyLou_227

CindyLou_228

CindyLou_229

CindyLou_230

CindyLou_231

CindyLou_232

CindyLou_233

CindyLou_234
(Ro-like RNA binding protein)

CindyLou_235

CindyLou_236

CindyLou_237

CindyLou_238

CindyLou_239

CindyLou_240

CindyLou_241
(glycosyltransferase)

CindyLou_242

CindyLou_243
(glycosyltransferase)

CindyLou_244
(glycosyltransferase)

CindyLou_245
(PnuC-like nicotinamide riboside transporter)

CindyLou_246
(glycosyltransferase)

CindyLou_247

CindyLou_248

CindyLou_249

CindyLou_250

CindyLou_251
(lysin A)

CindyLou_252
(holin)

CindyLou_253
(lysin B)

CindyLou_254
(terminase)

CindyLou_255

CindyLou_256

CindyLou_257

CindyLou_258

CindyLou_259
(polynucleotide kinase)

CindyLou_260

CindyLou_261
(serine/threonine kinase)

CindyLou_262

CindyLou_263

CindyLou_264
(PurA-like adenylosuccinate synthetase)

CindyLou_265

CindyLou_266

CindyLou_267

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionON081335
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 129
Pitt Freezer Box Grid# H6
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload