Mycobacterium phage JPickles
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Detailed Information for Phage JPickles
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByLeya Givvines
Year Found2018
Location FoundPhiladelphia, PA USA
Finding InstitutionSaint Joseph's University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates39.99479 N, 75.243011 W Map
Discovery Notes-Collected at 11:14 am on September 4th 2018
-Collected near Saint Joseph's University Chapel
-Soil Slightly moist at collection
-Soil was collected 5.1 cm down
-Soil collected while outside temperature was 32.78 degrees Celsius
-Humidity was 50% at time of collection
-There was moderate temperature days before collection.
Naming NotesPickles is a code word that my boyfriend and I use and his name starts with a J, therefore JPickles. Name was changed from Pickles1025 to JPickles after second titration for simplicity.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 25, 2019
Sequencing FacilityNC State Genomic Sciences Laboratory
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1221
Genome length (bp)155116
Character of genome endsCircularly Permuted
GC Content64.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Cluster Life CycleLytic
Other Cluster Members

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Ading (C1)

Adlitam (C1)

Alice (C1)

Amataga (C1)

ArcherS7 (C1)

Astraea (C1)

Atlantean (C1)

Audrick (C1)

Ava3 (C1)

BadAgartude (C1)

Bangla1971 (C1)

Basquiat (C1)

BeanWater (C1)

Bigswole (C1)

Bipolarisk (C1)

Blackbrain (C1)

Bonray (C1)

Bread (C1)

Breeniome (C1)

Burrough (C1)

Bxz1 (C1)

Cactojaque (C1)

Cali (C1)

Cane17 (C1)

Catera (C1)

Chargie21 (C1)

CharlieB (C1)

ChickenPhender (C1)

Colt (C1)

Daffodil (C1)

Dandelion (C1)

Darko (C1)

Delilah (C1)

Derek (C1)

Dietrick (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Flabslab (C1)

Fludd (C1)

FoxtrotP1 (C1)

FrayBell (C1)

FudgeTart (C1)

Gabriel (C1)

Ghost (C1)

Gizmo (C1)

Grungle (C1)

Guwapp (C1)

HyRo (C1)

InigoMontoya (C1)

InterFolia (C1)

Iota (C1)

Jessibeth14 (C1)

JPickles (C1)

JustHall (C1)

Kamryn (C1)

Kboogie (C1)

Khaleesi (C1)

Koguma (C1)

LifeSavor (C1)

LinStu (C1)

Littleton (C1)

Loanshark (C1)

LolaVinca (C1)

LRRHood (C1)

Lukilu (C1)

Mangeria (C1)

McWolfish (C1)

Megamind (C1)

Melpomini (C1)

MikeLiesIn (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

Naija (C1)

Napoleon13 (C1)

Nappy (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

ParkTD (C1)

Patter (C1)

Phlegm (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pinkcreek (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

QBert (C1)

Rabinovish (C1)

Rahel (C1)

Rizal (C1)

RoMag (C1)

Roots515 (C1)

Salacia (C1)

Sauce (C1)

ScottMcG (C1)

Sebata (C1)

Shaqnato (C1)

Shelob (C1)

ShiaLabeouf (C1)

Shifa (C1)

Shnickers (C1)

Shrimp (C1)

SmallFry (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

StephanieG (C1)

Stubby (C1)

Teardrop (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Trinitium (C1)

Tyke (C1)

ValleyTerrace (C1)

Wally (C1)

Willis (C1)

YoungMoneyMata (C1)

Yucca (C1)

Zalkecks (C1)

Zeenon (C1)

ZygoTaiga (C1)

Myrna (C2)

Phabba (C2)

Annotating InstitutionSaint Joseph's University
Annotation StatusQC Complete
Plaque NotesPhage produced clear plaques ranging from 2 to 4 mm in diameter. Plaques developed after two days in incubator at 37 degrees Celsius.
MorphotypeMyoviridae
Has been Phamerated?Yes
Gene List

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JPickles_1

JPickles_2

JPickles_3
(Nucleotidyl transferase)

JPickles_4

JPickles_5

JPickles_6

JPickles_7

JPickles_8

JPickles_9
(membrane protein)

JPickles_10

JPickles_11

JPickles_12

JPickles_13

JPickles_14

JPickles_15

JPickles_16

JPickles_17
(histidine triad nucleotide binding protein)

JPickles_18

JPickles_19

JPickles_20

JPickles_21

JPickles_22

JPickles_23

JPickles_24

JPickles_25

JPickles_26

JPickles_27
(membrane protein, Band-7 -like)

JPickles_28

JPickles_29
(helix-turn-helix DNA binding domain)

JPickles_30

JPickles_31

JPickles_32

JPickles_33
(ribbon-helix-helix DNA binding domain)

JPickles_34

JPickles_35

JPickles_36

JPickles_37

JPickles_38

JPickles_39

JPickles_40

JPickles_41

JPickles_42

JPickles_43

JPickles_44

JPickles_45

JPickles_46

JPickles_47

JPickles_48

JPickles_49

JPickles_50

JPickles_51

JPickles_52

JPickles_53
(helix-turn-helix DNA binding domain)

JPickles_54

JPickles_55

JPickles_56

JPickles_57

JPickles_58

JPickles_59

JPickles_60
(helix-turn-helix DNA binding domain)

JPickles_61
(helix-turn-helix DNA binding domain)

JPickles_62

JPickles_63

JPickles_64

JPickles_65

JPickles_66

JPickles_67

JPickles_68

JPickles_69

JPickles_70

JPickles_71

JPickles_72

JPickles_73

JPickles_74

JPickles_75

JPickles_76

JPickles_77

JPickles_78

JPickles_79

JPickles_80

JPickles_81
(ParB-like nuclease domain)

JPickles_86
(methyltransferase)

JPickles_87

JPickles_88
(portal protein)

JPickles_89

JPickles_90

JPickles_91
(ThyX-like thymidylate synthase)

JPickles_92

JPickles_93
(RNA ligase)

JPickles_94
(HNH endonuclease)

JPickles_95
(endolysin, LysM-like)

JPickles_96

JPickles_97

JPickles_98
(major capsid protein)

JPickles_99
(capsid decoration protein)

JPickles_100

JPickles_101

JPickles_102

JPickles_103

JPickles_104

JPickles_105

JPickles_106
(membrane protein)

JPickles_107

JPickles_108

JPickles_109

JPickles_110

JPickles_111

JPickles_112
(capsid decoration protein)

JPickles_113

JPickles_114

JPickles_115

JPickles_116

JPickles_117

JPickles_118
(acetyltransferase)

JPickles_119

JPickles_120

JPickles_121

JPickles_122

JPickles_123

JPickles_124
(tail sheath protein)

JPickles_125

JPickles_126

JPickles_127
(tail assembly chaperone)

JPickles_128
(tail assembly chaperone)

JPickles_129
(tape measure protein)

JPickles_130

JPickles_131

JPickles_132

JPickles_133
(minor tail protein)

JPickles_134

JPickles_135
(minor tail protein)

JPickles_136
(baseplate wedge protein)

JPickles_137
(baseplate J protein)

JPickles_138
(HNH endonuclease)

JPickles_139

JPickles_140

JPickles_141
(minor tail protein)

JPickles_142
(minor tail protein)

JPickles_143

JPickles_144

JPickles_145

JPickles_146

JPickles_147

JPickles_148

JPickles_149

JPickles_153

JPickles_154
(peptidyl tRNA hydrolase)

JPickles_160

JPickles_170
(minor tail protein, D-ala-D-ala carboxypeptidase)

JPickles_171

JPickles_183

JPickles_184
(helicase)

JPickles_185

JPickles_186

JPickles_187

JPickles_188

JPickles_189

JPickles_190

JPickles_191

JPickles_192

JPickles_193
(thymidylate kinase)

JPickles_194
(aminotransferase)

JPickles_195

JPickles_196

JPickles_197

JPickles_198
(DnaC-like helicase loader)

JPickles_199
(DnaB-like dsDNA helicase)

JPickles_200

JPickles_201
(DNA primase)

JPickles_202

JPickles_203
(chaperonin, DnaJ-like)

JPickles_204

JPickles_205
(DnaE-like DNA polymerase III (alpha))

JPickles_206
(RF-1 peptide chain release factor)

JPickles_207
(RecA-like DNA recombinase)

JPickles_208
(Holliday junction resolvase)

JPickles_209

JPickles_210
(HNH endonuclease)

JPickles_211
(RusA-like resolvase (endonuclease))

JPickles_212

JPickles_213

JPickles_214

JPickles_215

JPickles_216

JPickles_217

JPickles_218
(HNH endonuclease)

JPickles_219

JPickles_222

JPickles_223

JPickles_224

JPickles_225

JPickles_226
(membrane protein)

JPickles_227

JPickles_228

JPickles_229

JPickles_230
(Ro-like RNA binding protein)

JPickles_231

JPickles_232

JPickles_233

JPickles_234

JPickles_235

JPickles_236

JPickles_237
(glycosyltransferase)

JPickles_238
(membrane protein)

JPickles_239
(glycosyltransferase)

JPickles_240
(glycosyltransferase)

JPickles_241
(membrane protein)

JPickles_242
(glycosyltransferase)

JPickles_243

JPickles_244

JPickles_245

JPickles_246

JPickles_247
(HNH endonuclease)

JPickles_248
(lysin A)

JPickles_249
(holin)

JPickles_250
(lysin B)

JPickles_251
(terminase)

JPickles_252

JPickles_253

JPickles_254

JPickles_255

JPickles_256
(polynucleotide kinase)

JPickles_257

JPickles_258
(serine/threonine kinase)

JPickles_259

JPickles_260

JPickles_261
(adenylosuccinate synthetase, PurA-like)

JPickles_262

JPickles_263

JPickles_264

JPickles_265

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 85
Pitt Freezer Box Grid# D12
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload