The Actinobacteriophage Database

Mycobacterium phage QBert

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Detailed Information for Phage QBert
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Hannah Marie Youngwirth
Year Found	2012
Location Found	La Jolla, CA USA
Finding Institution	University of California, San Diego
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	Not entered
GPS Coordinates	32.8 N, 117.2 W Map
Discovery Notes	10/2/2012, 8:30 am Compost Soil, Approx. 2” deep Temp: 80° F, 40% Humidity La Jolla, CA Moist compost early in decomp process, large zucchini leaves and other plant pieces still intact mixed among woodchips, soil was warm which reveals the process of decomp, in contact with ground soil, open system
Naming Notes	Named from a poll I ran with my family-one coined the name QBert from the 70's video game, because the shape of the main character and my phage are very similar.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jan 28, 2013
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Ion Torrent Sequencing
Approximate Shotgun Coverage	320
Sanger Finishing Reactions	6
Genome length (bp)	153772
Character of genome ends	Circularly Permuted
GC Content	64.8%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	C
Subcluster	C1
Cluster Life Cycle	Lytic
Other Cluster Members	Click to View Ading (C1) Adlitam (C1) Alice (C1) Amataga (C1) ArcherS7 (C1) Asterius (C1) Astraea (C1) Atlantean (C1) Audrick (C1) Ava3 (C1) Babyland (C1) BackyardAgain (C1) BadAgartude (C1) BananaFence (C1) Bangla1971 (C1) Basquiat (C1) BeanWater (C1) BigCity (C1) Bigswole (C1) Bipolarisk (C1) Blackbrain (C1) Blubba (C1) Bonray (C1) Bread (C1) Breeniome (C1) Burrough (C1) Bxz1 (C1) Cactojaque (C1) Cali (C1) Cane17 (C1) Capablanca (C1) Caravan (C1) Catera (C1) Chargie21 (C1) CharlieB (C1) ChaylaJr (C1) ChickenPhender (C1) Chomp (C1) CindyLou (C1) Colt (C1) Concombre (C1) Daffodil (C1) Dandelion (C1) Darko (C1) Delilah (C1) Delylah (C1) Derek (C1) Dietrick (C1) DirtMonster (C1) Drazdys (C1) DrPhinkDaddy (C1) DTDevon (C1) EasyJones (C1) EggyFarm (C1) EmmaElysia (C1) EmToTheThree (C1) Erdmann (C1) ErnieJ (C1) Essence (C1) ET08 (C1) Ewok (C1) Flabslab (C1) Fludd (C1) FoxtrotP1 (C1) FrayBell (C1) FudgeTart (C1) Gabriel (C1) Gamgam (C1) Ghost (C1) Gizmo (C1) Grasshills (C1) Grungle (C1) Guwapp (C1) Halldule (C1) HyRo (C1) I3 (C1) IkeLoa (C1) InigoMontoya (C1) InterFolia (C1) Iota (C1) Issimir (C1) Izajani (C1) Janiyra (C1) Jaygup (C1) JayJay (C1) Jedediah (C1) Jemmno (C1) Jessibeth14 (C1) JPickles (C1) JulietS (C1) JustHall (C1) Kamryn (C1) Kboogie (C1) Khaleesi (C1) Koguma (C1) Latch (C1) Lee7 (C1) LifeSavor (C1) LinStu (C1) Littleton (C1) Loanshark (C1) LolaVinca (C1) LordLeafolot (C1) LRRHood (C1) Lukilu (C1) Maby2021 (C1) Mangeria (C1) Matsumoto (C1) McGee (C1) McWolfish (C1) Megamind (C1) Melpomini (C1) MikeLiesIn (C1) Mikro (C1) Momo (C1) MoMoMixon (C1) Morizzled23 (C1) Naija (C1) Napoleon13 (C1) Nappy (C1) Naval22 (C1) NedWong (C1) Ngannou (C1) Nidhogg (C1) NoodleTree (C1) Norm (C1) NuevoMundo (C1) ParkTD (C1) Patter (C1) Phlegm (C1) Phox (C1) Phusco (C1) Pier (C1) Pinkcreek (C1) Pio (C1) Pivoine (C1) Pleione (C1) PurduePete (C1) QBert (C1) Quasimodo (C1) Rabinovish (C1) Rahel (C1) Rizal (C1) RoMag (C1) Ronan (C1) Roots515 (C1) Salacia (C1) Sauce (C1) ScottMcG (C1) Sebata (C1) Shaqnato (C1) Shelob (C1) ShiaLabeouf (C1) Shifa (C1) Shnickers (C1) Shrimp (C1) SilverDipper (C1) SmallFry (C1) Spec (C1) Specks (C1) Sprinklers (C1) Spud (C1) SrishMeg2525 (C1) StephanieG (C1) Stubby (C1) StubbySaigey (C1) Teardrop (C1) TinyTim (C1) Tonenili (C1) Tortoise16 (C1) Trinitium (C1) Turret (C1) Tyke (C1) ValleyTerrace (C1) Wally (C1) Willis (C1) Yassified (C1) YemiJoy2021 (C1) YoungMoneyMata (C1) Yucca (C1) Zalkecks (C1) Zeenon (C1) ZygoTaiga (C1) Greely (C2) Myrna (C2) Phabba (C2) ScoobyDoobyDoo (C2)
Annotating Institution	University of Pittsburgh
Annotation Status	In GenBank
Plaque Notes	Plaques were generally around 1mm in diameter after 24 hours of incubation at 37°, after 48 hours some reached 3mm maximum, plaques were clear (lytic) Titer of 1.6E11
Morphotype	Myoviridae
Has been Phamerated?	Yes
Gene List	Click to View QBert_1 QBert_2 QBert_3 (HNH endonuclease) QBert_4 QBert_5 QBert_6 QBert_7 QBert_8 QBert_9 QBert_10 QBert_11 QBert_12 QBert_13 QBert_14 QBert_15 QBert_16 QBert_17 QBert_18 QBert_19 QBert_20 QBert_21 QBert_22 QBert_23 QBert_24 QBert_25 QBert_26 QBert_27 QBert_28 QBert_29 QBert_30 QBert_31 QBert_32 QBert_33 (ribbon-helix-helix DNA binding protein) QBert_34 QBert_35 QBert_36 QBert_37 QBert_38 QBert_39 QBert_40 QBert_41 QBert_42 QBert_43 QBert_44 QBert_45 QBert_46 QBert_47 QBert_48 QBert_49 QBert_50 QBert_51 QBert_52 QBert_53 QBert_54 QBert_55 QBert_56 QBert_57 (helix-turn-helix DNA binding domain protein) QBert_58 (helix-turn-helix DNA binding protein) QBert_59 QBert_60 QBert_61 QBert_62 QBert_63 QBert_64 QBert_65 QBert_66 QBert_67 QBert_68 QBert_69 QBert_70 QBert_71 QBert_72 QBert_73 QBert_74 QBert_75 QBert_76 QBert_77 QBert_82 QBert_83 QBert_84 (portal protein) QBert_85 QBert_86 QBert_87 (ThyX-like thymidylate synthase) QBert_88 QBert_89 (RNA ligase) QBert_90 QBert_91 QBert_92 QBert_93 (major capsid protein) QBert_94 (holliday junction resolvase) QBert_95 QBert_96 QBert_97 QBert_98 QBert_99 QBert_100 QBert_101 QBert_102 QBert_103 QBert_104 QBert_105 QBert_106 QBert_107 QBert_108 QBert_109 QBert_110 QBert_111 QBert_112 QBert_113 QBert_114 QBert_115 (acetyltransferase) QBert_116 QBert_117 QBert_118 QBert_119 QBert_120 QBert_121 (tail sheath protein) QBert_122 QBert_123 QBert_124 (tail assembly chaperone) QBert_125 (tail assembly chaperone) QBert_126 (tape measure protein) QBert_127 (minor tail protein) QBert_128 QBert_129 QBert_130 (minor tail protein) QBert_131 QBert_132 QBert_133 (baseplate wedge protein) QBert_134 (baseplate wedge protein) QBert_135 QBert_136 QBert_137 QBert_138 (minor tail protein) QBert_139 (minor tail protein) QBert_140 QBert_141 QBert_142 QBert_143 QBert_144 QBert_145 QBert_146 QBert_149 QBert_150 (peptidyl tRNA hydrolase) QBert_165 (minor tail protein) QBert_166 QBert_178 QBert_179 (DNA helicase) QBert_180 QBert_181 QBert_182 QBert_183 QBert_184 QBert_185 QBert_186 QBert_187 QBert_188 QBert_189 (thymidylate kinase) QBert_190 (aminotransferase) QBert_191 QBert_192 QBert_193 QBert_194 (DnaC-like helicase loader) QBert_195 (DnaB-like dsDNA helicase) QBert_196 QBert_197 (DNA primase) QBert_198 QBert_199 (DnaJ-like chaperonin) QBert_200 (ssDNA binding protein) QBert_201 (DnaE-like DNA polymerase III) QBert_202 (RF-1 peptide chain release factor) QBert_203 (RecA-like DNA recombinase) QBert_204 (holliday junction resolvase) QBert_205 QBert_206 (HNH endonuclease) QBert_207 (RusA-like resolvase) QBert_208 QBert_209 QBert_210 QBert_211 QBert_212 QBert_213 QBert_214 QBert_217 QBert_218 QBert_219 QBert_220 QBert_221 QBert_222 QBert_223 QBert_224 QBert_225 (Ro-like RNA binding protein) QBert_226 QBert_227 QBert_228 QBert_229 QBert_230 QBert_231 QBert_232 (glycosyltransferase) QBert_233 QBert_234 (glycosyltransferase) QBert_235 (glycosyltransferase) QBert_236 (PnuC-like nicotinamide riboside transporter) QBert_237 (glycosyltransferase) QBert_238 QBert_239 QBert_240 QBert_241 QBert_242 (lysin A) QBert_243 QBert_244 (lysin B) QBert_245 (terminase) QBert_246 QBert_247 QBert_248 QBert_249 QBert_250 (polynucleotide kinase) QBert_251 QBert_252 QBert_253 (serine/threonine kinase) QBert_254 QBert_255 (PurA-like adenylosuccinate synthetase) QBert_256 QBert_257 QBert_258
Submitted Minimal DNA Master File	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MK359345
Refseq Number	None yet
Published in Paper	Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status	Not in Pitt Archives
SEA Lysate Titer	1.6E11
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
Final DNAMaster File	Download
GenBank File for Phamerator	Download

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