Mycobacterium phage QBert
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Detailed Information for Phage QBert
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByHannah Marie Youngwirth
Year Found2012
Location FoundLa Jolla, CA United States
Finding InstitutionUniversity of California, San Diego
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates32.8 N, 117.2 W Map
Discovery Notes10/2/2012, 8:30 am
Compost Soil, Approx. 2” deep
Temp: 80° F, 40% Humidity
La Jolla, CA
Moist compost early in decomp process, large zucchini leaves and other plant pieces still intact mixed among woodchips, soil was warm which reveals the process of decomp, in contact with ground soil, open system
Naming NotesNamed from a poll I ran with my family-one coined the name QBert from the 70's video game, because the shape of the main character and my phage are very similar.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 28, 2013
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIon Torrent Sequencing
Approximate Shotgun Coverage320
Sanger Finishing Reactions6
Genome length (bp)153772
Character of genome endsCircularly Permuted
GC Content64.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Cluster Life CycleLytic
Other Cluster Members

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Boonesok (None)

CLach (None)

DavidMina (None)

DitchDweller (None)

Elpis (None)

Genet (None)

Hard2get (None)

Hippocrates (None)

Kali (None)

Linnaeus (None)

LittleSpot (None)

Lsquared (None)

Marlex (None)

Minipoke (None)

MoBama (None)

PeeWee (None)

Pesky7 (None)

Phomnie (None)

Phyllis (None)

Roku (None)

Sagamore (None)

Samarai (None)

ShenziUR (None)

SuperCallie (None)

TinyC (None)

Trilla23 (None)

Turret (None)

RetimsGems (A1)

Squidney (A3)

Ading (C1)

Adlitam (C1)

Alice (C1)

Amataga (C1)

ArcherS7 (C1)

Astraea (C1)

Atlantean (C1)

Atlas (C1)

Audrick (C1)

Ava3 (C1)

BadAgartude (C1)

Bangla1971 (C1)

Basquiat (C1)

BeanWater (C1)

Bigswole (C1)

Bipolarisk (C1)

Blackbrain (C1)

Bonray (C1)

Bread (C1)

Breeniome (C1)

Burrough (C1)

Bxz1 (C1)

Cactojaque (C1)

Cali (C1)

Cane17 (C1)

Catera (C1)

Chargie21 (C1)

CharlieB (C1)

ChickenPhender (C1)

Colt (C1)

Daffodil (C1)

Dandelion (C1)

Darko (C1)

Delilah (C1)

Derek (C1)

Dietrick (C1)

Donegal (C1)

Doodle1 (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Exxus285 (C1)

Flabslab (C1)

Fludd (C1)

FoxtrotP1 (C1)

FrayBell (C1)

FudgeTart (C1)

Gabriel (C1)

Ghost (C1)

Gizmo (C1)

Grungle (C1)

Guwapp (C1)

HarrySpotter (C1)

HyRo (C1)

InigoMontoya (C1)

InterFolia (C1)

Iota (C1)

Isee1101 (C1)

JennanaJr (C1)

Jessibeth14 (C1)

JustHall (C1)

Kamryn (C1)

Kboogie (C1)

Khaleesi (C1)

Koguma (C1)

LifeSavor (C1)

LinStu (C1)

Lionheart (C1)

Littleton (C1)

Loanshark (C1)

LolaVinca (C1)

LRRHood (C1)

Lukilu (C1)

McWolfish (C1)

Megamind (C1)

Melpomini (C1)

MePac (C1)

MikeLiesIn (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

MrsHay (C1)

Naija (C1)

Napoleon13 (C1)

Nappy (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

OaklaforG (C1)

ParkTD (C1)

Patch (C1)

Patter (C1)

Phlegm (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

QBert (C1)

Rabinovish (C1)

Rahel (C1)

Rizal (C1)

RoMag (C1)

Roots515 (C1)

Salacia (C1)

SandySandal (C1)

Sauce (C1)

ScottMcG (C1)

Sebastian (C1)

Sebata (C1)

ShankPowerGod (C1)

Shaqnato (C1)

Shelob (C1)

ShiaLabeouf (C1)

Shnickers (C1)

Shrimp (C1)

SmallFry (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

Stubby (C1)

Teardrop (C1)

TheO (C1)

Thuathua (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Trinitium (C1)

Tyke (C1)

ValleyTerrace (C1)

Wally (C1)

Willis (C1)

YoungMoneyMata (C1)

Yucca (C1)

Zalkecks (C1)

Zeenon (C1)

ZygoTaiga (C1)

Feauphix (C2)

Matcha (C2)

Myrna (C2)

Phabba (C2)

SimSimmers (C2)

SrishMeg2525 (C2)

Annotation StatusIn GenBank
Plaque NotesPlaques were generally around 1mm in diameter after 24 hours of incubation at 37°, after 48 hours some reached 3mm maximum, plaques were clear (lytic)
Titer of 1.6E11
MorphotypeMyoviridae
Has been Phamerated?Yes
Gene List

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QBert_1

QBert_2

QBert_3
(HNH endonuclease)

QBert_4

QBert_5

QBert_6

QBert_7

QBert_8

QBert_9

QBert_10

QBert_11

QBert_12

QBert_13

QBert_14

QBert_15

QBert_16

QBert_17

QBert_18

QBert_19

QBert_20

QBert_21

QBert_22

QBert_23

QBert_24

QBert_25

QBert_26

QBert_27

QBert_28

QBert_29
(helix-turn-helix DNA binding protein)

QBert_30

QBert_31

QBert_32

QBert_33
(ribbon-helix-helix DNA binding domain)

QBert_34

QBert_35

QBert_36

QBert_37

QBert_38

QBert_39

QBert_40

QBert_41

QBert_42

QBert_43

QBert_44

QBert_45

QBert_46

QBert_47

QBert_48

QBert_49

QBert_50
(helix-turn-helix DNA binding protein)

QBert_51

QBert_52

QBert_53

QBert_54

QBert_55

QBert_56

QBert_57
(helix-turn-helix DNA binding domain)

QBert_58
(helix-turn-helix DNA binding domain)

QBert_59

QBert_60

QBert_61

QBert_62

QBert_63

QBert_64

QBert_65

QBert_66

QBert_67

QBert_68

QBert_69

QBert_70

QBert_71

QBert_72

QBert_73

QBert_74

QBert_75

QBert_76

QBert_77

QBert_82
(methyltransferase)

QBert_83

QBert_84
(portal protein)

QBert_85

QBert_86

QBert_87
(ThyX-like thymidylate synthase)

QBert_88

QBert_89
(RNA ligase)

QBert_90
(LysM-domain)

QBert_91
(LysM domain)

QBert_92

QBert_93
(major capsid protein)

QBert_94
(Holliday junction resolvase)

QBert_95
(capsid decoration protein)

QBert_96

QBert_97

QBert_98

QBert_99

QBert_100

QBert_101

QBert_102
(membrane protein)

QBert_103

QBert_104

QBert_105

QBert_106

QBert_107

QBert_108

QBert_109

QBert_110

QBert_111

QBert_112

QBert_113

QBert_114

QBert_115
(N-acetyltransferase)

QBert_116

QBert_117

QBert_118

QBert_119

QBert_120

QBert_121
(tail sheath protein)

QBert_122

QBert_123

QBert_124
(tail assembly chaperone)

QBert_125
(tail assembly chaperone)

QBert_126
(tape measure protein)

QBert_127
(minor tail protein)

QBert_128

QBert_129

QBert_130
(minor tail protein)

QBert_131

QBert_132

QBert_133
(baseplate wedge protein)

QBert_134
(baseplate wedge protein)

QBert_135

QBert_136

QBert_137

QBert_138
(minor tail protein)

QBert_139
(minor tail protein)

QBert_140

QBert_141

QBert_142

QBert_143

QBert_144

QBert_145

QBert_146

QBert_149

QBert_150
(peptidyl tRNA hydrolase)

QBert_165
(minor tail protein, D-ala-D-ala carboxypeptidase)

QBert_166

QBert_178

QBert_179
(helicase)

QBert_180

QBert_181

QBert_182

QBert_183

QBert_184

QBert_185

QBert_186

QBert_187

QBert_188

QBert_189
(thymidylate kinase)

QBert_190
(aminotransferase)

QBert_191
(RNA binding domain)

QBert_192

QBert_193

QBert_194
(DnaC-like helicase loader)

QBert_195
(DnaB-like dsDNA helicase)

QBert_196

QBert_197
(DNA primase)

QBert_198

QBert_199
(chaperonin DnaJ-like)

QBert_200
(ssDNA binding domain)

QBert_201
(DNA polymerase III (alpha))

QBert_202
(Rf-1 peptide chain release factor)

QBert_203
(RecA-like DNA recombinase)

QBert_204
(Holliday junction resolvase)

QBert_205

QBert_206
(HNH endonuclease)

QBert_207
(RusA-like resolvase)

QBert_208

QBert_209

QBert_210

QBert_211

QBert_212
(glycoside hydrolase)

QBert_213

QBert_214

QBert_217

QBert_218

QBert_219

QBert_220

QBert_221

QBert_222

QBert_223

QBert_224

QBert_225
(Ro-like RNA binding protein)

QBert_226

QBert_227

QBert_228

QBert_229

QBert_230

QBert_231

QBert_232
(galactosaminyltransferase)

QBert_233

QBert_234
(glycosyltransferase)

QBert_235
(galactosyltransferase)

QBert_236
(nicotinate ribosyltransferase)

QBert_237
(glycosyltransferase)

QBert_238

QBert_239

QBert_240

QBert_241

QBert_242
(lysin A)

QBert_243
(membrane protein)

QBert_244
(lysin B)

QBert_245
(terminase)

QBert_246

QBert_247

QBert_248

QBert_249

QBert_250
(polynucleotide kinase)

QBert_251

QBert_252

QBert_253
(serine/threonine kinase)

QBert_254

QBert_255
(adenylosuccinate synthetase)

QBert_256

QBert_257

QBert_258

QBert_259

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK359345
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Not in Pitt Archives
SEA Lysate Titer 1.6E11
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload