Mycobacterium phage Tortoise16
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Detailed Information for Phage Tortoise16
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByThomas McNamara
Year Found2013
Location FoundHartford, CT USA
Finding InstitutionTrinity College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates41.749093 N, 72.691956 W Map
Discovery NotesThe sample was collected on the South side of the Trinity College Chapel, under a rose bush. It was taken from approximately 10 cm below the surface. The soil was dark brown, and slightly moist.
Naming NotesThis phage's name was inspired by the story of the Tortoise and the Hare.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 11, 2013
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage479
Genome length (bp)154584
Character of genome endsCircularly Permuted
GC Content64.8%
Sequencing NotesThis phage has a long run of Cs at its right end, similar to the genome of Bxb1. No sequencing technique has been able to reliably resolve the length of this stretch of nucleotides. From our best guess, it's about 30 Cs long, so we've included 30 Cs at the right end of the final sequence file.
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Cluster Life CycleLytic
Other Cluster Members

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Ading (C1)

Adlitam (C1)

Alice (C1)

Amataga (C1)

ArcherS7 (C1)

Astraea (C1)

Atlantean (C1)

Audrick (C1)

Ava3 (C1)

BadAgartude (C1)

Bangla1971 (C1)

Basquiat (C1)

BeanWater (C1)

Bigswole (C1)

Bipolarisk (C1)

Blackbrain (C1)

Bonray (C1)

Bread (C1)

Breeniome (C1)

Burrough (C1)

Bxz1 (C1)

Cactojaque (C1)

Cali (C1)

Cane17 (C1)

Catera (C1)

Chargie21 (C1)

CharlieB (C1)

ChickenPhender (C1)

Colt (C1)

Daffodil (C1)

Dandelion (C1)

Darko (C1)

Delilah (C1)

Derek (C1)

Dietrick (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Flabslab (C1)

Fludd (C1)

FoxtrotP1 (C1)

FrayBell (C1)

FudgeTart (C1)

Gabriel (C1)

Ghost (C1)

Gizmo (C1)

Grungle (C1)

Guwapp (C1)

HyRo (C1)

InigoMontoya (C1)

InterFolia (C1)

Iota (C1)

Jessibeth14 (C1)

JPickles (C1)

JustHall (C1)

Kamryn (C1)

Kboogie (C1)

Khaleesi (C1)

Koguma (C1)

LifeSavor (C1)

LinStu (C1)

Littleton (C1)

Loanshark (C1)

LolaVinca (C1)

LRRHood (C1)

Lukilu (C1)

Mangeria (C1)

McWolfish (C1)

Megamind (C1)

Melpomini (C1)

MikeLiesIn (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

Naija (C1)

Napoleon13 (C1)

Nappy (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

ParkTD (C1)

Patter (C1)

Phlegm (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pinkcreek (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

QBert (C1)

Rabinovish (C1)

Rahel (C1)

Rizal (C1)

RoMag (C1)

Roots515 (C1)

Salacia (C1)

Sauce (C1)

ScottMcG (C1)

Sebata (C1)

Shaqnato (C1)

Shelob (C1)

ShiaLabeouf (C1)

Shifa (C1)

Shnickers (C1)

Shrimp (C1)

SmallFry (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

StephanieG (C1)

Stubby (C1)

Teardrop (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Trinitium (C1)

Tyke (C1)

ValleyTerrace (C1)

Wally (C1)

Willis (C1)

YoungMoneyMata (C1)

Yucca (C1)

Zalkecks (C1)

Zeenon (C1)

ZygoTaiga (C1)

Myrna (C2)

Phabba (C2)

Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
Plaque NotesClear plaques

Mean diameter after 24 hrs: 0.275 mm
Mean diameter after 48 hrs: 0.625 mm
MorphotypeMyoviridae
Has been Phamerated?Yes
Gene List

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Tortoise16_1

Tortoise16_2
(nucleotidyltransferase)

Tortoise16_3

Tortoise16_4

Tortoise16_5

Tortoise16_6

Tortoise16_7

Tortoise16_8

Tortoise16_9

Tortoise16_10

Tortoise16_11

Tortoise16_12

Tortoise16_13

Tortoise16_14

Tortoise16_15

Tortoise16_16
(histidine triad nucleotide binding protein)

Tortoise16_17

Tortoise16_18

Tortoise16_19

Tortoise16_20

Tortoise16_21

Tortoise16_22

Tortoise16_23

Tortoise16_24

Tortoise16_25

Tortoise16_26

Tortoise16_27

Tortoise16_28

Tortoise16_29

Tortoise16_30
(helix-turn-helix DNA binding protein)

Tortoise16_31

Tortoise16_32

Tortoise16_33

Tortoise16_34
(ribbon-helix-helix DNA binding domain protein)

Tortoise16_35

Tortoise16_36

Tortoise16_37

Tortoise16_38

Tortoise16_39

Tortoise16_40

Tortoise16_41

Tortoise16_42

Tortoise16_43

Tortoise16_44

Tortoise16_45

Tortoise16_46

Tortoise16_47

Tortoise16_48

Tortoise16_49

Tortoise16_50

Tortoise16_51

Tortoise16_52

Tortoise16_53
(helix-turn-helix DNA binding protein)

Tortoise16_54

Tortoise16_55

Tortoise16_56

Tortoise16_57

Tortoise16_58

Tortoise16_59

Tortoise16_60
(helix-turn-helix DNA binding domain protein)

Tortoise16_61
(helix-turn-helix DNA binding domain protein)

Tortoise16_62

Tortoise16_63

Tortoise16_64

Tortoise16_65

Tortoise16_66

Tortoise16_67

Tortoise16_68

Tortoise16_69

Tortoise16_70

Tortoise16_71

Tortoise16_72

Tortoise16_73

Tortoise16_74

Tortoise16_75

Tortoise16_76

Tortoise16_77

Tortoise16_78

Tortoise16_79

Tortoise16_80

Tortoise16_81

Tortoise16_86
(methyltransferase)

Tortoise16_87

Tortoise16_88
(portal protein)

Tortoise16_89

Tortoise16_90

Tortoise16_91
(ThyX-like thymidylate synthase)

Tortoise16_92

Tortoise16_93
(RNA ligase)

Tortoise16_94
(LysM domain protein)

Tortoise16_95

Tortoise16_96

Tortoise16_97
(major capsid protein)

Tortoise16_98
(capsid decoration protein)

Tortoise16_99

Tortoise16_100

Tortoise16_101

Tortoise16_102

Tortoise16_103

Tortoise16_104

Tortoise16_105
(membrane protein)

Tortoise16_106

Tortoise16_107

Tortoise16_108

Tortoise16_109

Tortoise16_110

Tortoise16_111

Tortoise16_112

Tortoise16_113

Tortoise16_114

Tortoise16_115

Tortoise16_116

Tortoise16_117

Tortoise16_118
(N-acetyltransferase)

Tortoise16_119

Tortoise16_120

Tortoise16_121

Tortoise16_122

Tortoise16_123

Tortoise16_124
(tail sheath protein)

Tortoise16_125

Tortoise16_126

Tortoise16_127
(tail assembly chaperone)

Tortoise16_128
(tail assembly chaperone)

Tortoise16_129
(tape measure protein)

Tortoise16_130
(minor tail protein)

Tortoise16_131

Tortoise16_132

Tortoise16_133
(minor tail protein)

Tortoise16_134

Tortoise16_135

Tortoise16_136
(baseplate wedge protein)

Tortoise16_137
(baseplate wedge protein)

Tortoise16_138

Tortoise16_139

Tortoise16_140

Tortoise16_141
(minor tail protein)

Tortoise16_142
(minor tail protein)

Tortoise16_143

Tortoise16_144

Tortoise16_145

Tortoise16_146

Tortoise16_147

Tortoise16_148

Tortoise16_149

Tortoise16_150

Tortoise16_151

Tortoise16_155

Tortoise16_156
(peptidyl tRNA hydrolase)

Tortoise16_170
(HNH endonuclease)

Tortoise16_172
(D-ala-D-ala carboxypeptidase)

Tortoise16_173

Tortoise16_185

Tortoise16_186
(helicase)

Tortoise16_187

Tortoise16_188

Tortoise16_189

Tortoise16_190

Tortoise16_191

Tortoise16_192

Tortoise16_193

Tortoise16_194

Tortoise16_195
(thymidylate kinase)

Tortoise16_196
(aminotransferase)

Tortoise16_197
(RNA binding domain protein)

Tortoise16_198

Tortoise16_199

Tortoise16_200
(DnaC-like helicase loader)

Tortoise16_201
(DnaB-like dsDNA helicase)

Tortoise16_202

Tortoise16_203
(DNA primase)

Tortoise16_204

Tortoise16_205
(DnaJ-like chaperonin)

Tortoise16_206
(ssDNA binding domain protein)

Tortoise16_207

Tortoise16_208
(Rf-1 peptide chain release factor)

Tortoise16_209
(RecA-like DNA recombinase)

Tortoise16_210
(holliday junction resolvase)

Tortoise16_211

Tortoise16_212
(HNH endonuclease)

Tortoise16_213
(RusA-like resolvase)

Tortoise16_214

Tortoise16_215

Tortoise16_216

Tortoise16_217

Tortoise16_218
(glycoside hydrolase)

Tortoise16_219

Tortoise16_220

Tortoise16_223

Tortoise16_224

Tortoise16_225

Tortoise16_226

Tortoise16_227

Tortoise16_228

Tortoise16_229

Tortoise16_230

Tortoise16_231
(Ro-like RNA binding protein)

Tortoise16_232

Tortoise16_233

Tortoise16_234

Tortoise16_235

Tortoise16_236

Tortoise16_237

Tortoise16_238
(galactosaminyltransferase)

Tortoise16_239

Tortoise16_240
(glycosyltransferase)

Tortoise16_241
(galactosyltransferase)

Tortoise16_242
(nicotinate ribosyltransferase)

Tortoise16_243
(glycosyltransferase)

Tortoise16_244

Tortoise16_245

Tortoise16_246

Tortoise16_247

Tortoise16_248
(lysin A)

Tortoise16_249
(membrane protein)

Tortoise16_250
(lysin B)

Tortoise16_251
(terminase)

Tortoise16_252

Tortoise16_253

Tortoise16_254

Tortoise16_255

Tortoise16_256
(polynucleotide kinase)

Tortoise16_257

Tortoise16_258

Tortoise16_259
(serine/threonine kinase)

Tortoise16_260

Tortoise16_261

Tortoise16_262
(adenylosuccinate synthetase)

Tortoise16_263

Tortoise16_264

Tortoise16_265

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK359324
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
Pitt Freezer Box# 2
Pitt Freezer Box Grid# G4
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload