The Actinobacteriophage Database

Mycobacterium phage Tortoise16

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Detailed Information for Phage Tortoise16
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Thomas McNamara
Year Found	2013
Location Found	Hartford, CT USA
Finding Institution	Trinity College
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	Not entered
GPS Coordinates	41.749093 N, 72.691956 W Map
Discovery Notes	The sample was collected on the South side of the Trinity College Chapel, under a rose bush. It was taken from approximately 10 cm below the surface. The soil was dark brown, and slightly moist.
Naming Notes	This phage's name was inspired by the story of the Tortoise and the Hare.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Dec 11, 2013
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina Sequencing
Approximate Shotgun Coverage	479
Genome length (bp)	154584
Character of genome ends	Circularly Permuted
GC Content	64.8%
Sequencing Notes	This phage has a long run of Cs at its right end, similar to the genome of Bxb1. No sequencing technique has been able to reliably resolve the length of this stretch of nucleotides. From our best guess, it's about 30 Cs long, so we've included 30 Cs at the right end of the final sequence file.
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	C
Subcluster	C1
Cluster Life Cycle	Lytic
Other Cluster Members	Click to View Ading (C1) Adlitam (C1) Alice (C1) Amataga (C1) ArcherS7 (C1) Asterius (C1) Astraea (C1) Atlantean (C1) Audrick (C1) Ava3 (C1) Babyland (C1) BackyardAgain (C1) BadAgartude (C1) BananaFence (C1) Bangla1971 (C1) Basquiat (C1) BeanWater (C1) BigCity (C1) Bigswole (C1) Bipolarisk (C1) Blackbrain (C1) Blubba (C1) Bonray (C1) Bread (C1) Breeniome (C1) Burrough (C1) Bxz1 (C1) Cactojaque (C1) Cali (C1) Cane17 (C1) Capablanca (C1) Caravan (C1) Catera (C1) Chargie21 (C1) CharlieB (C1) ChaylaJr (C1) ChickenPhender (C1) Chomp (C1) CindyLou (C1) Colt (C1) Concombre (C1) Daffodil (C1) Dandelion (C1) Darko (C1) Delilah (C1) Delylah (C1) Derek (C1) Dietrick (C1) DirtMonster (C1) Drazdys (C1) DrPhinkDaddy (C1) DTDevon (C1) EasyJones (C1) EggyFarm (C1) EmmaElysia (C1) EmToTheThree (C1) Erdmann (C1) ErnieJ (C1) Essence (C1) ET08 (C1) Ewok (C1) Flabslab (C1) Fludd (C1) FoxtrotP1 (C1) FrayBell (C1) FudgeTart (C1) Gabriel (C1) Gamgam (C1) Ghost (C1) Gizmo (C1) Grasshills (C1) Grungle (C1) Guwapp (C1) Halldule (C1) HyRo (C1) I3 (C1) IkeLoa (C1) InigoMontoya (C1) InterFolia (C1) Iota (C1) Issimir (C1) Izajani (C1) Janiyra (C1) Jaygup (C1) JayJay (C1) Jedediah (C1) Jemmno (C1) Jessibeth14 (C1) JPickles (C1) JulietS (C1) JustHall (C1) Kamryn (C1) Kboogie (C1) Khaleesi (C1) Koguma (C1) Latch (C1) Lee7 (C1) LifeSavor (C1) LinStu (C1) Littleton (C1) Loanshark (C1) LolaVinca (C1) LordLeafolot (C1) LRRHood (C1) Lukilu (C1) Maby2021 (C1) Mangeria (C1) Matsumoto (C1) McGee (C1) McWolfish (C1) Megamind (C1) Melpomini (C1) MikeLiesIn (C1) Mikro (C1) Momo (C1) MoMoMixon (C1) Morizzled23 (C1) Naija (C1) Napoleon13 (C1) Nappy (C1) Naval22 (C1) NedWong (C1) Ngannou (C1) Nidhogg (C1) NoodleTree (C1) Norm (C1) NuevoMundo (C1) ParkTD (C1) Patter (C1) Phlegm (C1) Phox (C1) Phusco (C1) Pier (C1) Pinkcreek (C1) Pio (C1) Pivoine (C1) Pleione (C1) PurduePete (C1) QBert (C1) Quasimodo (C1) Rabinovish (C1) Rahel (C1) Rizal (C1) RoMag (C1) Ronan (C1) Roots515 (C1) Salacia (C1) Sauce (C1) ScottMcG (C1) Sebata (C1) Shaqnato (C1) Shelob (C1) ShiaLabeouf (C1) Shifa (C1) Shnickers (C1) Shrimp (C1) SilverDipper (C1) SmallFry (C1) Spec (C1) Specks (C1) Sprinklers (C1) Spud (C1) SrishMeg2525 (C1) StephanieG (C1) Stubby (C1) StubbySaigey (C1) Teardrop (C1) TinyTim (C1) Tonenili (C1) Tortoise16 (C1) Trinitium (C1) Turret (C1) Tyke (C1) ValleyTerrace (C1) Wally (C1) Willis (C1) Yassified (C1) YemiJoy2021 (C1) YoungMoneyMata (C1) Yucca (C1) Zalkecks (C1) Zeenon (C1) ZygoTaiga (C1) Greely (C2) Myrna (C2) Phabba (C2) ScoobyDoobyDoo (C2)
Annotating Institution	University of Pittsburgh
Annotation Status	In GenBank
Plaque Notes	Clear plaques Mean diameter after 24 hrs: 0.275 mm Mean diameter after 48 hrs: 0.625 mm
Morphotype	Myoviridae
Has been Phamerated?	Yes
Gene List	Click to View Tortoise16_1 Tortoise16_2 (nucleotidyltransferase) Tortoise16_3 Tortoise16_4 Tortoise16_5 Tortoise16_6 Tortoise16_7 Tortoise16_8 Tortoise16_9 Tortoise16_10 Tortoise16_11 Tortoise16_12 Tortoise16_13 Tortoise16_14 Tortoise16_15 Tortoise16_16 (histidine triad nucleotide binding protein) Tortoise16_17 Tortoise16_18 Tortoise16_19 Tortoise16_20 Tortoise16_21 Tortoise16_22 Tortoise16_23 Tortoise16_24 Tortoise16_25 Tortoise16_26 Tortoise16_27 Tortoise16_28 Tortoise16_29 Tortoise16_30 Tortoise16_31 Tortoise16_32 Tortoise16_33 Tortoise16_34 (ribbon-helix-helix DNA binding protein) Tortoise16_35 Tortoise16_36 Tortoise16_37 Tortoise16_38 Tortoise16_39 Tortoise16_40 Tortoise16_41 Tortoise16_42 Tortoise16_43 Tortoise16_44 Tortoise16_45 Tortoise16_46 Tortoise16_47 Tortoise16_48 Tortoise16_49 Tortoise16_50 Tortoise16_51 Tortoise16_52 Tortoise16_53 Tortoise16_54 Tortoise16_55 Tortoise16_56 Tortoise16_57 Tortoise16_58 Tortoise16_59 Tortoise16_60 (helix-turn-helix DNA binding domain protein) Tortoise16_61 (helix-turn-helix DNA binding protein) Tortoise16_62 Tortoise16_63 Tortoise16_64 Tortoise16_65 Tortoise16_66 Tortoise16_67 Tortoise16_68 Tortoise16_69 Tortoise16_70 Tortoise16_71 Tortoise16_72 Tortoise16_73 Tortoise16_74 Tortoise16_75 Tortoise16_76 Tortoise16_77 Tortoise16_78 Tortoise16_79 Tortoise16_80 Tortoise16_81 Tortoise16_86 Tortoise16_87 Tortoise16_88 (portal protein) Tortoise16_89 Tortoise16_90 Tortoise16_91 (ThyX-like thymidylate synthase) Tortoise16_92 Tortoise16_93 (RNA ligase) Tortoise16_94 Tortoise16_95 Tortoise16_96 Tortoise16_97 (major capsid protein) Tortoise16_98 Tortoise16_99 Tortoise16_100 Tortoise16_101 Tortoise16_102 Tortoise16_103 Tortoise16_104 Tortoise16_105 Tortoise16_106 Tortoise16_107 Tortoise16_108 Tortoise16_109 Tortoise16_110 Tortoise16_111 Tortoise16_112 Tortoise16_113 Tortoise16_114 Tortoise16_115 Tortoise16_116 Tortoise16_117 Tortoise16_118 (acetyltransferase) Tortoise16_119 Tortoise16_120 Tortoise16_121 Tortoise16_122 Tortoise16_123 Tortoise16_124 (tail sheath protein) Tortoise16_125 Tortoise16_126 Tortoise16_127 (tail assembly chaperone) Tortoise16_128 (tail assembly chaperone) Tortoise16_129 (tape measure protein) Tortoise16_130 (minor tail protein) Tortoise16_131 Tortoise16_132 Tortoise16_133 (minor tail protein) Tortoise16_134 Tortoise16_135 Tortoise16_136 (baseplate wedge protein) Tortoise16_137 (baseplate wedge protein) Tortoise16_138 Tortoise16_139 Tortoise16_140 Tortoise16_141 (minor tail protein) Tortoise16_142 (minor tail protein) Tortoise16_143 Tortoise16_144 Tortoise16_145 Tortoise16_146 Tortoise16_147 Tortoise16_148 Tortoise16_149 Tortoise16_150 Tortoise16_151 Tortoise16_155 Tortoise16_156 (peptidyl tRNA hydrolase) Tortoise16_170 (HNH endonuclease) Tortoise16_172 (minor tail protein) Tortoise16_173 Tortoise16_185 Tortoise16_186 (DNA helicase) Tortoise16_187 Tortoise16_188 Tortoise16_189 Tortoise16_190 Tortoise16_191 Tortoise16_192 Tortoise16_193 Tortoise16_194 Tortoise16_195 (thymidylate kinase) Tortoise16_196 (aminotransferase) Tortoise16_197 Tortoise16_198 Tortoise16_199 Tortoise16_200 (DnaC-like helicase loader) Tortoise16_201 (DnaB-like dsDNA helicase) Tortoise16_202 Tortoise16_203 (DNA primase) Tortoise16_204 Tortoise16_205 (DnaJ-like chaperonin) Tortoise16_206 (ssDNA binding protein) Tortoise16_207 Tortoise16_208 (RF-1 peptide chain release factor) Tortoise16_209 (RecA-like DNA recombinase) Tortoise16_210 (holliday junction resolvase) Tortoise16_211 Tortoise16_212 (HNH endonuclease) Tortoise16_213 (RusA-like resolvase) Tortoise16_214 Tortoise16_215 Tortoise16_216 Tortoise16_217 Tortoise16_218 Tortoise16_219 Tortoise16_220 Tortoise16_223 Tortoise16_224 Tortoise16_225 Tortoise16_226 Tortoise16_227 Tortoise16_228 Tortoise16_229 Tortoise16_230 Tortoise16_231 (Ro-like RNA binding protein) Tortoise16_232 Tortoise16_233 Tortoise16_234 Tortoise16_235 Tortoise16_236 Tortoise16_237 Tortoise16_238 (glycosyltransferase) Tortoise16_239 Tortoise16_240 (glycosyltransferase) Tortoise16_241 (glycosyltransferase) Tortoise16_242 (PnuC-like nicotinamide riboside transporter) Tortoise16_243 (glycosyltransferase) Tortoise16_244 Tortoise16_245 Tortoise16_246 Tortoise16_247 Tortoise16_248 (lysin A) Tortoise16_249 Tortoise16_250 (lysin B) Tortoise16_251 (terminase) Tortoise16_252 Tortoise16_253 Tortoise16_254 Tortoise16_255 Tortoise16_256 (polynucleotide kinase) Tortoise16_257 Tortoise16_258 Tortoise16_259 (serine/threonine kinase) Tortoise16_260 Tortoise16_261 Tortoise16_262 (PurA-like adenylosuccinate synthetase) Tortoise16_263 Tortoise16_264 Tortoise16_265
Submitted Minimal DNA Master File	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MK359324
Refseq Number	None yet
Published in Paper	Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	2
Pitt Freezer Box Grid#	G4
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
Final DNAMaster File	Download
GenBank File for Phamerator	Download

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