The Actinobacteriophage Database

Mycobacterium phage Wally

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Detailed Information for Phage Wally
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Rachel Nichole Walstead
Year Found	2010
Location Found	Mechanicsville, VA USA
Finding Institution	Virginia Commonwealth University
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	No
Isolation Temperature	Not entered
GPS Coordinates	37.616 N, 77.363475 W Map
Discovery Notes	Collected from soil under mulch (~3") in a flower bed Approximately 80°F weather in late August
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jan 29, 2011
Genome length (bp)	155299
Character of genome ends	Circularly Permuted
GC Content	64.7%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	C
Subcluster	C1
Cluster Life Cycle	Lytic
Other Cluster Members	Click to View Ading (C1) Adlitam (C1) Alice (C1) Amataga (C1) ArcherS7 (C1) Astraea (C1) Atlantean (C1) Audrick (C1) Ava3 (C1) BackyardAgain (C1) BadAgartude (C1) BananaFence (C1) Bangla1971 (C1) Basquiat (C1) BeanWater (C1) Bigswole (C1) Bipolarisk (C1) Blackbrain (C1) Bonray (C1) Bread (C1) Breeniome (C1) Burrough (C1) Bxz1 (C1) Cactojaque (C1) Cali (C1) Cane17 (C1) Catera (C1) Chargie21 (C1) CharlieB (C1) ChaylaJr (C1) ChickenPhender (C1) Colt (C1) Daffodil (C1) Dandelion (C1) Darko (C1) Delilah (C1) Derek (C1) Dietrick (C1) Drazdys (C1) DrPhinkDaddy (C1) DTDevon (C1) EmmaElysia (C1) EmToTheThree (C1) Erdmann (C1) ErnieJ (C1) Essence (C1) ET08 (C1) Ewok (C1) Flabslab (C1) Fludd (C1) FoxtrotP1 (C1) FrayBell (C1) FudgeTart (C1) Gabriel (C1) Ghost (C1) Gizmo (C1) Grungle (C1) Guwapp (C1) HyRo (C1) InigoMontoya (C1) InterFolia (C1) Iota (C1) Janiyra (C1) Jaygup (C1) Jessibeth14 (C1) JPickles (C1) JustHall (C1) Kamryn (C1) Kboogie (C1) Khaleesi (C1) Koguma (C1) Latch (C1) LifeSavor (C1) LinStu (C1) Littleton (C1) Loanshark (C1) LolaVinca (C1) LRRHood (C1) Lukilu (C1) Mangeria (C1) McWolfish (C1) Megamind (C1) Melpomini (C1) MikeLiesIn (C1) Momo (C1) MoMoMixon (C1) Morizzled23 (C1) Naija (C1) Napoleon13 (C1) Nappy (C1) Nidhogg (C1) NoodleTree (C1) Norm (C1) NuevoMundo (C1) ParkTD (C1) Patter (C1) Phlegm (C1) Phox (C1) Phusco (C1) Pier (C1) Pinkcreek (C1) Pio (C1) Pivoine (C1) Pleione (C1) QBert (C1) Quasimodo (C1) Rabinovish (C1) Rahel (C1) Rizal (C1) RoMag (C1) Ronan (C1) Roots515 (C1) Salacia (C1) Sauce (C1) ScottMcG (C1) Sebata (C1) Shaqnato (C1) Shelob (C1) ShiaLabeouf (C1) Shifa (C1) Shnickers (C1) Shrimp (C1) SmallFry (C1) Specks (C1) Sprinklers (C1) Spud (C1) StephanieG (C1) Stubby (C1) Teardrop (C1) TinyTim (C1) Tonenili (C1) Tortoise16 (C1) Trinitium (C1) Tyke (C1) ValleyTerrace (C1) Wally (C1) Willis (C1) YoungMoneyMata (C1) Yucca (C1) Zalkecks (C1) Zeenon (C1) ZygoTaiga (C1) Myrna (C2) Phabba (C2)
Annotating Institution	Unknown or unassigned
Annotation Status	In GenBank
Plaque Notes	Plaques range from 0.5mm - 2mm in diameter, all plates show large and small plaques. All plaques are clear
Morphotype	Myoviridae
Number of Genes	228
Number of tRNAs	34
Number of tmRNAs	1
Has been Phamerated?	Yes
Gene List	Click to View Wally_1 Wally_2 Wally_3 (NrdC-like protein) Wally_4 Wally_5 Wally_6 Wally_7 Wally_8 Wally_9 Wally_10 Wally_11 Wally_12 Wally_13 Wally_14 Wally_15 Wally_16 Wally_17 (histidine triad domain protein) Wally_18 Wally_19 Wally_20 Wally_21 Wally_22 Wally_23 Wally_24 Wally_25 Wally_26 Wally_27 Wally_28 (band 7-like protein) Wally_29 Wally_30 Wally_31 Wally_32 Wally_33 Wally_34 Wally_35 Wally_36 Wally_37 Wally_38 Wally_39 Wally_40 Wally_41 Wally_42 Wally_43 Wally_44 Wally_45 Wally_46 Wally_47 Wally_48 Wally_49 Wally_50 Wally_51 Wally_52 Wally_53 Wally_54 Wally_55 Wally_56 Wally_57 Wally_58 Wally_59 Wally_60 Wally_61 (HTH DNA binding domain protein) Wally_62 Wally_63 Wally_64 Wally_65 Wally_66 Wally_67 Wally_68 Wally_69 Wally_70 Wally_71 Wally_72 Wally_73 Wally_74 Wally_75 Wally_76 Wally_77 Wally_78 Wally_79 Wally_80 Wally_81 (ParB-like protein) Wally_87 Wally_88 Wally_89 (portal protein) Wally_90 Wally_91 Wally_92 (ThyX-like protein) Wally_93 Wally_94 Wally_95 (LysM domain) Wally_96 Wally_97 Wally_98 (major capsid protein) Wally_99 Wally_100 Wally_101 Wally_102 Wally_103 Wally_104 Wally_105 Wally_106 Wally_107 Wally_108 Wally_109 Wally_110 Wally_111 Wally_112 Wally_113 Wally_114 Wally_115 Wally_116 Wally_117 Wally_118 Wally_119 Wally_120 Wally_121 Wally_122 Wally_123 Wally_124 Wally_125 Wally_126 Wally_127 Wally_128 Wally_129 (tail assembly chaperone) Wally_130 (tail assembly chaperone) Wally_131 (tapemeasure protein) Wally_132 Wally_133 Wally_134 Wally_135 (minor tail protein) Wally_136 Wally_137 (minor tail protein) Wally_138 Wally_139 (baseplate J) Wally_140 Wally_141 Wally_142 Wally_143 Wally_144 Wally_145 Wally_146 Wally_147 Wally_148 Wally_149 Wally_150 Wally_151 Wally_155 Wally_156 (peptidyl-tRNA hydrolase) Wally_162 Wally_171 Wally_173 (D-Ala-D-Ala carboxypeptidase) Wally_174 Wally_186 Wally_187 Wally_188 (terminase) Wally_189 Wally_190 Wally_191 Wally_192 Wally_193 Wally_194 Wally_195 Wally_196 Wally_197 (ArgD-like protein) Wally_198 Wally_199 Wally_200 (DnaC-like protein) Wally_201 (DnaB-like helicase) Wally_202 Wally_203 (DNA primase) Wally_204 Wally_205 (DnaJ domain protein) Wally_206 Wally_207 (DNA polymerase III alpha subunit) Wally_208 (RF1 domain protein) Wally_209 (RecA-like protein) Wally_210 Wally_211 Wally_212 (homing-endonuclease) Wally_213 (RusA-like protein) Wally_214 Wally_215 Wally_216 Wally_217 Wally_218 Wally_219 Wally_220 Wally_223 Wally_224 Wally_225 Wally_226 Wally_227 Wally_228 Wally_229 Wally_230 Wally_231 (Ro protein) Wally_232 Wally_233 Wally_234 Wally_235 Wally_236 Wally_237 Wally_238 (glycosyltransferase) Wally_239 Wally_240 (galactosyltransferase) Wally_241 (galactosyltransferase) Wally_242 Wally_243 (glycosyltransferase) Wally_244 Wally_245 Wally_246 Wally_247 Wally_248 (lysin A) Wally_249 (holin) Wally_250 (lysin B) Wally_251 Wally_252 Wally_253 Wally_254 Wally_255 Wally_256 (Pnk) Wally_257 Wally_258 (serine/threonine protein kinase) Wally_259 Wally_260 Wally_261 (PurA) Wally_262 Wally_263
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	JN699625
Refseq Number	None yet
Published in Paper	Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status	Archived
SEA Designator	2010VACUwallyRNW
SEA Lysate Titer	6.0e10
Pitt Freezer Box#	3
Pitt Freezer Box Grid#	C11
Available Files
Plaque Picture	Download
EM Picture	Download
Final DNAMaster File	Download

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