Mycobacterium phage Khaleesi
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Detailed Information for Phage Khaleesi
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByAlex Kelsey & Ryan Schuck
Year Found2014
Location FoundColumbus, OH USA
Finding InstitutionThe Ohio State University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature25°C
GPS Coordinates39.994 N, 83.013 W Map
Naming NotesThe name for this phage came from Daenerys Targaryen from "Game of Thrones". Khaleesi happens to be a lytic phage, and it only seemed fitting that one of the most ruthless queens on the TV show -and book- could be represented by our phage.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 15, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage857
Genome length (bp)155251
Character of genome endsCircularly Permuted
GC Content64.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Cluster Life CycleLytic
Other Cluster Members

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Boonesok (None)

CLach (None)

DavidMina (None)

DitchDweller (None)

Elpis (None)

Genet (None)

Hard2get (None)

Hippocrates (None)

Kali (None)

Linnaeus (None)

LittleSpot (None)

Lsquared (None)

Marlex (None)

Minipoke (None)

MoBama (None)

PeeWee (None)

Pesky7 (None)

Phomnie (None)

Phyllis (None)

Roku (None)

Sagamore (None)

Samarai (None)

ShenziUR (None)

SuperCallie (None)

TinyC (None)

Trilla23 (None)

Turret (None)

Squidney (A3)

Ading (C1)

Adlitam (C1)

Alice (C1)

Amataga (C1)

ArcherS7 (C1)

Astraea (C1)

Atlantean (C1)

Atlas (C1)

Audrick (C1)

Ava3 (C1)

BadAgartude (C1)

Bangla1971 (C1)

Basquiat (C1)

BeanWater (C1)

Bigswole (C1)

Bipolarisk (C1)

Bonray (C1)

Bread (C1)

Breeniome (C1)

Burrough (C1)

Bxz1 (C1)

Cali (C1)

Cane17 (C1)

Catera (C1)

Chargie21 (C1)

CharlieB (C1)

ChickenPhender (C1)

Colt (C1)

Daffodil (C1)

Dandelion (C1)

Darko (C1)

Delilah (C1)

Derek (C1)

Dietrick (C1)

Donegal (C1)

Doodle1 (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Exxus285 (C1)

Flabslab (C1)

Fludd (C1)

FoxtrotP1 (C1)

FrayBell (C1)

FudgeTart (C1)

Gabriel (C1)

Ghost (C1)

Gizmo (C1)

Grungle (C1)

Guwapp (C1)

HarrySpotter (C1)

HyRo (C1)

InigoMontoya (C1)

InterFolia (C1)

Iota (C1)

Isee1101 (C1)

JennanaJr (C1)

Jessibeth14 (C1)

JustHall (C1)

Kamryn (C1)

Khaleesi (C1)

Koguma (C1)

LifeSavor (C1)

LinStu (C1)

Lionheart (C1)

Littleton (C1)

Loanshark (C1)

LolaVinca (C1)

LRRHood (C1)

Lukilu (C1)

McWolfish (C1)

Megamind (C1)

Melpomini (C1)

MePac (C1)

MikeLiesIn (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

MrsHay (C1)

Naija (C1)

Napoleon13 (C1)

Nappy (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

OaklaforG (C1)

ParkTD (C1)

Patch (C1)

Patter (C1)

Phlegm (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

QBert (C1)

Rabinovish (C1)

Rahel (C1)

Rizal (C1)

RoMag (C1)

Roots515 (C1)

Salacia (C1)

SandySandal (C1)

Sauce (C1)

ScottMcG (C1)

Sebastian (C1)

Sebata (C1)

ShankPowerGod (C1)

Shaqnato (C1)

Shelob (C1)

ShiaLabeouf (C1)

Shnickers (C1)

Shrimp (C1)

SmallFry (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

Stubby (C1)

Teardrop (C1)

TheO (C1)

Thuathua (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Tyke (C1)

ValleyTerrace (C1)

Wally (C1)

Willis (C1)

Yucca (C1)

Zalkecks (C1)

Zeenon (C1)

ZygoTaiga (C1)

Feauphix (C2)

Matcha (C2)

Myrna (C2)

Phabba (C2)

SimSimmers (C2)

SrishMeg2525 (C2)

Annotation StatusIn GenBank
Plaque NotesSmall Plaques (1.1-1.2 mm in diameter)
Clear Plaques
MorphotypeMyoviridae
Has been Phamerated?Yes
Gene List

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Khaleesi_1

Khaleesi_2

Khaleesi_3
(HNH endonuclease)

Khaleesi_4

Khaleesi_5

Khaleesi_6

Khaleesi_7

Khaleesi_8

Khaleesi_9

Khaleesi_10

Khaleesi_11

Khaleesi_12

Khaleesi_13

Khaleesi_14

Khaleesi_15

Khaleesi_16

Khaleesi_17

Khaleesi_18
(histidine triad domain protein)

Khaleesi_19

Khaleesi_20

Khaleesi_21

Khaleesi_22

Khaleesi_23

Khaleesi_24

Khaleesi_25

Khaleesi_26

Khaleesi_27

Khaleesi_28

Khaleesi_29

Khaleesi_30
(helix-turn-helix DNA binding protein)

Khaleesi_31

Khaleesi_32

Khaleesi_33

Khaleesi_34
(ribbon-helix-helix DNA binding domain protein)

Khaleesi_35

Khaleesi_36

Khaleesi_37

Khaleesi_38

Khaleesi_39

Khaleesi_40

Khaleesi_41

Khaleesi_42

Khaleesi_43

Khaleesi_44

Khaleesi_45

Khaleesi_46

Khaleesi_47

Khaleesi_48

Khaleesi_49

Khaleesi_50

Khaleesi_51

Khaleesi_52

Khaleesi_53

Khaleesi_54

Khaleesi_55
(helix-turn-helix DNA binding protein)

Khaleesi_56

Khaleesi_57

Khaleesi_58

Khaleesi_59

Khaleesi_60

Khaleesi_61

Khaleesi_62
(helix-turn-helix DNA binding domain protein)

Khaleesi_63
(helix-turn-helix DNA binding domain protein)

Khaleesi_64

Khaleesi_65

Khaleesi_66

Khaleesi_67

Khaleesi_68

Khaleesi_69

Khaleesi_70

Khaleesi_71

Khaleesi_72

Khaleesi_73

Khaleesi_74

Khaleesi_75

Khaleesi_76

Khaleesi_77

Khaleesi_78

Khaleesi_79

Khaleesi_80

Khaleesi_81

Khaleesi_82

Khaleesi_87
(methyltransferase)

Khaleesi_88

Khaleesi_89
(portal protein)

Khaleesi_90

Khaleesi_91

Khaleesi_92
(ThyX-like thymidylate synthase)

Khaleesi_93

Khaleesi_94
(RNA ligase)

Khaleesi_95
(LysM-domain protein)

Khaleesi_96

Khaleesi_97

Khaleesi_98
(major capsid protein)

Khaleesi_99
(holliday junction resolvase)

Khaleesi_100
(capsid decoration protein)

Khaleesi_101

Khaleesi_102

Khaleesi_103

Khaleesi_104

Khaleesi_105

Khaleesi_106

Khaleesi_107
(membrane protein)

Khaleesi_108

Khaleesi_109

Khaleesi_110

Khaleesi_111

Khaleesi_112

Khaleesi_113

Khaleesi_114

Khaleesi_115

Khaleesi_116

Khaleesi_117

Khaleesi_118

Khaleesi_119

Khaleesi_120
(N-acetyltransferase)

Khaleesi_121

Khaleesi_122

Khaleesi_123

Khaleesi_124

Khaleesi_125

Khaleesi_126
(tail sheath protein)

Khaleesi_127

Khaleesi_128

Khaleesi_129
(tail assembly chaperone)

Khaleesi_130
(tail assembly chaperone)

Khaleesi_131
(tape measure protein)

Khaleesi_132
(minor tail protein)

Khaleesi_133

Khaleesi_134

Khaleesi_135
(minor tail protein)

Khaleesi_136

Khaleesi_137

Khaleesi_138
(baseplate wedge protein)

Khaleesi_139
(baseplate wedge protein)

Khaleesi_140

Khaleesi_141

Khaleesi_142

Khaleesi_143
(minor tail protein)

Khaleesi_144
(minor tail protein)

Khaleesi_145

Khaleesi_146

Khaleesi_147

Khaleesi_148

Khaleesi_149

Khaleesi_150

Khaleesi_151

Khaleesi_155

Khaleesi_156
(peptidyl tRNA hydrolase)

Khaleesi_170
(HNH endonuclease)

Khaleesi_172
(minor tail protein)

Khaleesi_173

Khaleesi_185

Khaleesi_186

Khaleesi_187
(helicase)

Khaleesi_188

Khaleesi_189

Khaleesi_190

Khaleesi_191

Khaleesi_192

Khaleesi_193

Khaleesi_194

Khaleesi_195

Khaleesi_196

Khaleesi_197
(thymidylate kinase)

Khaleesi_198
(aminotransferase)

Khaleesi_199
(RNA binding domain protein)

Khaleesi_200

Khaleesi_201
(DnaC-like helicase loader)

Khaleesi_202
(DnaB-like dsDNA helicase)

Khaleesi_203

Khaleesi_204
(DNA primase)

Khaleesi_205

Khaleesi_206
(DnaJ-like chaperonin)

Khaleesi_207
(ssDNA binding domain protein)

Khaleesi_208
(DNA polymerase III (alpha))

Khaleesi_209
(RF-1 peptide chain release factor)

Khaleesi_210
(RecA-like DNA recombinase)

Khaleesi_211
(holliday junction resolvase)

Khaleesi_212

Khaleesi_213
(HNH endonuclease)

Khaleesi_214
(RusA-like resolvase)

Khaleesi_215

Khaleesi_216

Khaleesi_217

Khaleesi_218

Khaleesi_219
(glycoside hydrolase)

Khaleesi_220

Khaleesi_221

Khaleesi_224

Khaleesi_225

Khaleesi_226

Khaleesi_227

Khaleesi_228

Khaleesi_229

Khaleesi_230

Khaleesi_231

Khaleesi_232
(Ro-like RNA binding protein)

Khaleesi_233

Khaleesi_234

Khaleesi_235

Khaleesi_236

Khaleesi_237

Khaleesi_238

Khaleesi_239
(galactosaminyltransferase)

Khaleesi_240

Khaleesi_241
(glycosyltransferase)

Khaleesi_242
(galactosyltransferase)

Khaleesi_243
(nicotinate ribosyltransferase)

Khaleesi_244
(glycosyltransferase)

Khaleesi_245

Khaleesi_246

Khaleesi_247

Khaleesi_248

Khaleesi_249
(lysin A)

Khaleesi_250
(membrane protein)

Khaleesi_251
(lysin B)

Khaleesi_252
(terminase)

Khaleesi_253

Khaleesi_254

Khaleesi_255

Khaleesi_256

Khaleesi_257
(polynucleotide kinase)

Khaleesi_258

Khaleesi_259

Khaleesi_260
(serine/threonine kinase)

Khaleesi_261

Khaleesi_262

Khaleesi_263
(adenylosuccinate synthetase)

Khaleesi_264

Khaleesi_265

Khaleesi_266

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK112545
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 7.1x10^14 pfu/mL
Pitt Freezer Box# 12
Pitt Freezer Box Grid# B7
Available Files
EM PictureDownload
GenBank File for PhameratorDownload