Mycobacterium phage Bonray
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Detailed Information for Phage Bonray
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByJohn Brackett
Year Found2017
Location FoundDahlonega, GA USA
Finding InstitutionUniversity of North Georgia
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates34.533056 N, 83.985 W Map
Discovery NotesFound in well-established Urban garden bed with flowers and tree. There had been no recent rain and the temperature was 72 degrees Fahrenheit.The soil was fine and slightly damp.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 21, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage493
Genome length (bp)157364
Character of genome endsCircularly Permuted
GC Content64.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Cluster Life CycleLytic
Other Cluster Members

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Boonesok (None)

CLach (None)

DavidMina (None)

DitchDweller (None)

Elpis (None)

Genet (None)

Hard2get (None)

Hippocrates (None)

Kali (None)

Linnaeus (None)

LittleSpot (None)

Lsquared (None)

Marlex (None)

Minipoke (None)

MoBama (None)

PeeWee (None)

Pesky7 (None)

Phomnie (None)

Phyllis (None)

Roku (None)

Sagamore (None)

Samarai (None)

ShenziUR (None)

SuperCallie (None)

TinyC (None)

Trilla23 (None)

Turret (None)

RetimsGems (A1)

Squidney (A3)

Ading (C1)

Adlitam (C1)

Alice (C1)

Amataga (C1)

ArcherS7 (C1)

Astraea (C1)

Atlantean (C1)

Atlas (C1)

Audrick (C1)

Ava3 (C1)

BadAgartude (C1)

Bangla1971 (C1)

Basquiat (C1)

BeanWater (C1)

Bigswole (C1)

Bipolarisk (C1)

Bonray (C1)

Bread (C1)

Breeniome (C1)

Burrough (C1)

Bxz1 (C1)

Cali (C1)

Cane17 (C1)

Catera (C1)

Chargie21 (C1)

CharlieB (C1)

ChickenPhender (C1)

Colt (C1)

Daffodil (C1)

Dandelion (C1)

Darko (C1)

Delilah (C1)

Derek (C1)

Dietrick (C1)

Donegal (C1)

Doodle1 (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Exxus285 (C1)

Flabslab (C1)

Fludd (C1)

FoxtrotP1 (C1)

FrayBell (C1)

FudgeTart (C1)

Gabriel (C1)

Ghost (C1)

Gizmo (C1)

Grungle (C1)

Guwapp (C1)

HarrySpotter (C1)

HyRo (C1)

InigoMontoya (C1)

InterFolia (C1)

Iota (C1)

Isee1101 (C1)

JennanaJr (C1)

Jessibeth14 (C1)

JustHall (C1)

Kamryn (C1)

Khaleesi (C1)

Koguma (C1)

LifeSavor (C1)

LinStu (C1)

Lionheart (C1)

Littleton (C1)

Loanshark (C1)

LolaVinca (C1)

LRRHood (C1)

Lukilu (C1)

McWolfish (C1)

Megamind (C1)

Melpomini (C1)

MePac (C1)

MikeLiesIn (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

MrsHay (C1)

Naija (C1)

Napoleon13 (C1)

Nappy (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

OaklaforG (C1)

ParkTD (C1)

Patch (C1)

Patter (C1)

Phlegm (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

QBert (C1)

Rabinovish (C1)

Rahel (C1)

Rizal (C1)

RoMag (C1)

Roots515 (C1)

Salacia (C1)

SandySandal (C1)

Sauce (C1)

ScottMcG (C1)

Sebastian (C1)

Sebata (C1)

ShankPowerGod (C1)

Shaqnato (C1)

Shelob (C1)

ShiaLabeouf (C1)

Shnickers (C1)

Shrimp (C1)

SmallFry (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

Stubby (C1)

Teardrop (C1)

TheO (C1)

Thuathua (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Tyke (C1)

ValleyTerrace (C1)

Wally (C1)

Willis (C1)

Yucca (C1)

Zalkecks (C1)

Zeenon (C1)

ZygoTaiga (C1)

Feauphix (C2)

Matcha (C2)

Myrna (C2)

Phabba (C2)

SimSimmers (C2)

SrishMeg2525 (C2)

Annotation StatusIn GenBank
Plaque NotesBonray, produces plaques that are consistent in all aspects except size. The varying in sizes may be due to rapid mutation of the phage. The largest plaques are roughly 1.7mm, the medium ones about .63mm and the smallest plaques are .19mm. The large size of some plaques and the smooth edges indicates my phage may be a rapid lysis mutant. The plaques are clear and there is almost no turbidity.
MorphotypeMyoviridae
Has been Phamerated?Yes
Gene List

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Bonray_1

Bonray_2

Bonray_3
(HNH endonuclease)

Bonray_4

Bonray_5

Bonray_6

Bonray_7

Bonray_8

Bonray_9

Bonray_10

Bonray_11

Bonray_12

Bonray_13

Bonray_14

Bonray_15

Bonray_16

Bonray_17

Bonray_18
(histidine triad nucleotide binding protein)

Bonray_19

Bonray_20

Bonray_21

Bonray_22

Bonray_23

Bonray_24

Bonray_25

Bonray_26

Bonray_27

Bonray_28

Bonray_29

Bonray_30

Bonray_31
(helix-turn-helix DNA binding protein)

Bonray_32

Bonray_33

Bonray_34

Bonray_35
(ribbon-helix-helix DNA binding domain protein)

Bonray_36

Bonray_37

Bonray_38

Bonray_39

Bonray_40

Bonray_41

Bonray_42

Bonray_43

Bonray_44

Bonray_45

Bonray_46

Bonray_47

Bonray_48

Bonray_49

Bonray_50

Bonray_51

Bonray_52

Bonray_53

Bonray_54

Bonray_55

Bonray_56
(helix-turn-helix DNA binding protein)

Bonray_57

Bonray_58

Bonray_59

Bonray_60

Bonray_61

Bonray_62

Bonray_63
(helix-turn-helix DNA binding domain protein)

Bonray_64
(helix-turn-helix DNA binding domain protein)

Bonray_65

Bonray_66

Bonray_67

Bonray_68

Bonray_69

Bonray_70

Bonray_71

Bonray_72

Bonray_73

Bonray_74

Bonray_75

Bonray_76

Bonray_77

Bonray_78

Bonray_79

Bonray_80

Bonray_81

Bonray_82

Bonray_83

Bonray_88
(methyltransferase)

Bonray_89

Bonray_90
(portal protein)

Bonray_91

Bonray_92

Bonray_93
(ThyX-like thymidylate synthase)

Bonray_94

Bonray_95
(RNA ligase)

Bonray_96
(LysM-domain protein)

Bonray_97

Bonray_98

Bonray_99
(major capsid protein)

Bonray_100
(holliday junction resolvase)

Bonray_101
(capsid decoration protein)

Bonray_102

Bonray_103

Bonray_104

Bonray_105

Bonray_106

Bonray_107

Bonray_108
(membrane protein)

Bonray_109

Bonray_110

Bonray_111

Bonray_112

Bonray_113

Bonray_114

Bonray_115

Bonray_116

Bonray_117

Bonray_118

Bonray_119

Bonray_120

Bonray_121
(N-acetyltransferase)

Bonray_122

Bonray_123

Bonray_124

Bonray_125

Bonray_126

Bonray_127

Bonray_128
(tail sheath protein)

Bonray_129

Bonray_130

Bonray_131
(tail assembly chaperone)

Bonray_132
(tail assembly chaperone)

Bonray_133
(tape measure protein)

Bonray_134
(minor tail protein)

Bonray_135

Bonray_136

Bonray_137
(minor tail protein)

Bonray_138

Bonray_139

Bonray_140
(baseplate wedge protein)

Bonray_141
(baseplate wedge protein)

Bonray_142

Bonray_143

Bonray_144

Bonray_145
(minor tail protein)

Bonray_146
(minor tail protein)

Bonray_147

Bonray_148

Bonray_149

Bonray_150

Bonray_151

Bonray_152

Bonray_153

Bonray_157

Bonray_158
(peptidyl tRNA hydrolase)

Bonray_168
(HNH endonuclease)

Bonray_173
(HNH endonuclease)

Bonray_175
(minor tail protein)

Bonray_176

Bonray_188

Bonray_189

Bonray_190
(helicase)

Bonray_191

Bonray_192

Bonray_193

Bonray_194

Bonray_195

Bonray_196

Bonray_197

Bonray_198

Bonray_199

Bonray_200
(thymidylate kinase)

Bonray_201
(aminotransferase)

Bonray_202
(RNA binding domain protein)

Bonray_203

Bonray_204
(DnaC-like helicase loader)

Bonray_205
(DnaB-like dsDNA helicase)

Bonray_206

Bonray_207
(DNA primase)

Bonray_208

Bonray_209
(DnaJ-like chaperonin)

Bonray_210
(ssDNA binding domain protein)

Bonray_211
(DNA polymerase III (alpha))

Bonray_212
(RF-1 peptide chain release factor)

Bonray_213
(RecA-like DNA recombinase)

Bonray_214
(holliday junction resolvase)

Bonray_215

Bonray_216
(HNH endonuclease)

Bonray_217
(RusA-like resolvase)

Bonray_218

Bonray_219

Bonray_220

Bonray_221

Bonray_222
(glycoside hydrolase)

Bonray_223

Bonray_224

Bonray_227

Bonray_228

Bonray_229

Bonray_230

Bonray_231

Bonray_232

Bonray_233

Bonray_234

Bonray_235
(Ro-like RNA binding protein)

Bonray_236

Bonray_237

Bonray_238

Bonray_239

Bonray_240

Bonray_241

Bonray_242
(galactosaminyltransferase)

Bonray_243

Bonray_244
(glycosyltransferase)

Bonray_245
(galactosyltransferase)

Bonray_246
(nicotinate ribosyltransferase)

Bonray_247
(glycosyltransferase)

Bonray_248

Bonray_249

Bonray_250

Bonray_251

Bonray_252
(HNH endonuclease)

Bonray_253
(lysin A)

Bonray_254
(membrane protein)

Bonray_255
(lysin B)

Bonray_256
(terminase)

Bonray_257

Bonray_258

Bonray_259

Bonray_260

Bonray_261
(polynucleotide kinase)

Bonray_262

Bonray_263

Bonray_264
(serine/threonine kinase)

Bonray_265

Bonray_266

Bonray_267
(adenylosuccinate synthetase)

Bonray_268

Bonray_269

Bonray_270

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK112533
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
SEA Lysate Titer 5x10e9
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload