Mycobacterium phage I3
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Detailed Information for Phage I3
Discovery Information
Isolation HostMycobacterium smegmatis SN2
Found ByC.V. Sundar Raj
Year Found1970
Location FoundBangalore, India
ProgramUnavailable
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates13.021525 N, 77.567078 E Map
Discovery Notesforms turbid plaques on M. smegmatis SN2 and clear plaques on M. smegmatis EB165.
This phage has a wide host range on related mycobacteria.
I3 mediates transduction in M. smegmatis. Tranduction has been demonstrated for histidine, glycine, arginine, alanine and adenine markers.
Raj, C. V. & Ramakrishnan, T. (1970). Transduction in Mycobacterium
smegmatis. Nature 228, 280–281.

Chen J, Kriakov J, Singh A, Jacobs WR, Besra GS, Bhatt A. Defects in glycopeptidolipid biosynthesis confer phage I3 resistance in Mycobacterium smegmatis. Microbiology (Reading). 2009 Dec;155(Pt 12):4050-4057. doi: 10.1099/mic.0.033209-0. Epub 2009 Sep 10. PMID: 19744987.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMar 9, 2021
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage308
Genome length (bp)156127
Character of genome endsCircularly Permuted
GC Content64.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Cluster Life CycleLytic
Other Cluster Members

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Ading (C1)

Adlitam (C1)

Alice (C1)

Amataga (C1)

ArcherS7 (C1)

Astraea (C1)

Atlantean (C1)

Audrick (C1)

Ava3 (C1)

BackyardAgain (C1)

BadAgartude (C1)

BananaFence (C1)

Bangla1971 (C1)

Basquiat (C1)

BeanWater (C1)

Bigswole (C1)

Bipolarisk (C1)

Blackbrain (C1)

Bonray (C1)

Bread (C1)

Breeniome (C1)

Burrough (C1)

Bxz1 (C1)

Cactojaque (C1)

Cali (C1)

Cane17 (C1)

Catera (C1)

Chargie21 (C1)

CharlieB (C1)

ChaylaJr (C1)

ChickenPhender (C1)

Colt (C1)

Daffodil (C1)

Dandelion (C1)

Darko (C1)

Delilah (C1)

Delylah (C1)

Derek (C1)

Dietrick (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EasyJones (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Flabslab (C1)

Fludd (C1)

FoxtrotP1 (C1)

FrayBell (C1)

FudgeTart (C1)

Gabriel (C1)

Ghost (C1)

Gizmo (C1)

Grungle (C1)

Guwapp (C1)

HyRo (C1)

I3 (C1)

InigoMontoya (C1)

InterFolia (C1)

Iota (C1)

Izajani (C1)

Janiyra (C1)

Jaygup (C1)

Jemmno (C1)

Jessibeth14 (C1)

JPickles (C1)

JustHall (C1)

Kamryn (C1)

Kboogie (C1)

Khaleesi (C1)

Koguma (C1)

Latch (C1)

LifeSavor (C1)

LinStu (C1)

Littleton (C1)

Loanshark (C1)

LolaVinca (C1)

LRRHood (C1)

Lukilu (C1)

Mangeria (C1)

McWolfish (C1)

Megamind (C1)

Melpomini (C1)

MikeLiesIn (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

Naija (C1)

Napoleon13 (C1)

Nappy (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

ParkTD (C1)

Patter (C1)

Phlegm (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pinkcreek (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

QBert (C1)

Quasimodo (C1)

Rabinovish (C1)

Rahel (C1)

Rizal (C1)

RoMag (C1)

Ronan (C1)

Roots515 (C1)

Salacia (C1)

Sauce (C1)

ScottMcG (C1)

Sebata (C1)

Shaqnato (C1)

Shelob (C1)

ShiaLabeouf (C1)

Shifa (C1)

Shnickers (C1)

Shrimp (C1)

SilverDipper (C1)

SmallFry (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

StephanieG (C1)

Stubby (C1)

Teardrop (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Trinitium (C1)

Turret (C1)

Tyke (C1)

ValleyTerrace (C1)

Wally (C1)

Willis (C1)

YoungMoneyMata (C1)

Yucca (C1)

Zalkecks (C1)

Zeenon (C1)

ZygoTaiga (C1)

Myrna (C2)

Phabba (C2)

ScoobyDoobyDoo (C2)

Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
MorphotypeMyoviridae
Number of Genes242
Number of tRNAs34
Number of tmRNAs1
Has been Phamerated?Yes
Gene List

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I3_1

I3_2

I3_3
(nucleotidyl transferase)

I3_4

I3_5

I3_6

I3_7

I3_8

I3_9

I3_10

I3_11

I3_12

I3_13

I3_14

I3_15

I3_16

I3_17

I3_18

I3_19

I3_20
(histidine triad nucleotide binding protein)

I3_21

I3_22

I3_23

I3_24

I3_25

I3_26

I3_27

I3_28

I3_29

I3_30

I3_31

I3_32

I3_33
(DNA binding protein)

I3_34

I3_35

I3_36

I3_37
(DNA binding protein)

I3_38

I3_39

I3_40

I3_41

I3_42

I3_43

I3_44

I3_45

I3_46

I3_47

I3_48

I3_49

I3_50

I3_51

I3_52

I3_53

I3_54

I3_55

I3_56

I3_57

I3_58
(DNA binding protein)

I3_59

I3_60

I3_61

I3_62

I3_63

I3_64

I3_65
(helix-turn-helix DNA binding protein)

I3_66
(DNA binding protein)

I3_67

I3_68

I3_69

I3_70

I3_71

I3_72

I3_73

I3_74

I3_75

I3_76

I3_77

I3_78

I3_79

I3_80

I3_81

I3_82

I3_83

I3_84

I3_85

I3_86

I3_87

I3_88
(ParB-like nuclease domain)

I3_94

I3_95

I3_96
(portal protein)

I3_97

I3_98

I3_99
(ThyX-like thymidylate synthase)

I3_100

I3_101
(phosphodiesterase)

I3_102

I3_103

I3_104

I3_105
(major capsid protein)

I3_106
(DNA binding protein)

I3_107

I3_108
(head decoration protein)

I3_109

I3_110

I3_111

I3_112

I3_113

I3_114

I3_115

I3_116

I3_117

I3_118

I3_119

I3_120

I3_121

I3_122

I3_123

I3_124

I3_125

I3_126
(acetyltransferase)

I3_127

I3_128

I3_129
(ParB-like nuclease domain)

I3_130
(minor capsid protein)

I3_131

I3_132
(tail sheath protein)

I3_133

I3_134

I3_135
(tail assembly chaperone)

I3_136
(tail assembly chaperone)

I3_137
(tape measure protein)

I3_138
(minor tail protein)

I3_139

I3_140

I3_141
(minor tail protein)

I3_142
(minor tail protein)

I3_143

I3_144
(baseplate wedge protein)

I3_145
(baseplate J protein)

I3_146

I3_147

I3_148

I3_149
(minor tail protein)

I3_150
(minor tail protein)

I3_151

I3_152

I3_153

I3_154

I3_155

I3_156

I3_157

I3_158

I3_159

I3_160

I3_161

I3_165

I3_166
(peptidyl tRNA hydrolase)

I3_181
(minor tail protein)

I3_182

I3_194

I3_195
(DNA binding protein)

I3_196
(DNA helicase)

I3_197

I3_198

I3_199

I3_200

I3_201
(DNA binding protein)

I3_202

I3_203

I3_204

I3_205
(helix-turn-helix DNA binding domain, MerR-like)

I3_206

I3_207

I3_208
(aminotransferase)

I3_209
(RNA binding protein)

I3_210

I3_211

I3_212
(DnaC-like helicase loader)

I3_213
(DnaB-like dsDNA helicase)

I3_214

I3_215
(DNA primase)

I3_216

I3_217
(DnaJ-like chaperonin)

I3_218
(ssDNA binding protein)

I3_219
(DnaE-like DNA polymerase III)

I3_220

I3_221
(RecA-like DNA recombinase)

I3_222
(Holliday junction resolvase)

I3_223

I3_224
(Cas4 family exonuclease)

I3_225

I3_226

I3_227
(DNA binding protein)

I3_228

I3_229
(DNA binding protein)

I3_230
(hydrolase)

I3_231

I3_232

I3_235

I3_236

I3_237

I3_238

I3_239

I3_240

I3_241

I3_242

I3_243
(Ro-like RNA binding protein)

I3_244

I3_245

I3_246

I3_247

I3_248

I3_249

I3_250
(glycosyltransferase)

I3_251

I3_252
(glycosyltransferase)

I3_253
(glycosyltransferase)

I3_254
(PnuC-like nicotinamide riboside transporter)

I3_255
(glycosyltransferase)

I3_256

I3_257

I3_258

I3_259

I3_260
(lysin A)

I3_261
(holin)

I3_262
(lysin B)

I3_263
(terminase)

I3_264

I3_265

I3_266

I3_267

I3_268
(polynucleotide kinase)

I3_269

I3_270
(serine/threonine kinase)

I3_271
(Ku-like dsDNA break-binding protein)

I3_272

I3_273
(PurA-like adenylosuccinate synthetase)

I3_274

I3_275

I3_276

I3_277

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMW822148
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload