Mycobacterium phage Derek
Know something about this phage that we don't? Modify its data.
Detailed Information for Phage Derek
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByBarbara Tsaousis
Year Found2015
Location FoundBethlehem, PA USA
Finding InstitutionLehigh University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature-1°C
GPS Coordinates40.6065 N, 75.372273 W Map
Discovery NotesDerek was discovered on Lehigh University's campus. The soil sample was taken from approximately 2 inches beneath the surface.
Naming NotesNamed after the fictional character Derek Shepherd, who was a neurosurgeon on ABC's Grey's Anatomy.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 5, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage423
Genome length (bp)156199
Character of genome endsCircularly Permuted
GC Content64.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Cluster Life CycleLytic
Other Cluster Members

Click to View

Boonesok (None)

CLach (None)

DavidMina (None)

DitchDweller (None)

Elpis (None)

Genet (None)

Hard2get (None)

Hippocrates (None)

Kali (None)

Linnaeus (None)

LittleSpot (None)

Lsquared (None)

Marlex (None)

Minipoke (None)

MoBama (None)

PeeWee (None)

Pesky7 (None)

Phomnie (None)

Phyllis (None)

Roku (None)

Sagamore (None)

Samarai (None)

ShenziUR (None)

SuperCallie (None)

TinyC (None)

Trilla23 (None)

Turret (None)

RetimsGems (A1)

Squidney (A3)

Ading (C1)

Adlitam (C1)

Alice (C1)

Amataga (C1)

ArcherS7 (C1)

Astraea (C1)

Atlantean (C1)

Atlas (C1)

Audrick (C1)

Ava3 (C1)

BadAgartude (C1)

Bangla1971 (C1)

Basquiat (C1)

BeanWater (C1)

Bigswole (C1)

Bipolarisk (C1)

Bonray (C1)

Bread (C1)

Breeniome (C1)

Burrough (C1)

Bxz1 (C1)

Cali (C1)

Cane17 (C1)

Catera (C1)

Chargie21 (C1)

CharlieB (C1)

ChickenPhender (C1)

Colt (C1)

Daffodil (C1)

Dandelion (C1)

Darko (C1)

Delilah (C1)

Derek (C1)

Dietrick (C1)

Donegal (C1)

Doodle1 (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Exxus285 (C1)

Flabslab (C1)

Fludd (C1)

FoxtrotP1 (C1)

FrayBell (C1)

FudgeTart (C1)

Gabriel (C1)

Ghost (C1)

Gizmo (C1)

Grungle (C1)

Guwapp (C1)

HarrySpotter (C1)

HyRo (C1)

InigoMontoya (C1)

InterFolia (C1)

Iota (C1)

Isee1101 (C1)

JennanaJr (C1)

Jessibeth14 (C1)

JustHall (C1)

Kamryn (C1)

Khaleesi (C1)

Koguma (C1)

LifeSavor (C1)

LinStu (C1)

Lionheart (C1)

Littleton (C1)

Loanshark (C1)

LolaVinca (C1)

LRRHood (C1)

Lukilu (C1)

McWolfish (C1)

Megamind (C1)

Melpomini (C1)

MePac (C1)

MikeLiesIn (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

MrsHay (C1)

Naija (C1)

Napoleon13 (C1)

Nappy (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

OaklaforG (C1)

ParkTD (C1)

Patch (C1)

Patter (C1)

Phlegm (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

QBert (C1)

Rabinovish (C1)

Rahel (C1)

Rizal (C1)

RoMag (C1)

Roots515 (C1)

Salacia (C1)

SandySandal (C1)

Sauce (C1)

ScottMcG (C1)

Sebastian (C1)

Sebata (C1)

ShankPowerGod (C1)

Shaqnato (C1)

Shelob (C1)

ShiaLabeouf (C1)

Shnickers (C1)

Shrimp (C1)

SmallFry (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

Stubby (C1)

Teardrop (C1)

TheO (C1)

Thuathua (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Tyke (C1)

ValleyTerrace (C1)

Wally (C1)

Willis (C1)

Yucca (C1)

Zalkecks (C1)

Zeenon (C1)

ZygoTaiga (C1)

Feauphix (C2)

Matcha (C2)

Myrna (C2)

Phabba (C2)

SimSimmers (C2)

SrishMeg2525 (C2)

Annotation StatusIn GenBank
Plaque NotesPlaques were originally very small (approx. 1mm) and turbid when phages were left to infect M. smegmatis at 37 degrees Celsius, but it was discovered that they developed an opaque, shadowy region surrounding each plaque upon spending excessive time at cold temperatures (4 degrees Celsius). This shadowy region was distinct from the plaques and was confirmed by a repeated trial. It was also confirmed that said regions were not present after undergoing regular incubation at 37 degrees. Picture provided is of a plate that was not subjected to colder temperatures.
MorphotypeMyoviridae
Has been Phamerated?Yes
Gene List

Click to View

Derek_1

Derek_2

Derek_3
(nucleotidyltransferase)

Derek_4

Derek_5

Derek_6

Derek_7

Derek_8

Derek_9

Derek_10

Derek_11

Derek_12

Derek_13

Derek_14

Derek_15

Derek_16

Derek_17

Derek_18
(histidine triad nucleotide binding protein)

Derek_19

Derek_20

Derek_21

Derek_22

Derek_23

Derek_24

Derek_25

Derek_26
(HNH endonuclease)

Derek_27

Derek_28

Derek_29

Derek_30
(band-7-like membrane protein)

Derek_31

Derek_32

Derek_33

Derek_34

Derek_35

Derek_36

Derek_37

Derek_38

Derek_39

Derek_40

Derek_41

Derek_42

Derek_43

Derek_44

Derek_45

Derek_46

Derek_47

Derek_48

Derek_49

Derek_50

Derek_51

Derek_52

Derek_53

Derek_54

Derek_55

Derek_56

Derek_57

Derek_58

Derek_59

Derek_60

Derek_61

Derek_62

Derek_63
(helix-turn-helix DNA binding protein)

Derek_64

Derek_65

Derek_66

Derek_67

Derek_68

Derek_69

Derek_70

Derek_71

Derek_72

Derek_73

Derek_74

Derek_75

Derek_76

Derek_77

Derek_78

Derek_79

Derek_80

Derek_81

Derek_82

Derek_83
(ParB-like nuclease domain protein)

Derek_88

Derek_89

Derek_90
(portal protein)

Derek_91

Derek_92

Derek_93
(thymidylate synthase)

Derek_94

Derek_95
(RNA ligase)

Derek_96
(HNH endonuclease)

Derek_97
(LysM-domain protein)

Derek_98

Derek_99

Derek_100
(major capsid protein)

Derek_101

Derek_102

Derek_103

Derek_104

Derek_105

Derek_106

Derek_107

Derek_108

Derek_109

Derek_110

Derek_111

Derek_112

Derek_113

Derek_114

Derek_115

Derek_116

Derek_117

Derek_118

Derek_119

Derek_120

Derek_121

Derek_122

Derek_123

Derek_124

Derek_125

Derek_126
(tail sheath protein)

Derek_127

Derek_128

Derek_129
(tail assembly chaperone)

Derek_130
(tail assembly chaperone)

Derek_131
(tape measure protein)

Derek_132

Derek_133

Derek_134

Derek_135

Derek_136

Derek_137
(minor tail protein)

Derek_138

Derek_139
(baseplate J protein)

Derek_140

Derek_141

Derek_142

Derek_143

Derek_144

Derek_145

Derek_146

Derek_147

Derek_148

Derek_149

Derek_150

Derek_151

Derek_155

Derek_156
(peptidyl tRNA hydrolase)

Derek_162

Derek_172
(D-alanyl-D-alanine carboxypeptidase)

Derek_173

Derek_184

Derek_185
(helicase)

Derek_186

Derek_187

Derek_188

Derek_189

Derek_190

Derek_191

Derek_192

Derek_193

Derek_194

Derek_195

Derek_196

Derek_197

Derek_198

Derek_199
(DnaC-like helicase loader)

Derek_200
(DnaB-like dsDNA helicase)

Derek_201

Derek_202
(DNA primase)

Derek_203

Derek_204
(DnaJ-like chaperonin)

Derek_205

Derek_206
(DNA polymerase III alpha subunit)

Derek_207
(release factor 1 protein)

Derek_208
(RecA-like DNA recombinase)

Derek_209
(resolvase)

Derek_210

Derek_211
(HNH endonuclease)

Derek_212
(RusA-like resolvase)

Derek_213

Derek_214

Derek_215

Derek_216

Derek_217
(hydrolase)

Derek_218

Derek_219
(HNH endonuclease)

Derek_220

Derek_223

Derek_224

Derek_225

Derek_226

Derek_227

Derek_228

Derek_229
(Ro domain RNA binding protein)

Derek_230

Derek_231

Derek_232

Derek_233

Derek_234

Derek_235

Derek_236
(glycosyltransferase)

Derek_237

Derek_238
(glycosyltransferase)

Derek_239
(galactosyltransferase)

Derek_240

Derek_241
(glycosyltransferase)

Derek_242

Derek_243

Derek_244

Derek_245

Derek_246
(lysin A)

Derek_247

Derek_248
(lysin B)

Derek_249
(terminase)

Derek_250

Derek_251

Derek_252

Derek_253

Derek_254

Derek_255

Derek_256

Derek_257
(serine/threonine kinase)

Derek_258

Derek_259

Derek_260
(PurA-like adenylosuccinate synthase)

Derek_261

Derek_262

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH316561
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 3.60*10^11 pfu/mL
Date of SEA Lysate Titering Mar 30, 2015
Pitt Freezer Box# 15
Pitt Freezer Box Grid# G5
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload