Mycobacterium phage SmallFry
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Detailed Information for Phage SmallFry
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByNicolette Driscoll
Year Found2012
Location FoundProvidence, RI USA
Finding InstitutionBrown University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates41.83 N, 71.3982 W Map
Discovery NotesSample was collected from moist, gritty soil about 2 inches deep. Temperature was approx. 73 degrees Fahrenheit.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 24, 2013
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIon Torrent Sequencing
Approximate Shotgun Coverage380
Sanger Finishing Reactions18
Genome length (bp)155433
Character of genome endsCircularly Permuted
GC Content64.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Cluster Life CycleLytic
Other Cluster Members

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Ading (C1)

Adlitam (C1)

Alice (C1)

Amataga (C1)

ArcherS7 (C1)

Astraea (C1)

Atlantean (C1)

Audrick (C1)

Ava3 (C1)

BackyardAgain (C1)

BadAgartude (C1)

Bangla1971 (C1)

Basquiat (C1)

BeanWater (C1)

Bigswole (C1)

Bipolarisk (C1)

Blackbrain (C1)

Bonray (C1)

Bread (C1)

Breeniome (C1)

Burrough (C1)

Bxz1 (C1)

Cactojaque (C1)

Cali (C1)

Cane17 (C1)

Catera (C1)

Chargie21 (C1)

CharlieB (C1)

ChaylaJr (C1)

ChickenPhender (C1)

Colt (C1)

Daffodil (C1)

Dandelion (C1)

Darko (C1)

Delilah (C1)

Derek (C1)

Dietrick (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Flabslab (C1)

Fludd (C1)

FoxtrotP1 (C1)

FrayBell (C1)

FudgeTart (C1)

Gabriel (C1)

Ghost (C1)

Gizmo (C1)

Grungle (C1)

Guwapp (C1)

HyRo (C1)

InigoMontoya (C1)

InterFolia (C1)

Iota (C1)

Janiyra (C1)

Jessibeth14 (C1)

JPickles (C1)

JustHall (C1)

Kamryn (C1)

Kboogie (C1)

Khaleesi (C1)

Koguma (C1)

LifeSavor (C1)

LinStu (C1)

Littleton (C1)

Loanshark (C1)

LolaVinca (C1)

LRRHood (C1)

Lukilu (C1)

Mangeria (C1)

McWolfish (C1)

Megamind (C1)

Melpomini (C1)

MikeLiesIn (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

Naija (C1)

Napoleon13 (C1)

Nappy (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

ParkTD (C1)

Patter (C1)

Phlegm (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pinkcreek (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

QBert (C1)

Quasimodo (C1)

Rabinovish (C1)

Rahel (C1)

Rizal (C1)

RoMag (C1)

Roots515 (C1)

Salacia (C1)

Sauce (C1)

ScottMcG (C1)

Sebata (C1)

Shaqnato (C1)

Shelob (C1)

ShiaLabeouf (C1)

Shifa (C1)

Shnickers (C1)

Shrimp (C1)

SmallFry (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

StephanieG (C1)

Stubby (C1)

Teardrop (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Trinitium (C1)

Tyke (C1)

ValleyTerrace (C1)

Wally (C1)

Willis (C1)

YoungMoneyMata (C1)

Yucca (C1)

Zalkecks (C1)

Zeenon (C1)

ZygoTaiga (C1)

Myrna (C2)

Phabba (C2)

Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
Plaque NotesPlaques are clear and vary in size from 1-2 mm
MorphotypeMyoviridae
Has been Phamerated?Yes
Gene List

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SmallFry_1

SmallFry_2

SmallFry_3
(nucleotidyltransferase)

SmallFry_4

SmallFry_5

SmallFry_6

SmallFry_7

SmallFry_8

SmallFry_9

SmallFry_10

SmallFry_11

SmallFry_12

SmallFry_13

SmallFry_14

SmallFry_15

SmallFry_16

SmallFry_17
(histidine triad nucleotide binding protein)

SmallFry_18

SmallFry_19

SmallFry_20

SmallFry_21

SmallFry_22

SmallFry_23

SmallFry_24

SmallFry_25

SmallFry_26

SmallFry_27

SmallFry_28

SmallFry_29

SmallFry_30

SmallFry_31
(helix-turn-helix DNA binding protein)

SmallFry_32

SmallFry_33

SmallFry_34

SmallFry_35
(ribbon-helix-helix DNA binding domain protein)

SmallFry_36

SmallFry_37

SmallFry_38

SmallFry_39

SmallFry_40

SmallFry_41

SmallFry_42

SmallFry_43

SmallFry_44

SmallFry_45

SmallFry_46

SmallFry_47

SmallFry_48

SmallFry_49

SmallFry_50

SmallFry_51

SmallFry_52

SmallFry_53

SmallFry_54

SmallFry_55
(helix-turn-helix DNA binding protein)

SmallFry_56

SmallFry_57

SmallFry_58

SmallFry_59

SmallFry_60

SmallFry_61

SmallFry_62
(helix-turn-helix DNA binding domain protein)

SmallFry_63
(helix-turn-helix DNA binding domain protein)

SmallFry_64

SmallFry_65

SmallFry_66

SmallFry_67

SmallFry_68

SmallFry_69

SmallFry_70

SmallFry_71

SmallFry_72

SmallFry_73

SmallFry_74

SmallFry_75

SmallFry_76

SmallFry_77

SmallFry_78

SmallFry_79

SmallFry_80

SmallFry_81

SmallFry_82

SmallFry_83

SmallFry_88
(methyltransferase)

SmallFry_89

SmallFry_90
(portal protein)

SmallFry_91

SmallFry_92

SmallFry_93
(ThyX-like thymidylate synthase)

SmallFry_94

SmallFry_95
(RNA ligase)

SmallFry_96

SmallFry_97
(LysM domain protein)

SmallFry_98

SmallFry_99

SmallFry_100
(major capsid protein)

SmallFry_101
(capsid decoration protein)

SmallFry_102

SmallFry_103

SmallFry_104

SmallFry_105

SmallFry_106

SmallFry_107

SmallFry_108
(membrane protein)

SmallFry_109

SmallFry_110

SmallFry_111

SmallFry_112

SmallFry_113

SmallFry_114

SmallFry_115

SmallFry_116

SmallFry_117

SmallFry_118

SmallFry_119

SmallFry_120

SmallFry_121
(N-acetyltransferase)

SmallFry_122

SmallFry_123

SmallFry_124

SmallFry_125

SmallFry_126

SmallFry_127
(tail sheath protein)

SmallFry_128

SmallFry_129

SmallFry_130
(tail assembly chaperone)

SmallFry_131
(tail assembly chaperone)

SmallFry_132
(tape measure protein)

SmallFry_133
(minor tail protein)

SmallFry_134

SmallFry_135

SmallFry_136
(minor tail protein)

SmallFry_137

SmallFry_138

SmallFry_139
(baseplate wedge protein)

SmallFry_140
(baseplate wedge protein)

SmallFry_141

SmallFry_142

SmallFry_143

SmallFry_144
(minor tail protein)

SmallFry_145
(minor tail protein)

SmallFry_146

SmallFry_147

SmallFry_148

SmallFry_149

SmallFry_150

SmallFry_151

SmallFry_152

SmallFry_153

SmallFry_157

SmallFry_158
(peptidyl tRNA hydrolase)

SmallFry_172
(HNH endonuclease)

SmallFry_174
(D-ala-D-ala carboxypeptidase)

SmallFry_175

SmallFry_187

SmallFry_188
(helicase)

SmallFry_189

SmallFry_190

SmallFry_191

SmallFry_192

SmallFry_193

SmallFry_194

SmallFry_195

SmallFry_196

SmallFry_197
(thymidylate kinase)

SmallFry_198
(aminotransferase)

SmallFry_199
(RNA binding domain protein)

SmallFry_200

SmallFry_201

SmallFry_202
(DnaC-like helicase loader)

SmallFry_203
(DnaB-like dsDNA helicase)

SmallFry_204

SmallFry_205
(DNA primase)

SmallFry_206

SmallFry_207
(DnaJ-like chaperonin)

SmallFry_208
(ssDNA binding domain protein)

SmallFry_209
(DNA polymerase III (alpha))

SmallFry_210
(Rf-1 peptide chain release factor)

SmallFry_211
(RecA-like DNA recombinase)

SmallFry_212
(holliday junction resolvase)

SmallFry_213

SmallFry_214
(HNH endonuclease)

SmallFry_215
(RusA-like resolvase)

SmallFry_216

SmallFry_217

SmallFry_218

SmallFry_219

SmallFry_220
(glycoside hydrolase)

SmallFry_221

SmallFry_222
(HNH endonuclease)

SmallFry_223

SmallFry_226

SmallFry_227

SmallFry_228

SmallFry_229

SmallFry_230

SmallFry_231

SmallFry_232

SmallFry_233

SmallFry_234
(Ro-like RNA binding protein)

SmallFry_235

SmallFry_236

SmallFry_237

SmallFry_238

SmallFry_239

SmallFry_240

SmallFry_241
(galactosaminyltransferase)

SmallFry_242

SmallFry_243
(glycosyltransferase)

SmallFry_244
(galactosyltransferase)

SmallFry_245
(nicotinate ribosyltransferase)

SmallFry_246
(glycosyltransferase)

SmallFry_247

SmallFry_248

SmallFry_249

SmallFry_250

SmallFry_251
(lysin A)

SmallFry_252
(membrane protein)

SmallFry_253
(lysin B)

SmallFry_254
(terminase)

SmallFry_255

SmallFry_256

SmallFry_257

SmallFry_258

SmallFry_259
(polynucleotide kinase)

SmallFry_260

SmallFry_261

SmallFry_262
(serine/threonine kinase)

SmallFry_263

SmallFry_264

SmallFry_265
(adenylosuccinate synthetase)

SmallFry_266

SmallFry_267

SmallFry_268

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK359311
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
SEA Designator 2012BRWNSmallFryNLD
SEA Lysate Titer 5.9 x 10^10
Date of SEA Lysate Titering Nov 14, 2012
Pitt Freezer Box# 33
Pitt Freezer Box Grid# 43
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload