Mycobacterium phage TinyTim
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Detailed Information for Phage TinyTim
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByHanna Legator
Year Found2010
Location FoundSt. Louis, MO USA
Finding InstitutionWashington University in St. Louis
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates38.645104 N, 90.31481 W Map
Discovery Notesdry soil surrounding large tree
20 feet outside of dorm on college campus
about 2 inches deep
outside temperature about 90 degrees F
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 20, 2011
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIon Torrent Sequencing
Approximate Shotgun Coverage44
Sanger Finishing Reactions13
Genome length (bp)153817
Character of genome endsCircularly Permuted
GC Content64.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Cluster Life CycleLytic
Other Cluster Members

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Boonesok (None)

CLach (None)

DavidMina (None)

DitchDweller (None)

Elpis (None)

Genet (None)

Hard2get (None)

Hippocrates (None)

Kali (None)

Linnaeus (None)

LittleSpot (None)

Lsquared (None)

Marlex (None)

Minipoke (None)

MoBama (None)

PeeWee (None)

Pesky7 (None)

Phomnie (None)

Phyllis (None)

Roku (None)

Sagamore (None)

Samarai (None)

ShenziUR (None)

SuperCallie (None)

TinyC (None)

Trilla23 (None)

Turret (None)

Squidney (A3)

Ading (C1)

Adlitam (C1)

Alice (C1)

Amataga (C1)

ArcherS7 (C1)

Astraea (C1)

Atlantean (C1)

Atlas (C1)

Audrick (C1)

Ava3 (C1)

BadAgartude (C1)

Bangla1971 (C1)

Basquiat (C1)

BeanWater (C1)

Bigswole (C1)

Bipolarisk (C1)

Bonray (C1)

Bread (C1)

Breeniome (C1)

Burrough (C1)

Bxz1 (C1)

Cali (C1)

Cane17 (C1)

Catera (C1)

Chargie21 (C1)

CharlieB (C1)

ChickenPhender (C1)

Colt (C1)

Daffodil (C1)

Dandelion (C1)

Darko (C1)

Delilah (C1)

Derek (C1)

Dietrick (C1)

Donegal (C1)

Doodle1 (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Exxus285 (C1)

Flabslab (C1)

Fludd (C1)

FoxtrotP1 (C1)

FrayBell (C1)

FudgeTart (C1)

Gabriel (C1)

Ghost (C1)

Gizmo (C1)

Grungle (C1)

Guwapp (C1)

HarrySpotter (C1)

HyRo (C1)

InigoMontoya (C1)

InterFolia (C1)

Iota (C1)

Isee1101 (C1)

JennanaJr (C1)

Jessibeth14 (C1)

JustHall (C1)

Kamryn (C1)

Khaleesi (C1)

Koguma (C1)

LifeSavor (C1)

LinStu (C1)

Lionheart (C1)

Littleton (C1)

Loanshark (C1)

LolaVinca (C1)

LRRHood (C1)

Lukilu (C1)

McWolfish (C1)

Megamind (C1)

Melpomini (C1)

MePac (C1)

MikeLiesIn (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

MrsHay (C1)

Naija (C1)

Napoleon13 (C1)

Nappy (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

OaklaforG (C1)

ParkTD (C1)

Patch (C1)

Patter (C1)

Phlegm (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

QBert (C1)

Rabinovish (C1)

Rahel (C1)

Rizal (C1)

RoMag (C1)

Roots515 (C1)

Salacia (C1)

SandySandal (C1)

Sauce (C1)

ScottMcG (C1)

Sebastian (C1)

Sebata (C1)

ShankPowerGod (C1)

Shaqnato (C1)

Shelob (C1)

ShiaLabeouf (C1)

Shnickers (C1)

Shrimp (C1)

SmallFry (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

Stubby (C1)

Teardrop (C1)

TheO (C1)

Thuathua (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Tyke (C1)

ValleyTerrace (C1)

Wally (C1)

Willis (C1)

Yucca (C1)

Zalkecks (C1)

Zeenon (C1)

ZygoTaiga (C1)

Matcha (C2)

Myrna (C2)

Phabba (C2)

SimSimmers (C2)

SrishMeg2525 (C2)

Annotation StatusIn GenBank
Plaque Notesvery small plaques
turbidity hard to verify because so small, but I think clear
MorphotypeMyoviridae
Has been Phamerated?Yes
Gene List

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TinyTim_1

TinyTim_2

TinyTim_3
(HNH endonuclease)

TinyTim_4

TinyTim_5

TinyTim_6

TinyTim_7

TinyTim_8

TinyTim_9

TinyTim_10

TinyTim_11

TinyTim_12

TinyTim_13

TinyTim_14

TinyTim_15

TinyTim_16

TinyTim_17

TinyTim_18
(histidine triad nucleotide domain protein)

TinyTim_19

TinyTim_20

TinyTim_21

TinyTim_22

TinyTim_23

TinyTim_24

TinyTim_25

TinyTim_26

TinyTim_27

TinyTim_28

TinyTim_29

TinyTim_30

TinyTim_31
(helix-turn-helix DNA binding protein)

TinyTim_32

TinyTim_33

TinyTim_34

TinyTim_35
(ribbon-helix-helix DNA binding domain)

TinyTim_36

TinyTim_37

TinyTim_38

TinyTim_39

TinyTim_40

TinyTim_41

TinyTim_42

TinyTim_43

TinyTim_44

TinyTim_45

TinyTim_46

TinyTim_47

TinyTim_48

TinyTim_49

TinyTim_50

TinyTim_51

TinyTim_52

TinyTim_53

TinyTim_54

TinyTim_55

TinyTim_56
(helix-turn-helix DNA binding protein)

TinyTim_57

TinyTim_58

TinyTim_59

TinyTim_60

TinyTim_61

TinyTim_62

TinyTim_63
(helix-turn-helix DNA binding domain)

TinyTim_64
(helix-turn-helix DNA binding domain)

TinyTim_65

TinyTim_66

TinyTim_67

TinyTim_68

TinyTim_69

TinyTim_70

TinyTim_71

TinyTim_72

TinyTim_73

TinyTim_74

TinyTim_75

TinyTim_76

TinyTim_77

TinyTim_78

TinyTim_79

TinyTim_80

TinyTim_81

TinyTim_82

TinyTim_83

TinyTim_88
(methyltransferase)

TinyTim_89

TinyTim_90
(portal protein)

TinyTim_91

TinyTim_92

TinyTim_93
(ThyX-like thymidylate synthase)

TinyTim_94

TinyTim_95
(RNA ligase)

TinyTim_96
(LysM-domain)

TinyTim_97

TinyTim_98

TinyTim_99
(major capsid protein)

TinyTim_100
(Holliday junction resolvase)

TinyTim_101
(capsid decoration protein)

TinyTim_102

TinyTim_103

TinyTim_104

TinyTim_105

TinyTim_106

TinyTim_107

TinyTim_108
(membrane protein)

TinyTim_109

TinyTim_110

TinyTim_111

TinyTim_112

TinyTim_113

TinyTim_114

TinyTim_115

TinyTim_116

TinyTim_117

TinyTim_118

TinyTim_119

TinyTim_120

TinyTim_121
(N-acetyltransferase)

TinyTim_122

TinyTim_123

TinyTim_124

TinyTim_125

TinyTim_126

TinyTim_127
(tail shealth protein)

TinyTim_128

TinyTim_129

TinyTim_130
(tail assembly chaperone)

TinyTim_131
(tail assembly chaperone)

TinyTim_132
(tape measure protein)

TinyTim_133

TinyTim_134

TinyTim_135

TinyTim_136
(minor tail protein)

TinyTim_137

TinyTim_138

TinyTim_139
(baseplate wedge protein)

TinyTim_140
(baseplate wedge protein)

TinyTim_141

TinyTim_142

TinyTim_143

TinyTim_144
(minor tail protein)

TinyTim_145
(minor tail protein)

TinyTim_146

TinyTim_147

TinyTim_148

TinyTim_149

TinyTim_150

TinyTim_151

TinyTim_152

TinyTim_156

TinyTim_157
(peptidyl tRNA hydrolase)

TinyTim_172
(minor tail protein, D-ala-D-ala carboxypeptidase)

TinyTim_173

TinyTim_185

TinyTim_186
(helicase)

TinyTim_187

TinyTim_188

TinyTim_189

TinyTim_190

TinyTim_191

TinyTim_192

TinyTim_193

TinyTim_194

TinyTim_195

TinyTim_196
(thymidylate kinase)

TinyTim_197
(aminotransferase)

TinyTim_198
(RNA binding domain)

TinyTim_199

TinyTim_200
(DnaC-like helicase loader)

TinyTim_201
(DnaB-like dsDNA helicase)

TinyTim_202

TinyTim_203
(DNA primase)

TinyTim_204

TinyTim_205
(chaperonin DnaJ-like)

TinyTim_206

TinyTim_207
(DNA polymerase III (alpha))

TinyTim_208
(RF-1 peptide chain release factor)

TinyTim_209
(RecA-like DNA recombinase)

TinyTim_210
(Holliday junction revolvase)

TinyTim_211

TinyTim_212
(HNH endonuclease)

TinyTim_213
(RusA-like revolvase)

TinyTim_214

TinyTim_215

TinyTim_216

TinyTim_217

TinyTim_218
(glycloside hydrolase)

TinyTim_219

TinyTim_220

TinyTim_223

TinyTim_224

TinyTim_225

TinyTim_226

TinyTim_227

TinyTim_228

TinyTim_229

TinyTim_230

TinyTim_231
(Ro-like RNA binding protein)

TinyTim_232

TinyTim_233

TinyTim_234

TinyTim_235

TinyTim_236

TinyTim_237

TinyTim_238
(galactosaminyltransferase)

TinyTim_239

TinyTim_240
(glycosyltransferase)

TinyTim_241
(galactosyltransferase)

TinyTim_242
(nicotinate ribosyltransferase)

TinyTim_243
(glycosyltransferase)

TinyTim_244

TinyTim_245

TinyTim_246

TinyTim_247

TinyTim_248
(HNH endonuclease)

TinyTim_249
(lysin A)

TinyTim_250
(membrane protein)

TinyTim_251
(lysin B)

TinyTim_252
(terminase)

TinyTim_253

TinyTim_254

TinyTim_255

TinyTim_256

TinyTim_257
(polynucleotide kinase)

TinyTim_258

TinyTim_259
(serine/threonine kinase)

TinyTim_260

TinyTim_261
(adenylosuccinate synthetase)

TinyTim_262

TinyTim_263

TinyTim_264

TinyTim_265

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK112554
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
SEA Designator WUSL
SEA Lysate Titer 4.23e10
Date of SEA Lysate Titering Sep 30, 2010
Pitt Freezer Box# 20
Pitt Freezer Box Grid# 35
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload