Mycobacterium phage Ronan
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Detailed Information for Phage Ronan
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByRichard Van + William Nie
Year Found2019
Location FoundLas Vegas, NV United States
Finding InstitutionUniversity of Nevada Las Vegas
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature36°C
GPS Coordinates36.113611 N, 115.144444 W Map
Discovery NotesThe environmental sample was collected from a garden bed at the UNLV community garden. The soil had a black/brown color and came from an area with partial shading. The time of collection was 10:31am on 9/3/19. The the ambient temperature was 96ºF, humidity was 23%, and wind speed was 4mph.
Naming NotesInitially, the phage was supposed to be an anagram of the UTA's that gave us the adopted plate. It has since evolved into the name "Ronan" after the villain in Guardians of the Galaxy.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 23, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage273
Genome length (bp)154852
Character of genome endsCircularly Permuted
GC Content64.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Cluster Life CycleLytic
Lysogeny NotesPlaques were turbid throughout the isolation/purification process.
Other Cluster Members

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Ading (C1)

Adlitam (C1)

Alice (C1)

Amataga (C1)

ArcherS7 (C1)

Astraea (C1)

Atlantean (C1)

Audrick (C1)

Ava3 (C1)

BackyardAgain (C1)

BadAgartude (C1)

BananaFence (C1)

Bangla1971 (C1)

Basquiat (C1)

BeanWater (C1)

Bigswole (C1)

Bipolarisk (C1)

Blackbrain (C1)

Bonray (C1)

Bread (C1)

Breeniome (C1)

Burrough (C1)

Bxz1 (C1)

Cactojaque (C1)

Cali (C1)

Cane17 (C1)

Catera (C1)

Chargie21 (C1)

CharlieB (C1)

ChaylaJr (C1)

ChickenPhender (C1)

Colt (C1)

Daffodil (C1)

Dandelion (C1)

Darko (C1)

Delilah (C1)

Derek (C1)

Dietrick (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EasyJones (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Flabslab (C1)

Fludd (C1)

FoxtrotP1 (C1)

FrayBell (C1)

FudgeTart (C1)

Gabriel (C1)

Ghost (C1)

Gizmo (C1)

Grungle (C1)

Guwapp (C1)

HyRo (C1)

InigoMontoya (C1)

InterFolia (C1)

Iota (C1)

Janiyra (C1)

Jaygup (C1)

Jessibeth14 (C1)

JPickles (C1)

JustHall (C1)

Kamryn (C1)

Kboogie (C1)

Khaleesi (C1)

Koguma (C1)

Latch (C1)

LifeSavor (C1)

LinStu (C1)

Littleton (C1)

Loanshark (C1)

LolaVinca (C1)

LRRHood (C1)

Lukilu (C1)

Mangeria (C1)

McWolfish (C1)

Megamind (C1)

Melpomini (C1)

MikeLiesIn (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

Naija (C1)

Napoleon13 (C1)

Nappy (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

ParkTD (C1)

Patter (C1)

Phlegm (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pinkcreek (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

QBert (C1)

Quasimodo (C1)

Rabinovish (C1)

Rahel (C1)

Rizal (C1)

RoMag (C1)

Ronan (C1)

Roots515 (C1)

Salacia (C1)

Sauce (C1)

ScottMcG (C1)

Sebata (C1)

Shaqnato (C1)

Shelob (C1)

ShiaLabeouf (C1)

Shifa (C1)

Shnickers (C1)

Shrimp (C1)

SmallFry (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

StephanieG (C1)

Stubby (C1)

Teardrop (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Trinitium (C1)

Turret (C1)

Tyke (C1)

ValleyTerrace (C1)

Wally (C1)

Willis (C1)

YoungMoneyMata (C1)

Yucca (C1)

Zalkecks (C1)

Zeenon (C1)

ZygoTaiga (C1)

Myrna (C2)

Phabba (C2)

Annotating InstitutionUniversity of Nevada Las Vegas
Annotation StatusIn GenBank
Plaque NotesRound with irregular margin, plaque size varies from 0.3mm to 1.0mm, turbid.
MorphotypeMyoviridae
Has been Phamerated?Yes
Gene List

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Ronan_1

Ronan_2

Ronan_3
(nucleotidyl transferase)

Ronan_4

Ronan_5

Ronan_6

Ronan_7

Ronan_8

Ronan_9

Ronan_10

Ronan_11

Ronan_12

Ronan_13

Ronan_14

Ronan_15

Ronan_16

Ronan_17

Ronan_18

Ronan_19

Ronan_20

Ronan_21

Ronan_22

Ronan_23

Ronan_24
(band-7-like membrane protein)

Ronan_25

Ronan_26
(helix-turn-helix DNA binding domain protein)

Ronan_27

Ronan_28

Ronan_29

Ronan_30
(ribbon-helix-helix DNA binding domain protein)

Ronan_31

Ronan_32

Ronan_33

Ronan_34

Ronan_35

Ronan_36

Ronan_37

Ronan_38

Ronan_39

Ronan_40
(helix-turn-helix DNA binding domain protein)

Ronan_41
(immunity repressor)

Ronan_42

Ronan_43

Ronan_44

Ronan_45

Ronan_46

Ronan_47

Ronan_48

Ronan_49

Ronan_50
(helix-turn-helix DNA binding domain protein)

Ronan_51

Ronan_52

Ronan_53

Ronan_54

Ronan_55

Ronan_56

Ronan_57
(helix-turn-helix DNA binding domain protein)

Ronan_58
(helix-turn-helix DNA binding domain protein)

Ronan_59

Ronan_60

Ronan_61

Ronan_62

Ronan_63

Ronan_64

Ronan_65

Ronan_66

Ronan_67

Ronan_68

Ronan_69

Ronan_70

Ronan_71

Ronan_72

Ronan_73

Ronan_74

Ronan_75

Ronan_76
(ParB-like nuclease domain)

Ronan_81
(methyltransferase)

Ronan_82

Ronan_83
(portal protein)

Ronan_84

Ronan_85

Ronan_86
(ThyX-like thymidylate synthase)

Ronan_87

Ronan_88
(RNA ligase)

Ronan_89
(HNH endonuclease)

Ronan_90
(LysM-like peptidoglycan binding protein)

Ronan_91

Ronan_92

Ronan_93
(major capsid protein)

Ronan_94
(holliday junction resolvase)

Ronan_95
(capsid decoration protein)

Ronan_96

Ronan_97

Ronan_98

Ronan_99

Ronan_100

Ronan_101

Ronan_102

Ronan_103

Ronan_104

Ronan_105

Ronan_106

Ronan_107

Ronan_108

Ronan_109

Ronan_110

Ronan_111
(peptidyl tRNA hydrolase)

Ronan_112

Ronan_113

Ronan_114

Ronan_115
(N-acetyltransferase)

Ronan_116

Ronan_117

Ronan_118

Ronan_119

Ronan_120

Ronan_121
(tail sheath protein)

Ronan_122
(tail tube protein)

Ronan_123

Ronan_124
(tail assembly chaperone)

Ronan_125
(tail assembly chaperone)

Ronan_126
(tape measure protein)

Ronan_127
(minor tail protein)

Ronan_128

Ronan_129

Ronan_130
(minor tail protein)

Ronan_131

Ronan_132
(minor tail protein)

Ronan_133
(baseplate wedge protein)

Ronan_134
(baseplate J protein)

Ronan_135

Ronan_136

Ronan_137

Ronan_138
(minor tail protein)

Ronan_139
(minor tail protein)

Ronan_140

Ronan_141

Ronan_142

Ronan_143

Ronan_144

Ronan_145

Ronan_149

Ronan_150

Ronan_151
(peptidyl tRNA hydrolase)

Ronan_158

Ronan_160

Ronan_169
(minor tail protein)

Ronan_170

Ronan_171

Ronan_182

Ronan_183
(helicase)

Ronan_184

Ronan_185

Ronan_186

Ronan_187

Ronan_188

Ronan_189

Ronan_190

Ronan_191

Ronan_192

Ronan_193
(thymidylate kinase)

Ronan_194
(aminotransferase)

Ronan_195
(RNA binding protein)

Ronan_196

Ronan_197

Ronan_198
(DnaC-like helicase loader)

Ronan_199
(DnaB-like dsDNA helicase)

Ronan_200

Ronan_201
(DNA primase)

Ronan_202

Ronan_203
(DnaJ-like chaperonin)

Ronan_204
(ssDNA binding protein)

Ronan_205
(DnaE-like DNA polymerase III)

Ronan_206
(RF-1 peptide chain release factor)

Ronan_207
(RecA-like DNA recombinase)

Ronan_208
(holliday junction resolvase)

Ronan_209

Ronan_210
(HNH endonuclease)

Ronan_211
(RusA-like resolvase)

Ronan_212

Ronan_213

Ronan_214

Ronan_215

Ronan_216
(glycoside hydrolase)

Ronan_217

Ronan_218

Ronan_221

Ronan_222

Ronan_223

Ronan_224

Ronan_225

Ronan_226

Ronan_227

Ronan_228

Ronan_229
(Ro-like RNA binding protein)

Ronan_230

Ronan_231

Ronan_232

Ronan_233

Ronan_234

Ronan_235

Ronan_236
(glycosyltransferase)

Ronan_237

Ronan_238
(glycosyltransferase)

Ronan_239
(galactosyltransferase)

Ronan_240
(nicotinate ribosyltransferase)

Ronan_241
(glycosyltransferase)

Ronan_242

Ronan_243

Ronan_244

Ronan_245

Ronan_246
(lysin A, N-acetylmuramoyl-L-alanine amidase domain)

Ronan_247
(holin)

Ronan_248
(lysin B)

Ronan_249
(terminase)

Ronan_250

Ronan_251

Ronan_252

Ronan_253

Ronan_254
(polynucleotide kinase)

Ronan_255

Ronan_256

Ronan_257
(serine/threonine kinase)

Ronan_258

Ronan_259

Ronan_260
(PurA-like adenylosuccinate synthetase)

Ronan_261

Ronan_262

Ronan_263

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMT553335
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 97
Pitt Freezer Box Grid# G12
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
GenBank File for PhameratorDownload