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Dre3 (CT) Misfit (E) Indigenous (BI1) Bexan (A1) Danforth (A8)
Mycobacterium phage Melpomini
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Detailed Information for Phage Melpomini
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByYevgeniya Miglo
Year Found2014
Location FoundBaltimore, MD USA
Finding InstitutionJohns Hopkins University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates39.328399 N, 76.618048 W Map
Discovery NotesObtained: 8/27/2014 2:20pm 88 degrees f
3 cm in depth
dry
urban
across the street from buildings
foot traffic about 4 meters away
Charles Street about 5 meters away
under a tree - indirect sunlight
on a slope
Naming NotesNamed after Melpomene, after the greek muse of tragedy.
Because of the small tail, "mene" was changed to "mini."
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 18, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1658
Genome length (bp)154855
Character of genome endsCircularly Permuted
GC Content64.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Cluster Life CycleLytic
Other Cluster Members

Click to View

Ading (C1)

Adlitam (C1)

Alice (C1)

Amataga (C1)

ArcherS7 (C1)

Astraea (C1)

Atlantean (C1)

Audrick (C1)

Ava3 (C1)

BadAgartude (C1)

Bangla1971 (C1)

Basquiat (C1)

BeanWater (C1)

Bigswole (C1)

Bipolarisk (C1)

Blackbrain (C1)

Bonray (C1)

Bread (C1)

Breeniome (C1)

Burrough (C1)

Bxz1 (C1)

Cactojaque (C1)

Cali (C1)

Cane17 (C1)

Catera (C1)

Chargie21 (C1)

CharlieB (C1)

ChickenPhender (C1)

Colt (C1)

Daffodil (C1)

Dandelion (C1)

Darko (C1)

Delilah (C1)

Derek (C1)

Dietrick (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Flabslab (C1)

Fludd (C1)

FoxtrotP1 (C1)

FrayBell (C1)

FudgeTart (C1)

Gabriel (C1)

Ghost (C1)

Gizmo (C1)

Grungle (C1)

Guwapp (C1)

HyRo (C1)

InigoMontoya (C1)

InterFolia (C1)

Iota (C1)

Jessibeth14 (C1)

JPickles (C1)

JustHall (C1)

Kamryn (C1)

Kboogie (C1)

Khaleesi (C1)

Koguma (C1)

LifeSavor (C1)

LinStu (C1)

Littleton (C1)

Loanshark (C1)

LolaVinca (C1)

LRRHood (C1)

Lukilu (C1)

Mangeria (C1)

McWolfish (C1)

Megamind (C1)

Melpomini (C1)

MikeLiesIn (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

Naija (C1)

Napoleon13 (C1)

Nappy (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

ParkTD (C1)

Patter (C1)

Phlegm (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pinkcreek (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

QBert (C1)

Rabinovish (C1)

Rahel (C1)

Rizal (C1)

RoMag (C1)

Roots515 (C1)

Salacia (C1)

Sauce (C1)

ScottMcG (C1)

Sebata (C1)

Shaqnato (C1)

Shelob (C1)

ShiaLabeouf (C1)

Shifa (C1)

Shnickers (C1)

Shrimp (C1)

SmallFry (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

StephanieG (C1)

Stubby (C1)

Teardrop (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Trinitium (C1)

Tyke (C1)

ValleyTerrace (C1)

Wally (C1)

Willis (C1)

YoungMoneyMata (C1)

Yucca (C1)

Zalkecks (C1)

Zeenon (C1)

ZygoTaiga (C1)

Myrna (C2)

Phabba (C2)

Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
Plaque Notes1-2 mm clear plaques at 48 hours
MorphotypeMyoviridae
Has been Phamerated?Yes
Gene List

Click to View

Melpomini_1

Melpomini_2

Melpomini_3
(nucleotidyltransferase)

Melpomini_4

Melpomini_5

Melpomini_6

Melpomini_7

Melpomini_8

Melpomini_9

Melpomini_10

Melpomini_11

Melpomini_12

Melpomini_13

Melpomini_14

Melpomini_15

Melpomini_16

Melpomini_17
(histidine triad nucleotide binding protein)

Melpomini_18

Melpomini_19

Melpomini_20

Melpomini_21

Melpomini_22

Melpomini_23

Melpomini_24

Melpomini_25

Melpomini_26

Melpomini_27

Melpomini_28

Melpomini_29

Melpomini_30
(helix-turn-helix DNA binding protein)

Melpomini_31

Melpomini_32

Melpomini_33

Melpomini_34

Melpomini_35

Melpomini_36

Melpomini_37

Melpomini_38

Melpomini_39

Melpomini_40

Melpomini_41

Melpomini_42

Melpomini_43

Melpomini_44

Melpomini_45

Melpomini_46

Melpomini_47

Melpomini_48

Melpomini_49

Melpomini_50

Melpomini_51
(helix-turn-helix DNA binding protein)

Melpomini_52

Melpomini_53

Melpomini_54

Melpomini_55

Melpomini_56

Melpomini_57

Melpomini_58
(helix-turn-helix DNA binding domain protein)

Melpomini_59
(helix-turn-helix DNA binding domain protein)

Melpomini_60

Melpomini_61

Melpomini_62

Melpomini_63

Melpomini_64

Melpomini_65

Melpomini_66

Melpomini_67

Melpomini_68

Melpomini_69

Melpomini_70

Melpomini_71

Melpomini_72

Melpomini_73

Melpomini_74

Melpomini_75

Melpomini_76

Melpomini_77

Melpomini_82
(methyltransferase)

Melpomini_83

Melpomini_84
(portal protein)

Melpomini_85

Melpomini_86

Melpomini_87
(ThyX-like thymidylate synthase)

Melpomini_88

Melpomini_89
(RNA ligase)

Melpomini_90
(LysM-domain protein)

Melpomini_91

Melpomini_92

Melpomini_93
(major capsid protein)

Melpomini_94
(holliday junction resolvase)

Melpomini_95
(capsid decoration protein)

Melpomini_96

Melpomini_97

Melpomini_98

Melpomini_99

Melpomini_100

Melpomini_101

Melpomini_102
(membrane protein)

Melpomini_103

Melpomini_104

Melpomini_105

Melpomini_106

Melpomini_107

Melpomini_108

Melpomini_109

Melpomini_110

Melpomini_111

Melpomini_112

Melpomini_113

Melpomini_114

Melpomini_115
(N-acetyltransferase)

Melpomini_116

Melpomini_117

Melpomini_118

Melpomini_119

Melpomini_120

Melpomini_121
(tail sheath protein)

Melpomini_122

Melpomini_123

Melpomini_124
(tail assembly chaperone)

Melpomini_125
(tail assembly chaperone)

Melpomini_126
(tape measure protein)

Melpomini_127
(minor tail protein)

Melpomini_128

Melpomini_129

Melpomini_130
(minor tail protein)

Melpomini_131

Melpomini_132

Melpomini_133
(baseplate wedge protein)

Melpomini_134
(baseplate wedge protein)

Melpomini_135

Melpomini_136

Melpomini_137

Melpomini_138
(minor tail protein)

Melpomini_139
(minor tail protein)

Melpomini_140

Melpomini_141

Melpomini_142

Melpomini_143

Melpomini_144

Melpomini_145

Melpomini_146

Melpomini_147

Melpomini_150

Melpomini_151
(peptidyl tRNA hydrolase)

Melpomini_165
(HNH endonuclease)

Melpomini_167
(D-ala-D-ala carboxypeptidase)

Melpomini_168

Melpomini_180

Melpomini_181

Melpomini_182
(helicase)

Melpomini_183

Melpomini_184

Melpomini_185

Melpomini_186

Melpomini_187

Melpomini_188

Melpomini_189

Melpomini_190

Melpomini_191

Melpomini_192
(thymidylate kinase)

Melpomini_193
(aminotransferase)

Melpomini_194
(RNA binding domain protein)

Melpomini_195

Melpomini_196

Melpomini_197
(DnaC-like helicase loader)

Melpomini_198
(DnaB-like dsDNA helicase)

Melpomini_199

Melpomini_200
(DNA primase)

Melpomini_201

Melpomini_202
(DnaJ-like chaperonin)

Melpomini_203
(ssDNA binding domain protein)

Melpomini_204
(DNA polymerase III (alpha))

Melpomini_205
(RF-1 peptide chain release factor)

Melpomini_206
(RecA-like DNA recombinase)

Melpomini_207
(holliday junction resolvase)

Melpomini_208

Melpomini_209
(HNH endonuclease)

Melpomini_210
(RusA-like revolvase)

Melpomini_211

Melpomini_212

Melpomini_213

Melpomini_214

Melpomini_215
(glycoside hydrolase)

Melpomini_216

Melpomini_217

Melpomini_220

Melpomini_221

Melpomini_222

Melpomini_223

Melpomini_224

Melpomini_225

Melpomini_226

Melpomini_227

Melpomini_228
(Ro-like RNA binding protein)

Melpomini_229

Melpomini_230

Melpomini_231

Melpomini_232

Melpomini_233

Melpomini_234

Melpomini_235
(galactosaminyltransferase)

Melpomini_236

Melpomini_237
(glycosyltransferase)

Melpomini_238
(galactosyltransferase)

Melpomini_239
(nicotinate ribosyltransferase)

Melpomini_240
(glycosyltransferase)

Melpomini_241

Melpomini_242

Melpomini_243

Melpomini_244

Melpomini_245
(lysin A)

Melpomini_246
(membrane protein)

Melpomini_247
(lysin B)

Melpomini_248
(terminase)

Melpomini_249

Melpomini_250

Melpomini_251

Melpomini_252

Melpomini_253
(polynucleotide kinase)

Melpomini_254

Melpomini_255
(serine/threonine kinase)

Melpomini_256

Melpomini_257

Melpomini_258
(adenylosuccinate synthetase)

Melpomini_259

Melpomini_260

Melpomini_261

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK450424
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.8 x 10^11 pfu/mL
Date of SEA Lysate Titering Oct 23, 2014
Pitt Freezer Box# 14
Pitt Freezer Box Grid# A10
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload