Mycobacterium phage Grungle
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Detailed Information for Phage Grungle
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByMelissa Folkerts
Year Found2018
Location FoundLos Angeles, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates34.066687 N, 118.451216 W Map
Discovery NotesPhage Grungle was isolated from Damp, dark, rich soil collected on April 11, 2018 by Melissa Folkerts at a depth of about 4 inches. Air temp at time of collection was 16.7˚C. Grungle was isolated by direct plating at 30˚C.

Characterization was performed by Team Plaque Donald's Big Plaques (Richmund Tan, Melissa Folkerts, Lovelyn Edillo, Neiki Rokni), Team B.KFC (Kimberly Uehisa, Brooke Crosswhite, Christopher Huerta, Fangkun Li), and Team KARL (Karina Haleblian, Alexis Ballew, Lillian Lai, Rinah Kim).

103BL-S18 Instructor: Dr. Amanda Freise
Teaching Assistant: Samuel Wu
Undergraduate Assistants: Mariam Khachatrian, Kalin Patel
Laboratory Support: Dr. Krisanavane Reddi and Dr. Jordan Moberg Parker
Naming NotesOne of PDBP's group members read a story about a person having a dream that they were at Hogwarts and the sorting hat grouped them into "Grunglebunk." This inspired the name Grungle.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 3, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage527
Genome length (bp)153739
Character of genome endsCircularly Permuted
GC Content64.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Cluster Life CycleLytic
Other Cluster Members

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Ading (C1)

Adlitam (C1)

Alice (C1)

Amataga (C1)

ArcherS7 (C1)

Astraea (C1)

Atlantean (C1)

Audrick (C1)

Ava3 (C1)

BadAgartude (C1)

Bangla1971 (C1)

Basquiat (C1)

BeanWater (C1)

Bigswole (C1)

Bipolarisk (C1)

Blackbrain (C1)

Bonray (C1)

Bread (C1)

Breeniome (C1)

Burrough (C1)

Bxz1 (C1)

Cactojaque (C1)

Cali (C1)

Cane17 (C1)

Catera (C1)

Chargie21 (C1)

CharlieB (C1)

ChaylaJr (C1)

ChickenPhender (C1)

Colt (C1)

Daffodil (C1)

Dandelion (C1)

Darko (C1)

Delilah (C1)

Derek (C1)

Dietrick (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Flabslab (C1)

Fludd (C1)

FoxtrotP1 (C1)

FrayBell (C1)

FudgeTart (C1)

Gabriel (C1)

Ghost (C1)

Gizmo (C1)

Grungle (C1)

Guwapp (C1)

HyRo (C1)

InigoMontoya (C1)

InterFolia (C1)

Iota (C1)

Janiyra (C1)

Jessibeth14 (C1)

JPickles (C1)

JustHall (C1)

Kamryn (C1)

Kboogie (C1)

Khaleesi (C1)

Koguma (C1)

LifeSavor (C1)

LinStu (C1)

Littleton (C1)

Loanshark (C1)

LolaVinca (C1)

LRRHood (C1)

Lukilu (C1)

Mangeria (C1)

McWolfish (C1)

Megamind (C1)

Melpomini (C1)

MikeLiesIn (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

Naija (C1)

Napoleon13 (C1)

Nappy (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

ParkTD (C1)

Patter (C1)

Phlegm (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pinkcreek (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

QBert (C1)

Quasimodo (C1)

Rabinovish (C1)

Rahel (C1)

Rizal (C1)

RoMag (C1)

Roots515 (C1)

Salacia (C1)

Sauce (C1)

ScottMcG (C1)

Sebata (C1)

Shaqnato (C1)

Shelob (C1)

ShiaLabeouf (C1)

Shifa (C1)

Shnickers (C1)

Shrimp (C1)

SmallFry (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

StephanieG (C1)

Stubby (C1)

Teardrop (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Trinitium (C1)

Tyke (C1)

ValleyTerrace (C1)

Wally (C1)

Willis (C1)

YoungMoneyMata (C1)

Yucca (C1)

Zalkecks (C1)

Zeenon (C1)

ZygoTaiga (C1)

Myrna (C2)

Phabba (C2)

Annotating InstitutionUniversity of California, Los Angeles
Annotation StatusIn GenBank
Plaque NotesPlaque morphology: clear
Average sizesize range diameter: 1.5 - 2 mm
MorphotypeMyoviridae
Has been Phamerated?Yes
Gene List

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Grungle_1

Grungle_2
(membrane protein)

Grungle_3
(nucleotidyl transferase)

Grungle_4

Grungle_5

Grungle_6

Grungle_7

Grungle_8

Grungle_9

Grungle_10

Grungle_11

Grungle_12

Grungle_13

Grungle_14

Grungle_15

Grungle_16

Grungle_17

Grungle_18

Grungle_19

Grungle_20

Grungle_21

Grungle_22
(band-7-like membrane protein)

Grungle_23

Grungle_24
(helix-turn-helix DNA binding domain protein)

Grungle_25

Grungle_26

Grungle_27

Grungle_28

Grungle_29

Grungle_30

Grungle_31

Grungle_32

Grungle_33

Grungle_34

Grungle_35

Grungle_36

Grungle_37

Grungle_38
(helix-turn-helix DNA binding domain protein)

Grungle_39
(immunity repressor)

Grungle_40

Grungle_41

Grungle_42

Grungle_43

Grungle_44

Grungle_45

Grungle_46

Grungle_47
(helix-turn-helix DNA binding domain protein)

Grungle_48

Grungle_49

Grungle_50

Grungle_51

Grungle_52

Grungle_53

Grungle_54
(helix-turn-helix DNA binding domain protein)

Grungle_55
(helix-turn-helix DNA binding domain protein)

Grungle_56

Grungle_57

Grungle_58

Grungle_59

Grungle_60

Grungle_61

Grungle_62

Grungle_63

Grungle_64

Grungle_65

Grungle_66

Grungle_67

Grungle_68

Grungle_69

Grungle_70

Grungle_71
(ParB-like nuclease domain protein)

Grungle_75

Grungle_76

Grungle_77
(portal protein)

Grungle_78

Grungle_79

Grungle_80
(ThyX-like thymidylate synthase)

Grungle_81

Grungle_82
(RNA ligase)

Grungle_83
(HNH endonuclease)

Grungle_84
(LysM-like endolysin)

Grungle_85
(protease)

Grungle_86

Grungle_87
(major capsid protein)

Grungle_88
(holliday junction resolvase)

Grungle_89

Grungle_90

Grungle_91

Grungle_92

Grungle_93

Grungle_94

Grungle_95

Grungle_96
(membrane protein)

Grungle_97

Grungle_98

Grungle_99

Grungle_100

Grungle_101

Grungle_102
(capsid decoration protein)

Grungle_103

Grungle_104

Grungle_105

Grungle_106

Grungle_107

Grungle_108

Grungle_109

Grungle_110

Grungle_111

Grungle_112

Grungle_113

Grungle_114
(tail sheath protein)

Grungle_115

Grungle_116

Grungle_117
(tail assembly chaperone)

Grungle_118
(tail assembly chaperone)

Grungle_119
(tape measure protein)

Grungle_120

Grungle_121

Grungle_122

Grungle_123
(minor tail protein)

Grungle_124

Grungle_125
(minor tail protein)

Grungle_126
(baseplate wedge protein)

Grungle_127
(baseplate J protein)

Grungle_128

Grungle_129

Grungle_130

Grungle_131
(minor tail protein)

Grungle_132
(minor tail protein)

Grungle_133

Grungle_134

Grungle_135

Grungle_136

Grungle_137

Grungle_138

Grungle_139

Grungle_142

Grungle_143
(peptidyl tRNA hydrolase)

Grungle_149
(LAGLIDADG endonuclease)

Grungle_159
(minor tail protein)

Grungle_160

Grungle_172

Grungle_173
(DNA helicase)

Grungle_174

Grungle_175

Grungle_176

Grungle_177

Grungle_178

Grungle_179

Grungle_180

Grungle_181

Grungle_182
(thymidylate kinase)

Grungle_183
(aminotransferase)

Grungle_184

Grungle_185

Grungle_186
(DnaC-like helicase loader)

Grungle_187
(DnaB-like dsDNA helicase)

Grungle_188

Grungle_189
(DNA primase)

Grungle_190

Grungle_191
(DnaJ-like chaperonin)

Grungle_192

Grungle_193
(DnaE-like DNA polymerase III)

Grungle_194
(RF-1 peptide chain release factor)

Grungle_195
(RecA-like DNA recombinase)

Grungle_196
(holliday junction resolvase)

Grungle_197

Grungle_198
(HNH endonuclease)

Grungle_199
(RusA-like resolvase)

Grungle_200

Grungle_201

Grungle_202

Grungle_203

Grungle_204

Grungle_205

Grungle_206

Grungle_209

Grungle_210

Grungle_211

Grungle_212

Grungle_213

Grungle_214

Grungle_215
(Ro-like RNA binding protein)

Grungle_216

Grungle_217

Grungle_218

Grungle_219

Grungle_220

Grungle_221

Grungle_222
(glycosyltransferase)

Grungle_223
(membrane protein)

Grungle_224
(glycosyltransferase)

Grungle_225
(glycosyltransferase)

Grungle_226
(nicotinate ribosyltransferase)

Grungle_227
(glycosyltransferase)

Grungle_228

Grungle_229

Grungle_230

Grungle_231

Grungle_232
(lysin A)

Grungle_233
(holin)

Grungle_234
(lysin B)

Grungle_235
(terminase)

Grungle_236

Grungle_237

Grungle_238

Grungle_239

Grungle_240
(polynucleotide kinase)

Grungle_241

Grungle_242

Grungle_243
(serine/threonine kinase)

Grungle_244
(HNH endonuclease)

Grungle_245

Grungle_246
(PurA-like adenylosuccinate synthetase)

Grungle_247

Grungle_248

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMN062707
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 78
Pitt Freezer Box Grid# C4
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload