Recently Added Phages

Khoblack Moturi Halokelier Tweety19 Divine

Recently Modified Phages

Zuko ZygoTaiga Zulu Zelda Zombie

Recently Finished Phages

Dre3 (CT) Misfit (E) Indigenous (BI1) Bexan (A1) Danforth (A8)
Mycobacterium phage McWolfish
Know something about this phage that we don't? Modify its data.
Detailed Information for Phage McWolfish
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesLysocool
Found ByLucas McClish & Tony Wolfe
Year Found2014
Location FoundColumbus, OH USA
Finding InstitutionThe Ohio State University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature25°C
GPS Coordinates39.999508 N, 83.012663 W Map
Discovery NotesEnrichment plates were obtained from other classmates cultures due to lack of phage from soil sample. Agarose gel electrophoresis produced what appeared to be unique DNA.
Naming NotesIt's a play of words off of lysosomes.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 15, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage719
Genome length (bp)154462
Character of genome endsCircularly Permuted
GC Content64.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Cluster Life CycleLytic
Other Cluster Members

Click to View

Ading (C1)

Adlitam (C1)

Alice (C1)

Amataga (C1)

ArcherS7 (C1)

Astraea (C1)

Atlantean (C1)

Audrick (C1)

Ava3 (C1)

BadAgartude (C1)

Bangla1971 (C1)

Basquiat (C1)

BeanWater (C1)

Bigswole (C1)

Bipolarisk (C1)

Blackbrain (C1)

Bonray (C1)

Bread (C1)

Breeniome (C1)

Burrough (C1)

Bxz1 (C1)

Cactojaque (C1)

Cali (C1)

Cane17 (C1)

Catera (C1)

Chargie21 (C1)

CharlieB (C1)

ChickenPhender (C1)

Colt (C1)

Daffodil (C1)

Dandelion (C1)

Darko (C1)

Delilah (C1)

Derek (C1)

Dietrick (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Flabslab (C1)

Fludd (C1)

FoxtrotP1 (C1)

FrayBell (C1)

FudgeTart (C1)

Gabriel (C1)

Ghost (C1)

Gizmo (C1)

Grungle (C1)

Guwapp (C1)

HyRo (C1)

InigoMontoya (C1)

InterFolia (C1)

Iota (C1)

Jessibeth14 (C1)

JPickles (C1)

JustHall (C1)

Kamryn (C1)

Kboogie (C1)

Khaleesi (C1)

Koguma (C1)

LifeSavor (C1)

LinStu (C1)

Littleton (C1)

Loanshark (C1)

LolaVinca (C1)

LRRHood (C1)

Lukilu (C1)

Mangeria (C1)

McWolfish (C1)

Megamind (C1)

Melpomini (C1)

MikeLiesIn (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

Naija (C1)

Napoleon13 (C1)

Nappy (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

ParkTD (C1)

Patter (C1)

Phlegm (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pinkcreek (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

QBert (C1)

Rabinovish (C1)

Rahel (C1)

Rizal (C1)

RoMag (C1)

Roots515 (C1)

Salacia (C1)

Sauce (C1)

ScottMcG (C1)

Sebata (C1)

Shaqnato (C1)

Shelob (C1)

ShiaLabeouf (C1)

Shifa (C1)

Shnickers (C1)

Shrimp (C1)

SmallFry (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

StephanieG (C1)

Stubby (C1)

Teardrop (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Trinitium (C1)

Tyke (C1)

ValleyTerrace (C1)

Wally (C1)

Willis (C1)

YoungMoneyMata (C1)

Yucca (C1)

Zalkecks (C1)

Zeenon (C1)

ZygoTaiga (C1)

Myrna (C2)

Phabba (C2)

Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
Plaque NotesWas mostly lytic morphology with plaques about the size of an eraser head. For final dilution, 10^-5 appeared half lytic, half temperate; 10^-4 however appeared purely lytic.
MorphotypeMyoviridae
Has been Phamerated?Yes
Gene List

Click to View

McWolfish_1

McWolfish_2

McWolfish_3
(HNH endonuclease)

McWolfish_4

McWolfish_5

McWolfish_6

McWolfish_7

McWolfish_8

McWolfish_9

McWolfish_10

McWolfish_11

McWolfish_12

McWolfish_13

McWolfish_14

McWolfish_15

McWolfish_16

McWolfish_17

McWolfish_18
(histidine triad domain protein)

McWolfish_19

McWolfish_20

McWolfish_21

McWolfish_22

McWolfish_23

McWolfish_24

McWolfish_25

McWolfish_26

McWolfish_27

McWolfish_28

McWolfish_29

McWolfish_30
(helix-turn-helix DNA binding protein)

McWolfish_31

McWolfish_32

McWolfish_33

McWolfish_34
(ribbon-helix-helix DNA binding domain protein)

McWolfish_35

McWolfish_36

McWolfish_37

McWolfish_38

McWolfish_39

McWolfish_40

McWolfish_41

McWolfish_42

McWolfish_43

McWolfish_44

McWolfish_45

McWolfish_46

McWolfish_47

McWolfish_48

McWolfish_49

McWolfish_50

McWolfish_51

McWolfish_52

McWolfish_53

McWolfish_54
(helix-turn-helix DNA binding protein)

McWolfish_55

McWolfish_56

McWolfish_57

McWolfish_58

McWolfish_59

McWolfish_60

McWolfish_61
(helix-turn-helix DNA binding domain protein)

McWolfish_62
(helix-turn-helix DNA binding domain protein)

McWolfish_63

McWolfish_64

McWolfish_65

McWolfish_66

McWolfish_67

McWolfish_68

McWolfish_69

McWolfish_70

McWolfish_71

McWolfish_72

McWolfish_73

McWolfish_74

McWolfish_75

McWolfish_76

McWolfish_77

McWolfish_78

McWolfish_79

McWolfish_80

McWolfish_81

McWolfish_86
(methyltransferase)

McWolfish_87

McWolfish_88
(portal protein)

McWolfish_89

McWolfish_90

McWolfish_91
(ThyX-like thymidylate synthase)

McWolfish_92

McWolfish_93
(RNA ligase)

McWolfish_94
(LysM-domain protein)

McWolfish_95

McWolfish_96

McWolfish_97
(major capsid protein)

McWolfish_98
(holliday junction resolvase)

McWolfish_99
(capsid decoration protein)

McWolfish_100

McWolfish_101

McWolfish_102

McWolfish_103

McWolfish_104

McWolfish_105

McWolfish_106
(membrane protein)

McWolfish_107

McWolfish_108

McWolfish_109

McWolfish_110

McWolfish_111

McWolfish_112

McWolfish_113

McWolfish_114

McWolfish_115

McWolfish_116

McWolfish_117

McWolfish_118

McWolfish_119
(N-acetyltransferase)

McWolfish_120

McWolfish_121

McWolfish_122

McWolfish_123

McWolfish_124

McWolfish_125
(tail sheath protein)

McWolfish_126

McWolfish_127

McWolfish_128
(tail assembly chaperone)

McWolfish_129
(tail assembly chaperone)

McWolfish_130
(tape measure protein)

McWolfish_131
(minor tail protein)

McWolfish_132

McWolfish_133

McWolfish_134
(minor tail protein)

McWolfish_135

McWolfish_136

McWolfish_137
(baseplate wedge protein)

McWolfish_138
(baseplate wedge protein)

McWolfish_139

McWolfish_140

McWolfish_141

McWolfish_142
(minor tail protein)

McWolfish_143
(minor tail protein)

McWolfish_144

McWolfish_145

McWolfish_146

McWolfish_147

McWolfish_148

McWolfish_149

McWolfish_150

McWolfish_154

McWolfish_155
(peptidyl tRNA hydrolase)

McWolfish_169
(HNH endonuclease)

McWolfish_171
(minor tail protein)

McWolfish_172

McWolfish_184

McWolfish_185
(helicase)

McWolfish_186

McWolfish_187

McWolfish_188

McWolfish_189

McWolfish_190

McWolfish_191

McWolfish_192

McWolfish_193

McWolfish_194

McWolfish_195
(thymidylate kinase)

McWolfish_196
(aminotransferase)

McWolfish_197
(RNA binding domain protein)

McWolfish_198

McWolfish_199
(DnaC-like helicase loader)

McWolfish_200
(DnaB-like dsDNA helicase)

McWolfish_201

McWolfish_202
(DNA primase)

McWolfish_203

McWolfish_204
(DnaJ-like chaperonin)

McWolfish_205
(ssDNA binding domain protein)

McWolfish_206
(DNA polymerase III (alpha))

McWolfish_207
(Rf-1 peptide chain release factor)

McWolfish_208
(RecA-like DNA recombinase)

McWolfish_209
(holliday junction resolvase)

McWolfish_210

McWolfish_211
(HNH endonuclease)

McWolfish_212
(RusA-like resolvase)

McWolfish_213

McWolfish_214

McWolfish_215

McWolfish_216

McWolfish_217
(glycoside hydrolase)

McWolfish_218

McWolfish_219

McWolfish_222

McWolfish_223

McWolfish_224

McWolfish_225

McWolfish_226

McWolfish_227

McWolfish_228

McWolfish_229

McWolfish_230
(Ro-like RNA binding protein)

McWolfish_231

McWolfish_232

McWolfish_233

McWolfish_234

McWolfish_235

McWolfish_236

McWolfish_237
(galactosaminyltransferase)

McWolfish_238

McWolfish_239
(glycosyltransferase)

McWolfish_240
(galactosyltransferase)

McWolfish_241
(nicotinate ribosyltransferase)

McWolfish_242
(glycosyltransferase)

McWolfish_243

McWolfish_244

McWolfish_245

McWolfish_246

McWolfish_247
(lysin A)

McWolfish_248
(membrane protein)

McWolfish_249
(lysin B)

McWolfish_250
(terminase)

McWolfish_251

McWolfish_252

McWolfish_253

McWolfish_254

McWolfish_255
(polynucleotide kinase)

McWolfish_256

McWolfish_257

McWolfish_258
(serine/threonine kinase)

McWolfish_259

McWolfish_260

McWolfish_261
(adenylosuccinate synthetase)

McWolfish_262

McWolfish_263

McWolfish_264

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK112548
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.06x10^14 pfu/mL
Date of SEA Lysate Titering Oct 29, 2014
Pitt Freezer Box# 12
Pitt Freezer Box Grid# B8
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload