Mycobacterium phage Sprinklers
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Detailed Information for Phage Sprinklers
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByKimberly Abels and Shannon Flynn
Year Found2014
Location FoundBoulder, CO USA
Finding InstitutionUniversity of Colorado Boulder
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates40.0076 N, 105.2659 W Map
Discovery NotesMoist, well-lit area, about an hour after the sprinkler ran
8/27/14
7:42 am, 16ºC
Naming NotesSoil sample collected was near a sprinkler
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 9, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage885
Genome length (bp)156060
Character of genome endsCircularly Permuted
GC Content64.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Cluster Life CycleLytic
Other Cluster Members

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Ading (C1)

Adlitam (C1)

Alice (C1)

Amataga (C1)

ArcherS7 (C1)

Asterius (C1)

Astraea (C1)

Atlantean (C1)

Audrick (C1)

Ava3 (C1)

Babyland (C1)

BackyardAgain (C1)

BadAgartude (C1)

BananaFence (C1)

Bangla1971 (C1)

Basquiat (C1)

BeanWater (C1)

BigCity (C1)

Bigswole (C1)

Bipolarisk (C1)

Blackbrain (C1)

Blackdragon (C1)

Blubba (C1)

Bonray (C1)

Bread (C1)

Breeniome (C1)

Burrough (C1)

Bxz1 (C1)

Cactojaque (C1)

Cali (C1)

Cane17 (C1)

Capablanca (C1)

Caravan (C1)

Catera (C1)

Chargie21 (C1)

CharlieB (C1)

ChaylaJr (C1)

ChickenPhender (C1)

Chomp (C1)

CindyLou (C1)

Colt (C1)

Concombre (C1)

Daffodil (C1)

Dandelion (C1)

Darko (C1)

Delilah (C1)

Delylah (C1)

Demodog (C1)

Derek (C1)

Dietrick (C1)

DirtMonster (C1)

Domi (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EasyJones (C1)

EggyFarm (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Flabslab (C1)

Fludd (C1)

FoxtrotP1 (C1)

FrayBell (C1)

FudgeTart (C1)

Gabriel (C1)

Gamgam (C1)

Ghost (C1)

Gizmo (C1)

Grasshills (C1)

Grungle (C1)

Guwapp (C1)

Halldule (C1)

HyRo (C1)

I3 (C1)

IkeLoa (C1)

InigoMontoya (C1)

InterFolia (C1)

Iota (C1)

Issimir (C1)

Izajani (C1)

Janiyra (C1)

Jaygup (C1)

JayJay (C1)

Jedediah (C1)

Jemmno (C1)

Jessibeth14 (C1)

JigglyPuff (C1)

JPickles (C1)

JulietS (C1)

JustHall (C1)

Kamryn (C1)

Kboogie (C1)

Khaleesi (C1)

Koguma (C1)

Latch (C1)

Lee7 (C1)

LifeSavor (C1)

LinStu (C1)

Littleton (C1)

Loanshark (C1)

LolaVinca (C1)

LordLeafolot (C1)

LRRHood (C1)

Lukilu (C1)

Maby2021 (C1)

Mangeria (C1)

Matsumoto (C1)

McGee (C1)

McWolfish (C1)

Megamind (C1)

Melpomini (C1)

MikeLiesIn (C1)

Mikro (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

Naija (C1)

Napoleon13 (C1)

Nappy (C1)

Naval22 (C1)

NedWong (C1)

Ngannou (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

ParkTD (C1)

Patter (C1)

Phlegm (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pinkcreek (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

PurduePete (C1)

QBert (C1)

Quasimodo (C1)

Rabinovish (C1)

Rahel (C1)

Rizal (C1)

RoMag (C1)

Ronan (C1)

Roots515 (C1)

Salacia (C1)

Sauce (C1)

ScottMcG (C1)

Sebata (C1)

Shaqnato (C1)

Shelob (C1)

ShiaLabeouf (C1)

Shifa (C1)

Shnickers (C1)

Shrimp (C1)

SilverDipper (C1)

SmallFry (C1)

Spec (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

SrishMeg2525 (C1)

StephanieG (C1)

Stubby (C1)

StubbySaigey (C1)

Tainan (C1)

Teardrop (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Trinitium (C1)

Turret (C1)

Tyke (C1)

ValleyTerrace (C1)

Wally (C1)

Willis (C1)

Yassified (C1)

YemiJoy2021 (C1)

YoungMoneyMata (C1)

Yucca (C1)

Zalkecks (C1)

Zeenon (C1)

ZygoTaiga (C1)

Greely (C2)

Myrna (C2)

Phabba (C2)

ScoobyDoobyDoo (C2)

Annotating InstitutionUniversity of Colorado Boulder
Annotation StatusIn GenBank
Plaque NotesClear plaques
1-2 mm
Incubated 48 hours at 37ºC
MorphotypeMyoviridae
Number of Genes229
Number of tRNAs35
Number of tmRNAs1
Has been Phamerated?Yes
Gene List

Click to View

Sprinklers_1

Sprinklers_2

Sprinklers_3
(nucleotidyltransferase)

Sprinklers_4

Sprinklers_5

Sprinklers_6

Sprinklers_7

Sprinklers_8

Sprinklers_9

Sprinklers_10

Sprinklers_11

Sprinklers_12

Sprinklers_13

Sprinklers_14

Sprinklers_15

Sprinklers_16

Sprinklers_17

Sprinklers_18

Sprinklers_19

Sprinklers_20

Sprinklers_21

Sprinklers_22

Sprinklers_23

Sprinklers_24

Sprinklers_25

Sprinklers_26

Sprinklers_27

Sprinklers_28

Sprinklers_29

Sprinklers_30

Sprinklers_31

Sprinklers_32

Sprinklers_33

Sprinklers_34

Sprinklers_35
(ribbon-helix-helix DNA binding protein)

Sprinklers_36

Sprinklers_37

Sprinklers_38

Sprinklers_39

Sprinklers_40

Sprinklers_41

Sprinklers_42

Sprinklers_43

Sprinklers_44

Sprinklers_45

Sprinklers_46

Sprinklers_47

Sprinklers_48

Sprinklers_49

Sprinklers_50

Sprinklers_51

Sprinklers_52

Sprinklers_53

Sprinklers_54

Sprinklers_55

Sprinklers_56

Sprinklers_57

Sprinklers_58

Sprinklers_59
(helix-turn-helix DNA binding domain protein)

Sprinklers_60
(helix-turn-helix DNA binding protein)

Sprinklers_61

Sprinklers_62

Sprinklers_63

Sprinklers_64

Sprinklers_65

Sprinklers_66

Sprinklers_67

Sprinklers_68

Sprinklers_69

Sprinklers_70

Sprinklers_71

Sprinklers_72

Sprinklers_73

Sprinklers_74

Sprinklers_75

Sprinklers_76

Sprinklers_77

Sprinklers_78

Sprinklers_79 — tRNA
(tRNA-Ser(gct))

Sprinklers_80 — tRNA
(tRNA-Leu(cag))

Sprinklers_81 — tRNA
(tRNA-Leu(gag))

Sprinklers_82 — tRNA
(tRNA-Leu(caa))

Sprinklers_83

Sprinklers_84

Sprinklers_85
(portal protein)

Sprinklers_86

Sprinklers_87

Sprinklers_88
(ThyX-like thymidylate synthase)

Sprinklers_89

Sprinklers_90
(RNA ligase)

Sprinklers_91
(HNH endonuclease)

Sprinklers_92

Sprinklers_93

Sprinklers_94

Sprinklers_95
(major capsid protein)

Sprinklers_96

Sprinklers_97

Sprinklers_98

Sprinklers_99

Sprinklers_100

Sprinklers_101

Sprinklers_102

Sprinklers_103

Sprinklers_104

Sprinklers_105

Sprinklers_106

Sprinklers_107

Sprinklers_108

Sprinklers_109

Sprinklers_110

Sprinklers_111

Sprinklers_112

Sprinklers_113

Sprinklers_114

Sprinklers_115

Sprinklers_116

Sprinklers_117
(acetyltransferase)

Sprinklers_118

Sprinklers_119

Sprinklers_120

Sprinklers_121

Sprinklers_122

Sprinklers_123
(tail sheath protein)

Sprinklers_124

Sprinklers_125

Sprinklers_126
(tail assembly chaperone)

Sprinklers_127
(tail assembly chaperone)

Sprinklers_128
(tape measure protein)

Sprinklers_129
(minor tail protein)

Sprinklers_130

Sprinklers_131

Sprinklers_132
(minor tail protein)

Sprinklers_133

Sprinklers_134

Sprinklers_135
(baseplate wedge protein)

Sprinklers_136
(baseplate wedge protein)

Sprinklers_137

Sprinklers_138

Sprinklers_139

Sprinklers_140
(minor tail protein)

Sprinklers_141
(minor tail protein)

Sprinklers_142

Sprinklers_143

Sprinklers_144

Sprinklers_145

Sprinklers_146

Sprinklers_147

Sprinklers_148

Sprinklers_149 — tRNA
(tRNA-Pro(tgg))

Sprinklers_150 — tRNA
(tRNA-trp(cca))

Sprinklers_151 — tRNA
(tRNA-trp(gta))

Sprinklers_152

Sprinklers_153
(peptidyl tRNA hydrolase)

Sprinklers_154 — tRNA
(.; tRNA-Arg(tcg))

Sprinklers_155 — tRNA
(tRNA-Leu(cta))

Sprinklers_156 — tRNA
(tRNA-Met(cat))

Sprinklers_157 — tRNA
(tRNA-Cys(gca))

Sprinklers_158 — tRNA
(tRNA-Glu(ctc))

Sprinklers_159 — tRNA
(tRNA-His(gtg))

Sprinklers_160

Sprinklers_161 — tRNA
(tRNA-Ala(tgc))

Sprinklers_162 — tRNA
(tRNA-Phe(gaa))

Sprinklers_163 — tRNA
(tRNA-Val(cac))

Sprinklers_164 — tRNA
(tRNA-Lys(ctt))

Sprinklers_165 — tRNA
(tRNA-Glu(ttc))

Sprinklers_166 — tRNA
(tRNA-Gly(tcc))

Sprinklers_167 — tRNA
(tRNA-Thr(cgt))

Sprinklers_168 — tRNA
(tRNA-Thr(tgt))

Sprinklers_169 — tRNA
(tRNA-Thr(ggt))

Sprinklers_170
(minor tail protein)

Sprinklers_171

Sprinklers_172 — tRNA
(tRNA-Gly(gcc))

Sprinklers_173 — tRNA
(tRNA-Asp(gtc))

Sprinklers_174 — tRNA
(tRNA-Met(cat))

Sprinklers_175 — tRNA
(tRNA-Ile(gat))

Sprinklers_176 — tRNA
(tRNA-Arg(acg))

Sprinklers_177 — tRNA
(tRNA-Val(gac))

Sprinklers_178 — tRNA
(tRNA-GLy(ttc))

Sprinklers_179 — tRNA
(tRNA-Arg(cct))

Sprinklers_180 — tmRNA
(tmRNA)

Sprinklers_181 — tRNA
(tRNA-Gln(ttg))

Sprinklers_182 — tRNA
(tRNA-Arg(tct))

Sprinklers_183 — tRNA
(tRNA- Lys(ttt))

Sprinklers_184

Sprinklers_185
(DNA helicase)

Sprinklers_186

Sprinklers_187

Sprinklers_188

Sprinklers_189

Sprinklers_190

Sprinklers_191

Sprinklers_192

Sprinklers_193

Sprinklers_194

Sprinklers_195
(thymidylate kinase)

Sprinklers_196
(aminotransferase)

Sprinklers_197

Sprinklers_198

Sprinklers_199

Sprinklers_200
(DnaC-like helicase loader)

Sprinklers_201
(DnaB-like dsDNA helicase)

Sprinklers_202

Sprinklers_203
(DNA primase)

Sprinklers_204

Sprinklers_205
(DnaJ-like chaperonin)

Sprinklers_206
(ssDNA binding protein)

Sprinklers_207
(DnaE-like DNA polymerase III)

Sprinklers_208
(RF-1 peptide chain release factor)

Sprinklers_209
(RecA-like DNA recombinase)

Sprinklers_210
(holliday junction resolvase)

Sprinklers_211

Sprinklers_212
(HNH endonuclease)

Sprinklers_213
(RusA-like resolvase)

Sprinklers_214

Sprinklers_215

Sprinklers_216

Sprinklers_217

Sprinklers_218

Sprinklers_219

Sprinklers_220

Sprinklers_221 — tRNA
(tRNA-Gln(ctg))

Sprinklers_222 — tRNA
(tRNA-Asn(gtt))

Sprinklers_223

Sprinklers_224

Sprinklers_225

Sprinklers_226

Sprinklers_227

Sprinklers_228

Sprinklers_229

Sprinklers_230

Sprinklers_231
(Ro-like RNA binding protein)

Sprinklers_232

Sprinklers_233

Sprinklers_234

Sprinklers_235

Sprinklers_236

Sprinklers_237

Sprinklers_238
(glycosyltransferase)

Sprinklers_239

Sprinklers_240
(glycosyltransferase)

Sprinklers_241
(glycosyltransferase)

Sprinklers_242
(PnuC-like nicotinamide riboside transporter)

Sprinklers_243
(glycosyltransferase)

Sprinklers_244

Sprinklers_245

Sprinklers_246

Sprinklers_247

Sprinklers_248
(lysin A)

Sprinklers_249

Sprinklers_250
(lysin B)

Sprinklers_251
(terminase)

Sprinklers_252

Sprinklers_253

Sprinklers_254

Sprinklers_255

Sprinklers_256
(polynucleotide kinase)

Sprinklers_257

Sprinklers_258

Sprinklers_259
(serine/threonine kinase)

Sprinklers_260

Sprinklers_261

Sprinklers_262
(PurA-like adenylosuccinate synthetase)

Sprinklers_263

Sprinklers_264

Sprinklers_265

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK359355
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.9x10e10
Date of SEA Lysate Titering Sep 26, 2014
Pitt Freezer Box# 9
Pitt Freezer Box Grid# B12
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload