Mycobacterium phage Ngannou
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Detailed Information for Phage Ngannou
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByRohit Anandani
Year Found2021
Location FoundBethlehem, PA United States of America
Finding InstitutionLehigh University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature5°C
GPS Coordinates40.70065 N, 75.32206 W Map
Discovery NotesThe soil was from the backyard of my home in Bethlehem. The soil was dry and moist. It was also dark in color. It was extracted three inches below the surface.
Naming NotesThe name Ngannou comes from the athlete Francis Ngannou. I really admire Francis and I think my phages are tough and durable similarly.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 13, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage361
Genome length (bp)154238
Character of genome endsCircularly Permuted
GC Content64.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Cluster Life CycleLytic
Other Cluster Members

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Ading (C1)

Adlitam (C1)

Alice (C1)

Amataga (C1)

ArcherS7 (C1)

Asterius (C1)

Astraea (C1)

Atlantean (C1)

Audrick (C1)

Ava3 (C1)

Babyland (C1)

BackyardAgain (C1)

BadAgartude (C1)

BananaFence (C1)

Bangla1971 (C1)

Basquiat (C1)

BeanWater (C1)

BigCity (C1)

Bigswole (C1)

Bipolarisk (C1)

Blackbrain (C1)

Blubba (C1)

Bonray (C1)

Bread (C1)

Breeniome (C1)

Burrough (C1)

Bxz1 (C1)

Cactojaque (C1)

Cali (C1)

Cane17 (C1)

Capablanca (C1)

Caravan (C1)

Catera (C1)

Chargie21 (C1)

CharlieB (C1)

ChaylaJr (C1)

ChickenPhender (C1)

Chomp (C1)

CindyLou (C1)

Colt (C1)

Concombre (C1)

Daffodil (C1)

Dandelion (C1)

Darko (C1)

Delilah (C1)

Delylah (C1)

Derek (C1)

Dietrick (C1)

DirtMonster (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EasyJones (C1)

EggyFarm (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Flabslab (C1)

Fludd (C1)

FoxtrotP1 (C1)

FrayBell (C1)

FudgeTart (C1)

Gabriel (C1)

Gamgam (C1)

Ghost (C1)

Gizmo (C1)

Grasshills (C1)

Grungle (C1)

Guwapp (C1)

Halldule (C1)

HyRo (C1)

I3 (C1)

IkeLoa (C1)

InigoMontoya (C1)

InterFolia (C1)

Iota (C1)

Issimir (C1)

Izajani (C1)

Janiyra (C1)

Jaygup (C1)

JayJay (C1)

Jedediah (C1)

Jemmno (C1)

Jessibeth14 (C1)

JPickles (C1)

JulietS (C1)

JustHall (C1)

Kamryn (C1)

Kboogie (C1)

Khaleesi (C1)

Koguma (C1)

Latch (C1)

Lee7 (C1)

LifeSavor (C1)

LinStu (C1)

Littleton (C1)

Loanshark (C1)

LolaVinca (C1)

LordLeafolot (C1)

LRRHood (C1)

Lukilu (C1)

Maby2021 (C1)

Mangeria (C1)

Matsumoto (C1)

McGee (C1)

McWolfish (C1)

Megamind (C1)

Melpomini (C1)

MikeLiesIn (C1)

Mikro (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

Naija (C1)

Napoleon13 (C1)

Nappy (C1)

Naval22 (C1)

NedWong (C1)

Ngannou (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

ParkTD (C1)

Patter (C1)

Phlegm (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pinkcreek (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

PurduePete (C1)

QBert (C1)

Quasimodo (C1)

Rabinovish (C1)

Rahel (C1)

Rizal (C1)

RoMag (C1)

Ronan (C1)

Roots515 (C1)

Salacia (C1)

Sauce (C1)

ScottMcG (C1)

Sebata (C1)

Shaqnato (C1)

Shelob (C1)

ShiaLabeouf (C1)

Shifa (C1)

Shnickers (C1)

Shrimp (C1)

SilverDipper (C1)

SmallFry (C1)

Spec (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

SrishMeg2525 (C1)

StephanieG (C1)

Stubby (C1)

StubbySaigey (C1)

Teardrop (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Trinitium (C1)

Turret (C1)

Tyke (C1)

ValleyTerrace (C1)

Wally (C1)

Willis (C1)

Yassified (C1)

YemiJoy2021 (C1)

YoungMoneyMata (C1)

Yucca (C1)

Zalkecks (C1)

Zeenon (C1)

ZygoTaiga (C1)

Greely (C2)

Myrna (C2)

Phabba (C2)

ScoobyDoobyDoo (C2)

Annotating InstitutionLehigh University
Annotation StatusIn GenBank
Plaque NotesMy plaques all had the same morphology. They were all about 1mm in diameter, clear, and had a circular border.
MorphotypeMyoviridae
Has been Phamerated?Yes
Gene List

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Ngannou_1

Ngannou_2

Ngannou_3
(nucleotidyltransferase)

Ngannou_4

Ngannou_5

Ngannou_6

Ngannou_7

Ngannou_8

Ngannou_9

Ngannou_10

Ngannou_11

Ngannou_12

Ngannou_13

Ngannou_14

Ngannou_15

Ngannou_16

Ngannou_17
(histidine triad nucleotide binding protein)

Ngannou_18

Ngannou_19

Ngannou_20

Ngannou_21

Ngannou_22

Ngannou_23

Ngannou_24

Ngannou_25

Ngannou_26

Ngannou_27
(membrane protein, Band-7-like)

Ngannou_28

Ngannou_29
(protease)

Ngannou_30

Ngannou_31

Ngannou_32

Ngannou_33
(ribbon-helix-helix DNA binding domain)

Ngannou_34

Ngannou_35

Ngannou_36

Ngannou_37

Ngannou_38

Ngannou_39

Ngannou_40

Ngannou_41

Ngannou_42

Ngannou_43

Ngannou_44

Ngannou_45

Ngannou_46

Ngannou_47

Ngannou_48

Ngannou_49

Ngannou_50
(helix-turn-helix DNA binding domain)

Ngannou_51
(HNH endonuclease)

Ngannou_52

Ngannou_53

Ngannou_54

Ngannou_55

Ngannou_56

Ngannou_57
(helix-turn-helix DNA Binding domain)

Ngannou_58
(helix-turn-helix DNA Binding domain)

Ngannou_59

Ngannou_60

Ngannou_61

Ngannou_62

Ngannou_63

Ngannou_64

Ngannou_65

Ngannou_66

Ngannou_67

Ngannou_68

Ngannou_69

Ngannou_70

Ngannou_71

Ngannou_72

Ngannou_73

Ngannou_74

Ngannou_75

Ngannou_76

Ngannou_77

Ngannou_78

Ngannou_79
(ParB-like nuclease domain)

Ngannou_84

Ngannou_85

Ngannou_86
(portal protein)

Ngannou_87

Ngannou_88

Ngannou_89
(ThyX-like thymidylate synthase)

Ngannou_90

Ngannou_91
(RNA Ligase)

Ngannou_92
(HNH endonuclease)

Ngannou_93

Ngannou_94

Ngannou_95

Ngannou_96
(major capsid protein)

Ngannou_97

Ngannou_98
(head fiber protein)

Ngannou_99

Ngannou_100

Ngannou_101

Ngannou_102

Ngannou_103

Ngannou_104

Ngannou_105

Ngannou_106

Ngannou_107

Ngannou_108

Ngannou_109

Ngannou_110

Ngannou_111

Ngannou_112

Ngannou_113

Ngannou_114

Ngannou_115

Ngannou_116

Ngannou_117

Ngannou_118

Ngannou_119

Ngannou_120

Ngannou_121

Ngannou_122

Ngannou_123

Ngannou_124
(tail sheath protein)

Ngannou_125

Ngannou_126

Ngannou_127
(tail assembly chaperone)

Ngannou_128
(tail assembly chaperone)

Ngannou_129
(tape measure protein)

Ngannou_130

Ngannou_131

Ngannou_132

Ngannou_133
(minor tail protein)

Ngannou_134
(minor tail protein)

Ngannou_135
(minor tail protein)

Ngannou_136
(baseplate wedge protein)

Ngannou_137
(baseplate wedge protein)

Ngannou_138

Ngannou_139

Ngannou_140

Ngannou_141

Ngannou_142
(minor tail protein)

Ngannou_143

Ngannou_144

Ngannou_145

Ngannou_146

Ngannou_147

Ngannou_148

Ngannou_149

Ngannou_153

Ngannou_154
(peptidyl tRNA hydrolase)

Ngannou_161

Ngannou_169
(HNH endonuclease)

Ngannou_171
(minor tail protein)

Ngannou_172

Ngannou_183

Ngannou_184
(DNA helicase)

Ngannou_185

Ngannou_186

Ngannou_187

Ngannou_188

Ngannou_189

Ngannou_190

Ngannou_191

Ngannou_192

Ngannou_193

Ngannou_194
(thymidylate kinase)

Ngannou_195
(aminotransferase)

Ngannou_196

Ngannou_197

Ngannou_198

Ngannou_199
(DnaC-like helicase loader)

Ngannou_200
(DnaB-like dsDNA helicase)

Ngannou_201

Ngannou_202
(DNA primase)

Ngannou_203

Ngannou_204
(chaperonin, DnaJ-like)

Ngannou_205
(SSB protein)

Ngannou_206
(DnaE-like DNA polymerase III)

Ngannou_207
(RF-1 peptide chain release factor)

Ngannou_208
(RecA-like DNA recombinase)

Ngannou_209
(Holliday junction resolvase)

Ngannou_210

Ngannou_211
(HNH endonuclease)

Ngannou_212
(RusA-like resolvase)

Ngannou_213

Ngannou_214

Ngannou_215

Ngannou_216

Ngannou_217

Ngannou_218

Ngannou_219
(HNH endonuclease)

Ngannou_222

Ngannou_223

Ngannou_224

Ngannou_225

Ngannou_226

Ngannou_227

Ngannou_228
(Ro-like RNA binding protein)

Ngannou_229

Ngannou_230

Ngannou_231

Ngannou_232

Ngannou_233

Ngannou_234

Ngannou_235
(glycosyltransferase)

Ngannou_236

Ngannou_237
(glycosyltransferase)

Ngannou_238
(glycosyltransferase)

Ngannou_239
(PnuC-like Nicotinamide riboside transporter)

Ngannou_240
(glycosyltransferase)

Ngannou_241

Ngannou_242

Ngannou_243

Ngannou_244

Ngannou_245
(lysin A)

Ngannou_246
(holin)

Ngannou_247
(lysin B)

Ngannou_248
(terminase)

Ngannou_249

Ngannou_250

Ngannou_251

Ngannou_252

Ngannou_253
(polynucleotide kinase)

Ngannou_254

Ngannou_255
(serine/threonine kinase)

Ngannou_256

Ngannou_257

Ngannou_258
(adenylosuccinate synthetase, PurA-like)

Ngannou_259

Ngannou_260

Ngannou_261

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionPP405137
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 143
Pitt Freezer Box Grid# A5
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload