| Detailed Information for Phage Ngannou |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Rohit Anandani |
| Year Found | 2021 |
| Location Found | Bethlehem, PA United States of America |
| Finding Institution | Lehigh University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 5°C |
| GPS Coordinates | 40.70065 N, 75.32206 W Map |
| Discovery Notes | The soil was from the backyard of my home in Bethlehem. The soil was dry and moist. It was also dark in color. It was extracted three inches below the surface. |
| Naming Notes | The name Ngannou comes from the athlete Francis Ngannou. I really admire Francis and I think my phages are tough and durable similarly. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 13, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 361 |
| Genome length (bp) | 154238 |
| Character of genome ends | Circularly Permuted |
| GC Content | 64.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | C |
| Subcluster | C1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Lehigh University |
| Annotation Status | In GenBank |
| Plaque Notes | My plaques all had the same morphology. They were all about 1mm in diameter, clear, and had a circular border. |
| Morphotype | Myoviridae |
| Number of Genes | 227 |
| Number of tRNAs | 32 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PP405137 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
143 |
| Pitt Freezer Box Grid# |
A5 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
