Mycobacterium phage Alice
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Detailed Information for Phage Alice
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByCoreen M. Manley
Year Found2009
Location FoundAledo, TX USA
Finding InstitutionUniversity of North Texas
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates32.688207 N, 97.648082 W Map
Discovery NotesFound in a compost pile in a garden. The pile had been there for about 6 months so lots of decaying matter, mainly from fruit and vegetables. The soil was damp and it was moderately warm compared with the air temperature.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJul 29, 2010
Genome length (bp)153401
Character of genome endsCircularly Permuted
GC Content64.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Cluster Life CycleLytic
Other Cluster Members

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Ading (C1)

Adlitam (C1)

Alice (C1)

Amataga (C1)

ArcherS7 (C1)

Asterius (C1)

Astraea (C1)

Atlantean (C1)

Audrick (C1)

Ava3 (C1)

Babyland (C1)

BackyardAgain (C1)

BadAgartude (C1)

BananaFence (C1)

Bangla1971 (C1)

Basquiat (C1)

BeanWater (C1)

BigCity (C1)

Bigswole (C1)

Bipolarisk (C1)

Blackbrain (C1)

Blackdragon (C1)

Blubba (C1)

Bonray (C1)

Bread (C1)

Breeniome (C1)

Burrough (C1)

Bxz1 (C1)

Cactojaque (C1)

Cali (C1)

Cane17 (C1)

Capablanca (C1)

Caravan (C1)

Catera (C1)

Chargie21 (C1)

CharlieB (C1)

ChaylaJr (C1)

ChickenPhender (C1)

Chomp (C1)

CindyLou (C1)

Colt (C1)

Concombre (C1)

Daffodil (C1)

Dandelion (C1)

Darko (C1)

Delilah (C1)

Delylah (C1)

Demodog (C1)

Derek (C1)

Dietrick (C1)

DirtMonster (C1)

Domi (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EasyJones (C1)

EggyFarm (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Flabslab (C1)

Fludd (C1)

FoxtrotP1 (C1)

FrayBell (C1)

FudgeTart (C1)

Gabriel (C1)

Gamgam (C1)

Ghost (C1)

Gizmo (C1)

Grasshills (C1)

Grungle (C1)

Guwapp (C1)

Halldule (C1)

HyRo (C1)

I3 (C1)

IkeLoa (C1)

InigoMontoya (C1)

InterFolia (C1)

Iota (C1)

Issimir (C1)

Izajani (C1)

Janiyra (C1)

Jaygup (C1)

JayJay (C1)

Jedediah (C1)

Jemmno (C1)

Jessibeth14 (C1)

JigglyPuff (C1)

JPickles (C1)

JulietS (C1)

JustHall (C1)

Kamryn (C1)

Kboogie (C1)

Khaleesi (C1)

Koguma (C1)

Latch (C1)

Lee7 (C1)

LifeSavor (C1)

LinStu (C1)

Littleton (C1)

Loanshark (C1)

LolaVinca (C1)

LordLeafolot (C1)

LRRHood (C1)

Lukilu (C1)

Maby2021 (C1)

Mangeria (C1)

Matsumoto (C1)

McGee (C1)

McWolfish (C1)

Megamind (C1)

Melpomini (C1)

MikeLiesIn (C1)

Mikro (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

Naija (C1)

Napoleon13 (C1)

Nappy (C1)

Naval22 (C1)

NedWong (C1)

Ngannou (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

ParkTD (C1)

Patter (C1)

Phlegm (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pinkcreek (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

PurduePete (C1)

QBert (C1)

Quasimodo (C1)

Rabinovish (C1)

Rahel (C1)

Rizal (C1)

RoMag (C1)

Ronan (C1)

Roots515 (C1)

Salacia (C1)

Sauce (C1)

ScottMcG (C1)

Sebata (C1)

Shaqnato (C1)

Shelob (C1)

ShiaLabeouf (C1)

Shifa (C1)

Shnickers (C1)

Shrimp (C1)

SilverDipper (C1)

SmallFry (C1)

Spec (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

SrishMeg2525 (C1)

StephanieG (C1)

Stubby (C1)

StubbySaigey (C1)

Tainan (C1)

Teardrop (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Trinitium (C1)

Turret (C1)

Tyke (C1)

ValleyTerrace (C1)

Wally (C1)

Willis (C1)

Yassified (C1)

YemiJoy2021 (C1)

YoungMoneyMata (C1)

Yucca (C1)

Zalkecks (C1)

Zeenon (C1)

ZygoTaiga (C1)

Greely (C2)

Myrna (C2)

Phabba (C2)

ScoobyDoobyDoo (C2)

Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque Notes1mm and 1.5mm plaques, very clear, 2 sizes
24--36 hour incubation period in 37 degrees Celsius conditions
MorphotypeMyoviridae
Number of Genes221
Number of tRNAs29
Number of tmRNAs1
Has been Phamerated?Yes
Gene List

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Alice_1

Alice_2

Alice_3
(nucleotidyltransferase)

Alice_4

Alice_5

Alice_6

Alice_7

Alice_8

Alice_9

Alice_10

Alice_11

Alice_12

Alice_13

Alice_14

Alice_15

Alice_16

Alice_17

Alice_18

Alice_19

Alice_20

Alice_21

Alice_22

Alice_23

Alice_24
(band-7-like membrane protein)

Alice_25

Alice_26

Alice_27

Alice_28

Alice_29

Alice_30

Alice_31

Alice_32

Alice_33

Alice_34

Alice_35

Alice_36

Alice_37

Alice_38

Alice_39

Alice_40

Alice_41

Alice_42

Alice_43

Alice_44

Alice_45

Alice_46

Alice_47

Alice_48

Alice_49

Alice_50

Alice_51

Alice_52

Alice_53

Alice_54

Alice_55

Alice_56

Alice_57
(helix-turn-helix DNA binding domain protein)

Alice_58

Alice_59

Alice_60

Alice_61

Alice_62

Alice_63

Alice_64

Alice_65

Alice_66

Alice_67

Alice_68

Alice_69

Alice_70

Alice_71

Alice_72

Alice_73

Alice_74

Alice_75

Alice_81

Alice_82

Alice_83

Alice_84

Alice_85

Alice_86
(ThyX-like thymidylate synthase)

Alice_87

Alice_88
(RNA ligase)

Alice_89

Alice_90

Alice_91

Alice_92

Alice_93

Alice_94

Alice_95

Alice_96

Alice_97

Alice_98

Alice_99

Alice_100

Alice_101

Alice_102

Alice_103

Alice_104

Alice_105

Alice_106

Alice_107

Alice_108

Alice_109

Alice_110

Alice_111

Alice_112

Alice_113

Alice_114

Alice_115

Alice_116

Alice_117

Alice_118

Alice_119

Alice_120
(tail sheath protein)

Alice_121

Alice_122

Alice_123
(tail assembly chaperone)

Alice_124
(tail assembly chaperone)

Alice_125
(tape measure protein)

Alice_126

Alice_127

Alice_128

Alice_129
(minor tail protein)

Alice_130

Alice_131
(minor tail protein)

Alice_132

Alice_133
(baseplate J protein)

Alice_134

Alice_135

Alice_136

Alice_137

Alice_138
(minor tail protein)

Alice_139

Alice_140

Alice_141

Alice_142

Alice_143

Alice_144

Alice_148

Alice_149

Alice_150
(peptidyl tRNA hydrolase)

Alice_159
(minor tail protein)

Alice_160

Alice_173

Alice_174

Alice_175
(DNA helicase)

Alice_176

Alice_177

Alice_178

Alice_179

Alice_180

Alice_181

Alice_182

Alice_183

Alice_184

Alice_185
(aminotransferase)

Alice_186

Alice_187

Alice_188

Alice_189
(DnaC-like helicase loader)

Alice_190
(DnaB-like dsDNA helicase)

Alice_191

Alice_192
(DNA primase)

Alice_193

Alice_194
(DnaJ-like chaperonin)

Alice_195

Alice_196
(DnaE-like DNA polymerase III)

Alice_197
(RF-1 peptide chain release factor)

Alice_198
(RecA-like DNA recombinase)

Alice_199

Alice_200

Alice_201

Alice_202
(RusA-like resolvase)

Alice_203

Alice_204

Alice_205

Alice_206

Alice_207

Alice_208

Alice_209

Alice_212

Alice_213

Alice_214

Alice_215

Alice_216

Alice_217

Alice_218

Alice_219

Alice_220
(Ro-like RNA binding protein)

Alice_221

Alice_222

Alice_223

Alice_224

Alice_225

Alice_226
(glycosyltransferase)

Alice_227

Alice_228

Alice_229
(glycosyltransferase)

Alice_230

Alice_231
(glycosyltransferase)

Alice_232

Alice_233

Alice_234

Alice_235

Alice_236
(lysin A)

Alice_237
(holin)

Alice_238
(lysin B)

Alice_239
(terminase)

Alice_240

Alice_241

Alice_242

Alice_243

Alice_244
(polynucleotide kinase)

Alice_245

Alice_246
(serine/threonine kinase)

Alice_247

Alice_248

Alice_249
(PurA-like adenylosuccinate synthetase)

Alice_250

Alice_251

Alice_252

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionJF704092
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
SEA Lysate Titer 11.9e10
Pitt Freezer Box# 9
Pitt Freezer Box Grid# 45
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
Virtual Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload