Detailed Information for Phage Alice |
Discovery Information |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Coreen M. Manley |
Year Found | 2009 |
Location Found | Aledo, TX USA |
Finding Institution | University of North Texas |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | No |
Isolation Temperature | Not entered |
GPS Coordinates | 32.688207 N, 97.648082 W Map |
Discovery Notes | Found in a compost pile in a garden. The pile had been there for about 6 months so lots of decaying matter, mainly from fruit and vegetables. The soil was damp and it was moderately warm compared with the air temperature. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Jul 29, 2010 |
Genome length (bp) | 153401 |
Character of genome ends | Circularly Permuted |
GC Content | 64.7% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | C |
Subcluster | C1 |
Cluster Life Cycle | Lytic |
Other Cluster Members |
|
Annotating Institution | Unknown or unassigned |
Annotation Status | In GenBank |
Plaque Notes | 1mm and 1.5mm plaques, very clear, 2 sizes 24--36 hour incubation period in 37 degrees Celsius conditions
|
Morphotype | Myoviridae |
Number of Genes | 221 |
Number of tRNAs | 29 |
Number of tmRNAs | 1 |
Has been Phamerated? | Yes |
Gene List |
|
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | JF704092 |
Refseq Number | None yet |
Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
Archiving Info |
Archiving status |
Archived |
SEA Lysate Titer |
11.9e10 |
Pitt Freezer Box# |
9 |
Pitt Freezer Box Grid# |
45 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
Virtual Digest Picture | Download |
EM Picture | Download |
Final DNAMaster File | Download |