Mycobacterium phage BackyardAgain
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Detailed Information for Phage BackyardAgain
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByAllen Pruitt and Ashlynn Boone
Year Found2019
Location FoundSalem, VA United States
Finding InstitutionVirginia Western Community College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates37.380169 N, 80.079468 W Map
Discovery NotesSample was found in backyard of Allen Pruitt's home. Soil retrieved from a patch of dead grass of somewhat moist condition. Soil was retrieved from a depth of around two or three inches below the surface.
Naming NotesPhage was named, "BackyardAgain" due to location of origin. Sample was retrieved from the backyard of the Pruitt family and was the second sample retrieved from a close proximity of that area. The name is also a play on words based on the children's show, "Backyardigans".
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 9, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage635
Genome length (bp)153731
Character of genome endsCircularly Permuted
GC Content64.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Cluster Life CycleLytic
Other Cluster Members

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Ading (C1)

Adlitam (C1)

Alice (C1)

Amataga (C1)

ArcherS7 (C1)

Asterius (C1)

Astraea (C1)

Atlantean (C1)

Audrick (C1)

Ava3 (C1)

Babyland (C1)

BackyardAgain (C1)

BadAgartude (C1)

BananaFence (C1)

Bangla1971 (C1)

Basquiat (C1)

BeanWater (C1)

BigCity (C1)

Bigswole (C1)

Bipolarisk (C1)

Blackbrain (C1)

Blackdragon (C1)

Blubba (C1)

Bonray (C1)

Bread (C1)

Breeniome (C1)

Burrough (C1)

Bxz1 (C1)

Cactojaque (C1)

Cali (C1)

Cane17 (C1)

Capablanca (C1)

Caravan (C1)

Catera (C1)

Chargie21 (C1)

CharlieB (C1)

ChaylaJr (C1)

ChickenPhender (C1)

Chomp (C1)

CindyLou (C1)

Colt (C1)

Concombre (C1)

Daffodil (C1)

Dandelion (C1)

Darko (C1)

Delilah (C1)

Delylah (C1)

Demodog (C1)

Derek (C1)

Dietrick (C1)

DirtMonster (C1)

Domi (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EasyJones (C1)

EggyFarm (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Flabslab (C1)

Fludd (C1)

FoxtrotP1 (C1)

FrayBell (C1)

FudgeTart (C1)

Gabriel (C1)

Gamgam (C1)

Ghost (C1)

Gizmo (C1)

Grasshills (C1)

Grungle (C1)

Guwapp (C1)

Halldule (C1)

HyRo (C1)

I3 (C1)

IkeLoa (C1)

InigoMontoya (C1)

InterFolia (C1)

Iota (C1)

Issimir (C1)

Izajani (C1)

Janiyra (C1)

Jaygup (C1)

JayJay (C1)

Jedediah (C1)

Jemmno (C1)

Jessibeth14 (C1)

JigglyPuff (C1)

JPickles (C1)

JulietS (C1)

JustHall (C1)

Kamryn (C1)

Kboogie (C1)

Khaleesi (C1)

Koguma (C1)

Latch (C1)

Lee7 (C1)

LifeSavor (C1)

LinStu (C1)

Littleton (C1)

Loanshark (C1)

LolaVinca (C1)

LordLeafolot (C1)

LRRHood (C1)

Lukilu (C1)

Maby2021 (C1)

Mangeria (C1)

Matsumoto (C1)

McGee (C1)

McWolfish (C1)

Megamind (C1)

Melpomini (C1)

MikeLiesIn (C1)

Mikro (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

Naija (C1)

Napoleon13 (C1)

Nappy (C1)

Naval22 (C1)

NedWong (C1)

Ngannou (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

ParkTD (C1)

Patter (C1)

Phlegm (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pinkcreek (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

PurduePete (C1)

QBert (C1)

Quasimodo (C1)

Rabinovish (C1)

Rahel (C1)

Rizal (C1)

RoMag (C1)

Ronan (C1)

Roots515 (C1)

Salacia (C1)

Sauce (C1)

ScottMcG (C1)

Sebata (C1)

Shaqnato (C1)

Shelob (C1)

ShiaLabeouf (C1)

Shifa (C1)

Shnickers (C1)

Shrimp (C1)

SilverDipper (C1)

SmallFry (C1)

Spec (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

SrishMeg2525 (C1)

StephanieG (C1)

Stubby (C1)

StubbySaigey (C1)

Tainan (C1)

Teardrop (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Trinitium (C1)

Turret (C1)

Tyke (C1)

ValleyTerrace (C1)

Wally (C1)

Willis (C1)

Yassified (C1)

YemiJoy2021 (C1)

YoungMoneyMata (C1)

Yucca (C1)

Zalkecks (C1)

Zeenon (C1)

ZygoTaiga (C1)

Greely (C2)

Myrna (C2)

Phabba (C2)

ScoobyDoobyDoo (C2)

Annotating InstitutionVirginia Western Community College
Annotation StatusIn GenBank
MorphotypeMyoviridae
Number of Genes227
Number of tRNAs33
Number of tmRNAs1
Has been Phamerated?Yes
Gene List

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BackyardAgain_1

BackyardAgain_2

BackyardAgain_3
(nucleotidyl transferase)

BackyardAgain_4

BackyardAgain_5

BackyardAgain_6

BackyardAgain_7

BackyardAgain_8

BackyardAgain_9

BackyardAgain_10

BackyardAgain_11

BackyardAgain_12

BackyardAgain_13

BackyardAgain_14

BackyardAgain_15

BackyardAgain_16
(histidine triad nucleotide binding protein)

BackyardAgain_17

BackyardAgain_18

BackyardAgain_19

BackyardAgain_20

BackyardAgain_21

BackyardAgain_22

BackyardAgain_23

BackyardAgain_24

BackyardAgain_25

BackyardAgain_26
(band-7-like membrane protein)

BackyardAgain_27

BackyardAgain_28
(helix-turn-helix DNA binding domain protein)

BackyardAgain_29

BackyardAgain_30

BackyardAgain_31

BackyardAgain_32
(ribbon-helix-helix DNA binding domain protein)

BackyardAgain_33

BackyardAgain_34

BackyardAgain_35

BackyardAgain_36

BackyardAgain_37

BackyardAgain_38

BackyardAgain_39

BackyardAgain_40

BackyardAgain_41

BackyardAgain_42

BackyardAgain_43

BackyardAgain_44

BackyardAgain_45

BackyardAgain_46

BackyardAgain_47

BackyardAgain_48

BackyardAgain_49

BackyardAgain_50

BackyardAgain_51

BackyardAgain_52

BackyardAgain_53
(helix-turn-helix DNA binding domain protein)

BackyardAgain_54

BackyardAgain_55

BackyardAgain_56

BackyardAgain_57

BackyardAgain_58

BackyardAgain_59

BackyardAgain_60
(helix-turn-helix DNA binding domain protein)

BackyardAgain_61
(helix-turn-helix DNA binding domain protein)

BackyardAgain_62

BackyardAgain_63

BackyardAgain_64

BackyardAgain_65

BackyardAgain_66

BackyardAgain_67

BackyardAgain_68

BackyardAgain_69

BackyardAgain_70

BackyardAgain_71

BackyardAgain_72

BackyardAgain_73

BackyardAgain_74

BackyardAgain_75

BackyardAgain_76

BackyardAgain_77

BackyardAgain_78

BackyardAgain_79

BackyardAgain_80

BackyardAgain_81 — tRNA
(tRNA-Ser(gct))

BackyardAgain_82 — tRNA
(tRNA-Leu(cag))

BackyardAgain_83 — tRNA
(tRNA-Leu(gag))

BackyardAgain_84 — tRNA
(tRNA-Leu(caa))

BackyardAgain_85

BackyardAgain_86

BackyardAgain_87
(portal protein)

BackyardAgain_88

BackyardAgain_89

BackyardAgain_90
(ThyX-like thymidylate synthase)

BackyardAgain_91

BackyardAgain_92
(RNA ligase)

BackyardAgain_93

BackyardAgain_94

BackyardAgain_95

BackyardAgain_96
(major capsid protein)

BackyardAgain_97
(holliday junction resolvase)

BackyardAgain_98

BackyardAgain_99

BackyardAgain_100

BackyardAgain_101

BackyardAgain_102

BackyardAgain_103

BackyardAgain_104

BackyardAgain_105

BackyardAgain_106

BackyardAgain_107

BackyardAgain_108

BackyardAgain_109

BackyardAgain_110

BackyardAgain_111

BackyardAgain_112

BackyardAgain_113

BackyardAgain_114

BackyardAgain_115

BackyardAgain_116

BackyardAgain_117

BackyardAgain_118

BackyardAgain_119

BackyardAgain_120

BackyardAgain_121
(ParB-like nuclease domain protein)

BackyardAgain_122

BackyardAgain_123

BackyardAgain_124
(tail sheath protein)

BackyardAgain_125

BackyardAgain_126

BackyardAgain_127
(tail assembly chaperone)

BackyardAgain_128
(tail assembly chaperone)

BackyardAgain_129
(tape measure protein)

BackyardAgain_130
(minor tail protein)

BackyardAgain_131

BackyardAgain_132

BackyardAgain_133
(hydrolase)

BackyardAgain_134

BackyardAgain_135

BackyardAgain_136
(baseplate wedge protein)

BackyardAgain_137
(baseplate wedge protein)

BackyardAgain_138

BackyardAgain_139

BackyardAgain_140

BackyardAgain_141
(minor tail protein)

BackyardAgain_142
(minor tail protein)

BackyardAgain_143

BackyardAgain_144

BackyardAgain_145

BackyardAgain_146

BackyardAgain_147

BackyardAgain_148

BackyardAgain_149

BackyardAgain_150 — tRNA
(tRNA-Pro(tgg))

BackyardAgain_151 — tRNA
(tRNA-Trp(cca))

BackyardAgain_152 — tRNA
(tRNA-Tyr(gta))

BackyardAgain_153

BackyardAgain_154
(peptidyl tRNA hydrolase)

BackyardAgain_155 — tRNA
(tRNA-Pyl(cta))

BackyardAgain_156 — tRNA
(tRNA-Met(cat))

BackyardAgain_157 — tRNA
(tRNA-Cys(gca))

BackyardAgain_158 — tRNA
(tRNA-Glu(ctc))

BackyardAgain_159 — tRNA
(tRNA-His(gtg))

BackyardAgain_160 — tRNA
(tRNA-Ala(tgc))

BackyardAgain_161

BackyardAgain_162 — tRNA
(tRNA-Phe(gaa))

BackyardAgain_163 — tRNA
(tRNA-Val(cac))

BackyardAgain_164 — tRNA
(tRNA-Lys(ctt))

BackyardAgain_165 — tRNA
(tRNA-Glu(ttc))

BackyardAgain_166 — tRNA
(tRNA-Gly(tcc))

BackyardAgain_167 — tRNA
(tRNA-Thr(cgt))

BackyardAgain_168 — tRNA
(tRNA-Thr(tgt))

BackyardAgain_169
(HNH endonuclease)

BackyardAgain_170 — tRNA
(tRNA-Thr(ggt))

BackyardAgain_171
(minor tail protein)

BackyardAgain_172

BackyardAgain_173 — tRNA
(tRNA-Gly(gcc))

BackyardAgain_174 — tRNA
(tRNA-Asp(gtc))

BackyardAgain_175 — tRNA
(tRNA-Met(cat))

BackyardAgain_176 — tRNA
(tRNA-Ile(gat))

BackyardAgain_177 — tRNA
(tRNA-Arg(acg))

BackyardAgain_178 — tRNA
(tRNA-Val(gac))

BackyardAgain_179 — tRNA
(tRNA-Arg(cct))

BackyardAgain_180 — tmRNA
(Tag peptide: ATDTDATVTDAEIEAFFAEEAAALV*)

BackyardAgain_181 — tRNA
(tRNA-Gln(ttg))

BackyardAgain_182 — tRNA
(tRNA-Arg(tct))

BackyardAgain_183 — tRNA
(tRNA-Lys(ttt))

BackyardAgain_184

BackyardAgain_185
(DNA helicase)

BackyardAgain_186

BackyardAgain_187

BackyardAgain_188

BackyardAgain_189

BackyardAgain_190

BackyardAgain_191

BackyardAgain_192

BackyardAgain_193

BackyardAgain_194
(thymidylate kinase)

BackyardAgain_195
(aminotransferase)

BackyardAgain_196

BackyardAgain_197

BackyardAgain_198
(DnaC-like helicase loader)

BackyardAgain_199
(DnaB-like dsDNA helicase)

BackyardAgain_200
(DNA primase)

BackyardAgain_201

BackyardAgain_202
(DnaJ-like chaperonin)

BackyardAgain_203
(ERF family ssDNA binding protein)

BackyardAgain_204
(DnaE-like DNA polymerase III)

BackyardAgain_205

BackyardAgain_206
(RF-1 peptide chain release factor)

BackyardAgain_207
(RecA-like DNA recombinase)

BackyardAgain_208
(holliday junction resolvase)

BackyardAgain_209

BackyardAgain_210
(Cas4 family exonuclease)

BackyardAgain_211
(RusA-like resolvase)

BackyardAgain_212

BackyardAgain_213

BackyardAgain_214

BackyardAgain_215

BackyardAgain_216

BackyardAgain_217

BackyardAgain_218

BackyardAgain_219

BackyardAgain_220 — tRNA
(tRNA-Gln(ctg))

BackyardAgain_221 — tRNA
(tRNA-Asn(gtt))

BackyardAgain_222

BackyardAgain_223

BackyardAgain_224

BackyardAgain_225

BackyardAgain_226

BackyardAgain_227

BackyardAgain_228
(Ro-like RNA binding protein)

BackyardAgain_229

BackyardAgain_230

BackyardAgain_231

BackyardAgain_232

BackyardAgain_233

BackyardAgain_234

BackyardAgain_235
(glycosyltransferase)

BackyardAgain_236

BackyardAgain_237
(glycosyltransferase)

BackyardAgain_238
(galactosyltransferase)

BackyardAgain_239
(PnuC-like nicotinamide riboside transporter)

BackyardAgain_240
(glycosyltransferase)

BackyardAgain_241

BackyardAgain_242

BackyardAgain_243

BackyardAgain_244

BackyardAgain_245
(lysin A)

BackyardAgain_246
(holin)

BackyardAgain_247
(lysin B)

BackyardAgain_248
(terminase)

BackyardAgain_249

BackyardAgain_250

BackyardAgain_251

BackyardAgain_252

BackyardAgain_253
(polynucleotide kinase)

BackyardAgain_254

BackyardAgain_255

BackyardAgain_256
(serine/threonine kinase)

BackyardAgain_257

BackyardAgain_258

BackyardAgain_259
(PurA-like adenylosuccinate synthetase)

BackyardAgain_260

BackyardAgain_261

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMT818416
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 103
Pitt Freezer Box Grid# A10
Available Files
GenBank File for PhameratorDownload