Mycobacterium phage Ewok
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Detailed Information for Phage Ewok
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByOlivia Kretlow
Year Found2016
Location FoundWaukegan, IL United States
Finding InstitutionCarthage College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates42.3954 N, 87.8269 W Map
Discovery NotesThe plants around the sample were alive and blooming. The mulch on top of the dirt has some white stuff that looked like really thick spiderwebs on top. I dug about 3 inches under the mulch and grabbed that dirt.
Naming NotesMy partner and I's initials spell EWOK (Erin Walz and Olivia Kretlow). Also our phage has a short tail. Since our initials spell Ewok we decided to name it that after the little creatures from Star Wars.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 24, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage551
Genome length (bp)155005
Character of genome endsCircularly Permuted
GC Content64.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Cluster Life CycleLytic
Other Cluster Members

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Boonesok (None)

CLach (None)

DavidMina (None)

DitchDweller (None)

Elpis (None)

Genet (None)

Hard2get (None)

Hippocrates (None)

Kali (None)

Linnaeus (None)

LittleSpot (None)

Lsquared (None)

Marlex (None)

Minipoke (None)

MoBama (None)

PeeWee (None)

Pesky7 (None)

Phomnie (None)

Phyllis (None)

Roku (None)

Sagamore (None)

Samarai (None)

ShenziUR (None)

SuperCallie (None)

TinyC (None)

Trilla23 (None)

Turret (None)

RetimsGems (A1)

Squidney (A3)

Ading (C1)

Adlitam (C1)

Alice (C1)

Amataga (C1)

ArcherS7 (C1)

Astraea (C1)

Atlantean (C1)

Atlas (C1)

Audrick (C1)

Ava3 (C1)

BadAgartude (C1)

Bangla1971 (C1)

Basquiat (C1)

BeanWater (C1)

Bigswole (C1)

Bipolarisk (C1)

Blackbrain (C1)

Bonray (C1)

Bread (C1)

Breeniome (C1)

Burrough (C1)

Bxz1 (C1)

Cactojaque (C1)

Cali (C1)

Cane17 (C1)

Catera (C1)

Chargie21 (C1)

CharlieB (C1)

ChickenPhender (C1)

Colt (C1)

Daffodil (C1)

Dandelion (C1)

Darko (C1)

Delilah (C1)

Derek (C1)

Dietrick (C1)

Donegal (C1)

Doodle1 (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Exxus285 (C1)

Flabslab (C1)

Fludd (C1)

FoxtrotP1 (C1)

FrayBell (C1)

FudgeTart (C1)

Gabriel (C1)

Ghost (C1)

Gizmo (C1)

Grungle (C1)

Guwapp (C1)

HarrySpotter (C1)

HyRo (C1)

InigoMontoya (C1)

InterFolia (C1)

Iota (C1)

Isee1101 (C1)

JennanaJr (C1)

Jessibeth14 (C1)

JustHall (C1)

Kamryn (C1)

Kboogie (C1)

Khaleesi (C1)

Koguma (C1)

LifeSavor (C1)

LinStu (C1)

Lionheart (C1)

Littleton (C1)

Loanshark (C1)

LolaVinca (C1)

LRRHood (C1)

Lukilu (C1)

McWolfish (C1)

Megamind (C1)

Melpomini (C1)

MePac (C1)

MikeLiesIn (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

MrsHay (C1)

Naija (C1)

Napoleon13 (C1)

Nappy (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

OaklaforG (C1)

ParkTD (C1)

Patch (C1)

Patter (C1)

Phlegm (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

QBert (C1)

Rabinovish (C1)

Rahel (C1)

Rizal (C1)

RoMag (C1)

Roots515 (C1)

Salacia (C1)

SandySandal (C1)

Sauce (C1)

ScottMcG (C1)

Sebastian (C1)

Sebata (C1)

ShankPowerGod (C1)

Shaqnato (C1)

Shelob (C1)

ShiaLabeouf (C1)

Shnickers (C1)

Shrimp (C1)

SmallFry (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

Stubby (C1)

Teardrop (C1)

TheO (C1)

Thuathua (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Trinitium (C1)

Tyke (C1)

ValleyTerrace (C1)

Wally (C1)

Willis (C1)

YoungMoneyMata (C1)

Yucca (C1)

Zalkecks (C1)

Zeenon (C1)

ZygoTaiga (C1)

Feauphix (C2)

Matcha (C2)

Myrna (C2)

Phabba (C2)

SimSimmers (C2)

SrishMeg2525 (C2)

Annotation StatusIn GenBank
Plaque NotesThe phage had large heads in a pentagon shape and they had short tails
MorphotypeMyoviridae
Has been Phamerated?Yes
Gene List

Click to View

Ewok_1

Ewok_2

Ewok_3
(HNH endonuclease)

Ewok_4

Ewok_5

Ewok_6

Ewok_7

Ewok_8

Ewok_9

Ewok_10

Ewok_11

Ewok_12

Ewok_13

Ewok_14

Ewok_15

Ewok_16

Ewok_17

Ewok_18
(histidine triad nucleotide binding protein)

Ewok_19

Ewok_20

Ewok_21

Ewok_22

Ewok_23

Ewok_24

Ewok_25

Ewok_26

Ewok_27

Ewok_28

Ewok_29

Ewok_30

Ewok_31
(helix-turn-helix DNA binding protein)

Ewok_32

Ewok_33

Ewok_34

Ewok_35
(ribbon-helix-helix DNA binding domain)

Ewok_36

Ewok_37

Ewok_38

Ewok_39

Ewok_40

Ewok_41

Ewok_42

Ewok_43

Ewok_44

Ewok_45

Ewok_46

Ewok_47

Ewok_48

Ewok_49

Ewok_50

Ewok_51

Ewok_52

Ewok_53

Ewok_54

Ewok_55

Ewok_56
(helix-turn-helix DNA binding protein)

Ewok_57

Ewok_58

Ewok_59

Ewok_60

Ewok_61

Ewok_62

Ewok_63
(helix-turn-helix DNA binding domain)

Ewok_64
(helix-turn-helix DNA binding domain)

Ewok_65

Ewok_66

Ewok_67

Ewok_68

Ewok_69

Ewok_70

Ewok_71

Ewok_72

Ewok_73

Ewok_74

Ewok_75

Ewok_76

Ewok_77

Ewok_78

Ewok_79

Ewok_80

Ewok_81

Ewok_82

Ewok_83

Ewok_84

Ewok_89
(methyltransferase)

Ewok_90

Ewok_91
(portal protein)

Ewok_92

Ewok_93

Ewok_94
(ThyX-like thymidylate synthase)

Ewok_95

Ewok_96
(RNA ligase)

Ewok_97
(LysM-domain)

Ewok_98

Ewok_99

Ewok_100
(major capsid protein)

Ewok_101
(Holliday junction resolvase)

Ewok_102
(capsid decoration protein)

Ewok_103

Ewok_104

Ewok_105

Ewok_106

Ewok_107

Ewok_108

Ewok_109
(membrane protein)

Ewok_110

Ewok_111

Ewok_112

Ewok_113

Ewok_114

Ewok_115

Ewok_116

Ewok_117

Ewok_118

Ewok_119

Ewok_120

Ewok_121

Ewok_122
(N-acetyltransferase)

Ewok_123

Ewok_124

Ewok_125

Ewok_126

Ewok_127

Ewok_128
(tail sheath protein)

Ewok_129

Ewok_130

Ewok_131
(tail assembly chaperone)

Ewok_132
(tail assembly chaperone)

Ewok_133
(tape measure protein)

Ewok_134
(minor tail protein)

Ewok_135

Ewok_136

Ewok_137
(minor tail protein)

Ewok_138

Ewok_139

Ewok_140
(baseplate wedge protein)

Ewok_141
(baseplate wedge protein)

Ewok_142

Ewok_143

Ewok_144

Ewok_145
(minor tail protein)

Ewok_146
(minor tail protein)

Ewok_147

Ewok_148

Ewok_149

Ewok_150

Ewok_151

Ewok_152

Ewok_153

Ewok_154

Ewok_157

Ewok_158
(peptidyl tRNA hydrolase)

Ewok_173
(minor tail protein, D-ala-D-ala carboxypeptidase)

Ewok_174

Ewok_186

Ewok_187
(helicase)

Ewok_188

Ewok_189

Ewok_190

Ewok_191

Ewok_192

Ewok_193

Ewok_194

Ewok_195

Ewok_196

Ewok_197
(thymidylate kinase)

Ewok_198
(aminotransferase)

Ewok_199
(RNA binding domain)

Ewok_200

Ewok_201
(DnaC-like helicase loader)

Ewok_202
(DnaB-like dsDNA helicase)

Ewok_203

Ewok_204
(DNA primase)

Ewok_205

Ewok_206
(chaperonin DnaJ-like)

Ewok_207
(ssDNA binding domain)

Ewok_208
(DNA polymerase III (alpha))

Ewok_209

Ewok_210
(RecA-like DNA recombinase)

Ewok_211
(Holliday junction resolvase)

Ewok_212

Ewok_213
(HNH endonuclease)

Ewok_214
(RusA-like resolvase)

Ewok_215

Ewok_216

Ewok_217

Ewok_218

Ewok_219
(glycoside hydrolase)

Ewok_220

Ewok_221

Ewok_224

Ewok_225

Ewok_226

Ewok_227

Ewok_228

Ewok_229

Ewok_230

Ewok_231

Ewok_232
(Ro-like RNA binding protein)

Ewok_233

Ewok_234

Ewok_235

Ewok_236

Ewok_237

Ewok_238

Ewok_239
(galactosaminyltransferase)

Ewok_240

Ewok_241
(glycosyltransferase)

Ewok_242
(galactosyltransferase)

Ewok_243
(nicotinate ribosyltransferase)

Ewok_244
(glycosyltransferase)

Ewok_245

Ewok_246

Ewok_247

Ewok_248

Ewok_249
(lysin A)

Ewok_250
(membrane protein)

Ewok_251
(lysin B)

Ewok_252
(terminase)

Ewok_253

Ewok_254

Ewok_255

Ewok_256

Ewok_257
(polynucleotide kinase)

Ewok_258

Ewok_259

Ewok_260
(serine/threonine kinase)

Ewok_261

Ewok_262

Ewok_263
(adenylosuccinate synthetase)

Ewok_264

Ewok_265

Ewok_266

Ewok_267

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK359314
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 33
Pitt Freezer Box Grid# A5
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload