| Detailed Information for Phage Alice |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Coreen M. Manley |
| Year Found | 2009 |
| Location Found | Aledo, TX USA |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 32.688207 N, 97.648082 W Map |
| Discovery Notes | Found in a compost pile in a garden. The pile had been there for about 6 months so lots of decaying matter, mainly from fruit and vegetables. The soil was damp and it was moderately warm compared with the air temperature. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jul 29, 2010 |
| Genome length (bp) | 153401 |
| Character of genome ends | Circularly Permuted |
| GC Content | 64.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | C |
| Subcluster | C1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | 1mm and 1.5mm plaques, very clear, 2 sizes
24--36 hour incubation period in 37 degrees Celsius conditions
|
| Morphotype | Myoviridae |
| Number of Genes | 221 |
| Number of tRNAs | 29 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | JF704092 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
11.9e10 |
| Pitt Freezer Box# |
9 |
| Pitt Freezer Box Grid# |
45 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| Virtual Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
