| Detailed Information for Phage Asterius |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Tobias Shaw |
| Year Found | 2023 |
| Location Found | Columbus, OH United States |
| Finding Institution | Hope College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 34°C |
| GPS Coordinates | 40.1 N, 83.0 W Map |
| Discovery Notes | Collected from soil underneath tomato plant roots in my backyard. |
| Naming Notes | Asterius is the Minotaur of the Labyrinth in Greek mythology. He's quite an interesting character, and I thought that he should be represented in the phage database. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 2, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 525 |
| Genome length (bp) | 154871 |
| Character of genome ends | Circularly Permuted |
| GC Content | 64.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | C |
| Subcluster | C1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Hope College |
| Annotation Status | Awaiting_QC |
| Plaque Notes | After 48 hours at 34 degrees Celcius, Asterius plaques show two distinct morphologies: one larger comet tail plaque type with a clear center and then increasingly turbid ring/tail, and a smaller pinprick plaque type with a clear center and turbid ring. The larger plaques have a diameter of about 1.5mm, and the smaller pinpricks have a diameter of about 0.3-0.5mm. |
| Morphotype | Myoviridae |
| Number of Genes | 230 |
| Number of tRNAs | 33 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
182 |
| Pitt Freezer Box Grid# |
F2 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
