Detailed Information for Phage Astraea |
Discovery Information |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Ammu Vijay |
Year Found | 2011 |
Location Found | Raleigh, NC USA |
Finding Institution | North Carolina State University |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | Not entered |
GPS Coordinates | 35.782178 N, 78.666092 W Map |
Discovery Notes | Soil from garden near Bagwell Hall. 74 degrees F. Collected on August 21, 2011 at 8:40pm. It was humid and drizzling outside. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Jan 29, 2012 |
Sequencing Facility | David H. Murdock Research Institute |
Shotgun Sequencing Method | 454 Pyrosequencing |
Approximate Shotgun Coverage | 150 |
Genome length (bp) | 154872 |
Character of genome ends | Circularly Permuted |
GC Content | 64.7% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | C |
Subcluster | C1 |
Cluster Life Cycle | Lytic |
Other Cluster Members |
|
Annotating Institution | Unknown or unassigned |
Annotation Status | In GenBank |
Plaque Notes | Plaques get to be 2 mm if left in incubator for 48 hours, but are 0.5 mm if left in incubator for 24 hours. Plaques are always clear and circular. Host range only includes types of M. smegmatis bacteria. |
Morphotype | Myoviridae |
Number of Genes | 232 |
Number of tRNAs | 31 |
Number of tmRNAs | 1 |
Has been Phamerated? | Yes |
Gene List |
|
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | KC691257 |
Refseq Number | NC_021349 |
Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
Archiving Info |
Archiving status |
Archived |
SEA Designator |
2011NCSUAstraeaAAV |
Pitt Freezer Box# |
26 |
Pitt Freezer Box Grid# |
30 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |