Mycobacterium phage BeanWater
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Detailed Information for Phage BeanWater
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Duplicates of this PhageGayleyAve
Found ByTeam Pirates of the CUREibbean
Year Found2012
Location FoundLos Angeles, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramUnavailable
From enriched soil sample?Yes
Isolation Temperature35°C
GPS Coordinates34.075 N, 118.44 W Map
Discovery NotesDiscovered in spring 2012 by Team Pirates of the CURE-ibbean: Tiffany Chow, Josephine Nguyen, Sean Chang, and Khristina Ipapo.

BeanWater was isolated from an enrichment culture inoculated with a water sample from Stone Canyon Creek behind the UCLA Anderson School of Management. The sample was collected on April 4, 2012, by another student in the same class, Nick Morales.

DNA preparation was performed by Dr. Krisanavane Reddi (UCLA). The genome was assembled by William Villella (UCLA) and the assembly was curated by Dan Russel from the University of Pittsburgh.

Beanwater was annotated in Fall of 2013 by Teams: Fly Like a T4 (Amrit Dosanjh, Jaskirat Dhanoa, Jasmine Gajeton, Alex Tonthat), Tranducers ( Conny Tran, Stephanie Vu, Cheng Tian, Kyoko Yamaguchi), and Where the Wild Things Are. The genome was curated in Spring 2014 by Amrit Dosanjh and Jordan Meyer.
Naming NotesFormerly named CapNCrunch. GayleyAve is a duplicate of BeanWater and was unfortunately isolated as a contaminant.

Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedSep 18, 2013
Sequencing FacilityThe UCLA Genotyping and Sequencing Core
Shotgun Sequencing Method454 Pyrosequencing
Approximate Shotgun Coverage32
Genome length (bp)154061
Character of genome endsCircularly Permuted
GC Content64.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterC
SubclusterC1
Cluster Life CycleLytic
Other Cluster Members

Click to View

Ading (C1)

Adlitam (C1)

Alice (C1)

Amataga (C1)

ArcherS7 (C1)

Asterius (C1)

Astraea (C1)

Atlantean (C1)

Audrick (C1)

Ava3 (C1)

Babyland (C1)

BackyardAgain (C1)

BadAgartude (C1)

BananaFence (C1)

Bangla1971 (C1)

Basquiat (C1)

BeanWater (C1)

BigCity (C1)

Bigswole (C1)

Bipolarisk (C1)

Blackbrain (C1)

Blackdragon (C1)

Blubba (C1)

Bonray (C1)

Bread (C1)

Breeniome (C1)

Burrough (C1)

Bxz1 (C1)

Cactojaque (C1)

Cali (C1)

Cane17 (C1)

Capablanca (C1)

Caravan (C1)

Catera (C1)

Chargie21 (C1)

CharlieB (C1)

ChaylaJr (C1)

ChickenPhender (C1)

Chomp (C1)

CindyLou (C1)

Colt (C1)

Concombre (C1)

Daffodil (C1)

Dandelion (C1)

Darko (C1)

Delilah (C1)

Delylah (C1)

Demodog (C1)

Derek (C1)

Dietrick (C1)

DirtMonster (C1)

Domi (C1)

Drazdys (C1)

DrPhinkDaddy (C1)

DTDevon (C1)

EasyJones (C1)

EggyFarm (C1)

EmmaElysia (C1)

EmToTheThree (C1)

Erdmann (C1)

ErnieJ (C1)

Essence (C1)

ET08 (C1)

Ewok (C1)

Flabslab (C1)

Fludd (C1)

FoxtrotP1 (C1)

FrayBell (C1)

FudgeTart (C1)

Gabriel (C1)

Gamgam (C1)

Ghost (C1)

Gizmo (C1)

Grasshills (C1)

Grungle (C1)

Guwapp (C1)

Halldule (C1)

HyRo (C1)

I3 (C1)

IkeLoa (C1)

InigoMontoya (C1)

InterFolia (C1)

Iota (C1)

Issimir (C1)

Izajani (C1)

Janiyra (C1)

Jaygup (C1)

JayJay (C1)

Jedediah (C1)

Jemmno (C1)

Jessibeth14 (C1)

JigglyPuff (C1)

JPickles (C1)

JulietS (C1)

JustHall (C1)

Kamryn (C1)

Kboogie (C1)

Khaleesi (C1)

Koguma (C1)

Latch (C1)

Lee7 (C1)

LifeSavor (C1)

LinStu (C1)

Littleton (C1)

Loanshark (C1)

LolaVinca (C1)

LordLeafolot (C1)

LRRHood (C1)

Lukilu (C1)

Maby2021 (C1)

Mangeria (C1)

Matsumoto (C1)

McGee (C1)

McWolfish (C1)

Megamind (C1)

Melpomini (C1)

MikeLiesIn (C1)

Mikro (C1)

Momo (C1)

MoMoMixon (C1)

Morizzled23 (C1)

Naija (C1)

Napoleon13 (C1)

Nappy (C1)

Naval22 (C1)

NedWong (C1)

Ngannou (C1)

Nidhogg (C1)

NoodleTree (C1)

Norm (C1)

NuevoMundo (C1)

ParkTD (C1)

Patter (C1)

Phlegm (C1)

Phox (C1)

Phusco (C1)

Pier (C1)

Pinkcreek (C1)

Pio (C1)

Pivoine (C1)

Pleione (C1)

PurduePete (C1)

QBert (C1)

Quasimodo (C1)

Rabinovish (C1)

Rahel (C1)

Rizal (C1)

RoMag (C1)

Ronan (C1)

Roots515 (C1)

Salacia (C1)

Sauce (C1)

ScottMcG (C1)

Sebata (C1)

Shaqnato (C1)

Shelob (C1)

ShiaLabeouf (C1)

Shifa (C1)

Shnickers (C1)

Shrimp (C1)

SilverDipper (C1)

SmallFry (C1)

Spec (C1)

Specks (C1)

Sprinklers (C1)

Spud (C1)

SrishMeg2525 (C1)

StephanieG (C1)

Stubby (C1)

StubbySaigey (C1)

Tainan (C1)

Teardrop (C1)

TinyTim (C1)

Tonenili (C1)

Tortoise16 (C1)

Trinitium (C1)

Turret (C1)

Tyke (C1)

ValleyTerrace (C1)

Wally (C1)

Willis (C1)

Yassified (C1)

YemiJoy2021 (C1)

YoungMoneyMata (C1)

Yucca (C1)

Zalkecks (C1)

Zeenon (C1)

ZygoTaiga (C1)

Greely (C2)

Myrna (C2)

Phabba (C2)

ScoobyDoobyDoo (C2)

Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesClear plaques of 1-2 mm diameter.
MorphotypeMyoviridae
Number of Genes225
Number of tRNAs34
Number of tmRNAs1
Has been Phamerated?Yes
Gene List

Click to View

BeanWater_1

BeanWater_2

BeanWater_3
(nucleotidyltransferase)

BeanWater_4

BeanWater_5

BeanWater_6

BeanWater_7

BeanWater_8

BeanWater_9

BeanWater_10

BeanWater_11

BeanWater_12

BeanWater_13

BeanWater_14

BeanWater_15

BeanWater_16

BeanWater_17

BeanWater_18

BeanWater_19

BeanWater_20

BeanWater_21
(HNH endonuclease)

BeanWater_22

BeanWater_23

BeanWater_24

BeanWater_25
(band-7-like membrane protein)

BeanWater_26

BeanWater_27

BeanWater_28

BeanWater_29

BeanWater_30

BeanWater_31

BeanWater_32

BeanWater_33

BeanWater_34

BeanWater_35

BeanWater_36

BeanWater_37

BeanWater_38

BeanWater_39

BeanWater_40

BeanWater_41

BeanWater_42

BeanWater_43

BeanWater_44

BeanWater_45

BeanWater_46

BeanWater_47

BeanWater_48

BeanWater_49

BeanWater_50

BeanWater_51

BeanWater_52

BeanWater_53

BeanWater_54

BeanWater_55

BeanWater_56
(helix-turn-helix DNA binding domain protein)

BeanWater_57
(helix-turn-helix DNA binding protein)

BeanWater_58

BeanWater_59

BeanWater_60

BeanWater_61

BeanWater_62

BeanWater_63

BeanWater_64

BeanWater_65

BeanWater_66

BeanWater_67

BeanWater_68

BeanWater_69

BeanWater_70

BeanWater_71

BeanWater_72

BeanWater_73

BeanWater_74

BeanWater_75

BeanWater_76 — tRNA
(tRNA-Ser(gct))

BeanWater_77 — tRNA
(tRNA-Leu(cag))

BeanWater_78 — tRNA
(tRNA-Leu(gag))

BeanWater_79 — tRNA
(tRNA-Leu(caa))

BeanWater_80

BeanWater_81

BeanWater_82

BeanWater_83

BeanWater_84

BeanWater_85
(ThyX-like thymidylate synthase)

BeanWater_86

BeanWater_87
(RNA ligase)

BeanWater_88

BeanWater_89

BeanWater_90

BeanWater_91
(major capsid protein)

BeanWater_92

BeanWater_93

BeanWater_94

BeanWater_95

BeanWater_96

BeanWater_97

BeanWater_98

BeanWater_99

BeanWater_100

BeanWater_101

BeanWater_102

BeanWater_103

BeanWater_104

BeanWater_105

BeanWater_106

BeanWater_107

BeanWater_108

BeanWater_109

BeanWater_110

BeanWater_111

BeanWater_112

BeanWater_113

BeanWater_114

BeanWater_115

BeanWater_116

BeanWater_117

BeanWater_118

BeanWater_119
(tail sheath protein)

BeanWater_120

BeanWater_121

BeanWater_122
(tail assembly chaperone)

BeanWater_123
(tail assembly chaperone)

BeanWater_124
(tape measure protein)

BeanWater_125

BeanWater_126

BeanWater_127

BeanWater_128
(minor tail protein)

BeanWater_129

BeanWater_130
(minor tail protein)

BeanWater_131
(baseplate wedge protein)

BeanWater_132
(baseplate J protein)

BeanWater_133

BeanWater_134

BeanWater_135

BeanWater_136

BeanWater_137
(minor tail protein)

BeanWater_138

BeanWater_139

BeanWater_140

BeanWater_141

BeanWater_142

BeanWater_143

BeanWater_144

BeanWater_145 — tRNA
(tRNA-Pro(tgg))

BeanWater_146 — tRNA
(tRNA-Trp(cca))

BeanWater_147 — tRNA
(tRNA-Tyr(gta))

BeanWater_148

BeanWater_149
(peptidyl tRNA hydrolase)

BeanWater_150 — tRNA
(tRNA-Pyl (cta))

BeanWater_151 — tRNA
(tRNA-Met (cat))

BeanWater_152 — tRNA
(tRNA-Cys (gca))

BeanWater_153 — tRNA
(tRNA-Gln (ctc))

BeanWater_154 — tRNA
(tRNA-His (gtg))

BeanWater_155 — tRNA
(tRNA-Ala (tgc))

BeanWater_156 — tRNA
(tRNA-Phe (gaa))

BeanWater_157 — tRNA
(tRNA-Val (cac))

BeanWater_158 — tRNA
(tRNA-Lys (ctt))

BeanWater_159 — tRNA
(tRNA-Glu (ttc))

BeanWater_160 — tRNA
(tRNA-Gly (tcc))

BeanWater_161 — tRNA
(tRNA-Thr (cgt))

BeanWater_162 — tRNA
(tRNA-Thr (tgt))

BeanWater_163 — tRNA
(tRNA-Thr (ggt))

BeanWater_164
(minor tail protein)

BeanWater_165

BeanWater_166 — tRNA
(tRNA-Gly (gcc))

BeanWater_167 — tRNA
(tRNA-Asp (gtc))

BeanWater_168 — tRNA
(tRNA-Met (cat))

BeanWater_169 — tRNA
(tRNA-Ile (gat))

BeanWater_170 — tRNA
(tRNA-Arg (acg))

BeanWater_171 — tRNA
(tRNA-Val (gac))

BeanWater_172 — tRNA
(tRNA-Gly (tcc))

BeanWater_173 — tRNA
(tRNA-Arg (cct))

BeanWater_174 — tmRNA
(tmRNA)

BeanWater_175 — tRNA
(tRNA-Gln (ttg))

BeanWater_176 — tRNA
(tRNA-Arg (tct))

BeanWater_177 — tRNA
(tRNA-Lys (ttt))

BeanWater_178

BeanWater_179
(DNA helicase)

BeanWater_180

BeanWater_181

BeanWater_182

BeanWater_183

BeanWater_184

BeanWater_185

BeanWater_186

BeanWater_187

BeanWater_188

BeanWater_189
(thymidylate kinase)

BeanWater_190
(aminotransferase)

BeanWater_191

BeanWater_192

BeanWater_193

BeanWater_194
(DnaC-like helicase loader)

BeanWater_195
(DnaB-like dsDNA helicase)

BeanWater_196

BeanWater_197
(DNA primase)

BeanWater_198

BeanWater_199
(DnaJ-like chaperonin)

BeanWater_200

BeanWater_201
(DnaE-like DNA polymerase III)

BeanWater_202
(RF-1 peptide chain release factor)

BeanWater_203
(RecA-like DNA recombinase)

BeanWater_204
(holliday junction resolvase)

BeanWater_205

BeanWater_206
(Cas4 family exonuclease)

BeanWater_207
(RusA-like resolvase)

BeanWater_208

BeanWater_209

BeanWater_210

BeanWater_211

BeanWater_212

BeanWater_213

BeanWater_214
(HNH endonuclease)

BeanWater_215

BeanWater_216 — tRNA
(tRNA-Gln (ctg))

BeanWater_217 — tRNA
(tRNA-Asn (gtt))

BeanWater_218

BeanWater_219

BeanWater_220

BeanWater_221

BeanWater_222

BeanWater_223

BeanWater_224

BeanWater_225

BeanWater_226
(Ro-like RNA binding protein)

BeanWater_227

BeanWater_228

BeanWater_229

BeanWater_230

BeanWater_231

BeanWater_232

BeanWater_233
(glycosyltransferase)

BeanWater_234

BeanWater_235
(glycosyltransferase)

BeanWater_236
(glycosyltransferase)

BeanWater_237
(PnuC-like nicotinamide riboside transporter)

BeanWater_238
(glycosyltransferase)

BeanWater_239

BeanWater_240

BeanWater_241

BeanWater_242

BeanWater_243
(lysin A)

BeanWater_244
(holin)

BeanWater_245
(lysin B)

BeanWater_246
(terminase)

BeanWater_247

BeanWater_248

BeanWater_249

BeanWater_250

BeanWater_251
(polynucleotide kinase)

BeanWater_252

BeanWater_253

BeanWater_254
(serine/threonine kinase)

BeanWater_255

BeanWater_256

BeanWater_257
(PurA-like adenylosuccinate synthetase)

BeanWater_258

BeanWater_259

BeanWater_260

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF919494
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
SEA Lysate Titer Archived as CapnCrunch
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload