| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Elena Baggett |
| Year Found | 2022 |
| Location Found | Bowling Green, KY United States |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 30°C |
| GPS Coordinates | 36.949722 N, 86.466667 W Map |
| Discovery Notes | My phage is from a water sample obtained from the 1st blue hole at Lost River Cave. I collected the water from an area that had floating brown specks on the waters surface, and small debris. Area contained trees, and there were leaves along with a fallen log in the water. |
| Naming Notes | When I first saw what bacteriophage looked like, I thought the capsid heads resembled little blobs, and figured Blubba would be a suiting name. After my EM pictures, I definitely agree that it's fitting. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Apr 7, 2023 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1180 |
| Genome length (bp) | 158907 |
| Character of genome ends | Circularly Permuted |
| GC Content | 64.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | C |
| Subcluster | C1 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | My plaques were clear and see-through (especially under light), meaning my phage is lytic. |
| Other Cluster Members |
|
| Annotating Institution | Western Kentucky University |
| Annotation Status | In GenBank |
| Plaque Notes | My phage is lytic and produces small clear plaques about 1.4 cm in diameter. |
| Morphotype | Myoviridae |
| Number of Genes | 222 |
| Number of tRNAs | 30 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PQ244016 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.3 x 10^10 |
| Pitt Freezer Box# |
157 |
| Pitt Freezer Box Grid# |
F2 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
